LOCUS       NZ_CP137699             3327 bp    DNA     circular CON 08-NOV-2023
DEFINITION  Escherichia coli isolate FELIX_MS533 plasmid pEcFELIX533.3, complete
            sequence.
ACCESSION   NZ_CP137699
VERSION     NZ_CP137699.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37709341
            Assembly: GCF_033395675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Wan,K.H., Park,S., Neff,M., Lawrence,Q., Eichenberger,J.P.,
            Booth,B.W., Piaggio,A.J., Chandler,J.C. and Franklin,A.B.
  TITLE     Complete Genome Sequence of the Escherichia coli, FELIX_MS533
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Wan,K.H., Park,S., Neff,M., Lawrence,Q., Eichenberger,J.P.,
            Booth,B.W., Piaggio,A.J., Chandler,J.C. and Franklin,A.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Life Sciences Division, Lawrence Berkeley
            National Lab, 717 Potter Street, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 63.62x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/08/2023 07:00:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,681
            CDSs (total)                      :: 4,557
            Genes (coding)                    :: 4,311
            CDSs (with protein)               :: 4,311
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 65 of 246
            Pseudo Genes (incomplete)         :: 176 of 246
            Pseudo Genes (internal stop)      :: 49 of 246
            Pseudo Genes (multiple problems)  :: 43 of 246
            Pseudo Genes (short protein)      :: 3 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137699.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="FELIX_MS533"
                     /db_xref="taxon:562"
                     /plasmid="pEcFELIX533.3"
                     /genotype="IN(emrR)49kb"
                     /note="derived from Escherichia coli JCC-EE 14; 12 weeks of
                     evolution under tetracycline selection"
     gene            join(3144..3327,1..254)
                     /locus_tag="R6I08_RS23395"
                     /old_locus_tag="R6I08_23395"
     CDS             join(3144..3327,1..254)
                     /locus_tag="R6I08_RS23395"
                     /old_locus_tag="R6I08_23395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046597188.1"
                     /translation="MKLKGFWLSAIVIAACNGMGFLFLYLSKTYFFPMVTFKDNLGNLG
                     SSLVTYSTAMMALLIAMVVIIFGLDGPRLHNFKKSGYLHVTYFIYMITFFELGLTMALS
                     LLCLSNISSIKVASYSLTFAFITFLLIGCLGIQLLNIKKNL"
                     /name="hypothetical protein"
     gene            1241..1390
                     /locus_tag="R6I08_RS23400"
                     /old_locus_tag="R6I08_23400"
     CDS             1241..1390
                     /locus_tag="R6I08_RS23400"
                     /old_locus_tag="R6I08_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_163396971.1"
                     /translation="MPSEYDEQRDATDALLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2300..3133
                     /locus_tag="R6I08_RS23405"
                     /old_locus_tag="R6I08_23405"
     CDS             2300..3133
                     /locus_tag="R6I08_RS23405"
                     /old_locus_tag="R6I08_23405"
                     /inference="COORDINATES: protein motif:HMM:NF027299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072775497.1"
                     /translation="MILLAGKKMKTNYFTHRLFPSDGAVICEPLKTSFDNMIATNRNTA
                     TLANEYHVIQHVSGDVYTFIKTNSRDVFRKLDTSSNICVDLSNILNQHEKIAFASFFIL
                     KGDVIGFSNTLYSPRVGKLAEIYDAAMYSRNGNHNINFEPITNIVTEQDVMNYAHVGKI
                     TMKIEKSQGIIGGLGTLFSGNVKYDDVDSFEIKIIPKRAKDIKDTFAGLMQARPQEVSS
                     VAVSAKEHIGDVATDLNVIMSNTVYDFVNPNDTTTIEVQMEKNYNNNTTLRSLGY"
                     /name="hypothetical protein"
     Typing          1034..1248
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.364"
                     /coverage="100.0"
                     /evalue="1.6000000000000006e-59"
     Typing          complement(572..1084)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.827"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 tggttgttat aatctttggc ttggatggac ctcgcttgca taactttaaa aaaagcggct
       61 atctccatgt tacatatttt atttatatga taacgttttt cgagctcggc ttaaccatgg
      121 ctctttcgtt actttgtttg tccaatatct caagtattaa ggtggcttct tattcattga
      181 cttttgcatt tattactttt ttgttgatag gatgtttagg tattcaactc ttaaatataa
      241 aaaagaatct ttaatcaacc aaactgtgat gttcatcaca ggtgaaacgg tttgtgacca
      301 tttttggtga gttgataaaa tgcagaatgt gtaaggatcg tcttgagatt tttgagagtg
      361 cagcgtagtc tcttgctctg taaacaaaaa aaccaccgct accaacggtg gtttgtttgc
      421 cggaagtgag agctaccaac tctttctccc gaaggtaact ggctttcacg gagcgcagat
      481 accaaaactg tccttttagt gtagccgtag tcaggccacc acttcaagaa ctccatagaa
      541 atactctact actctgctcc gtgaatcctc ttaccaatgg ctgctgccaa tggcgcgttg
      601 tcgtgtcttt ccgggttgga ctcaagaaga cagttaccgg aaaggcgcag tagtcggact
      661 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
      721 tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
      781 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
      841 cctgtcgggt ttcgccgcca ctgatttgag cgtcagattt tgtgatgctt gtcagggggg
      901 cggagcctat ggaaaaacgg cagcgccgta tcctttttcc ggcctttcct gacttgagtg
      961 ccatcttttt atcttgtcat tcgtgagcag gtaacgcccg ccgcagtctc acggcaggag
     1021 cgtagcgacc gagtgagcga ggaggcgtca tatcaccgta actcccattt tctcccttac
     1081 gccctgtagc tgcattttcc cctgtcacat gaagcacttt cattaggtgt tactccgccg
     1141 taacatattc agccactgta cactccgcta gcgctacgtg actggttcag ggctgcgccc
     1201 cgaaacccgc taacacccct cagctactgg atatcacaac atgccatctg agtatgatga
     1261 acaacgggat gcgactgatg ctctgcttcc cggcgaatat gacctttttg atttaatggc
     1321 gcaggttact gaagagaacc tgcacgcccc ggtggatttt gggccacctg tcggccgtga
     1381 agcgttatag ctttttcgct tacccgcgta cgtgtaatac gccggtatta catcttattt
     1441 ctttattttc atgtgattac aggtggttgt aatacgttgt gtattacata aataaacggg
     1501 aacttcagtt tttacctttt aattcatgca attgcgatga aaatcgcggt tgcgtatggt
     1561 gtatgaccat tttaaggttg aaaaataatc aaccatagag ggtgatttac gcaggttaag
     1621 ggaagatcgt gacaaaaaaa gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt
     1681 attcttcctg actacagaat tacatcaaaa gtacatcaaa attacatcac ttttacgtca
     1741 gatcgcgggt gtacaacaga attacatcaa aagtacatca aaattacatc acttttacgt
     1801 cacttttaca tcatttttac gtcagatctg acctctgaac tattcgtaca tccgcccctc
     1861 ttcctgggcc tcctgcacgt gctgaaaatg gtttttccgg ctgtgccggc aacgcctcac
     1921 ggccacgcaa taaattgcgc atccgttcac cgttatcggg gtggttcgtg gtggctttca
     1981 gggcgttcat cggattttag ccgctaaaat cccggccagg tatgtccgtg catagccatg
     2041 cagtcgcgcc agaatcatca ggggcgtttt ttctcgatat gggcacagaa aggtgtgtgt
     2101 ttcaggccgg aggcctcaga aacgcgctga gggcctctgc ggcggtgcac gcaacggatg
     2161 ttatggtaaa tagagttggt ggcctagctt tacgaccttc ggtgacagat catttaatat
     2221 gcaaaacttg gaattttaaa gaagcattgt tcttccataa gaaatagaat ataaaaagaa
     2281 gaaaatataa tgcgtttcta tgattttact agctgggaaa aaaatgaaaa caaattactt
     2341 cacacacaga ttgtttcctt cagatggcgc tgttatttgt gaaccattaa aaactagttt
     2401 tgataatatg atcgctacta acagaaacac tgcaacgcta gccaatgagt atcatgtaat
     2461 tcagcatgtg tctggcgatg tatatacgtt tataaaaaca aatagcagag acgtttttag
     2521 aaaactagat acgagtagta atatatgtgt agatctctca aacatcttaa atcaacatga
     2581 aaaaatagcc tttgcatctt ttttcatatt gaagggtgat gttatagggt ttagtaatac
     2641 gttgtactct cctcgtgttg ggaagttggc agaaatatat gatgctgcaa tgtattcccg
     2701 aaatgggaac cacaacataa attttgagcc gataacaaac atagttaccg agcaagatgt
     2761 tatgaattat gcccatgttg gtaaaataac gatgaaaata gagaagtctc aaggcattat
     2821 tggtgggctt ggtacgcttt tctctgggaa cgtaaagtat gacgatgtgg attcgtttga
     2881 gataaagata atccctaaac gggctaaaga cataaaggat acctttgcag gtttgatgca
     2941 ggctaggcct caagaggtta gctcggtcgc tgtgtcagct aaagaacaca ttggtgacgt
     3001 tgcaacagat ttgaatgtta taatgtctaa taccgtatat gattttgtaa atccaaatga
     3061 cactactact atcgaggttc agatggaaaa aaattataat aacaatacaa cattaagaag
     3121 tttaggttac tgaggattgc attgtgaagc ttaagggttt ttggttgagc gctatagtta
     3181 tagctgcatg taatggcatg ggctttttat ttctatacct ttcaaaaacc tatttcttcc
     3241 cgatggtaac gtttaaggat aatttaggta accttggttc ttccttggtt acttattcga
     3301 cagcgatgat ggcgttgctt attgcta
//
