LOCUS       NZ_CP138396            16837 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Escherichia coli strain 101EVA plasmid p101EVA-3, complete sequence.
ACCESSION   NZ_CP138396
VERSION     NZ_CP138396.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38124879
            Assembly: GCF_033802405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16837)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Tet(X4) producing Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16837)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) Henning Seedorf Group, Temasek Life Sciences
            Laboratory Limited, 1 Research Link National University of Singapore
            Singapore, Singapore 117604, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Nanopolish v. 0.13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 437x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 16:18:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,788
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,490
            CDSs (with protein)               :: 4,490
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 72 of 181
            Pseudo Genes (incomplete)         :: 98 of 181
            Pseudo Genes (internal stop)      :: 38 of 181
            Pseudo Genes (multiple problems)  :: 29 of 181
            Pseudo Genes (short protein)      :: 3 of 181
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138396.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..16837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="101EVA"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p101EVA-3"
                     /geo_loc_name="Singapore"
                     /collection_date="2019-01"
     gene            complement(join(16680..16837,1..7))
                     /locus_tag="SE696_RS23865"
     CDS             complement(join(16680..16837,1..7))
                     /locus_tag="SE696_RS23865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319776578.1"
                     /translation="MMRNQLKEQHNGIMEYDPNDRQDQSKIFFLDLSKHEGITNEIIEE
                     LKELKNPMT"
                     /name="hypothetical protein"
     gene            complement(1235..1525)
                     /locus_tag="SE696_RS23870"
     CDS             complement(1235..1525)
                     /locus_tag="SE696_RS23870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319776579.1"
                     /translation="MINHVNDDLQCCFSKPSAKVLVVRSDGFPLPRSLLTPFSHESLRQ
                     VEEIMDVSSSVKLIMGNLHEFYGEVKRAAIKSVTSKPYPTPFKISTIELVL"
                     /name="hypothetical protein"
     gene            2374..2505
                     /locus_tag="SE696_RS23875"
     CDS             2374..2505
                     /locus_tag="SE696_RS23875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319776580.1"
                     /translation="MHAVKQSLRVSGGFSGGLLPVLPVSGHETRSGRLSGARNATLW"
                     /name="hypothetical protein"
     gene            2678..3121
                     /locus_tag="SE696_RS23880"
     CDS             2678..3121
                     /locus_tag="SE696_RS23880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(3419..4297)
                     /locus_tag="SE696_RS23885"
     CDS             complement(3419..4297)
                     /locus_tag="SE696_RS23885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319776581.1"
                     /translation="MRFRRKAEKKTIYKVTSSDDKFFRNQLKEQHNGIMEYDPNDRQDQ
                     SKIFFLDLSKHEGITNEIIEELKELKKSDDINTTNYKAFSASDLDGLKYMATKQDQYIL
                     FQNFYKKNIIGSKVLSLKKMEVLQDGFITLNEYAEVIYDIEDKKIYFSNIVKAKKILSG
                     MEMIYKEAGIKETKHFISQDHIHLLDDFDENKVNVLNKKRIQSELDKQNKIKVEKGDDY
                     DDFYTTDISECLAEIKEYYPEMLDGNKIIIKNNKDLENYLLALDERLYTAKRSQQQRVA
                     TATRKVEKSKQ"
                     /name="hypothetical protein"
     gene            5722..5913
                     /locus_tag="SE696_RS23890"
     CDS             5722..5913
                     /locus_tag="SE696_RS23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6887..7330
                     /locus_tag="SE696_RS23895"
     CDS             6887..7330
                     /locus_tag="SE696_RS23895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(7634..8161)
                     /locus_tag="SE696_RS23900"
     CDS             complement(7634..8161)
                     /locus_tag="SE696_RS23900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319776574.1"
                     /translation="MLSLKKMEVLQDGFITLNEYAEVIYDIEDKKIYFSNIVKAKKILS
                     GMEMIYKEAGIKETKHFISQDHIHLLDDFDENKVNVLNKKRIQSELDKQNKIKVEKGDD
                     YDDFYTTDISECLAEIKEYYPEMLDGNKIIIKNNKDLENYLLALDERLYTAKRSQQQRV
                     ATATRKVEKSKQ"
                     /name="hypothetical protein"
     gene            complement(8149..8529)
                     /locus_tag="SE696_RS23905"
     CDS             complement(8149..8529)
                     /locus_tag="SE696_RS23905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222214856.1"
                     /translation="MKKNHLIAFSQKSRKKTIYKVTSSDDKFFRNQLKEQHNGIMEYDP
                     NDRQDQSKIFFLDLSKHEGITNEIIEELKELKKSDDINTTNYKAFSASDLDGLKYMATK
                     QDQYILFQNFYKKISLVQRCCL"
                     /name="hypothetical protein"
     gene            9930..10121
                     /locus_tag="SE696_RS23910"
     CDS             9930..10121
                     /locus_tag="SE696_RS23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            11093..11536
                     /locus_tag="SE696_RS23915"
     CDS             11093..11536
                     /locus_tag="SE696_RS23915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(11838..12365)
                     /locus_tag="SE696_RS23920"
     CDS             complement(11838..12365)
                     /locus_tag="SE696_RS23920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319776574.1"
                     /translation="MLSLKKMEVLQDGFITLNEYAEVIYDIEDKKIYFSNIVKAKKILS
                     GMEMIYKEAGIKETKHFISQDHIHLLDDFDENKVNVLNKKRIQSELDKQNKIKVEKGDD
                     YDDFYTTDISECLAEIKEYYPEMLDGNKIIIKNNKDLENYLLALDERLYTAKRSQQQRV
                     ATATRKVEKSKQ"
                     /name="hypothetical protein"
     gene            complement(13566..13714)
                     /locus_tag="SE696_RS23925"
                     /pseudo=""
     CDS             complement(13566..13714)
                     /locus_tag="SE696_RS23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            14130..14321
                     /locus_tag="SE696_RS23930"
     CDS             14130..14321
                     /locus_tag="SE696_RS23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_319776575.1"
                     /translation="MNKQQKTAVNMAKFIGDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            15292..15735
                     /locus_tag="SE696_RS23935"
     CDS             15292..15735
                     /locus_tag="SE696_RS23935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(15917..16558)
                     /locus_tag="SE696_RS23940"
     CDS             complement(15917..16558)
                     /locus_tag="SE696_RS23940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319776577.1"
                     /translation="MLSLKKMEVLQDGFITLNEYAEVIYDIEDKKIYFSNIVKAKKILS
                     GMEMIYKEAGIKETKHFISQDHIHLLDDFDENKVNVLNKKRIQSELDKQNKIKVEKGDD
                     YDDFYTTDISECLAEIKEYYPEMLDGNKIIIKNNKDLENYLLALDERLYTAKRSQQQRV
                     ATATRKVENQNNRWKPNPNRKLKNHARLFLSKSILRITRTAGLRRFLYKK"
                     /name="hypothetical protein"
     Typing          complement(5225..5847)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.555"
                     /coverage="99.0"
                     /evalue="1.99e-167"
     Typing          complement(9432..10055)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.424"
                     /coverage="99.0"
                     /evalue="2.57e-166"
     Typing          complement(13638..14255)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.775"
                     /coverage="99.0"
                     /evalue="5.6e-158"
ORIGIN
        1 tcatcatctg aagatgctgg ctttgtagat ggtcttttcc gctatgttct gcgaaacgca
       61 ataaggtggt tttttttcat tttactctac cttgatgaat gtgaagtcat ttattctttt
      121 ttatagttgg aggatgagat gtcttatgag aacggagatt catccttact tataagtaat
      181 atttacaccc ttatcagatg ttaagagata gaatttaaac ccgacattaa gagtgacgga
      241 ttaaaaaata taattttgag aaaaggttga ataggagaac gagaaccagg gcaaacaaaa
      301 acccagtctg atgcaatgct aactgagaca aaaaaatacc ataagtatga cggaaaaaaa
      361 tcatcattaa ccggctacac tgtatttata ttgctcatcg actcaacatc aaggttgtta
      421 actacgcgca agaatattga attaagaaac agaactatga gtacgcttaa aatgatgcag
      481 gcgatgaaaa tatagcttca ccatcagaga cataatagtt acttttcagc tctctttatt
      541 aaggtttgtt ttgtagtttg tgatctatag gcacacaaat attggttgag ttaatgccag
      601 acattgtaga taataattta aaagacataa tcgagtatgt ctgcggaatt tattttattg
      661 taacacagtg ttctcccaga tgttacagtg ctgggtatct ttcattttgc acaactttgc
      721 tatatggcaa tgaatgctgg gtaggtaggc tgaaatgctg actactgcat aagtcacagc
      781 gtactctctt cttttgaaag aaaaaccaac caggcagaaa ctttgccaaa ggttagaaaa
      841 tcccggcaga tgttctaacc gtggtaacag aactatacga gactgtcact aaattatcaa
      901 ttgtagtttt atcacagcag agccaccttc agaacccata tccagacttt actaccaatg
      961 tatgcatata tgttttatta gttttccata gactaatagg cagcgccact gagatcaagg
     1021 cgcagcgcgg cttgatcgaa cgaatcgtgg tggcggctgt ctgcttgata tgaaccgctc
     1081 aactggaacc gataaaaggc ggcgttggtt cgagatgcac cgccgtgaag agaggcggca
     1141 tccatatcgt aaaaccaggt ggcggttgcg agagacaaca ggagccaaac cggtgggaaa
     1201 cgctcgcatc tactgcagat caaccaatga tcccttacaa caccagttcg attgtggaga
     1261 ttttgaaagg tgttgggtat ggtttgctgg taactgactt tatggcggct cttttcactt
     1321 ctccatagaa ctcatgcagg tttcccatga taagtttaac tgaagatgat acgtccatga
     1381 tctcctccac ctgccgcaga ctttcatgac tgaacggagt gagcaacgag cgaggaagcg
     1441 gaaaaccatc agaacgtaca acaagcactt tcgcgctggg cttgctgaaa caacactgca
     1501 gatcgtcgtt aacatggtta atcatccgcg atcagtcccc gcttcttctg taaagacatg
     1561 tgcttaacag gttgattcga gacggcgagg ctgatttcag acaactcctg aggaactgct
     1621 gagtggtaga ccgcctttat gaagcttctg gtcgcgcggt cgcaacggca atgatatggc
     1681 accgctagat cgagttggtg ctgagatgac actcggtggc gactgcgtgc aacgccatcg
     1741 cttcaattcg cgtgtttgaa ggagcattta atattgctat cctcagcaca accagaatcc
     1801 cgtggtttct gagttgtagc acattccgga gttatgatga aaatcgcagt tgcgttaggt
     1861 gtatgtcatt ttttagtata taaaacgacc actcccgagt gctggcgccg caggctaagt
     1921 gatgattctg gctaaaaagc gtcctttttc cgggaaaggg tcatgctcgt atgaccctat
     1981 gggttcgagc gactacaaca taaactacaa aattgcatct gcttttaccc cagaacgcgg
     2041 gtgtagcaac agaattacat caaaagtaca tcaaaattac atcactttta cgtcagatcg
     2101 tgggtgtaca acagaattac atcaaaagta catcactttt acatcatttt tacgtcagat
     2161 ctgacctctg agctattcgt acatccgccc ctcttccgtg gcctcctgca cgtgctgaga
     2221 atggtttttc tggctgtgct ggcaacgctt cacggccacg cacaaattgc gtccccgttc
     2281 accgttttcg ggtaagaaga acatcggaaa caggacttac cccggctgaa tggcgtgaat
     2341 ttccatgcgc tatgcacttg tgcgcaatac atcatgcatg ccgtaaaaca gagcctgcgc
     2401 gtctctggcg ggttttcggg tggtttgttg cccgttttac cggtttccgg tcatgaaacg
     2461 cgctcaggcc gtctgagcgg tgcgcgtaat gcgacgttat ggtaaatccg ggctgttgag
     2521 tgttgacctc tcactatcgc aacccagtta atgcggtaaa tcaaacacaa ctagctgtta
     2581 attgatgtta gttgtattta cttgtcactt gtcttgttga caactatctt gtctatgttt
     2641 attctccttt tatcttaaca agacaatgga gtgtttcatg actagtggta ttttcttatc
     2701 ccgtgaagat ttgatgttgt tatcagacca agcaaaagaa gaagtgctgg cattggctac
     2761 tggggattcg gtttctggtg tttatgagga gtcggaagat gggcctactg atctttcctc
     2821 gcttcaggca gaaaggcttg ttcgtggttt aagtgataag tcgcgaacag tgctgaaagc
     2881 tattcttgaa agcgaggatg gtgagcgagg attctggtgc aagagcctcg caaaggagct
     2941 tggttttgat gtctctgagc tttcaggtgt ctggtcaggg cttacacgca gaactcgcac
     3001 ggtaagtgat gattctgagg cttatttgat tagctgggct tgggatgatg accgtgatga
     3061 ttactatgga acgctacatc caacaaccta tcgcaactgc aagaaagcta tgaacttttg
     3121 attgttctcg ctgtgatact tttcctgtga gcccgccatg tgcgggcttt ttcattggtt
     3181 cgtgtagtga gacgttatgg taaatcctcg aataataaaa gcccccagtg gaggcttttg
     3241 tgtgtgggca ctaggaaaac aaaccatgct aagaggattg aatttaacga ctgattccgc
     3301 tctatttctt gtacagaaat cgccgtagcc cagccgttct ggtgatacgt aaaatcgatt
     3361 tgctccaaaa atagtcttgc atggtttttt tagcttccta tttgggttgg gtttccatct
     3421 attgttttga tttttctact tttcttgttg ctgttgctac gcgctgttgc tggcttcttt
     3481 tcgctgtgta caagcgttca tctaaagcta acagatagtt ttctagatct ttattatttt
     3541 taataataat tttattccca tctaacatct ctggatagta ttcttttatt tcagctagac
     3601 attctgatat gtcggtagtg taaaaatcat cataatcatc acctttttca acttttattt
     3661 tattttgttt atctaattct gattgtattc tctttttatt taatacgttt actttatttt
     3721 catcgaaatc atctaataga tgaatgtgat cttggctaat gaaatgtttt gtttctttta
     3781 ttcctgcttc tttatatatc atttccattc cggatagaat tttcttagct ttaacaatat
     3841 ttgaaaaata aattttttta tcttctatat cataaattac ctctgcatat tcatttagtg
     3901 tgataaatcc gtcttgtaat acctccattt tttttaaaga cagcaccttt gaaccaatga
     3961 tatttttttt ataaaagttt tggaacaaaa tatattgatc ttgttttgtc gccatgtatt
     4021 ttaaaccatc aagatcacta gcggaaaatg ccttgtaatt agttgtgttt atgtcatcgg
     4081 atttttttaa ttcctttaat tcttcaatta tttcgttagt gataccttcg tgtttagaga
     4141 ggtctaaaaa gaatattttg ctttgatctt gcctgtcatt tggatcatac tccataattc
     4201 cattatgttg ttcttttagt tgatttctaa agaatttatc atctgaagat gttactttgt
     4261 agatggtttt tttttctgct tttctgcgaa aacgcaataa ggtggttttt tttcatttta
     4321 ctctaccttg atgaatgtga agtcatttat tcttttatag ttggaggatg agatgtcttt
     4381 atgagaacgg agatcatcct tacttataag taatattttt actcctttat cagatgttaa
     4441 gtgatagaat ttaaaacctg atattaatag tgacggatta aaaaaaatat acttttgaga
     4501 aaaggttgaa taggataacg agaatcataa agtagccaaa aacccagttt gatgtaatgc
     4561 taactgagac aaaaaaatac cctaagtatg tcggaagaaa attatcatta accggctcca
     4621 ctgtatttat attgctcatt gactcatcgt caagtttgtt aactacgcgc aagaatattg
     4681 aattaagaaa cagaattatg agtacgctta aaatgatgta ggctatgaaa aacatagctt
     4741 cattatcaga gataatagtt actagctctt tattaaggtt tgttttgtag tttgtgatca
     4801 tgtacacaaa tattgctgga gttaatgctg atattgtaga taataattta aagacataat
     4861 cgagtatgtc tgtggaattt attttattta acacagtgtt ctcccgtgac aaaggctttt
     4921 ttattttaaa ttttgctata tgtcaataaa taaatgccgg gtaggtaggc ttggaaatgt
     4981 tgactactgc attagtgaca gcgtactctt ctctttgaaa gaaaaaacca cccggcaagg
     5041 tggttttttt caaaggttag aaaatcctgg cagatgttct aaccgtggta acagaactat
     5101 acgagagaac tgtcaccaaa ttcgtactac cagtgtagcc gtagtcatgc ggtcacttca
     5161 agaactcctg ataattctct cgtgaattcc tgttaccaat ggctgctgcc agtggcgttt
     5221 ttgcgtgttt ttccgggttg gactcaagac gatagttacc ggacaaggcg cagcggtcgg
     5281 actgaacggg ggggttcgtg catacagtcc agcttggagc gaactgccta aacggaaccg
     5341 agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg
     5401 aaaggcggga acaggagagc gcacgaggga gccaccaggg ggaaacgcct ggtatcttta
     5461 tagtcctgtc gggtttcgcc accactgatt tgagcgtcag attctgtgat gcttgtcagg
     5521 ggggcggagc ctatggaaaa acgccccggc ggggcgtggc cttttcgccc ttgtccttag
     5581 ctttcgttat gcttaccgtt acgttatctg cttatcctcc ctccgctcgc cgcagactca
     5641 tgaccgaacg gagtgagcga atgagcgagg aagcggaaaa ccatcagaac gtacaataag
     5701 cactttcgcg ccggggcttt catgaacaaa caacaaaaga ctgccgttaa catggctaaa
     5761 ttcatccagg atcagtccct gcttcttctg gacaggctta atgagctgga tctggatcat
     5821 gaggctgatt tctgcgagaa actccatgag gatgcggaac gcctttaccg aagcctgtca
     5881 gcgcgcctga atgagcagca ggacggtgcg taatggctcc gttctgggta ttaccggagt
     5941 aataccggca tcgccttaat tttcattgtg ttagcgctta acattattac cccggtaata
     6001 cccagaatcc ctgtgttttt tattttttat ttacttttca tgaagttgcg atgaaaatcg
     6061 cagctgcgtt aggtgtatgt cattttttta gtatataaaa cgaccactcc cgagtgctgg
     6121 cgccgcaggt taagtatgat tctggctaaa aaagcgtcct tttccgggaa agggtcatgc
     6181 tcgtatgacc ctatgggttc agcgactaca cataaactac aaaattgcat ctgcttttac
     6241 cccagaacgc gggtgtacaa cagaattaca tcaaaagtac atcaaaatta catcactttt
     6301 acgtcagatc gcgggtgtac aacagaatta catcaaaagt acatcacttt tacatcattt
     6361 ttacgtcaga tctgacctct gagctattcg tacatccgcc ccctcttcct gggcctcctg
     6421 cacgtgctga aaatggtttt tccggctgtg ccggcaacgc ctcacggcca cgcaataaat
     6481 tgcgtccccg ttcaccgttt tcgggtaaga agaacatcgg aaacaggact taccccggct
     6541 gaatggcgtg aatttccgcg ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca
     6601 gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctgttttac cggtttcccg
     6661 tcagaaacgc gctcaggccg tctgagcggt gcgcgtaatg cgacgttatg gtaaatccgg
     6721 gctgttgagt gttgacctct cactatcgca acccagttaa tgcggtaaat caaacacaac
     6781 tagctgttaa ttgatgttag ttgtatttac ttgtcacttg tcttgttgac aactatcttg
     6841 tctatgttta ttctcctttt atcttaacaa gacaatggag tgtttcatga ctagtggtat
     6901 tttcttatcc cgtgaagatt tgatgttgtt atcagaccaa gcaaaagaag aagtgctggc
     6961 attggctact ggggattcgg tttctggtgt ttatgaggag tcggaagatg ggcctactga
     7021 tctttcctcg cttcaggcag aaaggcttgt tcgtggttta agtgataagt cgcgaacagt
     7081 gctgaaagct attcttgaaa gcgaggatgg tgagcgagga ttctggtgca agagcctcgc
     7141 aaaggagctt ggttttgatg tctctgagct ttcaggtgtc tggtcagggc ttacacgcag
     7201 aactcgcacg gtaagtgatg attctgaggc ttatttgatt agctgggctt gggatgatga
     7261 ccgtgatgat tactatggaa cgctacatcc aacaacctat cgcaactgca agaaagctat
     7321 gaacttttga ttgttctcgc tgtgatactt tccctgtgag cccgccatgt gcgggctttt
     7381 tttcattggt tcgtgtagtg agacgttatg gtaaatcctc gaataataaa aaaagccccc
     7441 agtggaggct tttgtgtgtg ggcactagga aaacaaacca tgctaagagg attgaattta
     7501 acgactgatt ccgctctatt tcttgtacag aaatcgccgt agcccagccg ttctggtgat
     7561 acgtaaaatc gatttgctcc aaaaatagtc ttgcatggtt ttttttagct tcctatttgg
     7621 gttgggtttc catctattgt tttgattttt ctacttttct tgttgctgtt gctacgcgct
     7681 gttgctggct tcttttcgct gtgtacaagc gttcatctaa agctaacaga tagttttcta
     7741 gatctttatt atttttaata ataattttat tcccatctaa catctctgga tagtattctt
     7801 ttatttcagc tagacattct gatatgtcgg tagtgtaaaa atcatcataa tcatcacctt
     7861 tttcaacttt tattttattt tgtttatcta attctgattg tattctcttt ttatttaata
     7921 cgtttacttt attttcatcg aaatcatcta atagatgaat gtgatcttgg ctaatgaaat
     7981 gttttgtttc ttttattcct gcttctttat atatcatttc cattccggat agaattttct
     8041 tagctttaac aatatttgaa aaataaattt ttttatcttc tatatcataa attacctctg
     8101 catattcatt tagtgtgata aatccgtctt gtaatacctc catttttttt aaagacagca
     8161 cctttgaacc aatgatattt ttttataaaa gttttggaac aaaatatatt gatcttgttt
     8221 tgtcgccatg tattttaaac catcaagatc actagcggaa aatgccttgt aattagttgt
     8281 gtttatgtca tcggattttt ttaattcctt taattcttca attatttcgt tagtgatacc
     8341 ttcgtgttta gagaggtcta aaaagaatat tttgctttga tcttgcctgt catttggatc
     8401 atactccata attccattat gttgttcttt tagttgattt ctaaagaatt tatcatctga
     8461 agatgttact ttgtagatgg ttttttttct gcttttctgc gaaaacgcaa taaggtggtt
     8521 ttttttcatt ttactctacc ttgatgaatg tgaagtcatt tattctttta tagttggagg
     8581 atgagatgtc tttatgagaa cggagatcat ccttacttat aagtaatatt tttactcctt
     8641 tatcagatgt taagtgatag aatttaaaac ctgatattaa tagtgacgga ttaaaaaaat
     8701 atacttttga gaaaaggttg aataggataa cgagaatcat aaagtagcca aaaacccagt
     8761 ttgatgtaat gctaactgag acaaaaatac cctaagtatg tcggaagaaa attatcatta
     8821 accggctcca ctgtatttat attgctcatt gactcatcgt caagtttgtt aactacgcgc
     8881 aagaatattg aattaagaaa cagaattatg agtacgctta aatgatgtag gctatgaaaa
     8941 acatagcttc attatcagag ataatagtta ctagctcttt attaaggttt gttttgtagt
     9001 ttgtgatcat gtacacaaat attgctggag ttaatgctga tattgtagat aataatttaa
     9061 agacataatc gagtatgtct gtggaattta ttttatttaa cacagtgttc tcccgtgaca
     9121 aaggcttttt tattttaaat tttgctatat gtcaataaat aaatgccggg taggtaggct
     9181 tggaaatgtt gactactgca ttagtgacag cgtactcttc ttcttgaaag aaaaaccacc
     9241 cggcaaggtg gttttttcaa aggttagaaa atcctcgcag atgttctaac cgtggtaaca
     9301 gaactatacg agagaactgt caccaaattc gtactaccag tgtagccgta gtcatgcggt
     9361 cacttcaaga actcctgata attctctcgt gaattcctgt taccaatggc tgctgccagt
     9421 ggcgtttttg cgtgtttttc cgggttggac tcaagacgat agttaccgga caaggcgcag
     9481 cggtcggact gaacgggggg ggttcgtgca tacagtccag cttggagcga actgcctaaa
     9541 cggaaccgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
     9601 gtaaaccgaa aggcgggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg
     9661 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat tctgtgatgc
     9721 ttgtcagggg ggcggagcct atggaaaaac gccccggcgg ggcgtggcct tttcgccctt
     9781 gtccttagct ttcgttatgc ttaccgttac gttatctgct tatcctccct ccgctcgccg
     9841 cagactcatg accgaacgga gtgagcgaat gagcgaggaa gcggaaaacc atcagaacgt
     9901 acaataagca ctttcgcgcc ggggctttca tgaacaaaca acaaaagact gccgttaaca
     9961 tggctaaatt catccaggat cagtccctgc ttcttctgga caggcttaat gagctggatc
    10021 tggatcatga ggctgatttc tgcgagaaac tccatgagga tgcggaacgc ctttaccgaa
    10081 gcctgtcagc gcgcctgaat gagcagcagg acggtgcgta atggctccgt tctgggtatt
    10141 accggagtaa taccggcatc gccttaattt tcattgtgtt agcgcttaac attattaccc
    10201 cggtaatacc cagaatccct gtgtttttta ttttttattt acttttcatg aagttgcgat
    10261 gaaaatcgca gctgcgttag gtgtatgtca ttttttagta tataaaacga ccactcccga
    10321 gtgctggcgc cgcaggttaa gtatgattct ggctaaaaag cgtccttttc cgggaaaggg
    10381 tcatgctcgt atgaccctat gggttcagcg actacacata aactacaaaa ttgcatctgc
    10441 ttttacccca gaacgcgggt gtacaacaga attacatcaa aagtacatca aaattacatc
    10501 acttttacgt cagatcgcgg gtgtacaaca gaattacatc aaaagtacat cacttttaca
    10561 tcatttttac gtcagatctg acctctgagc tattcgtaca tccgccccct cttcctgggc
    10621 ctcctgcacg tgctgaaaat ggtttttccg gctgtgccgg caacgcctca cggccacgca
    10681 acaaattgcg tccccgttca ccgttttcgg gtaagaagaa catcggaaac aggacttacc
    10741 ccggctgaat ggcgtgaatt tccgcgctat gcacttgtgc gcatactcat gcatgccgta
    10801 aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg ttttaccggt
    10861 ttcccgtcag aaacgcgctc aggccgtctg agcggtgcgc gtaatgcgac gttatggtaa
    10921 atccgggctg ttgagtgttg acctctcact atcgcaaccc agttaatgcg gtaaatcaaa
    10981 cacaactagc tgttaattga tgttagttgt atttacttgt cacttgtctt gttgacaact
    11041 atcttgtcta tgtttattct ccttttatct taacaagaca atggagtgtt tcatgactag
    11101 tggtattttc ttatcccgtg aagatttgat gttgttatca gaccaagcaa aagaagaagt
    11161 gctggcattg gctactgggg attcggtttc tggtgtttat gaggagtcgg aagatgggcc
    11221 tactgatctt tcctcgcttc aggcagaaag gcttgttcgt ggtttaagtg ataagtcgcg
    11281 aacagtgctg aaagctattc ttgaaagcga ggatggtgag cgaggattct ggtgcaagag
    11341 cctcgcaaag gagcttggtt ttgatgtctc tgagctttca ggtgtctggt cagggcttac
    11401 acgcagaact cgcacggtaa gtgatgattc tgaggcttat ttgattagct gggcttggga
    11461 tgatgaccgt gatgattact atggaacgct acatccaaca acctatcgca actgcaagaa
    11521 agctatgaac ttttgattgt tctcgctgtg atactttccc tgtgagcccg ccatgtgcgg
    11581 gcttttttca ttggttcgtg tagtgagacg ttatggtaaa tcctcgaata ataaaaaagc
    11641 ccccagtgga ggcttttgtg tgtgggcact aggaaaacaa accatgctaa gaggattgaa
    11701 tttaacgact gattccgctc tatttcttgt acagaaatcg ccgtagccca gccgttctgg
    11761 tgatacgtaa aatcgatttg ctccaaaaat agtcttgcat ggtttttttt agcttcctat
    11821 ttgggttggg tttccatcta ttgttttgat ttttctactt ttcttgttgc tgttgctacg
    11881 cgctgttgct ggcttctttt cgctgtgtac aagcgttcat ctaaagctaa cagatagttt
    11941 tctagatctt tattattttt aataataatt ttattcccat ctaacatctc tggatagtat
    12001 tcttttattt cagctagaca ttctgatatg tcggtagtgt aaaaatcatc ataatcatca
    12061 cctttttcaa cttttatttt attttgttta tctaattctg attgtattct ctttttattt
    12121 aatacgttta ctttattttc atcgaaatca tctaatagat gaatgtgatc ttggctaatg
    12181 aaatgttttg tttcttttat tcctgcttct ttatatatca tttccattcc ggatagaatt
    12241 ttcttagctt taacaatatt tgaaaaataa atttttttat cttctatatc ataaattacc
    12301 tctgcatatt catttagtgt gataaatccg tcttgtaata cctccatttt ttttaaagac
    12361 agcacctttg aaccaatgat atttttttat aaaagttttg gaacaaaata tattgatctt
    12421 gttttgtcgc catgtatttt aaaccatcaa gatcactagc ggaaaatgcc ttgtaattag
    12481 ttgtgtttat gtcatcggat ttttttaatt cctttaattc ttcaattatt tcgttagtga
    12541 taccttcgtg tttagagagg tctaaaagaa tattttgctt tgatcttgcc tgtcatttgg
    12601 atcatactcc ataattccat tatgttgttc ttttagttga tttctaaaga atttatcatc
    12661 tgaagatgtt actttgtaga tggttttttt tctgcttttc tgcgaaaacg caataaggtg
    12721 gttttttttc attttactct accttgatga atgtgaagtc atttattctt ttatagttgg
    12781 aggatgagat gtctttatga gaacggagat catccttact tataagtaat atttttactc
    12841 ctttatcaga tgttaagtga tagaatttaa aacctgatat taatagtgac ggattaaaaa
    12901 aatatacttt tgagaaaagg ttgaatagga taacgagaat cataaagtag ccaaaaaccc
    12961 agtttgatgt aatgctaact gagacaaaaa aataccctaa gtatgtcgga agaaaattat
    13021 cattaaccgg ctccactgta tttatattgc tcattgactc atcgtcaagt ttgttaacta
    13081 cgcgcaagaa tattgaatta agaaacagaa ttatgagtac gcttaaaatg atgtaggcta
    13141 tgaaaaacat agcttcatta tcagagataa tagttactag ctctttatta aggtttgttt
    13201 tgtagtttgt gatcatgtac acaaatattg ctggagttaa tgctgatatt gtagataata
    13261 atttaaagac ataatcgagt atgtctgtgg aatttatttt atttaacaca gtgttctccc
    13321 gtgacaaagg ctttttattt taaattttgc tatatgtcaa taaataaatg ccgggtaggt
    13381 aggcttggaa atgttgacta ctgcattagt gacagcgtac tcttctcttt gaaagaaaaa
    13441 accacccggc aaggtggttt tttcaaaggt tagaaaatcc tggcagatgt tctaaccgtg
    13501 gtaacagaac tatacgagag aactgtcacc aaattcgtac taccagtgta gccgtagtca
    13561 tgcggtcact tcaagaactc ctgataattc tctcgtgaat tcctgttacc aatggctgct
    13621 gccagtggcg tttttgcgtg ttttccgggt tggactcaag acgatagtta ccggacaagg
    13681 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
    13741 aaacggaacc gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca
    13801 ccggtaaacc gaaaggcggg aacaggagag cgcacgaggg agccaccggg ggaaacgcac
    13861 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag attctgtgat
    13921 gcttgtcagg gggcggagcc tatggaaaac gccccggcgg ggcgtggcct tttcgccctt
    13981 gtccttagct ttcgttatgc ttaccgttac gttatctgct tatcctccct ccgctcgccg
    14041 cagactcatg accgaacgga gtgagcagat gagcgaggaa gcggaaaacc atcagaacgt
    14101 acaataagca ctttcgcgcc ggggctttca tgaacaaaca acaaaagact gccgttaaca
    14161 tggctaaatt catcggggat cagtccctgc ttcttctgga caggcttaat gagctggatc
    14221 tggatcatga ggctgatttc tgcgagaaac tccatgagga tgcggaacgc ctttaccgaa
    14281 gcctgtcagc gcgcctgaat gagcagcagg acggtgcgta atggctccgt tctgggtatt
    14341 accggagtaa taccggcatc gccttaattt tcattgtgtt agcgcttaac attattaccc
    14401 cggtaatacc cagaatccct gtgtttttat tttttattta cttttcatga agttgcgatg
    14461 aaaatcgcag ctgcgttagg tgtatgtcat tttttagtat ataaaacgac cactcccgag
    14521 tgctggcgcc gcaggttaag tatgattctg gctaaaaagc gtccttttcc gggaaagggt
    14581 catgctcgta tgaccctatg ggttcagcga ctacacataa actacaaaat tgcatctgct
    14641 tttaccccag aacgcgggtg tacaacagaa ttacatcaaa agtacatcaa aattacatca
    14701 cttttacgtc agatcgcggg tgtacaacag aattacatca aaaagtacat cacttttaca
    14761 tcatttttac gtcagatctg acctctgagc tattcgtaca tccgccccct cttctgggcc
    14821 tcctgcacgt gctgaaaatg gttttccggc tgtgccggct aacgcctcac ggccacgcaa
    14881 taaattgcgt ccccgttcac cgttttcggg taagaagaac atcggaaaca ggacttaccc
    14941 cggctgaatg gcgtgaattt ccgcgctatg cacttgtgcg catactcatg catgccgtaa
    15001 aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt tttaccggtt
    15061 tcccgtcaga aacgcgctca ggccgtctga gcggtgcgcg taatgcgacg ttatggtaaa
    15121 tccgggctgt tgagtgttga cctctcacta tcgcaaccca gttaatgcgg taaatcaaac
    15181 acaactagct gttaattgat gttagttgta tttacttgtc acttgtcttg ttgacaacta
    15241 tcttgtctat gtttattctc cttttatctt aacaagacaa tggagtgttt catgactagt
    15301 ggtattttct tatcccgtga agatttgatg ttgttatcag accaagcaaa agaagaagtg
    15361 ctggcattgg ctactgggga ttcggtttct ggtgtttatg aggagtcgga agatgggcct
    15421 actgatcttt cctcgcttca ggcagaaagg cttgttcgtg gtttaagtga taagtcgcga
    15481 acagtgctga aagctattct tgaaagcgag gatggtgagc gaggattctg gtgcaagagc
    15541 ctcgcaaagg agcttggttt tgatgtctct gagctttcag gtgtctggtc agggcttaca
    15601 cgcagaactc gcacggtaag tgatgattct gaggcttatt tgattagctg ggcttgggat
    15661 gatgaccgtg atgattacta tggaacgcta catccaacaa cctatcgcaa ctgcaagaaa
    15721 gctatgaact tttgattgtt ctcgctgtga tactttccct gtgagcccgc catgtgcggg
    15781 cttttcattg gttcgtgtag tgagacgtta tggtaaatcc tcgaataata aaaaagcccc
    15841 cagtggaggc ttttgtgtgt gggcactagg aaaacaaacc atgctaagag gattgaattt
    15901 aacgactgat tccgctctat ttcttgtaca gaaatcgccg tagcccagcc gttctggtga
    15961 tacgtaaaat cgatttgctc aaaaatagtc ttgcatggtt ttttagcttc ctatttgggt
    16021 tgggtttcca tctattgttt tgattttcta cttttcttgt tgctgttgct acgcgctgtt
    16081 gctggcttct tttcgctgtg tacaagcgtt catctaaagc taacagatag ttttctagat
    16141 ctttattatt tttaataata attttattcc catctaacat ctctggatag tattctttta
    16201 tttcagctag acattctgat atgtcggtag tgtaaaaatc atcataatca tcaccttttt
    16261 caacttttat tttattttgt ttatctaatt ctgattgtat tctcttttta tttaatacgt
    16321 ttactttatt ttcatcgaaa tcatctaata gatgaatgtg atcttggcta atgaaatgtt
    16381 ttgtttcttt tattcctgct tctttatata tcatttccat tccggataga attttcttag
    16441 ctttaacaat atttgaaaaa taaatttttt tatcttctat atcataaatt acctctgcat
    16501 attcatttag tgtgataaat ccgtcttgta atacctccat tttttttaaa gacagcacct
    16561 ttgaaccaat gatatttttt tataaaagtt ttggaacaaa atatattgat cttgttttgt
    16621 cgccatgtat tttaaaccat caagatcact agcggaaaat gccttgtaat tagttgtgtt
    16681 tatgtcatcg gattttttaa ttcctttaat tcttcaatta tttcgttagt gataccttcg
    16741 tgtttagaga ggtctaaaaa gaatattttg ctttgatctt gcctgtcatt tggatcatac
    16801 tccataattc cattatgttg ttcttttagt tgatttc
//
