LOCUS       NZ_CP137441             5472 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain 2023CB-00436 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP137441
VERSION     NZ_CP137441.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37844966
            Assembly: GCF_032738105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 5472)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/09/2023 14:25:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,234
            CDSs (total)                      :: 5,109
            Genes (coding)                    :: 5,013
            CDSs (with protein)               :: 5,013
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 18 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137441.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023CB-00436"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            25..348
                     /locus_tag="R1Q26_RS26140"
                     /old_locus_tag="R1Q26_26140"
     CDS             25..348
                     /locus_tag="R1Q26_RS26140"
                     /old_locus_tag="R1Q26_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_318523644.1"
                     /translation="MLTLWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     APALLRQLAGMGNNLNQTARKVNSGAWSGHDRVQVVAALMAIDAGLERLRHAVLEKGRD
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            557..>988
                     /locus_tag="R1Q26_RS26145"
                     /old_locus_tag="R1Q26_26145"
                     /pseudo=""
     CDS             557..>988
                     /locus_tag="R1Q26_RS26145"
                     /old_locus_tag="R1Q26_26145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1026..1550
                     /locus_tag="R1Q26_RS26150"
                     /old_locus_tag="R1Q26_26150"
     CDS             1026..1550
                     /locus_tag="R1Q26_RS26150"
                     /old_locus_tag="R1Q26_26150"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_318523641.1"
                     /translation="MSSLSKMATDFRQKSNEQANDTEQLLKQEFDRLGTCVSEALSLSG
                     QKISDDIAARNRELSEALRHHSNGMTEAMQSHRESVMRLATRRWLSVLMTSLLLAGTCG
                     GVLWFLSWKIASNLDEIAAQNVTLEKLNARTWGVEFREGKDGRYLILPKGTEADTNWTA
                     EKRRAVKLVKE"
                     /name="MbeB family mobilization protein"
     gene            1557..1772
                     /locus_tag="R1Q26_RS26155"
                     /old_locus_tag="R1Q26_26155"
     CDS             1557..1772
                     /locus_tag="R1Q26_RS26155"
                     /old_locus_tag="R1Q26_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_318523642.1"
                     /translation="MTELETQLLSALEQLQQDYSQRLDEWESAFGELRTMYGLTQRDNA
                     RLSEAVSSLSQRVQGLSEQVRRLSRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1955..3829
                     /locus_tag="R1Q26_RS26160"
                     /old_locus_tag="R1Q26_26160"
     CDS             1955..3829
                     /locus_tag="R1Q26_RS26160"
                     /old_locus_tag="R1Q26_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="WP_318523643.1"
                     /translation="MATGFFLYHLDKTTCGGRILSGAEDDTYETGGIERQQVREGDPVT
                     CGKHPGRFRVCGGMGDTYEVGGVLKEWAGSLDSYSSCPCRAKFIPSVETHTYESDCNAG
                     RVAERERAAEKKAQQSEPEQRAQAAKKKREITLTIGVFFDGTGNNANNSGDRQAVCTAE
                     HYRMNDVDAGSAYSQCVRLKRGYSGTAAGSYLGYYSNVHWLNTLYSQGIRPDTGAGQYA
                     IYIEGIGTKDGAGDSTYGMGTGRGDTGVVRKTDRAVAALVAGIQGYFNSHTDARTCNIK
                     ELQFDIFGFSRGAAAARHFANRVFSQDSAIIAAIKTGLAGAEFSGTPGGKTRFLGIFDT
                     VAAIGTPVNGLNPHSADTGDVNLVLRPGVAEKVFHITAQHECRFNFALNSVKPAWPELA
                     LPGAHSDIGGGYNPDEHEAYFLTRPEFETVPLSTPDTETRIYRQTCAQLKAMDGYPTIA
                     PLLNAVAVNADTWHDDRLPADRYGTLQKRSGAALVIDRLTRNDWSKVVLRVMLDAAQDA
                     GVVFDPMDETAPDFALKADLNALCEKAIVMGRAARSGRSAPGFTTPEIRMLAEKYIHCS
                     ANWNSVVRDGQGMIRGAVKPAKLVTFTNRPDERWQRTVYDMDGNKIWK"
                     /name="DUF2235 domain-containing protein"
     gene            3820..4473
                     /locus_tag="R1Q26_RS26165"
                     /old_locus_tag="R1Q26_26165"
     CDS             3820..4473
                     /locus_tag="R1Q26_RS26165"
                     /old_locus_tag="R1Q26_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004164810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="WP_318523640.1"
                     /translation="MEIIRLASLIPVLVLTACHGTDQPNTSVKNDVPTEWKFNFFTPKA
                     LPALVTFAAILDADGKDYRFNTLNSTSALPGVIGEWNDKDRAPGGYWNHVDSPPKHIIF
                     CWDSIIDKKVYETHLTLSAAVVEKMRSPSGKRDPYNNTYDTVLIGLAPEGKVAVWLQGI
                     GFEPNYRVTPSVLKTVSGDKLDICRGITKHPNGYKYYGDTPEFIRGKVYPYGKW"
                     /name="DUF2931 family protein"
     gene            complement(4679..4853)
                     /locus_tag="R1Q26_RS26170"
                     /old_locus_tag="R1Q26_26170"
                     /pseudo=""
     CDS             complement(4679..4853)
                     /locus_tag="R1Q26_RS26170"
                     /old_locus_tag="R1Q26_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          5309..5386
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.25"
                     /coverage="93.0"
                     /evalue="8.26e-28"
     Typing          complement(4741..5226)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.542"
                     /coverage="93.0"
                     /evalue="8.33e-106"
     Typing          312..1105
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="95.0"
                     /evalue="2.83e-143"
ORIGIN
        1 tgattgtccg ggaaacgcac caaaatgctc accctgtggg tgacggaaga cgagcaccgg
       61 cggttactgg aacgctgcga cggtaaacag ctcgcagcgt ggatgcgcca gacgtgcctg
      121 gacgagaagc cggcacgcgc cggcaaactc ccatcgcttg cgccggcgct gctgcgtcag
      181 cttgccggga tggggaacaa cctcaaccag acagcccgga aggtgaacag cggggcgtgg
      241 tccgggcatg accgcgtgca ggtggtggcc gcgctgatgg ccatcgatgc cgggcttgaa
      301 cgtctgcgcc atgccgtgct ggaaaaaggg cgcgacgatg atagttaaat tccacgccag
      361 agggcgcggc ggcggctccg gcccggtgga ttacctgctg ggtaaggagc gtaaccggga
      421 gggcgccagc gtcctgcagg ggaagccgga agaggtccgg gagcttatcg acgcctcgcc
      481 ctatgccaag aaatacacgt ccggggtgct gtcattcgcg gaggcggact tgccgccggg
      541 gcagcgtgag aaggtaatgg agggcttcga gcgggttctg atgcccggac tggataaaga
      601 ccagtacagc atcctgtggg tggagcaccg ggacaagggg cggctggagc tgaatttcct
      661 gataccgaac accgaactgc tgaccgggaa gcgcctgcag ccgtattttg acagggccga
      721 ccgtccgcgc atcgatgcgt ggcagaccac agtgaacgcg aagctggggc tgcatgaccc
      781 taacgcgccg gagaaccgcc ggacgctggt cacgccgaac accctgccca gaacgaagca
      841 ggaggccgcc gaggcgatta cggacggtct ggtgcgactt tgtgtggccg gagagcttaa
      901 aacgcgtcag gacgtgatac aggcgcttac agcgtcagga tttgacgtgg tgcggaccac
      961 gaagaccagt atcagcctcg cggacccgga ggggggccgc aatctcagac tcaggggagc
     1021 aatctatgag cagtctttcg aaaatggcga cggacttcag gcagaaatcg aacgagcagg
     1081 cgaacgatac agagcaactg ctgaagcaag agttcgacag gctcgggacg tgtgtcagcg
     1141 aggcgctgag cttaagcggg cagaaaatca gcgacgacat agccgcccgg aaccgggagc
     1201 tgagcgaggc gctgaggcac cacagcaacg gtatgacaga ggccatgcag tcacaccggg
     1261 agagcgtgat gcgcctggcc acacgccggt ggctgtcggt gctcatgacc tcgctgctgc
     1321 tggccgggac gtgcgggggc gtgctgtggt tcctgagctg gaaaatagcg tcgaacctgg
     1381 acgaaatagc ggcgcagaac gtcacgctgg agaagctgaa cgcgcggacg tggggcgtgg
     1441 agttccggga ggggaaggac ggacgctacc tgatactgcc caaagggacg gaagcagaca
     1501 cgaactggac ggcagagaag cgccgggcgg tgaagctggt gaaggagtaa cggagcatga
     1561 cagagctgga aacccagttg ctgagcgcat tagagcagct gcagcaggac tactcgcaaa
     1621 ggctggacga gtgggagagc gccttcgggg aattgcggac gatgtatgga cttacgcaac
     1681 gggacaacgc gaggctgagc gaagccgtga gcagcttgag tcagcgggtg cagggcttaa
     1741 gcgagcaggt gaggcgcttg agccggtcgt gacgcggcac gagtacgagg tggcggcagc
     1801 gcatgcgcgg gtggtgcatg aacagcgcca gaaggagctg acaaaagagc gggagtatcc
     1861 ggggccatcg cttgagctgt agcggaaaat tagggttatg tcgtgccggg tgtatgatgc
     1921 gcggacttta taaccatttc attcatgagg gattatggca acgggatttt ttttatatca
     1981 cctggataaa accacctgcg gggggcgcat tctgtcgggc gcggaggatg atacctatga
     2041 aaccggtggc attgagcgcc agcaggtcag ggagggcgac ccggtgacct gcgggaagca
     2101 tccggggcgg ttccgggtgt gtggtggcat gggcgatacc tacgaggtgg gcggcgtgct
     2161 gaaagagtgg gcgggttcgc tggacagcta cagctcctgc ccgtgccgcg cgaagtttat
     2221 cccgtcagtc gagacgcaca cctatgagag cgactgcaac gccggtcggg tggccgagcg
     2281 tgagcgggcc gcggagaaaa aagcgcagca gtcagagcct gaacagcgtg cgcaggcggc
     2341 gaagaagaag cgggaaatta cgctgacgat cggggtgttc ttcgacggca ccggcaataa
     2401 tgcgaataac agcggcgatc ggcaggcagt ctgtactgcc gaacattaca ggatgaatga
     2461 tgttgatgca ggatctgcgt attcccagtg cgtgcgatta aaacggggtt acagcggcac
     2521 tgcagccgga agctatcttg gctactacag caacgtgcac tggctcaata cgctgtacag
     2581 ccaggggatc aggcccgata cgggagccgg tcagtatgcg atttatatcg aaggtatcgg
     2641 tacgaaagat ggcgcgggcg acagcactta cggaatgggc accggccggg gcgataccgg
     2701 cgtggtgaga aaaaccgaca gggcggttgc ggcgctggtt gccggtattc aggggtattt
     2761 caacagtcac accgatgccc gcacatgcaa catcaaagag ctgcagtttg atatttttgg
     2821 tttcagccgg ggagccgcag cggcgcgaca ctttgccaac cgggtattca gtcaggacag
     2881 cgcgattatt gcggcgatta agaccgggct ggctggcgct gaattttccg ggacgcccgg
     2941 cgggaaaacc cgctttctgg gaatatttga taccgtggcg gccatcggta cgccggtgaa
     3001 cgggcttaat ccgcacagtg cggacaccgg cgatgtgaat ctggtgctgc gtccgggtgt
     3061 ggccgagaag gtttttcata tcaccgcgca gcacgagtgc cggtttaatt ttgcgctgaa
     3121 cagcgtaaaa ccggcgtggc cggagctggc ccttcccggg gcgcactctg acattggcgg
     3181 cggctacaat cctgacgagc acgaagccta cttcctgacc cgtccggagt ttgaaaccgt
     3241 gccgctttcc accccggata cggaaacgcg aatttaccgg cagacctgcg cgcagcttaa
     3301 agcgatggat ggttatccaa ctatcgcgcc gctgctgaat gcggtggcgg tgaatgcgga
     3361 cacctggcat gacgacaggc tgcccgccga ccgctacggc acgctgcaaa agcgcagcgg
     3421 tgccgcgctg gtgattgatc gtctcacgcg caacgactgg tccaaggtag tgctgcgggt
     3481 gatgctcgat gccgcgcagg atgcgggcgt agtattcgat cccatggatg aaaccgcccc
     3541 ggattttgcg ttaaaagccg atctgaatgc tttatgtgaa aaagcgatag tgatgggccg
     3601 tgccgcccgc agcggccggt ctgcccctgg ttttaccacc ccggaaatcc ggatgctggc
     3661 cgaaaagtat attcactgtt cggccaactg gaacagcgtg gtgagggacg gtcaggggat
     3721 gatccgcgga gcggtcaaac cggcgaagct ggtgacgttc accaaccggc cggacgagcg
     3781 ctggcagcgg acggtctacg atatggacgg gaacaaaata tggaaataat tcgtctggcc
     3841 agtctgatcc cggtgcttgt gctgacggcc tgccacggaa ctgaccagcc caatacctca
     3901 gtcaaaaatg atgttccgac ggagtggaaa tttaactttt ttacgccaaa agcattgcca
     3961 gcactggtga cattcgctgc tattcttgat gcagatggga aggactatcg ttttaacacg
     4021 ctgaacagca catcagcgtt acccggtgtt attggtgagt ggaatgacaa agatcgcgca
     4081 ccagggggct actggaatca cgttgatagc ccaccaaaac atattatttt ttgctgggat
     4141 tccataattg ataaaaaggt gtatgaaacg catctcactt tgtccgctgc tgttgtggaa
     4201 aagatgcgca gtcccagcgg gaaaagagat ccgtacaata acacctatga cacagtgcta
     4261 attggtctgg ctcctgaagg caaagtggct gtctggcttc aggggatcgg gtttgagcct
     4321 aattatcgtg tcaccccatc agtcctaaaa accgtatcag gggataaatt agatatttgc
     4381 cgaggaataa ctaaacatcc gaatggatat aaatattacg gggacacgcc tgagtttatt
     4441 cgggggaaag tatatccata tgggaaatgg taatgttttt ccgcttttgg gacgcgaatg
     4501 atttttttct gaaaaaagga tcttcttgag atcctttttt tctgcgcgta atctcctgcc
     4561 ctgtaaacaa aaaaaccacc gctaccaacg gtggtttgtt tgccggagtt cagagctacc
     4621 aactcttaat tccgaaggta actgggtata cgagaccaca gataccaaaa ctgttctttc
     4681 agtgtagccg cagtaaggcc catacttcaa gacctctaaa tatctctctc gtataccccc
     4741 agttaccagt ggctgctgcc agtggcgtat ttacgtgtcc gtccgggttg gactcaagac
     4801 gatagttacc ggatgagggc gcagcagtcg ggctgaacgg ggggttcttg cacacagccc
     4861 agcttggagc gaactgtcta aacggaaccg ggtgtcaggc gtggtatgag aaaacgcggc
     4921 ctttaacagc ggaaagacac cggtagaccg catgacggga acaggagagc gcaagaggga
     4981 gccgccggag agaaatcacc ggcatcttta agtcctgtcg ggtttcgtcc tcctctgact
     5041 tgcagcgtcc atttttgtga tgctcgtcag gggaggcgga gcctatggaa aaacggctcc
     5101 gccgtatcct tttcccggcc cttcctgttc tgagcgctcc cgtttaatcc cgttatccgt
     5161 gagccggtaa cgcccgccgc agcctcatga ccgaacgtag tgagcgaatg agcgaggagg
     5221 cgtcatttca tctgtgcgta cagagtactg cgatcactcc tgagccacat atcacatctg
     5281 tcacatgaag cacttccctg aaaccttact ctgccttaac atattaagcc agtatacact
     5341 ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgctaac accgctgacg
     5401 cgcctgcggc ttgtccaacc ccgcgccgac cgggaaataa tttcccgccc gtcccggcgt
     5461 tatcaggagg cc
//
