LOCUS       NZ_CP137440             5818 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain 2023CB-00436 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP137440
VERSION     NZ_CP137440.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37844966
            Assembly: GCF_032738105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 5818)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/09/2023 14:25:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,234
            CDSs (total)                      :: 5,109
            Genes (coding)                    :: 5,013
            CDSs (with protein)               :: 5,013
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 18 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137440.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023CB-00436"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(482..691)
                     /locus_tag="R1Q26_RS26105"
                     /old_locus_tag="R1Q26_26105"
     CDS             complement(482..691)
                     /locus_tag="R1Q26_RS26105"
                     /old_locus_tag="R1Q26_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            1291..3588
                     /gene="kba"
                     /locus_tag="R1Q26_RS26110"
                     /old_locus_tag="R1Q26_26110"
     CDS             1291..3588
                     /gene="kba"
                     /locus_tag="R1Q26_RS26110"
                     /old_locus_tag="R1Q26_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease bacteriocin klebicin B"
                     /protein_id="WP_108451945.1"
                     /translation="MGGGFNYNGEGATGTGLDRDPYVRDSNGNAIGVKSGYHAESYGTS
                     SPALGPNGAIQITAGVIAVPGDKPRPDGGSGGGNTVNTGPAGQLLVMNKGQLGYWETRS
                     TGAGNNEHNTSVFVAVGPSEAEKTASAEKALKEKQQAEAAAKDFAAKTAAASATAEKER
                     QQAIAAATAAGQHQSVSDARNSLNNATSDVSRLKSAADSALQEAKAKRKAAIDAVPVAT
                     QAENKYQELQQKIKGLKLKNGEYGTDKWEIIGSNKEHDHWGYRFYPSGITKAQVDAAQS
                     DAVNKRNQATSLASQATAAEQDSLKATAAYNAAETRRQAAQAALNSAEQAAAAERKRQE
                     AEAAAAAAAEKKRQADAAAKAAEEARAAAEKARLMQERQAAADKLKSTDIQSVRGIPST
                     AAPAASPISWAVASLGGISLDSVTAGKAWTQIAEVMAKLRGIAGASLVGPVVATAVGLF
                     WSRDVGIGSDVVPGRDISGLMPGDALSLPDLATLIKAADSKTGVSMPVRGRIIVREGDY
                     LESQFVRTPVAGSVPVVRAALDKATGYWGYTLPAIQGVPGQTILVSPSDAPGVNGPLGL
                     AGPVPLPETIIHTGGQTTVPQGGTVTVSPAEDDIDFNDLILVFPPESGLKPLYVMYRSP
                     RNMPGTASGKGQNVGNNWMGGTSTGDGAPVPSQIADKLRGKAFGSFDSFRRAFWKAVAA
                     DPDLSKQFYPDDIERMKLGRAPTVRFRDSVGKRVKVELHHKVEISKGGDVYNVDNLNAL
                     TPKRHIEIHKGN"
                     /name="kba"
     gene            3590..3847
                     /locus_tag="R1Q26_RS26115"
                     /old_locus_tag="R1Q26_26115"
     CDS             3590..3847
                     /locus_tag="R1Q26_RS26115"
                     /old_locus_tag="R1Q26_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015366249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_010904244.1"
                     /translation="MANKTLADYTEQEFIEFIEKIKKADFATESEHDEAIYEFSQLTEH
                     PDGWDLIYHPQAGADNSPAGVVKTVKEWRAANGKPGFKKS"
                     /name="bacteriocin immunity protein"
     gene            3955..4110
                     /locus_tag="R1Q26_RS26120"
                     /old_locus_tag="R1Q26_26120"
     CDS             3955..4110
                     /locus_tag="R1Q26_RS26120"
                     /old_locus_tag="R1Q26_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_200557628.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVSLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(4315..4428)
                     /locus_tag="R1Q26_RS26125"
                     /old_locus_tag="R1Q26_26125"
     CDS             complement(4315..4428)
                     /locus_tag="R1Q26_RS26125"
                     /old_locus_tag="R1Q26_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049026061.1"
                     /translation="MHEPPVQSDCCALSGNCHLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            4844..5035
                     /locus_tag="R1Q26_RS26130"
                     /old_locus_tag="R1Q26_26130"
     CDS             4844..5035
                     /locus_tag="R1Q26_RS26130"
                     /old_locus_tag="R1Q26_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_087806444.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKFHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5035..5457
                     /locus_tag="R1Q26_RS26135"
                     /old_locus_tag="R1Q26_26135"
     CDS             5035..5457
                     /locus_tag="R1Q26_RS26135"
                     /old_locus_tag="R1Q26_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_200557627.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVTYDVKSDAESTAINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(4316..4723)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.917"
                     /coverage="99.0"
                     /evalue="2.74e-174"
ORIGIN
        1 aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg attgtcggcc
       61 gtcgacgaac ccgattcctg atcgtgctga aaaccggttt tccggctgcg ccggcaacgc
      121 ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatagga agaacgccgg
      181 aaacaagact taccctggct gaatggcgtg aattttcgcg ctatgcactt gcgtgcatac
      241 tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg gggtttcggg tggtttgttg
      301 cctgttttac cggtttcccg tcggaaacgc cctgaggtcg ttttagcggt gcgtacaatc
      361 atgcctttat gttaaatgac agatgcaaaa aagcccccga agggggctga aggtttttct
      421 gctaagggac gaaacgggcc gcctcccggc gacctgttcg gcagctccct ctgcgaccat
      481 cttatttggt cagttcagcc gtcatgtgga cacggttatt gaactgagcg ctggtgattt
      541 tgtatgacgc accttgttca gcggcctgtg cggcaatttt tgcttcagca cgatccaggg
      601 ttgaggcggt agcgctgacg ctctgagcaa aggcaccgaa agacatcatg gatagagcgg
      661 caactgcaac aaaagttttg atggatttca tggtcgtatt ccttaagtca ttttgttctg
      721 tatggggctt tgttgccctg atgtgataaa tgatagtacg accatgagct gattaaaatc
      781 gaaacaattt gcggatatca ttcaaaaaaa tagaacaaaa gttgaacaca tcaaaaccag
      841 gtgtgagact ctgatttagg ggcaggatga atagcctcga gggttcgtaa aattaaggga
      901 ttatggtaaa tgggagcgcc cgggtggcgg gataccctca acaccaagac tttttgtaca
      961 ggtctctgtc cgtcagtcac acctttttta agagcacata tcgttaattt tttttctctg
     1021 ccgagccgcg tcctgtctgg gttttttcat ttctctcatg attaacttaa tgaaaattaa
     1081 aaagataatt gtatctctcc gcgtgttgac agagttctca tgtcaggagt agtatccgat
     1141 tactgtatat aaacacatgt gtttatatac agtactttgg tgtggcagag aactaataac
     1201 agcctccgga tacaggttcg aaggcgataa accgcacacc agattctgac aagcaggaaa
     1261 acgataaaaa taacaacaaa ggagagagtt atgggtggtg gatttaacta taacggggaa
     1321 ggtgctactg gcaccggatt ggatcgtgat ccatatgttc gcgacagcaa tggtaatgct
     1381 attggtgtta aatcaggcta tcacgcggag tcctatggga cgtcaagtcc agccttaggg
     1441 cctaatggcg ctattcagat tactgctggg gttattgctg tgcctggaga taagccccga
     1501 cctgacggtg gtagtggtgg tgggaatact gttaacaccg gacctgcagg acagctcctg
     1561 gtgatgaata aaggtcagct tggatactgg gaaactcgtt ctacgggcgc gggtaacaat
     1621 gagcataata cgagtgtatt tgttgctgta ggtccttctg aggcagaaaa aactgcttct
     1681 gcagagaagg cgttaaagga aaaacagcag gcagaagcag cagcaaaaga ctttgcggct
     1741 aaaactgctg ccgcatcagc cacggcggaa aaagaacgcc agcaggcaat tgctgctgca
     1801 actgcagcgg gtcagcatca gtcagtttct gatgcccgta atagcctgaa taacgcgacg
     1861 tcagatgtgt ctcgtctgaa atcagctgca gacagtgcac tgcaggaggc aaaggcaaaa
     1921 cggaaggctg ctattgatgc tgtacctgtt gcaacccagg cggaaaataa atatcaggag
     1981 ctgcagcaga agattaaagg cctgaagctg aaaaatggtg agtatggtac ggataaatgg
     2041 gaaataattg gctctaataa ggagcatgat cactggggat acaggtttta tccatccgga
     2101 attaccaaag ctcaggttga tgcggcgcag agcgatgctg tgaataagcg aaatcaggct
     2161 accagtcttg ccagtcaggc aacagcagca gaacaggaca gcctgaaagc tacagctgcc
     2221 tataatgcgg cagaaacgcg ccgtcaggct gctcaggcgg cgttaaattc tgctgaacag
     2281 gctgctgctg ctgaacgtaa gcggcaggaa gctgaggcgg ctgcagcagc agctgctgag
     2341 aaaaaacgtc aggcagacgc agcagcaaaa gctgcagagg aagcgcgtgc ggcagcggaa
     2401 aaagccaggc tgatgcagga gcgtcaggca gcagcagata agctgaaatc cacagatatt
     2461 cagtctgttc gcgggatccc gtctacggct gcgcctgcag cgtcacccat ttcctgggcc
     2521 gttgcatcac ttggtggtat atcgctggat agtgttactg cagggaaagc ctggacgcag
     2581 attgctgagg tgatggctaa actacgaggt attgccggtg cgagtcttgt tggtcccgtg
     2641 gtggcaactg ctgtagggct gttttggtca cgtgatgttg gtattggcag tgatgtggtg
     2701 cccggacgtg acatcagcgg gctgatgccg ggtgatgcac tgtcattacc tgatctggcc
     2761 actttgatta aagctgctga cagtaaaacg ggtgtcagta tgccggttcg aggccggatt
     2821 atcgtgcgtg aaggcgatta tctggagtct cagttcgttc gaacacctgt tgccggtagt
     2881 gttccggttg ttcgggctgc tctggataaa gctactggtt actggggata tacgttgccg
     2941 gcgatacagg gtgtgcccgg acagacaata ctggtgagtc cgtcagatgc gccgggcgtt
     3001 aatggtcctc tgggacttgc tgggccggtt cctttgcctg aaactattat acataccggt
     3061 gggcaaacta cggttcctca gggggggact gtgacagttt cgccggcaga agacgatatt
     3121 gatttcaatg atttaattct ggtatttccg ccggagtccg gtcttaaacc gttgtatgtg
     3181 atgtaccgta gccctcgtaa catgccgggg acagccagtg gtaaaggtca gaacgttgga
     3241 aataactgga tgggggggac cagtaccggg gatggtgctc ctgttccttc ccagattgca
     3301 gataaattac gtgggaaggc tttcggtagt tttgattctt tccgtcgggc tttctggaaa
     3361 gcggttgctg ctgatccgga cctcagtaag cagttttatc ctgatgatat agagcgaatg
     3421 aaattagggc gagctccaac agttcgattc cgagattctg taggtaaaag ggttaaggtt
     3481 gaactacacc ataaagttga aatttctaaa ggtggtgatg tctataacgt agataacctg
     3541 aatgcattaa cacctaaacg tcatattgaa attcataagg ggaactgaaa tggctaataa
     3601 aactttggct gactatacag agcaggaatt tattgagttt atcgaaaaaa ttaaaaaggc
     3661 agactttgct actgagtctg agcatgatga ggctatttat gagttcagcc agttgactga
     3721 gcatccagat ggttgggatc ttatttatca tcctcaagca ggagccgata actctcctgc
     3781 tggtgttgta aaaacagtaa aagagtggcg agcagctaac ggtaagccag gttttaaaaa
     3841 atcgtgattg aatgtgcggg ttcaagtgat gaatccgcac atttttaact ctggatttga
     3901 ataataatta ctagatatcc atttgcctag aataataaaa ataaaaggtg atatatgaaa
     3961 aaattacttt tcctgggggt tgttaccgtt tctgtggttc tggcggcatg tcaggtcaat
     4021 aatgttcgtg acaccggagg tggggctgtt tcaccttcat cgacggtgac cggagtctcg
     4081 cttgatagcg gtacgaacgg caatccgtaa aggatcttct tgagatccca tttggatcgt
     4141 cgtaatctct tgctctgtaa acgaaaaaac cgccttggcg ggcggttttt tcgaaggttc
     4201 gaggagttgg cgctctttga accgaggtaa ctggcttgga ggagcgcagt aaccaaattc
     4261 gttctttcag tttagcctta actggcacat aacttcaaga ctaactcctc taaatcagtt
     4321 accagtggct gctgccagtg gcgcttttgc atgcctttcc gggttggact caagatgaca
     4381 gttaccggat aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt
     4441 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     4501 acagcggaat gacaccggta aaccgaatgg caggaacagg agagcgcacg agggagccat
     4561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     4621 gtcaaattct gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc cgctgggcgg
     4681 cctcctcttt tccgcctccc ttgctcgctc ggttttctcg agcttttata agaacggtct
     4741 tgccgctcgc cgcagccgaa cgaccggagc gtagcgactg agtgagcgag gaagcggaaa
     4801 agagactgtt cttacactga gcactgacgc tctgaggctt cttatgacga aacaacagaa
     4861 aacagcgctg aacatggcta aattcatcca gaatcagtct ctgctcctgc tggagaagct
     4921 taacgagctt gatctcgatg tagaggccga cctttgtgag aaattccacg atgatgcgga
     4981 gcatcttttc cgtacactgt cctcgagact ggatgaatta caggatggta actgatggct
     5041 agagtgtcta ttagtgaagc ggcacggctt gttaaggtta gtcgtcctac aatttacaag
     5101 atgattaaca gtgggaagtt aagttacacc tctgttgtaa agcacgggaa ggctattaaa
     5161 gttattgata cttctgagct tattagagtg tttggttctc ttgatggtgt aatcgatact
     5221 gtaacatatg atgtaaaaag tgacgcagaa tctacagcta ttaacagcgt tggtttacat
     5281 gatttacaac atagaatcgc tttacttgaa gctgaaaacg atggtcttaa aggggctgtt
     5341 aaggctcgag atgagcatat tgattccctt cgtcaggcta tgcagcttct ggagcacaag
     5401 cacgaacctt cttcaccacc ccattccccg tggtggaaat tctggaaaaa atcctgaccc
     5461 cgtcatgctt cagttttcct ctccggccat cccggggagt tcctgaacct tcccaaaaat
     5521 cgtcagatct ctgaacgctc ctcaccacta caaataccca caccccacgc aaaattaaaa
     5581 ttttgaagat aactcattaa tatcagttag ttgtgggtta ttacattacg cagtattact
     5641 ttctgtaagg tgaatccaag tcgtcggcga ttcgtgtacg acttgccgac gactcgtcat
     5701 agagtcatcg acgactcact taagactatc agtaacttat gacgacttga ctctagagtc
     5761 gaattgtcat tttggtgttt ttttgacctt gacgactcta gagtcaagtc acgagtcg
//
