LOCUS       NZ_CP137750            13353 bp    DNA     circular CON 09-NOV-2023
DEFINITION  Acinetobacter sp. 16 plasmid pKO16_YMB, complete sequence.
ACCESSION   NZ_CP137750
VERSION     NZ_CP137750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37871729
            Assembly: GCF_033439925.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. 16
  ORGANISM  Acinetobacter sp. 16
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Ko,S.Y., Rana,M.S., Kim,S., Kim,S.Y., Kim,N., Lee,D.E., Hwang,H.R.,
            Kim,Y.K., Kwon,K.T. and Lee,J.C.
  TITLE     Genomic study of Acinetobacter junii strain 16
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13353)
  AUTHORS   Ko,S.Y., Rana,M.S., Kim,S., Kim,S.Y., Kim,N., Lee,D.E., Hwang,H.R.,
            Kim,Y.K., Kwon,K.T. and Lee,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Department of Microbiology, Kyungpook
            National University School of Medicine, Jung-gu, Daegu 41944, South
            Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. December-2021
            Assembly Name                     :: De novo
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 710.9x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/09/2023 04:21:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,570
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,291
            CDSs (with protein)               :: 3,291
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 76 of 181
            Pseudo Genes (incomplete)         :: 103 of 181
            Pseudo Genes (internal stop)      :: 40 of 181
            Pseudo Genes (multiple problems)  :: 31 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137750.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13353
                     /organism="Acinetobacter sp. 16"
                     /mol_type="genomic DNA"
                     /strain="16"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:3081771"
                     /plasmid="pKO16_YMB"
                     /geo_loc_name="South Korea: Daegu"
                     /collection_date="2015-12-08"
                     /collected_by="Md Shohel Rana"
                     /identified_by="Md Shohel Rana"
     gene            complement(join(12976..13353,1..9))
                     /locus_tag="R2H34_RS17760"
     CDS             complement(join(12976..13353,1..9))
                     /locus_tag="R2H34_RS17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769969.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_002125026.1"
                     /translation="MSLISSTFVIAVHDEILLQTGVGRSGCQIEKLESVLNRIDQQMYY
                     SNVSDIFEIAAWFGIAISKGHAFVDGNKRTGLAVMLTYLEIQGVTIQEHTGLDDLMVDI
                     VESQEDHELLAKLVAGFLYELSII"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(6..185)
                     /locus_tag="R2H34_RS17765"
     CDS             complement(6..185)
                     /locus_tag="R2H34_RS17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_026441249.1"
                     /translation="MRKRLKDYNIEQVSSREKVSGQVLPIKFSNDEATKRVVMHSARRV
                     IQQHKAEIQELAYK"
                     /name="hypothetical protein"
     gene            complement(255..590)
                     /locus_tag="R2H34_RS17770"
     CDS             complement(255..590)
                     /locus_tag="R2H34_RS17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002125021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002125021.1"
                     /translation="MKIGIFWYWNNQVIGIAHSFNITKADSIGLIDSPYTHIEYWITLQ
                     EKYSELQNYEYEQIPRGRVIFDTNKEKSIIYLDKTLLYKSKINKVYDFFNLNPEQSVLK
                     KDSHYQI"
                     /name="hypothetical protein"
     gene            complement(1111..1746)
                     /locus_tag="R2H34_RS17775"
     CDS             complement(1111..1746)
                     /locus_tag="R2H34_RS17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="WP_151708324.1"
                     /translation="MDYLAQFTTYIFTLSLAAILPGPGMTGLMFKTLTQGYKNGLIMLI
                     GLITGDLIFLLTSIFLLTSLTQFFPNFSFYMVLLSSIYLLYLAYSFWSFNGDLLQTQAP
                     NTVNETIFSYRDGLLITLSNPKTISFYLAIVPAIFGAYSLKDQTFALMIITVLTLTVIG
                     GFYVFFSLGLKKFLRNLKIQRTLLKGLSLMMFILALSMIYREFTPHLT"
                     /name="LysE family translocator"
     gene            1877..2359
                     /locus_tag="R2H34_RS17780"
     CDS             1877..2359
                     /locus_tag="R2H34_RS17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080650.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_151708325.1"
                     /translation="MPNHKKIDKTDVKILELLQQDSRITTAQLSEELSLSTSPCWRRVK
                     QLEDTKIIKGYGVLLDRKKINLGVMVFIRVSIDSHSEKEAKKFEEEVTALDYVVACYSI
                     GGDADFLLQVVSPDLDTYAEFSMSIIRRLPGIKEMQSMFVLKEIKPFIGFPVKPSQ"
                     /name="Lrp/AsnC family transcriptional regulator"
     gene            complement(2558..2857)
                     /locus_tag="R2H34_RS17785"
     CDS             complement(2558..2857)
                     /locus_tag="R2H34_RS17785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_318342612.1"
                     /translation="MLQHEFDQETIDHCQADIQAGIERIIRGESTQKEEFSKMLDDMAQ
                     EIGVLGTPNSDISAVNAEVIRLQDEAAQKPDHWLHDGLKSHFEEIQQQLSDLKC"
                     /name="hypothetical protein"
     gene            complement(3015..3350)
                     /locus_tag="R2H34_RS17790"
     CDS             complement(3015..3350)
                     /locus_tag="R2H34_RS17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032072989.1"
                     /translation="MALKGLKKENLAPKEEVAINDFISGATKQVQSLTPSNAKFQRYTF
                     SLTDEVSEQIDGIKVRCRVAKANRSIILKAAIHQLSKLSDEELKALILSELGMNAIPRE
                     QGKDKKR"
                     /name="hypothetical protein"
     gene            complement(3350..3991)
                     /locus_tag="R2H34_RS17795"
     CDS             complement(3350..3991)
                     /locus_tag="R2H34_RS17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_032072990.1"
                     /translation="MIILVGGEKGGSGKSCLAQNLAVYLQANHRDILLLDADPQGTTSD
                     WIKERDENEKVQNIPSVQASGNIRQILIDLSKRYQDIIIDAGGQDSEALRSAMTISTHM
                     LLPFRPKRRDLKTLAHVEKLIRLAKAVNPELYTRAVITQCPTLPSQMQRILDAKEACES
                     FDLTPLNNYTCNRNIYDDADENGLSVFEMSSDEKAKQEIEEIAKEFLGEL"
                     /name="AAA family ATPase"
     gene            4336..5265
                     /locus_tag="R2H34_RS17800"
     CDS             4336..5265
                     /locus_tag="R2H34_RS17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_118901254.1"
                     /translation="MEQLSFLLDQFQDRLPRKPYCTNDLDFGLIIRPKEVSLKYKYIQP
                     NSPYYLHYLIFDLDYDASLSEILYELVGIPLPNLLVANPENGRSHILFQLKVPIYTTDA
                     SRQKPILYANAIQQKLLELFKADPAYVGLVAKNPFSDSWKTYCLRDKPYSLNELAKNLE
                     LSWKDANKEIKQDDAIGLGRNCFVFHTARHWAYKEVRQYRGSTYTAWLDCVVKHCSGLN
                     QGLNQPMTHGEVKGIAKSIARYCWKKDAYCYQEFIDRQSRKGTVGGKKSKRGCKDDSER
                     SMKPWEELGIGQATYYRRKKKGLLSDSI"
                     /name="replication initiation protein"
     gene            complement(5419..5682)
                     /locus_tag="R2H34_RS17805"
     CDS             complement(5419..5682)
                     /locus_tag="R2H34_RS17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032072983.1"
                     /translation="MTIQKRLNKDSVQLIKIAHRVEFVRLQHESGSQKVIAQIYIAHDA
                     YPIRAMAGDLTWDAHDIEKSKRSIRRHNKTCLIIDRRDQIVS"
                     /name="hypothetical protein"
     gene            complement(5696..6259)
                     /locus_tag="R2H34_RS17810"
     CDS             complement(5696..6259)
                     /locus_tag="R2H34_RS17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641851.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_032072984.1"
                     /translation="MANVGYIRVSSADQNTARQLDGVQLDKVFTEKVSGATADREQLHA
                     MLDYVREGDTVHVHEISRLARSLIDLNTLISDLNKKGVTVVFHQERMTFSPDREQEPLQ
                     QLMFNMLASFAQFERQIIKQRQAEGIAKAKERGEYMGRKKTIDDSAIIEAMSKDGASFR
                     KVAKELSVSLSTVQRAMKKHSPAC"
                     /name="recombinase family protein"
     gene            complement(6757..7326)
                     /locus_tag="R2H34_RS17815"
     CDS             complement(6757..7326)
                     /locus_tag="R2H34_RS17815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_318342609.1"
                     /translation="MNELYESCASFQNNVIEKINAEVGQINEFFDQKISDISKAVQNSC
                     SNTLQTMSDTEQQINQKSKQLTSVLEKNTNASIAMTEKIIEKQENIYKNLCKKLNFKYF
                     SFNSISILIVTLVILCGLNIAATMRYEKMKNYNIALVSQNQKLQSDIKDLEATRHDLIV
                     LTKQSVNEVRKKFPQLQIGINCKSLD"
                     /name="hypothetical protein"
     gene            7526..8494
                     /locus_tag="R2H34_RS17820"
     CDS             7526..8494
                     /locus_tag="R2H34_RS17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_014538385.1"
                     /translation="MLESRKEGFSARKFAELIKRHPSTIYRELKRNSINDVYQARYASD
                     NTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTFPDLDQTMNVSHTTIYS
                     TIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKTIHERPAEVQERKIPGHW
                     EADLIKGKDNKSSIATLIERNTRLCILATLPDAKAESVRKALTEALKYLPAELRKTLTY
                     DRGREMAEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYL
                     NQVAMSLNTRPRKALDWLTPLEKFAQLVDYHKTFQTVAPHV"
                     /name="IS30 family transposase"
     gene            complement(8429..9091)
                     /locus_tag="R2H34_RS17825"
     CDS             complement(8429..9091)
                     /locus_tag="R2H34_RS17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_318342610.1"
                     /translation="MSDQQIAATLKAMSDALHKLSMSLESEKQEHIELLKKLNEHLTHS
                     QDAIEDNIFDFLKRIELTELLNTVNESKNNIQSQKENIDKLNEEIESIDKNIKNSLNVL
                     DEQTEYISKKIQNNSNLIQTEIVKNVSVSVKESITTQFLNQFSEQNQNLVTKIIEANEK
                     AVNVSVSIYKALIIEVEKIKSNHIESLENFKEHVANSNMRCDSLKSLMIINKLSKFL"
                     /name="hypothetical protein"
     gene            complement(9101..10315)
                     /gene="mobQ"
                     /locus_tag="R2H34_RS17830"
     CDS             complement(9101..10315)
                     /gene="mobQ"
                     /locus_tag="R2H34_RS17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_318342611.1"
                     /translation="MAIYHFSVKTISRSDGRSAVAAAAYRAAEKLTCDYYGKEQDYTKK
                     SGVEHTQIYAPENTQEELLDRQTLWNTVEKSERRKDALLGREFEIAFPSELNAQQRQAM
                     LDELCHDLVKKHGVIVDAAIHAPHIDGGSDERNYHAHIMFTTRAIDPENGEFSAKKYRD
                     FSRDHGTETVSRWRKDFADLANRHLEQAGVDVRIDHRSYADQNHGLQATSHEGPSVTAL
                     RRQYEREQQKPFNERNHEIVLPALAKQNDAIKLFNLELEITATEKLIKIMQHEAKKTFE
                     SILSEEKTTNFENFDQILQKSHETRSEKNIFDTLNSSISRLRKAGLFSQLDASINNQAE
                     AESQARLQAESRAKLEAEHIAKEEQRLKAQAEAKTKLEAERQAKIRAEAESLAQARRND
                     FNPLD"
                     /name="mobQ"
     gene            10547..10807
                     /locus_tag="R2H34_RS17835"
     CDS             10547..10807
                     /locus_tag="R2H34_RS17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005000600.1"
                     /translation="MSLDYLNEKIAKQEERLKQLKVQKQATIAREKAKQKEQDRKDDTR
                     RKILLGSYLLKKMENEENKEKILAEMNEYLTQERDRKLFNL"
                     /name="hypothetical protein"
     gene            11148..11495
                     /locus_tag="R2H34_RS17840"
     CDS             11148..11495
                     /locus_tag="R2H34_RS17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004641894.1"
                     /translation="MQIKFNNANPCMPKNLAKQLIVESAKLLNHQQRQALLDIFMTLDQ
                     AERTAENTLQQHAVAQQSTHNFSQDYECQEYNQEYWSGHYYDSYSQEYPEDWVKRTQST
                     PGRHKFPRTAY"
                     /name="hypothetical protein"
     gene            complement(11845..12360)
                     /locus_tag="R2H34_RS17845"
     CDS             complement(11845..12360)
                     /locus_tag="R2H34_RS17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002125020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002125020.1"
                     /translation="MTEKEHINQYIKSTCDLIIQHVKNIITLQENINKPWGYSSRLMEH
                     LFHPENELLFKGYSKNIFNNKSAMAIKPWKEHIVPMAYVFNNLWVLIESNELSDAELSK
                     ILQRNLGVAHISYEEQKKLDTKFSGLKTNMPNGWCLKTGDPIDRLKAVGIELVDENGVE
                     IQSLITPI"
                     /name="hypothetical protein"
     gene            12414..12974
                     /locus_tag="R2H34_RS17850"
     CDS             12414..12974
                     /locus_tag="R2H34_RS17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002125033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WP_118901251.1"
                     /translation="MSNLKKVTLIQTDITTFAVQAIVNSANKSLLGGSGLDYIIHKKAG
                     PLMKTECIQLHQEKGGCSTGQAEVTTAGNLPAQYLIHAVGPRWLDGERNEPQLLCDAYS
                     NAIMKANHVGVKTISFPNISTGVYGFPRQLAAEIAIGTILSTLPQYDCVEEVFFVCRDD
                     ENYLIYKEILSNINDPKIKIQIE"
                     /name="macro domain-containing protein"
     Typing          4526..5265
                     /name="rep_cluster_1740"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9105..10315)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.752"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caagctcatt tataagctaa ctcttgtatt tctgctttat gttgctggat cactcgtcta
       61 gcactatgca taacaactcg tttagttgct tcatcattac taaactttat aggtaaaact
      121 tgtccagaaa ccttttcacg actagatact tgttcaatat tataatcttt aagacgtttt
      181 ctcatatcgc ttaccattct aagagtgatc gtgtgtattt taacgcaagt ttgttgatct
      241 aacagtagtg ttggtcaaat ttgatagtgt gagtcttttt ttagcaccga ttgttctgga
      301 tttaaattaa aaaaatcata gactttatta atttttgatt tatataacaa tgtcttatcc
      361 aaataaataa ttgatttttc tttgttggta tcaaaaatta ctcgacctct tgggatctgt
      421 tcatactcat agttttgtag ttcagaatac ttttcttgca atgtaatcca atattcaata
      481 tgtgtatatg ggctatcaat caggccaata gagtctgctt tcgtaatatt aaaggaatgt
      541 gcaataccaa tgacttgatt attccagtac caaaagatcc ctattttcat ttgtctttat
      601 gcctagtttt aagttcaact aaaattaata taatacggat tatccgaaaa tattcagaca
      661 ataaaaaacc ccgacatcct gtcggggttt ttcgtatttg gtactagggt caactcctgt
      721 catccctcgc gtcctgtcgc tgatcttatt gttgttatgt ggaacatcct gttctcgatg
      781 aactcatcat aggaaattga gctgagtatg gctattggca attttcttga atggctgtac
      841 gccaaaacgt acatgatttt atcagggatt tatcccgata tttgtccgca ggaaatgggg
      901 ataatttcag aaatgaaggc ctttagaaat caggaaaatt tggatttttg aatatttttt
      961 aaccaatttt attggctaaa agtgacaaaa gtgcttaaaa agcagccgac ttgtttcgga
     1021 gtcggctttt tagctatttt ttagtcatga ctaatttttt aaatatttga aaaacaataa
     1081 ctttttataa atgatttcgc ataacgccca ttatgttaaa tgaggcgtaa attctctata
     1141 tatcatgctt agagctaaga taaacatcat aagagatagt cccttaagca aagttctttg
     1201 aattttcaaa ttacgtaaaa acttttttag gcctaaagaa aaaaatacat aaaaaccgcc
     1261 aataacagtt aaagtcagaa ctgtaattat cattaaggca aaagtttgat ctttcagact
     1321 atatgctcca aaaatagctg gaacaattgc taggtaaaac gaaatggttt taggattact
     1381 gagtgttatc aatagcccat ctctataaga gaatatagtt tcatttaccg tattaggagc
     1441 ctgagtttgt agcagatcac cattaaaaga ccaaaaacta taagctaaat acaataaata
     1501 aatactcgat agtaggacca tataaaaaga aaaatttgga aaaaattgag ttaaagaagt
     1561 cagtaaaaaa atagaggtta atagaaagat tagatcacct gtgattagac caattagcat
     1621 aatcaagcca tttttatagc cctgtgtcag tgttttaaac attagacctg tcataccagg
     1681 cccaggaaga attgcggcta aagataaagt gaaaatatat gtggtgaatt gtgctagata
     1741 gtccatttga aggtaaatca ataaaagaat tgagactatt ttttagtaaa aacaaagaag
     1801 aaattgatct atatttttta taaaattgat aaataagggt ataaaatttc tattttattt
     1861 aataatttta gtatttatgc ctaatcacaa aaaaattgat aaaaccgatg taaaaatttt
     1921 ggagttgttg caacaggact cacgtattac aacagcccag ttatcagaag aattgagctt
     1981 atcaacttct ccatgttggc gtagagtcaa gcaacttgaa gatactaaga ttattaaagg
     2041 atatggagta ttacttgatc gtaaaaaaat aaatttaggt gtaatggtat ttatacgtgt
     2101 atctattgat agccatagtg aaaaagaagc taaaaaattt gaggaagaag tgacggcttt
     2161 agattatgtc gttgcatgtt acagcattgg aggtgatgca gatttcttac ttcaagttgt
     2221 atccccagat cttgatactt atgctgaatt ctctatgtca ataataagaa gattgccggg
     2281 aataaaagaa atgcagagta tgtttgtttt aaaagaaata aaaccattca taggttttcc
     2341 agtgaagcct tctcaatagt attctaagtt aagttttcct aaaattaaca taatacacct
     2401 tatgcgaaat tcaatatttt atcttataaa aatcattgct ttatctgata ttgaaaagcc
     2461 caatcggtaa gggttgggct tttttattga tttttcacgt tccacgctta atatttgatc
     2521 gttgtttcac ggttttgatt agtttgatta cataatccta gcatttaagg tctgacaatt
     2581 gctgctgaat ttcttcaaaa tgtgatttta agccgtcatg cagccaatga tcaggcttct
     2641 gagctgcttc atcttgcagg cggatcactt cggcattgac tgctgaaatg tcgctgtttg
     2701 gagtgcctag cactcctatt tcctgtgcca tatcatctag cattttggaa aactcctcct
     2761 tttgagtgga ttcaccacgg ataatacgct caatccctgc ctgtatgtcc gcttggcagt
     2821 ggtcaattgt ttcctgatcg aattcatgtt gcagcacacc tgctgaccat cggtaagatg
     2881 ctcttaacgc ctctgcttcc gcttcggtgt atatgcgcct attttaattt tgtcatcggg
     2941 atagtctctc agaatattga ctctccgaca aacccggtac ggttcaatgc gattcatgta
     3001 gggatttacg gttgttatct tttcttatct tttccttgct ctctaggaat agcattcatg
     3061 cctaattcac ttaagatcag agctttaagt tcttcatcgg ataatttact taattgatgt
     3121 attgcagctt tgagaataat ggagcgattc gctttagcaa cacggcatct tacctttata
     3181 ccgtcaatct gctcactcac ctcgtcagtt aaagaaaaag tgtatcgctg aaatttagca
     3241 ttagacggag ttaaactttg gacttgctta gttgcacctg aaataaaatc gttaattgca
     3301 acttcttctt taggagctag attttccttt ttcagacctt ttagagccat taaagttctc
     3361 ctaaaaattc tttggcaatc tcttcaattt cttgcttggc tttttcatca gatgacattt
     3421 caaaaacaga taagccgttt tcatccgcat catcgtaaat atttcgatta caggtgtagt
     3481 tatttaaagg tgttagatcg aaagattcac aggcttcttt agcgtcaaga atgcgttgca
     3541 tttggcttgg taaagttggg cactgcgtta tcactgctct cgtataaagt tcaggattca
     3601 ccgccttagc taaacgaatg agtttctcga catgagccag tgtttttaga tcacgtcttt
     3661 tgggtctaaa tggcaacagc atgtgtgttg atatggtcat ggctgaacgc aatgcttcgg
     3721 aatcttgtcc gcctgcatca atgatgatgt cttgatagcg tttagataaa tctatgagga
     3781 tttggcgaat attccccgat gcttgaacag atggaatgtt ttgtactttc tcattttcat
     3841 cacgttcttt tatccagtcg cttgttgttc cttgagggtc ggcatcgagg agcaaaatat
     3901 cccgatgatt cgcttgcaag taaactgcta gattttgagc taaacaactt ttacccgaac
     3961 cgcccttttc accaccaacc aatatgatca tttttattaa acttacgtaa cttatttaag
     4021 ttaagtatag gctaaaatta tagaagatca acagattcac caacttatac aaaatacata
     4081 acttacgtaa gtttaataac ttacataagt taatggaaaa acttttattt tttatatgtt
     4141 tttcatgttg atttggtgcg agagttctat atattgtatt tattacacca ataaaaaagc
     4201 cccataactt tctaatcttg gcggactgga agttacaggg cttgaattct taaatcatac
     4261 aaaccgtaaa gttcgtataa cttgaaatca ataatacagg ttatgcgtgc atttgcaaat
     4321 agtgaagact agcaaatgga gcaactatct tttctgcttg atcagtttca agacagattg
     4381 ccaaggaagc cgtattgtac gaatgacttg gattttggtc ttatcattag acccaaagag
     4441 gtatccttaa aatataagta tatccagccg aatagtccct attatttaca ctatctaatc
     4501 tttgacctag attacgatgc gtcattgtct gaaatcttat atgaacttgt cggtatacct
     4561 ttacccaact tgttagtcgc caatcctgaa aatggacgct cacatatctt atttcagcta
     4621 aaagttccta tctatacaac ggatgctagc cgacaaaaac caattcttta cgccaatgct
     4681 attcagcaaa aactactgga gttattcaaa gcagacccag cgtatgtagg cttggtggca
     4741 aagaatcctt tttctgattc atggaaaact tattgtctgc gtgataaacc gtattcgctg
     4801 aatgaattag ccaaaaattt agagctaagc tggaaagatg cgaataaaga aatcaaacag
     4861 gatgatgcta ttggcttagg gcgcaactgt tttgtgtttc atacagctag gcactgggcg
     4921 tataaggaag ttaggcaata cagaggcagc acctatacag cgtggctaga ttgcgttgtt
     4981 aagcattgtt caggcttaaa ccaagggctt aatcaaccta tgacgcatgg cgaagtgaaa
     5041 ggcattgcta agtcgattgc tcgttattgt tggaagaaag atgcttattg ctatcaggaa
     5101 ttcattgatc ggcagagccg taagggcact gttggtggta agaagtctaa acgtggctgt
     5161 aaggacgatt cagagcgttc tatgaagcct tgggaagaat taggaatagg gcaagcaaca
     5221 tactacagaa gaaagaaaaa aggattgtta agtgatagca tttaagccta aatcagatat
     5281 tagctttttt tgcctctttc gttggtcgtt ttggtcgtgg gcttagagcg gttttgaagt
     5341 aattaagggc gactaattgt gtgcttatgc caatcattag attaaccctc actctaatct
     5401 gagggcttta ttgaatgttt aacttacaat ctgatcacgt ctatcaataa ttaaacaagt
     5461 cttgttgtgt ctacgaatag aacgcttaga tttttctatg tcgtgagcgt cccatgttaa
     5521 gtcgccagcc attgcacgaa tcgggtaagc atcatgtgca atgtagatct gtgcaataac
     5581 tttctgagag ccgctctcat gttgtagtct aacgaactca acacggtgtg caattttaat
     5641 caattgcaca gaatctttat ttaagcgttt ttgaatagtc atgattagcc cccgattaac
     5701 acgctgggct atgtttcttc attgctcttt gaactgtgga caatgacaca ctcagctctt
     5761 tagcaacttt tctaaatgac gcgccgtctt tgctcattgc ttcaatgatt gcagaatcat
     5821 caattgtctt tttgcgtccc atgtattcac ctctttcttt agccttagca atgccctcag
     5881 cttgtctttg cttaatgatc tggcgttcaa actgtgcaaa tgatgcaagc atgttaaaca
     5941 tgagctgttg caacggttct tgttcacggt ctgggctaaa cgtcatgcgt tcttgatgaa
     6001 aaacaaccgt aacgcctttt ttgtttagat cgctgatcaa tgtatttaga tcaatcaagc
     6061 ttcgcgctag tcttgagatt tcatgcacat gaacggtgtc accctcacgt acataatcaa
     6121 gcattgcatg aagctgctca cgatccgcag tcgcaccgct tactttttca gtgaacactt
     6181 tatcaagctg cacaccgtcc aattgacgcg ctgtattttg atctgcactg ctgacacgta
     6241 tataaccgac attcgccatg ttgtccccta ttgtattctt ttgactctaa gcaattaaga
     6301 atatagtgta ttcataatcc aaaatcaagt tttatgaata cggtttttag tattaaataa
     6361 gtgtattcct tagcttgtac tggactgaac acctgatgga ttcccataga ctctgataag
     6421 ttcagataaa tctataagac catcatgatc ccttaataat tcaccatttt ttatagcgtc
     6481 atatattgtt gtacgtgatt ttttaaatgc tttactcgcg tccataacgc taaattttgt
     6541 tgtgttcaat ggaatccctc agtgcgtgta caaggatgtt caagggagct tgtaatcagg
     6601 gaaagagcag ggcgaaattt ttatatcgct cactcgctct gcatatgctg tttaaatgca
     6661 aaaaaaaggg gtttctggat cgatttaagc tattttcata gaacctataa agatttatca
     6721 aaagtcgctt aaatcgcaaa tgagagataa ataggcttag tctaatgatt tacagtttat
     6781 tcctatctga agctgaggaa atttctttct cacttcatta actgattgct ttgttaaaac
     6841 aattaaatca tgtctggttg cttccagatc tttgatgtca gattgcagtt tttgattttg
     6901 gctgactaat gcgatgttgt aatttttcat tttttcatat cgcatcgttg cagcgatgtt
     6961 gagaccgcag agaataacaa gagtaacaat aagaatagaa attgaattaa atgaaaaata
     7021 cttgaaattc aattttttac atagattttt atagatattt tcttgttttt ctataatttt
     7081 ttcggtcatt gcaatgcttg cattagtgtt tttttctaaa acagacgtca gttgcttgga
     7141 tttctgattg atctgctgtt ctgtgtcgct cattgtttgc agagtattgc tgcagctatt
     7201 ttgtactgct ttagaaatat cactaatttt ttgatcaaaa aattcattga tttgtccaac
     7261 ctctgcattt attttttcaa tgacattatt ttgaaagctg gcgcaagatt cataaagctc
     7321 attcatactg ttaatattat gttctttgac ttctgaaaat gcacttccga tattttttat
     7381 tgcagaattg atttctgcat tgaatgcatt aacgggcaga gtaaaacttg aagtgcgaca
     7441 taaaccacct aattaattta aagggtttat ggagtatata aaattgtcat accatcatct
     7501 taactttgaa gatcgtactg cattaatgct tgagtcaaga aaagaaggct tttcagccag
     7561 aaaatttgct gaacttatta aaagacatcc tagtacgatc tatcgtgagc ttaaaagaaa
     7621 tagcatcaat gacgtttatc aagctcgata tgcttctgat aacacttttg ctagacgtag
     7681 acgtggtcac agaaaactca aaatcgattc aatcctctgg aaatttattg ttgaagcgat
     7741 ccgttgttta tggtctcctc agcaaatagc aaagcgttta aagacatttc ctgatttgga
     7801 tcaaacaatg aatgtaagcc atacaacgat ttattcaacg atacgagcat tacccaaggg
     7861 tgagttgaaa aaagacttat tatcctgtct acgtcatgaa aataaaaagc gaaaagctaa
     7921 cggtgaacct aaaaaagatt ctatattaca ggatattaaa actattcatg agcgcccagc
     7981 cgaagttcaa gaaagaaaaa taccgggtca ttgggaagct gatttaatta aaggtaaaga
     8041 caataaaagt tcgatagcaa cacttattga acgaaataca cggctctgta tcttggcaac
     8101 attacctgat gcaaaggcag aatcagtgcg caaggcttta actgaagctc tgaaatattt
     8161 acctgcagaa ctgcgtaaaa cgttgaccta tgaccgtgga cgcgagatgg cagaacataa
     8221 aatacttgaa gaagatttag gcatagatgt atatttctgt gacccacatt caccctggca
     8281 aaaaggcaca tgcgaaaata tgaatggttt aattaggcaa tatttaccta aagggattga
     8341 tttaaatcag gcagatcagc attatttaaa tcaagttgcc atgtcactga atactcgtcc
     8401 tagaaaagcg ttagattggc ttacaccatt agagaaattt gctcagcttg ttgattatca
     8461 taagactttt caaactgtcg cacctcatgt ttgaattcgc cacgtgttct ttaaagtttt
     8521 caagactttc aatatgatta cttttaatct tctctacttc tataattaat gctttataaa
     8581 ttgaaactga aacatttacc gctttctcgt tagcttctat tattttagtg acaagatttt
     8641 gattttgttc actaaattga tttaaaaatt gtgttgttat gctttctttt acgctgacag
     8701 agacgttttt aacaatctct gtttgtatta aattgctgtt attctgaatt tttttagaaa
     8761 tatattctgt ttgttcatct aaaacattaa gactattttt aatattttta tcaattgact
     8821 ctatttcttc atttaattta tcaatatttt ctttttgact ttgaatatta ttttttgatt
     8881 cattaactgt atttaacagc tctgttaatt caattctttt tagaaaatca aaaatattat
     8941 cttctattgc atcttgagaa tgggttagat gctcattgag ttttttaagt aactcaatgt
     9001 gttcttgttt ttcactctca agtgacattg atagtttgtg tagtgcatct gacatagctt
     9061 ttaatgtcgc tgcaatttgt tgatcgctca taacgaacct ctaatctaaa gggttgaaat
     9121 cattcctcct cgcttgagct aggctctctg cttccgccct gattttggcc tgacgttccg
     9181 cttcaagttt tgtcttggcc tcagcctggg ctttcaaacg ctgctcttct tttgcgatat
     9241 gctcagcttc aagcttagct cttgattcgg cctggagcct ggcctgactt tctgcttctg
     9301 cttgattatt aatgcttgca tcaagttgtg aaaacagtcc tgcttttctc aaacgggaga
     9361 tacttgagtt gagcgtatca aaaatatttt tttcgcttct cgtttcatgt gatttttgaa
     9421 gaatttgatc aaaattttcg aaattagtcg ttttttcttc gcttaaaatg ctctcgaatg
     9481 ttttttttgc ttcgtgctgc attattttga tgagtttttc agtcgcagta atctcaagct
     9541 caagattaaa cagtttgata gcgtcattct gttttgccaa tgctggaagc acgatttcat
     9601 gatttctttc attaaatggc ttttgctgtt ctctctcgta ttgtctacgc aaagcggtaa
     9661 cagatgggcc ttcatgtgac gttgcttgta atccgtggtt ttgatcagca tagctgcgat
     9721 gatcaatacg tacatcaacg ccagcttgct ctaaatgtct gtttgcaaga tctgcaaagt
     9781 cttttcgcca ccggctgact gtttctgtgc catggtcacg actgaaatcc cgatattttt
     9841 ttgctgaaaa ttcaccgttt tcggggtcaa tcgctctagt cgtaaacatg atgtgtgcgt
     9901 gatagttgcg ttcatcagat ccaccatcaa tgtgtggtgc atgaatcgct gcatcaacaa
     9961 tcacgccatg tttttttact aaatcgtgac atagctcatc gagcatggcc tgccgttgtt
    10021 gagcattgag ttctgaagga aaggctattt caaattcacg tcctagcaga gcatcttttc
    10081 tgcgctctga tttttctact gtattccaaa gcgtttgacg atctaataac tcttcttggg
    10141 tattttcggg tgcatatatt tgagtatgtt ctacacctga tttttttgtg tagtcttgct
    10201 ctttgccgta atagtcgcat gtcaattttt cagcagcacg ataagcagca gcagcaacgg
    10261 cactacgacc atcgctccta ctaatcgttt tcactgaaaa atgatagatt gccatactgc
    10321 ttttcccttg cttgttcttt taatttcgct ttagcgaaat taccaacctg aataggttgg
    10381 tcaggggatt acaaagggga tttccccatt gtcgcaccat tgaactttgt tcaatggcta
    10441 agtgcgccct tcggggattc tttgagtatc atatagcgtc ttgaacttca gttcaagcgg
    10501 gattatgatc gagaagaatc caaaagctta agaacggtat ttgattatga gtcttgatta
    10561 tctgaatgaa aaaattgcaa aacaagaaga gcgattgaaa cagttaaaag tgcaaaaaca
    10621 agcaactatt gctcgtgaaa aagcaaagca aaaagaacaa gaccgaaagg atgatacaag
    10681 acgtaaaatt ttgttgggtt cttacttact caaaaagatg gaaaatgaag aaaataagga
    10741 aaaaatactc gctgaaatga acgagtattt aacacaagaa agagacagaa agttatttaa
    10801 tctttaatgt ttgaatagcc actgatttta tagacacctt ttagttagat ttttcagttt
    10861 ttataagaac tactgattag gaccttgaca ccctactttt tactggataa tcttatttca
    10921 acgcagcaaa atctgtgtcc aggcgtgaga acctgataaa atctaggtgc aattaaatgt
    10981 cgcacttgcg gctatttttt tgcctactgt taagcagtcc ctatggtagc ttagcagggc
    11041 agctttgtgc tggctgatcc tagattcagt attctcaccc ctgttaagtt gcctccattg
    11101 ccgtgagaag tgatggagat aattaaactc ttatctagga aactgctatg cagattaaat
    11161 tcaataatgc aaacccatgc atgccaaaaa acctcgctaa acaactgatt gtcgaaagcg
    11221 caaaacttct gaatcatcaa caacgccaag ccttgctcga tattttcatg actttagatc
    11281 aggcggaaag aacagctgaa aatacacttc agcaacatgc tgtagcgcaa caaagcaccc
    11341 acaacttttc tcaagattac gaatgccaag aatacaatca ggaatattgg tcaggtcatt
    11401 actatgattc ttacagtcaa gaatatccgg aagattgggt aaaacggact cagtccaccc
    11461 caggtcgtca taagttccct agaactgctt actgatcgtg gggggtgcct ttttaaaagt
    11521 aggcatcata atccgagcta ctcgaccgag ttggctcgga tgaataccgg ggaaactgag
    11581 ggatttgaga acctgatgac agtcgttgaa ctgcatatta aagaactcaa tacctctgcc
    11641 gcctaaagca ttcactacac gattaatcgt atcaaaacat ggtgcttaat cctgacgtag
    11701 gactttgtgg agtgcctcac cccaatccct gcttctttag cgattttgag tcatgccacg
    11761 agctttagca ataacaccaa attcatattc taaaagcgga catttctact ttgggctact
    11821 gaatttcgta tatgagattt tatgttaaat aggggtaatt aaagattgaa tttcaacccc
    11881 attttcatca actaattcaa taccaactgc ttttaaacga tctataggat cacctgtttt
    11941 caaacaccat ccattaggca tatttgtttt taatcctgaa aatttagtat ctaacttttt
    12001 ctgttcttca taggaaatat gggctacacc aagatttctt tgtagaattt tggagagttc
    12061 agcatctgat aattcattgc tttcaattaa tacccataag ttgttaaaga cataagccat
    12121 gggaacaata tgttctttcc atggcttaat agccattgct gatttattat taaaaatatt
    12181 ctttgagtat cctttaaaaa gcagttcatt ttctggatga aataaatgct ccattaatct
    12241 ggagctatat ccccatggtt tattaatgtt ttcttgaaga gtgataatgt tttttacatg
    12301 ttgaatgatt aaatcacacg tgctcttaat atattgatta atatgttctt tttcagtcat
    12361 gttggttttc caaaaaatta ctgctacctt agcgatttta ttatagtgat aaaatgagta
    12421 atcttaagaa agttacttta atccaaacag atattacaac attcgctgta caggctatcg
    12481 ttaattcagc gaataagtct ttgcttggtg gcagtgggct tgattatatc attcataaaa
    12541 aagcaggacc tttgatgaag actgaatgta ttcaacttca tcaagaaaaa ggcgggtgtt
    12601 caactggaca agcagaagtt actactgctg gaaacctgcc tgcccaatat cttattcatg
    12661 ctgttgggcc aagatggtta gatggtgagc gtaatgaacc acaattgctt tgtgatgctt
    12721 atagtaatgc gattatgaaa gctaaccatg ttggtgtaaa aactatttct tttccaaaca
    12781 tcagtacagg tgtttatggt tttcctcgcc aactggcagc cgaaattgca attggtacca
    12841 tactttcaac acttccccaa tatgactgtg ttgaagaagt attttttgta tgccgagatg
    12901 atgaaaacta tttaatttat aaggagatac tttctaacat taacgaccca aaaataaaaa
    12961 tacagattga ataaattaaa ttatagatag ctcatataag aatcctgcta ccagtttggc
    13021 taaaagctca tgatcttctt gggattcaac gatatcaacc attaaatcat ctaaacccgt
    13081 gtgttcttga atggtcacac cttggatttc aagataagtt agcatcactg ctagccctgt
    13141 tcttttattt ccatcgacaa atgcatgccc tttagaaatt gcaataccaa accatgcagc
    13201 aatttcaaag atatcactca cattactgta atacatttgc tgatctatac ggtttaggac
    13261 gctttccagt ttctcaatct ggcaaccaga tcgacctaca cctgtttgaa gcaaaatctc
    13321 gtcatgtaca gcaattacga atgtcgaact aat
//
