LOCUS       NZ_CP137479            12945 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter portucalensis strain F321 plasmid pF321-3, complete
            sequence.
ACCESSION   NZ_CP137479
VERSION     NZ_CP137479.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37933146
            Assembly: GCF_033232945.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Li,X.
  TITLE     Citrobacter portucalensis F321 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12945)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 16:48:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,370
            CDSs (total)                      :: 5,250
            Genes (coding)                    :: 5,147
            CDSs (with protein)               :: 5,147
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 28 of 103
            Pseudo Genes (incomplete)         :: 74 of 103
            Pseudo Genes (internal stop)      :: 18 of 103
            Pseudo Genes (multiple problems)  :: 16 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137479.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="F321"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1639133"
                     /plasmid="pF321-3"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2021-05-19"
                     /collected_by="Jiayao Yao"
     gene            1..879
                     /locus_tag="R4T15_RS24595"
                     /old_locus_tag="R4T15_24595"
     CDS             1..879
                     /locus_tag="R4T15_RS24595"
                     /old_locus_tag="R4T15_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_009873352.1"
                     /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFALE
                     AAMSIACSVAGGDLVGLAPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIAEN
                     LMLEPIMGKRLNVMDEAHLRRVIDYSAGARLIVLDTLSRIHSLDENSNGDMAHLVSVLE
                     HIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQEAERL
                     SDRGFDRAPIGNERRGLFVRFGVSKQNYDATPLDRWYQRHTGGVLLPVELQEIKRESGN
                     KGGKGYDSAAY"
                     /name="helicase RepA family protein"
     gene            860..1852
                     /gene="repC"
                     /locus_tag="R4T15_RS24600"
                     /old_locus_tag="R4T15_24600"
     CDS             860..1852
                     /gene="repC"
                     /locus_tag="R4T15_RS24600"
                     /old_locus_tag="R4T15_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_009873351.1"
                     /translation="MTQQRIKPAYAKFDPAHVFDGLFVPTKGKKRGRLYVPPRRFGDSE
                     ISFQGFEQLGADDQSILLAVSAQLGIDGLMIEAKPPGEIGQQLRLALDFDNDDGRPLKT
                     KKTSLRSLLIDAGYDDPESGAALARIKESLNRMGNAQIREINHATGWDYRCNLLSSRFN
                     HKTGEIFVAVNPRLTGAVFREQHVKVSLFERNELESEAAKLLHCWLCSNIRLGRSLGNG
                     NGAHIDTLAPHVWGKAAWEEASTKVRSVRRGQLRDALDEISDRTRGLQGGMGWAIDQTS
                     SGLVLVSRPKELPFLEGEHDMRPSEYHDLMSQPPMQEPDLFDPQDWTPD"
                     /name="repC"
     gene            complement(1905..2672)
                     /locus_tag="R4T15_RS24605"
                     /old_locus_tag="R4T15_24605"
     CDS             complement(1905..2672)
                     /locus_tag="R4T15_RS24605"
                     /old_locus_tag="R4T15_24605"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_238706593.1"
                     /translation="MGVDRGVAIPVCAGEQTYDFTHSQKKNMSKADRYIKRLQRKLARQ
                     QKGSNRRAKTKHRIAVHHAKKANIRNDFAHKTSRLLVDSSAKIIVFEALRTARMTRRPK
                     AKQDEQGRFISNKAKQKAGLNKSILNVGWHVIETYTYYKAYSSSKAVFKIPAPTTSQEC
                     AKCGHTHPDNRKTQALFVCGNCGNTDNADHNASLVIKKRAINLILDTGTVLSGDGVLRT
                     QSDSGRGGNRKTGRAKSSTSSAQRSVKKEKLAA"
                     /name="transposase"
     gene            <2713..2811
                     /locus_tag="R4T15_RS24610"
                     /old_locus_tag="R4T15_24610"
                     /pseudo=""
     CDS             <2713..2811
                     /locus_tag="R4T15_RS24610"
                     /old_locus_tag="R4T15_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3001..3885)
                     /locus_tag="R4T15_RS24615"
                     /old_locus_tag="R4T15_24615"
     CDS             complement(3001..3885)
                     /locus_tag="R4T15_RS24615"
                     /old_locus_tag="R4T15_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(3941..5416)
                     /gene="msr(E)"
                     /locus_tag="R4T15_RS24620"
                     /old_locus_tag="R4T15_24620"
     ARG             complement(3945..5416)
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6253..7005)
                     /locus_tag="R4T15_RS24625"
                     /old_locus_tag="R4T15_24625"
     CDS             complement(6253..7005)
                     /locus_tag="R4T15_RS24625"
                     /old_locus_tag="R4T15_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873360.1"
                     /translation="MRIKIKGEITAERLAEALHAAAEKYEAVRPGHKVYGANLYLTAFD
                     ADGLPFDLVDHRGEPLSITIEAKSGELVKPALTAEGEAHRQKAKEEARRQAEEAEAEAQ
                     RRHRQTLDEYEQERQKRRKKEAEARKQFEDANAITAELLKTMPERFIDELNKTVQGVWD
                     DLKPTETQGKKKGQPKALPVFSIHADGLVLSVETWKNPRRVLNPLCTLQHGEIAPFWMH
                     EAWLEAMRRIVDLLDTLTAAPAEALESQ"
                     /name="hypothetical protein"
     gene            complement(7002..7640)
                     /locus_tag="R4T15_RS24630"
                     /old_locus_tag="R4T15_24630"
     CDS             complement(7002..7640)
                     /locus_tag="R4T15_RS24630"
                     /old_locus_tag="R4T15_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6753 family protein"
                     /protein_id="WP_009873359.1"
                     /translation="MSDLDDSFAKLLGRQPSDAERQSLYRVRDALGLKNNDALWLVLMA
                     LQHYQGQYEKFPQAIAQAAKDTLVNFKVTADATVKASAEAAKADLAQAVAAAAQEVAHN
                     TSAKQMWQWAAGCIAVAFLCVGLFGWYMHSSGKDSGYQAGYGAGYTEAKDEKAAAAWAN
                     TPEGRLAYRFAQTGSLASLAKCDRPGWYVEKGVCYVKPASDGTYGWRLP"
                     /name="DUF6753 family protein"
     gene            complement(7633..8316)
                     /locus_tag="R4T15_RS24635"
                     /old_locus_tag="R4T15_24635"
     CDS             complement(7633..8316)
                     /locus_tag="R4T15_RS24635"
                     /old_locus_tag="R4T15_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mobD"
                     /protein_id="WP_009873358.1"
                     /translation="MAKSIYLIGGSKGGVGKSLVTMATVDYLQERGESVLLIESDTSNP
                     DVWKAYKESTETELINLDEADGWIQLVNLCDSKPDSVVVINTAARNNKGVSAYGETLNS
                     TLEELKRKLVTLWVINRQRDSLELLKEYMDAIPNADVHVVRNGHFGEEKKFELYNGSKL
                     RTAVEERGGKSVTFPDMADRVSDDIYSKRMSIAVALKELPIGNRAELTRWRNEAKKVLE
                     AVVHE"
                     /name="protein mobD"
     gene            complement(8329..8685)
                     /locus_tag="R4T15_RS24640"
                     /old_locus_tag="R4T15_24640"
     CDS             complement(8329..8685)
                     /locus_tag="R4T15_RS24640"
                     /old_locus_tag="R4T15_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873357.1"
                     /translation="MKYTTEQLEAIASKLREMPPVEKKKQEHSKQEAVRVLSKEIAALQ
                     KRGYTLDQISETLRGEGLSIATPTLKSYLQRAKPAKKAPVQAPGDTPPPRPADKKPADT
                     SKATFTPKPDSDDI"
                     /name="hypothetical protein"
     gene            9007..9318
                     /locus_tag="R4T15_RS24645"
                     /old_locus_tag="R4T15_24645"
     CDS             9007..9318
                     /locus_tag="R4T15_RS24645"
                     /old_locus_tag="R4T15_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003869.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_009873356.1"
                     /translation="MPFETQGPEPLDAVINVRLTAAEKARLKEDADLAGLSMSELVRRR
                     YFGRPIIANADAVMLKELRRIGGLLKHIHNESGGVYSKETAGALVALKDYFRKLNDDR"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            9308..11980
                     /gene="traI"
                     /locus_tag="R4T15_RS24650"
                     /old_locus_tag="R4T15_24650"
     CDS             9308..11980
                     /gene="traI"
                     /locus_tag="R4T15_RS24650"
                     /old_locus_tag="R4T15_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="WP_284302396.1"
                     /translation="MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGARG
                     FMMDDPKSQTAEMIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGWK
                     DHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNRGFDIELAHKAIARIEHAQGWQRE
                     QNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAIIKQAQT
                     WEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQ
                     QVAPREPEPIKPDVPGWKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDE
                     LMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQ
                     KSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVS
                     FVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVMGNDEYKAMCGKLAAEHGFKITNP
                     ELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRVTSIKMREDGSKQTFIL
                     DKKDSITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIR
                     DGYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRA
                     PGYENRKPKHQREDGSYPEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSK
                     AKPALELAAASGSAIDAYQRHYRDVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEG
                     AIRQCAPATRQKDEGRDWNDYAQRTARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQ
                     QEQAKAQKIERDNSPGMSL"
                     /name="traI"
     gene            12047..12271
                     /locus_tag="R4T15_RS24655"
                     /old_locus_tag="R4T15_24655"
     CDS             12047..12271
                     /locus_tag="R4T15_RS24655"
                     /old_locus_tag="R4T15_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="WP_043149934.1"
                     /translation="MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQC
                     LLVAQADSADTAMQQFMDEALADVDGWTE"
                     /name="antitoxin MazE family protein"
     gene            12268..12594
                     /locus_tag="R4T15_RS24660"
                     /old_locus_tag="R4T15_24660"
     CDS             12268..12594
                     /locus_tag="R4T15_RS24660"
                     /old_locus_tag="R4T15_24660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303585.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_009873354.1"
                     /translation="MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLN
                     AAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSKVGPAFGRIDADALLEVERCLAVFL
                     GIAK"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            12591..12917
                     /locus_tag="R4T15_RS24665"
                     /old_locus_tag="R4T15_24665"
     CDS             12591..12917
                     /locus_tag="R4T15_RS24665"
                     /old_locus_tag="R4T15_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873353.1"
                     /translation="MKTIYTIPAPDSLGAMIEVYGEPENAWYEWRIIDGGRTVRDTGTE
                     GHSAFQGRQYGQAEIALRDALMFASGLPIDGDTPTDAASLEAGYAAKAKAEAARQGAGT
                     PGSA"
                     /name="hypothetical protein"
     ARG             complement(3005..3885)
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3942..5416)
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3002..3885)
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..879
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9309..11977
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.888"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctttag acattatggc ggccttcacc aatgagccgc cagaacttga tttcatctgg
       61 cccggattct tggccggaac cgtgggcgca cttgttgcac ctggcgcaac tggcaagagc
      121 ttttttgctc ttgaagcggc catgtcaatc gcttgcagtg tggcaggcgg cgaccttgta
      181 ggactggccc cggcgcacac cgggcgcgtg gtctatctcg ctggcgaaga tccacagccc
      241 gcccttgtgc ggcgtgtcca cgccatcggc cagcacctca accagtcggc ccgcgaagcc
      301 atcgctgaga acctgatgct tgagccgatc atgggcaagc ggttgaacgt gatggacgag
      361 gcgcacttgc gccgcgtcat cgactacagc gcaggggcgc ggctgattgt gctggacaca
      421 ttgagccgca ttcacagcct cgacgagaac agcaacggcg acatggccca ccttgtttcc
      481 gtgttggaac acatcgcggc aaccaccggg gcggctgtcc tgtacctgca ccacgtcaac
      541 aagggcagcg cccgcgacgg ccagaccgac cagcagcagg cagcgcgggg cgcgtctgcc
      601 ctgatcgaca acgccagatg gtgcggctat gtcgccaaga tgacggagca ggaagccgag
      661 cgcctgagcg accggggctt tgatcgtgcg cccatcggca acgagcggcg cggccttttt
      721 gtccgctttg gcgtgagtaa gcagaactac gacgcgaccc cgctagaccg ctggtatcag
      781 cgacacaccg gcggggtgtt gctgccggta gagctacagg agatcaagcg tgagagcggc
      841 aacaaggggg gcaagggcta tgactcagca gcgtattaaa ccggcctatg ccaaattcga
      901 tcccgctcat gtgtttgacg ggcttttcgt ccccacgaag gggaagaagc gcgggcgact
      961 gtatgtgccg ccgcgccgct tcggcgatag tgaaatcagc tttcaagggt tcgagcagct
     1021 tggagctgat gaccaatcaa tcttgcttgc agtaagcgcc cagcttggca ttgacggctt
     1081 gatgatcgag gcaaaaccac ccggcgaaat aggccagcaa ttgcgccttg ctttggactt
     1141 tgacaacgat gatggaaggc cgctgaaaac caagaaaacc agtttgcgca gcctgctgat
     1201 tgatgctgga tatgacgacc cagaaagcgg ggcggcactt gccagaatca aggaaagcct
     1261 gaaccgaatg ggtaacgcgc agatacggga aatcaatcac gctaccggct gggattaccg
     1321 ctgcaatctc ctttcgagtc ggttcaatca caagactggg gaaatctttg ttgccgtcaa
     1381 tccgcgcctg accggggcgg tttttcgaga gcaacacgtc aaggtttcgc ttttcgagcg
     1441 caacgagctg gagagtgaag ccgccaagct gctgcactgt tggctgtgct caaacatccg
     1501 gcttggccgt tcgcttggca atggcaacgg ggcgcacatc gacacgctag cgccccacgt
     1561 atggggcaag gcggcgtggg aagaagcatc aacgaaggtt cgatcagtca ggcggggtca
     1621 actacgcgat gctttagacg aaatctcaga ccgcacacgc ggactgcaag gcggtatggg
     1681 atgggccatc gaccagacca gcagcgggct tgtgctggta agtcgtccca aggaactgcc
     1741 gttccttgag ggtgagcacg acatgcgccc cagcgaatac cacgacctca tgagccagcc
     1801 gccaatgcaa gaaccagatt tgttcgaccc gcaggactgg acgccggact gagccgcacc
     1861 tggtgaacta ctcgctgcta aagcggtcga gcttccaaac taacctaagc ggccagcttt
     1921 tcttttttga cgcttcgttg agcactgctg gttgagcttt tggctcggcc agtcttacga
     1981 ttgcctccac gtccactatc cgactgagtt cgcagaaccc catcgccaga taacaccgtt
     2041 ccagtgtcca aaattaagtt aattgcccgc ttcttgataa ccagtgatgc attgtgatct
     2101 gcattgtcag tatttccaca attaccgcaa acaaacaggg cttgtgtttt gcgattgtcg
     2161 ggatgagtgt gaccgcattt tgcgcactcc tgactggttg ttggtgcggg tattttaaat
     2221 accgctttgc tggatgaata agccttgtag taggtgtaag tctcgatcac atgccagccc
     2281 acattaagaa tagacttatt cagtcctgct ttctgcttgg ctttatttga tatgaatcga
     2341 ccttgttcat cctgttttgc tttcggcctt ctcgtcatcc ttgcggttcg taacgcctca
     2401 aacacaatga ttttggcgct gctatcaacc aataaccgac tggtcttatg cgcaaaatca
     2461 tttcgtatgt tagccttttt agcatgatga accgcaattc gatgcttggt cttggctctg
     2521 cggttagagc ctttttgctg ccttgccagt ttgcgctgca atcgcttgat atagcggtct
     2581 gctttgctca tgttcttttt ctggctatgg gtgaaatcat aggtttgttc gccagcacaa
     2641 acggggatcg ccacgcctcg gtcaacaccc atggtgtgct cttccagata ttctctggtc
     2701 gccccttgca gaaaagaatt gctagtagtt ggcaatatga ctaaggaaga aattgccaaa
     2761 gcagtgaatt gtggagttgc aactgtctat cgagttgcta aagttatcta aaagaggctt
     2821 atctcaacgc gcccccaatg gggcaccgct gcattcaggt tacttcacat aagaaatttt
     2881 atgttaaatg attgttagaa atgccccttg atcgaggcat tttctataaa gaatactaaa
     2941 aactaattaa agccaaccaa gtttcaaaac aaggcagttc tcagcaatca tatttttaaa
     3001 ttatataact cccaactgag cttttgctcc aacgataagg ctttcatttt gagtttctaa
     3061 ggcaaataaa ccatacatca aaggagaggc cgctgctctt tctaaagtct gttcatatag
     3121 tttattccaa actttacccc ctagtttttc atactcgatg attagagttt tgaggctttc
     3181 ttctccaaac aaagttacat gcccagcaaa atcaatcgct gggtcatcta tatgggctgt
     3241 tgaccaatca ataacgcctg aaacagctcc atcctttgaa gctagtacat gcccagcata
     3301 taaatcgcca tgtataaatt gggtgaaatc tgcccataga acatcattat ccaaccattt
     3361 tctgtagcgg gtttccaatt gctcacttat accaatttca gattttacta actgcaaatt
     3421 gtttgctatt tcaggtctta aatctgaagg tttcataatt ttcaaatcat tttcccgaac
     3481 ttctttttca ggaatactat ggatttcaaa taaggttttt gccaaagatg ttatgtattt
     3541 cgggctatct ttgtccatat tccaaattat ttcataggtt tcagcatcca aatttaaaac
     3601 aggattatct ttaagtatgg gataagccac taattctgta gatgaaattc tccaatcagg
     3661 aacctctaca gaaagatgtt tttttaccaa ttctaaaatg cgtttttctt tcttgatttg
     3721 ttccctcatg ccatcacgac gaggaatacg cagcaaccat tgttgcccct ttgtatcaag
     3781 agcaaaaacg accttaaaat caatgcccat ttcattgaaa ttcattttgt ccgtaagcaa
     3841 caagccgtgt gcttcagcaa gtgattgaat atcttgaatt gtcattttta atttccttta
     3901 aagagttcaa taattaatgt tcggattaga ttggctatca ttaacaatct ctctcaaaag
     3961 tcttgatgat tttgtggtct ttgatctcgt agataatgtc agcaatatta tcgaccaatt
     4021 gcttgtcatg agatacgaag ataatagttc ctgcatagga cttcatcatt gtttctaatg
     4081 cggcaatact ttttaggtca agatagtttc ctggttcatc cataagcaaa atattatatt
     4141 ttcctaaaag cattttggat aaaagcagtt tgatgatttc acctcccgat aagtcggata
     4201 agtttttttg aatatcattc gctccgatcc ccattgaagc caatactgca cgaatttccg
     4261 caactgtgta ctcgcactct tcctgcataa aggagagcac agatttatgc gtgttaaatt
     4321 tatatcctgt ttgtgtaaag tagccaattt cagcttttgg agatatggtt aatccatcag
     4381 cacgttctga tatcattttt aacaaggacg ttttccctgt tccattcgat ccagttatag
     4441 cgactttagc gccaagcggt attataaagt tagcgtcatc aaagatagta cggctaccaa
     4501 attttaagct cagaccatct gccgtaatcg ggaacttatt gtgcagttct agggctgaac
     4561 tttgacgaaa acgaatagaa cgcaaatgct ctggtgcttg aatatcttct aatgcagcca
     4621 aacgcttttc catactctta gctgcctgat acagttttct ttgcttggtg ccagtcattt
     4681 ttgcatgccc aagtcgtcca gcactttcgg tagagttttt ggatttttct ccttttttct
     4741 tattgtctaa tcgattagct tgctggcgtt tttcttgcac agcagattct aatcgctccc
     4801 gttccttcat catcagctca tattctacgg cttggtgttg tcgctcttct tctttttgac
     4861 gcaagtaatc cgagtaacca ccccaatatt ccgtaatttt accgtctttt aactcccata
     4921 tcttgtctac aaccatatca agaaaatatc ggtcatgact gataacaagt aatgctccat
     4981 caaatgcttt aagttgacca ataagtagat ctattccatt gagatcaagg tggctggttg
     5041 gttcatccgc tagaatgcca tgtacttgtt gggaaaatgc ggcagcaatt tttgcacgag
     5101 tttcctctcc gccactcatt gtgtcgtttt gtacattgga aacaccaagg cgagataaca
     5161 ttgcccggtc ttcgaccgtt tctatttcga ttccgcccag ttggctgata tgtgcaaaat
     5221 caccaaaacg ctgtaatgtc gcttcggcta aaacaatttc gccattaagt actttgagta
     5281 aactactctt tcctgctccg ttatcaccca caagaccaat acggtcataa gagtgaattt
     5341 ccaattcatc aatatccaaa acatcacgcc cagcataatc caagcgtatg tttctcgctt
     5401 taataattaa actcattttt atttactcct gtttagctct tgaaattttt tatgcagcaa
     5461 acaggattta ggtgaaaaca aaagctagca tcacagtgtc ctcccaaaaa aaaagctatt
     5521 catccacagg gtggacaaat agctagtcaa ttaagttata actggaaact atgcactaaa
     5581 agcatactta taaattaagc atacttttac tttatatatc cgcatatatt cttaaagaca
     5641 caacaaaagc ccaccattat aaaatagtgt cactatgcaa atagttgtgt cttaacgaat
     5701 gcggatagaa tgcataaact tacctaaaaa ataaaattca gttttatgat aactttactg
     5761 ttaaaagaag tctagacaac cttgttttat gcttggatat aaggcttatt ttaaaataat
     5821 agtagtgaag attttctaaa attaacataa tacaccttaa gtgtagtgtg tttgtctatc
     5881 aataagagat ttatctaatt taattcaagc ccccggcgca ggtggtaacc acccccggcg
     5941 caggtggtaa ccacccccgg cgcaggtggt aaccaccccc ggcgcaggtg gtaaccaccc
     6001 ccggcgcagg tggtaacctg acaagccaca aaaacgcccg caaacccttg tcagtactga
     6061 gttttcgcgt ttttccaaaa ttccggtcta agaagttcta aagaacttct accatgcgcg
     6121 gctttcagcc gccgctttag gcgtcgtctg aagccttgcc ttcgggcttc gccctgcgcg
     6181 ggctaaagcc cttgcagaca cacccccgcc cccgccccgc cgctaccccc ttccttgcag
     6241 gcgaaaacgc ctctactggc tttctagggc ctctgccggg gctgcggtga gcgtatccag
     6301 cagatcgact atccgcctca tggcttctaa ccacgcttcg tgcatccaga atggggctat
     6361 ttcgccgtgc tggagtgtgc aaagcggatt gagaacccgg cgcgggttct tccatgtctc
     6421 gacggaaagc accagcccgt ccgcatggat ggaaaagact ggtagcgctt tcggctggcc
     6481 cttcttcttg ccttgcgttt cggttggctt gaggtcgtcc caaacgcctt gcacggtttt
     6541 gttcagttcg tcgatgaagc gttccggcat ggttttcaag agttcggcag tgattgcgtt
     6601 cgcgtcctca aattgctttc ttgcttcggc ttcctttttc cgtcgttttt gccgctcctg
     6661 ttcgtattcg tctaaggttt gcctgtgtct gcgctgtgct tcagcttccg cttcttcagc
     6721 ttgccttcgt gcttcctctt tggctttctg tcggtgtgct tcaccctctg ctgtgagtgc
     6781 tggttttacc agttcgccag acttggcctc gatggtgatc gaaagcggtt cgccgcgatg
     6841 gtctaccaga tcgaacggca gaccatcagc atcgaaggcg gttaggtaga gattggcccc
     6901 atagaccttg tgccccggcc tcacagcttc gtatttttct gctgctgcgt gcagtgcttc
     6961 cgctaggcgt tctgccgtga tttctccttt gatcttgatt ctcatggcaa cctccatccg
     7021 taggtgccat ccgatgcggg cttgacgtag cacacgcctt tttcgacgta ccaccccggc
     7081 ctatcgcact tcgccaagct ggcgaggctt cccgtttgcg cgaagcggta ggccaagcgc
     7141 ccctctggcg tgttggccca tgccgccgcc gctttctcgt ccttggcttc ggtgtatccc
     7201 gcgccgtatc cggcctgata gcccgaatcc ttgccgctgc tgtgcatgta ccagccgaac
     7261 aggccgacgc acaggaaggc caccgcgatg cagcccgccg cccactgcca catctgcttt
     7321 gccgatgtgt tgtgcgcgac ctcttgcgct gctgctgcta ccgcttgcgc caaatccgcc
     7381 ttggctgctt ccgctgatgc tttcacggtt gcatccgccg ttaccttgaa attgaccaag
     7441 gtatctttgg ccgcttgtgc tatcgcttgc gggaacttct catactggcc ttggtagtgt
     7501 tgcaaggcca tgagcaccag ccacagcgca tcattgtttt tcaggccgag cgcatcccga
     7561 acccggtaaa gactttgccg ctccgcgtca ctaggttgcc tgccgagcag ctttgcaaag
     7621 ctgtcgtcta ggtcactcat ggactacggc ctccaatacc tttttggcct cgttgcgcca
     7681 ccgcgtaagc tccgcacggt tgccaatggg cagttccttg agtgctaccg caatgctcat
     7741 gcgcttgctg taaatgtcat cgctcacacg gtcggccatg tccgggaatg tcaccgattt
     7801 cccgccgcgt tcctctaccg ccgtgcgcag cttggagccg ttgtaaagct cgaacttctt
     7861 ttcttccccg aagtgtccat tgcgtaccac atgaacatcc gcattcggaa ttgcgtccat
     7921 gtattccttt agcagttcga ggctatcccg ttggcggtta atcacccaca gcgtgaccag
     7981 cttgcgcttg agttcttcaa gcgtgctatt cagtgtttca ccgtaggcgc tcacgccctt
     8041 gttattccgt gctgccgtgt tgattaccac gacgctatcc ggcttgctat cgcacaggtt
     8101 cactagctga atccatccgt ccgcctcatc cagattgata agctccgttt ccgtgctttc
     8161 tttataggct ttccatacgt ccgggttaga ggtgtcagac tcaatcagca agacgctttc
     8221 gccgcgctct tgcaggtaat caaccgttgc catcgtaaca agcgacttgc caacgccgcc
     8281 cttgctgcca ccgatcaggt aaatgctttt cgccatgttg cttactcctt aaatgtcgtc
     8341 gctgtcaggt ttcggggtga atgtcgcctt cgatgtatcc gcaggcttct tatcggcggg
     8401 gcggggcggg ggtgtgtctc ccggtgcttg caccggggct tttttcgctg gctttgctct
     8461 ctgcaagtag ctcttgagcg ttggggttgc gatgctcaga ccttcgccgc gcagcgtctc
     8521 ggaaatctgg tcaagcgtgt atccacgctt ttgcaaagcg gcaatctctt tggatagcac
     8581 gcgcacggcc tcttgcttgc tgtgctcctg cttcttcttt tcgactggcg gcatctcacg
     8641 aagctttgaa gcgatagcct ctagctgctc tgttgtgtat ttcattctgc taactccttt
     8701 gctcactggt tgcggagttg acagcatagc gccttttatg cttgttgcaa agtgcgtccc
     8761 gtgcacctaa actgctgata tgatagtata gtggttgagc aatacaggat gcggattgtc
     8821 atacacatgc tacgcatgtt cctgccaatc cccgcctgcc cattcggcgc ttcgcttctc
     8881 acggtctggc tcatcctgca aggggcaagc cccttgaccc cggacagtgc ggcgctttcg
     8941 cttcttactg tccaagacac accccgcccc cgcccccgcc cccgcccccg gcgagaagga
     9001 gacatgatgc catttgaaac gcaggggccg gaaccgctgg acgccgttat caatgtgcgg
     9061 ctgaccgcag cggagaaggc cagattgaaa gaggacgccg accttgccgg gttgagcatg
     9121 tccgagcttg tccgccgccg ctacttcggc aggccgatca ttgccaacgc cgacgccgtg
     9181 atgctcaagg agctgcggcg catcggtgga ttgctgaagc acatccacaa cgagagcgga
     9241 ggcgtttaca gcaaggaaac agccggggcg cttgttgcgc tcaaggacta cttcaggaag
     9301 ctaaacgatg atcgttaaga aggttccgaa tccgaagaag tccgcgagca aggcgcagcg
     9361 catcggccag cttaccagct atgtccgttc gccggagtcg gagagtccgc aggaaaagtg
     9421 tctgtatgcc ggggcgcggg gtttcatgat ggacgacccc aagagccaga cggcagagat
     9481 gattgcactc tcacaagaag ccgtgcgcag caaggacacc atcaatcact acgtcttgag
     9541 ttggcgcgaa ggcgaacagc caagcccgga gcaagtagag gaagcggtta gcatcttcat
     9601 ggatgagctt gggtggaaag accaccaagc catctatggc cttcattccg acacggacaa
     9661 tatccacctg cacatcgtca tcaatcgggt gcatcccgaa acgctcaaga ttgttgagaa
     9721 gaaccgtgga tttgatattg aactggcaca caaagccatt gcacggattg agcacgccca
     9781 aggctggcag cgtgagcaaa acgggcgcta tcaggtgttg gagaacgggg agctaggccg
     9841 agcgccatac gacccggaaa agccacgcca gcccgaccag aagaagcggg acatggagaa
     9901 ccgcaccggg gagaagtcag cccaccgcat cgccatcgag gacggcgcag ccatcatcaa
     9961 gcaggcgcag acgtgggagc agttgcaccg cgagttagca gccaagggga tgcgctacga
    10021 aaagaccggc agcggggcga ccgtgtttgt cggggatgtt ggcgtgaagg ccagcgatgt
    10081 agaccgcaac gccagccttg ccaagatgca gaagcggcta ggtgaatacc agccagcgcc
    10141 gcagcgtcag caggtagcgc cgcgcgagcc ggagccgatc aagccagacg tgccgggatg
    10201 gaaggactac atcaccgggc gcaaggcaca ctacgccgag aagaacgcgg acaagctggc
    10261 acaggacaag cggcaggagc aggagcgcaa gcaacttgcc gagcagcaga aggcccgccg
    10321 cgatgaactc atgcgcggaa actggaaagg caagggcgaa gtgctcaacg ccatgcgcag
    10381 cgtgattgcc gccgagcagg ccgcagagaa agcggccttg aaggagaagc accagaagga
    10441 gcgcgagcag caccggcagc ggttccgacc ctatcccgac ctggagcagt ggcagcggat
    10501 gcagaaaagc ccggagcttg ccgagcagtg gcgacaccgg gccagcgagc cgcagcgcat
    10561 cgagggcgac cgcagcgagc cgccgacgcc gcgagacatt cgcgcctatc agcctgaaat
    10621 cgtgggccag caggtgcatt acagccggaa ggaagaagcg gggcggggcg ggggtgtgtc
    10681 tttcgtggac aagggcaaaa gcatcgatat tcacgactgg cgcaaccggg acagcacgct
    10741 tgccgcgtta cagctatcgg cgcagaagtg gggcagcttc accgtgatgg ggaacgacga
    10801 atacaaggcc atgtgcggga agttagcagc agagcacggc ttcaagatca caaatccaga
    10861 gcttcaggag agcatccagc aggagcggca gcggatacag caggaaaggg tgcaggcgat
    10921 gaaatcggag cagctaaagc agttcgagcg gtacgcggaa gcggtaggcg cagagcgtta
    10981 ccgggtaacg tctatcaaga tgcgcgagga cggcagcaag caaaccttca tcctcgacaa
    11041 gaaggacagc atcacgcggg gcttcacacc gcaggagatc gagcagcgca cgccggagat
    11101 gcagcgccta cagcgccggg gcgaaaacct ctactacacg ccgctatcgg acaagaagca
    11161 tcacatcctc atcgacgaca tgaaccggga gaagctggag cggcttatcc gcgacggcta
    11221 ccagcccgcc gccgtgcttg aatccagccc cggcaactat caggccatca tcaccgtgcc
    11281 gaagctgggg acggcccacg ataaggacgt gggcaaccgg ctgagcgatg ccctgaaccg
    11341 tgaatacggc gacccgaagc tatcgggagc catccacccg caccgtgcac ccggctacga
    11401 gaaccgcaag cccaagcacc agcgggagga cggcagctat cccgaggtgc gcttgctcaa
    11461 ggccgagcgc cgggagtgca tcaaggcgct ggccttgtcc agccagatcg acgccgagta
    11521 tcagcggcaa gcggccttga aggcgcagca gcccgagcgc agcaaagcca agcccgcctt
    11581 ggagcttgca gcggccagcg gcagcgcgat cgacgcctac cagcggcatt atcgcgacgt
    11641 gctcaagcgg cagcgtggcg gcgaggtgga cttgtcccgc ttggattcca tgattgccgt
    11701 gcgtatgcgc gtcaccggcc acgatcaagc cgccatcgag ggcgctatcc gccagtgcgc
    11761 cccggccacc cggcagaagg acgagggccg cgactggaac gactacgcgc agcgcaccgc
    11821 ccgctatgcc tacagcgccg caggcgaccg ccaagccgcc gagcttggga agtaccgcca
    11881 gcagtgggag aagctggaag ggcgcgagcc tgtacgccag caggagcagg ccaaggcgca
    11941 gaagatcgag cgcgataact cgccggggat gagtctttaa atacctgttg catgtaactt
    12001 gcgaggtgcg tacaatgttg catgtaactt aattggaggc ttggaaatgg caacagcaca
    12061 cgttaatcag cgggttcaga agcatcgcga tgcattacgc atggcggggc ttcgtccggt
    12121 tcaaatctgg gtgccggata cgcgccgccc cgacttcgcg gaggagtgcc gccgccaatg
    12181 cctgcttgtg gctcaggccg acagcgccga caccgccatg cagcagttca tggatgaagc
    12241 cttggcagac gtggacggct ggacggaatg atgcgcggcg accttgtgac catcgccatg
    12301 cagggcgact ttggcaagcc acggcctgcg cttgtggttc aggccaacca gttcagcgag
    12361 cacggcagcg ttaccgtgct gcctgtcacc agcacactta atgccgcgcc gttgctgcgc
    12421 gtcacggttc agccgagcgc agagaacggt ttgcagaagc catcgcaggt gatggtcgac
    12481 aaggcgatga cggtgctgcg cagcaaggta gggccagctt tcgggcgcat cgacgcggac
    12541 gcactgcttg aggttgagcg ttgcttggcc gtgttcttgg ggattgcgaa atgaaaacga
    12601 tctacaccat tcccgccccg gattccttgg gcgcgatgat cgaggtctac ggcgagccgg
    12661 aaaacgcttg gtacgagtgg cgcatcatcg acggcgggcg caccgtgcgg gacaccggca
    12721 cggaaggcca cagcgccttt cagggacgcc agtacgggca agcagagatt gccctacgtg
    12781 atgcgctcat gttcgcttct ggcctgccca tcgacggcga cacgccgacc gatgccgcca
    12841 gtctggaagc gggctatgcc gccaaggcga aggcagaagc ggcgcggcag ggtgcgggaa
    12901 cacccggcag cgcctaacca taaccgttct acacaggaga acagc
//
