LOCUS       NZ_CP137439             7582 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain 2023CB-00436 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP137439
VERSION     NZ_CP137439.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37844966
            Assembly: GCF_032738105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 7582)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/09/2023 14:25:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,234
            CDSs (total)                      :: 5,109
            Genes (coding)                    :: 5,013
            CDSs (with protein)               :: 5,013
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 50 of 96
            Pseudo Genes (internal stop)      :: 18 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137439.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023CB-00436"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            join(5704..7582,1..140)
                     /locus_tag="R1Q26_RS26060"
                     /old_locus_tag="R1Q26_26060"
     CDS             join(5704..7582,1..140)
                     /locus_tag="R1Q26_RS26060"
                     /old_locus_tag="R1Q26_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="WP_318523639.1"
                     /translation="MHNGQMGYWENRSSGAGNNEHTTRAFVAVGPSEAEKAARAEKVAK
                     EKQQAEEAAKAFAAKTAAASAAAEKERQNAISAAAAAGQHQTVPDARNNLNQATAEASR
                     LKTVADNALNTAKNKRKEAIDAVPVATQAEKKYQDLQQSIKGLTLNNNGQYGTQKWEVI
                     SSNKEHDHWGYRFYPSGITKAQVDAAQNDAVNKRNAATSLASQATAAEQASLQASAAYN
                     AAETRRQAAQAALASAEQAAAAERKRQEAEAAAAAAAEKKRQADAAAKAAEEARAIAEK
                     AKALQARRTAADKLKSSEIQAVRGIPATAAPFAIPLTWSTASRGGFTLAADAAASLGAF
                     ISEALATLRVAVVANPVALTIAGLVLSKSVGVGSDMVPGRDISSMMPGDAFGLPDTAAL
                     NKAADQKTSVSMPVRGRLVMNDSGILDVQLVKTNTAGAVKVARAVLDKETGYWGYTLPA
                     VADVPAQTIFVSPADAPGANGPLTLSGPVPLPERILHTGDQISAPQATDKTVTPVADDL
                     DFDDIILVFPPESGLKPLYVMYRSPRNMPGTVSGKGQNVGNNWMGGASTGDGAPVPSQI
                     ADKLRGKTFGSFDSFRRAFWKAVADDSALSKQFSEADINQMKAGRAPTADFLESVGKRV
                     KIELHHEKEISQGGAVMDVDNIKALTPKNHIETHKGK"
                     /name="S-type pyocin domain-containing protein"
     gene            142..399
                     /locus_tag="R1Q26_RS26065"
                     /old_locus_tag="R1Q26_26065"
     CDS             142..399
                     /locus_tag="R1Q26_RS26065"
                     /old_locus_tag="R1Q26_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            507..662
                     /locus_tag="R1Q26_RS26070"
                     /old_locus_tag="R1Q26_26070"
     CDS             507..662
                     /locus_tag="R1Q26_RS26070"
                     /old_locus_tag="R1Q26_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(820..981)
                     /locus_tag="R1Q26_RS26075"
                     /old_locus_tag="R1Q26_26075"
     CDS             complement(820..981)
                     /locus_tag="R1Q26_RS26075"
                     /old_locus_tag="R1Q26_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_115659016.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHAKAPLAAATGNGFRGVSLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     gene            1398..1589
                     /locus_tag="R1Q26_RS26080"
                     /old_locus_tag="R1Q26_26080"
     CDS             1398..1589
                     /locus_tag="R1Q26_RS26080"
                     /old_locus_tag="R1Q26_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_110236262.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHIFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1589..1990
                     /locus_tag="R1Q26_RS26085"
                     /old_locus_tag="R1Q26_26085"
     CDS             1589..1990
                     /locus_tag="R1Q26_RS26085"
                     /old_locus_tag="R1Q26_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_285201244.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGNLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASAGQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="DNA-binding protein"
     gene            complement(2743..3273)
                     /locus_tag="R1Q26_RS26090"
                     /old_locus_tag="R1Q26_26090"
     CDS             complement(2743..3273)
                     /locus_tag="R1Q26_RS26090"
                     /old_locus_tag="R1Q26_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_285201243.1"
                     /translation="MKERKEAQRKITEDYSPELARQKVTSFFESHPKVAKSTNELSDTK
                     VCVLAALLDGKSITTSGYSIPSDYGVKRASAVAHALEKIHNFPISHSLVDTESDVGKMT
                     KQARFHMTESDREKLQRDPDCVFKEMYAKACKTRETQAQKEMKRLLKEYGEDGVLELLK
                     RAANDESGSKDAV"
                     /name="hypothetical protein"
     gene            3877..4419
                     /locus_tag="R1Q26_RS26095"
                     /old_locus_tag="R1Q26_26095"
     CDS             3877..4419
                     /locus_tag="R1Q26_RS26095"
                     /old_locus_tag="R1Q26_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="WP_285201242.1"
                     /translation="MSIDEVAQWHPFTDPDGNIWDLSFLDAHEVVYTHSAPGKSDIIYK
                     FIVSYSFHCFCKDYPEQSMEEKSALMYYAGKDARPFCRIRYTLAREKLRAIIEGLGNAK
                     VIHAGYGSYAFVEITVNDGQKEFYFVPFKAYRERKKLRLHVTSAYPVNERPTGKPVSFF
                     KIAHNLLRGKPLPKPQM"
                     /name="stationary phase growth adaptation protein"
     gene            5399..5791
                     /locus_tag="R1Q26_RS26100"
                     /old_locus_tag="R1Q26_26100"
     CDS             5399..5791
                     /locus_tag="R1Q26_RS26100"
                     /old_locus_tag="R1Q26_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_318523638.1"
                     /translation="MDLIITVRVLRAQALAPLLPVMWLISLIVLVGRKSGHQMSRIFAV
                     MVMVGIWRIFGGRFTLCPLRKAQQLQQLSVASMVSAVLLPVMAEVRVGRGHRDSLSLCI
                     TGRWDTGKTDHPGLVITNILPALLWQ"
                     /name="hypothetical protein"
     Typing          complement(869..1277)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="99.0"
                     /evalue="2.74e-174"
ORIGIN
        1 ttttagaatc agtagggaaa cgtgttaaaa ttgaattaca ccacgaaaaa gaaatatctc
       61 aaggaggggc tgttatggat gtcgataaca ttaaggcgct aacacctaaa aatcatattg
      121 agacacataa aggaaaataa aatggctggt aataaaattt cagattttac ggaagaagaa
      181 tttactgctt ttgttagtaa aataatcgct gctgattttc caactgacaa agagaacgat
      241 gatgctattt attcgttcgc ccaactaaca gagcatcctg ctggttggaa cctcatcttc
      301 aagcctaagg taggcgaaga ttcttcggcg aaaggaattg ttaaaaccgt aaaggaatgg
      361 cgagctgcta acggtaagcc aggttttaaa aaagcgtgat tgaatgtgcg ggttcaagtt
      421 atgagcccgc tcatttgtaa ctctgaattt gaagaataat tactagatat ccatttgcct
      481 agaataataa aaataaaagg tgatatatga aaaaattact tttcctaggg gttgttaccg
      541 tttctgtggt tctggcagca tgtcaggtca ataatgttcg tgacaccgga ggtggggctg
      601 tttcaccttc atcgacggtg accggagtca cgcttgatag cggtacgaac ggcaatccgt
      661 aaaggatctt cttgagatcc catttggatc gtcgtaatct cttgctctgt aaacgaaaaa
      721 accgccttgc agggcggttt ttccgaaggt tcaaacactt gcaggtgatt gaaccaaggt
      781 aactggcttg gaggagcgca gtcaccaaaa cttgttcttt cagtttagcc ttaacaggcg
      841 catgacttca agactaactc ctctaaatcc gttaccagtg gctgctgcca gtggcgcttt
      901 tgcatgtctt tccgggttgg actcaagatg atagttaccg gataagacgc agcagtcgga
      961 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttcc cggaactgag
     1021 tgtcaggcgt ggaatgagac aaacgcggcc ataaacagcg gaatgacacc ggtaaaccga
     1081 atggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg gtatctttat
     1141 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttccgtgatg ctcgtcaggg
     1201 gggcggagcc tatggaaaaa cggccgcgct gcggccttcc cttttttcca tcatgcgata
     1261 gcttggttct ctcgagcttt tctacgcatg ctcctgccgc tcgccgcagc cgaacggcgg
     1321 gagcgtagcg actgagtgag cgaggaagcg gaaaagagaa catccctata ctgagcactg
     1381 acgctctgag gcctcttatg acgaaacaac agaaaacagc gctgaacatg gctaaattca
     1441 tccagaatca gtctctgctc ttgctggaga agcttaacga gcttgatctc gatgttgagg
     1501 ccgacctttg tgagaaactc cacgatgatg cggagcatat tttccgtaca ctgtcctcga
     1561 gactggatgc attacaggat ggtaactgat ggccagagtg tctattagtg aggctgcccg
     1621 actgacgggt aagagtagaa caacactgca caggttaatt aaagcaggta acctgtccac
     1681 ctgtagcggt gaacgtaatg caaaaatggt agatacttca gagcttttac gtgtttttgg
     1741 tccctttgag caaccgaagg gtgaacacca ttctggacag gtctctgaac agcgtgatac
     1801 agcctctgct ggccagagtg aacaggtgat tcgtcatctg aaacaggaga ttgaacatct
     1861 gaacacctta cttttgtcca aggattcgca cattgacagc ctgaaacagg ccatgctcct
     1921 tcttgaaaat caccaggcaa aattaactga atccccggcg ccgtggtgga aattctggaa
     1981 aaaatcctga tcccgatgct tcagttttcc tctccggcga taccggggag ctcctgaacc
     2041 ttcccaaaaa tcgtcagatc tctgaacgct cttcaccacg aaaaataccc acaccccacg
     2101 caaaattaaa attttgcaga tttgtattta atatcagcta cttacgttca gtatatttcg
     2161 ttttttttat tttatttaca tagatccgac tctatgacga ctcgccgggg actctatgac
     2221 gagtcgtcat agagtcggta acgactcagg actataaaaa caatgagtta ataacgaccc
     2281 gactctagac acgactcccc gtttggttgt tttttatccg cgacgactct aggcacgact
     2341 ctaacgtaga gtcgtgccta gagtcgtcag gtgtctcgcc ataacgtgat tgctggctgt
     2401 tgacgaaccg gattcttcta cgtgctagaa aggctattcc ggcgctgccg gcaacccccg
     2461 ttctcggcac agtaaactgc gccgagaacg ggggttacat aggaaaagaa atccgatcat
     2521 ccgttagtct tagtaatgcg gtacggcatg cgccaattaa atgagaaagc tgattagtaa
     2581 ctgaaaaata gtattgatga atgtctgaat gcaattaaat aaaagacagc aaggaaacaa
     2641 accaaaccga gtcttgaaac cgaactggct tggtttgttt cttaattgta ttcgtcaggt
     2701 ctagtggcgt tatgaaaaag gcctacctga ggccttttat tgtcatactg cgtcttttga
     2761 gcctgactca tcgtttgcag ctctcttaag taactcaaga acgccgtctt ctccatattc
     2821 tttaagtaag cgcttcattt ccttctgcgc ctgtgtttcc ctagtcttgc aggcttttgc
     2881 atacatttct ttgaatacgc aatccggatc cctttgaagt ttctctcgat cgctttcggt
     2941 catatgaaag cgtgcttgtt ttgtcatttt tcccacatct gactctgtgt ctactaggct
     3001 gtgtgatata gggaaattat ggattttctc taacgcatga gcgacagctg atgctcgttt
     3061 tacgccgtag tcagaaggta tagagtaacc ggatgttgtg atcgattttc cgtcgagaag
     3121 tgctgccagt acacaaactt ttgtgtcgct cagttcgttt gtactttttg ctacctttgg
     3181 atgggattcg aagaatgagg tgactttctg gcgagctaac tccggagagt aatcctcggt
     3241 gatcttgcgt tgtgcttctt tgcgttcttt catctgatat accttttgat aatcaagata
     3301 aaattggctc agaaactgaa gtcggtcctt tacagcacag gacctttgca caaaatgctg
     3361 gaaatttttt tatcatttcc gtaataatgc gccacggcct aagatggttt gcaatgcatc
     3421 caacttaggt cgcttcagtt tctgaactgg agagcagcag ctatcaggtt tttcggttcc
     3481 gaggccattc acctgatagc tgcttttttt atttctgacg cccgtgatca cacggtaacc
     3541 agagatgtga ttgtacccga gttactgctt cgatacataa cattattgct tagtgctgtc
     3601 ttttttatgt cctacagtgc cgccagctag attgtgaggg gtttttgccg aatttatgca
     3661 atatacctca gagtataaca ataactcgcc acagggccgt tcaggcccaa atttgcagta
     3721 tgttgaaata tacagcacta tagttttgac tacatatgat ttagcatggt gatggctgca
     3781 gtacaatgtt gctacgcttt tgagcatttc ctcctgctcg tatggtgtct gatagaatac
     3841 atgggttctc ttggcttatc gtgttgaggt tttgctgtga gcattgatga agttgcccag
     3901 tggcatcctt ttacggatcc cgatggcaat atatgggatt tatcatttct tgatgcacat
     3961 gaggttgttt acacccatag tgctcctggc aaatcggata tcatttacaa gtttatcgta
     4021 tcttattcat ttcattgttt ttgcaaggat tatcctgagc aatcgatgga agaaaagtcg
     4081 gcattgatgt attacgcagg gaaagatgcg aggccgtttt gcaggattcg atataccctt
     4141 gccagagaaa agctacgagc catcattgaa gggctgggta atgctaaggt tattcatgca
     4201 ggctatggta gttatgcttt tgtagagatt acggtaaatg atgggcaaaa agaattctac
     4261 tttgtaccgt ttaaggctta tcgtgaaagg aagaagctac gtctgcatgt gaccagtgct
     4321 taccctgtaa atgagcgacc gacaggtaag ccagtgagtt tcttcaagat cgcccataat
     4381 cttttgcgtg gcaagccttt acccaaaccc cagatgtaac aaagcccgct ttgtcgcggg
     4441 cttcgccaga aatttcttct ggtattcaga acacctttac cgggcttggc ctcgaccaat
     4501 attgctattg gtagtgggat gtctggcata accagtctgc tgcatcctta aaataattct
     4561 atacgcatta ccgcaggtat tcaacaaaag gttgagcact cctaaaagct aatctttcgc
     4621 aggctgtctt tattttattg atttatatat gttttatgat ttacagctag gtcttgccta
     4681 aatgtgcaga ctcaatcgcg taaaccactt gaactacatt gcttctacat ggcgatgagg
     4741 gaatttggtg tttgaggtat ttgattagca tgtggtctgt cccacgtctt atgggcagga
     4801 aaaaccgata gtaaactgta aaaaattaat atctgagctt tagatcgtcg tctaactgac
     4861 agttagtcat ttttctgcat cgctatgcat aatcgtcaaa ttaacctagc gcgttttgac
     4921 ggtttttttg tgaggatgtt gcctgtttta ccggtttccc gtcagaaacg ccctgaggcc
     4981 gttttggcgg tgcgtacaat taagggatta tggtaaatgg gggcgctcgg gtggcggaat
     5041 accctctaca ccaagacttt tggtacaggt ctctgtccgt cagtcacacc ctttttaaga
     5101 gcacatatcg ttaatttttt ttctctgccg agccgcgtcc tgtctgggtt ttgtcatttc
     5161 tattacgctt aacttaatga aaattcaaaa gataattgta tctatccgcg tgttgacaga
     5221 gttctcatgt caggagtagt atccaagtac tgtatataaa cacatgtatt tatatacagt
     5281 acttggcgtg gcagagaact aattacagcc tccggatagc tgtcctgaag caataaaccg
     5341 cacgccgtac ttcagaaaag tggaaaacaa aaaaacaaaa gaaggaaatg tgtgatgggt
     5401 ggatttaatt ataacggtga gggtgctacg agctcaggcc ttagctcccc ttctgccagt
     5461 gatgtggcta atcagtttaa tagttttggt gggacgcaaa tccgggcatc agatgtctcg
     5521 aatattcgca gtgatggtaa tggtgggtat atggcggata ttcgggggca gattcacact
     5581 gtgtcctctg aggaaggctc aacaacttca acaactgtcg gtggcttcga tggtgtccgc
     5641 agttcttcta ccggtaatgg cggaagtcag ggtgggcagg ggccacaggg acagtctctc
     5701 gttatgcata acgggcagat gggatactgg gaaaaccgat catccggggc tggtaataac
     5761 gaacatacta cccgcgcttt tgtggcagta ggcccatcag aagcagaaaa agctgcacga
     5821 gcggagaagg tagctaagga aaaacagcaa gcagaagagg ctgcaaaagc attcgcagcg
     5881 aaaacagcag cggcatccgc cgctgctgaa aaagaacgtc agaatgcgat ttctgctgcg
     5941 gctgctgcag gtcaacatca aactgttcct gatgcgcgta ataatctgaa tcaggcgact
     6001 gctgaagcat cacgcctgaa aacagtggca gataatgccc ttaatacagc gaagaataag
     6061 cgaaaggaag ctatcgatgc cgtaccagta gcaactcagg ctgagaaaaa atatcaggat
     6121 ctgcagcaaa gcattaaagg cctcactctg aataataacg gccaatacgg tacccaaaaa
     6181 tgggaggtta tcagttcgaa taaagagcat gaccactggg gataccggtt ctatccatct
     6241 gggatcacaa aagctcaggt tgatgcagcg cagaatgatg cggtgaacaa acgaaatgct
     6301 gccaccagtc ttgccagtca ggcaacggcc gctgagcagg ccagtctgca ggcatcagct
     6361 gcctataatg ctgcagaaac gcgccgtcag gctgcccagg ctgcattagc gtccgcagaa
     6421 caagccgccg ctgctgaacg taaacgccag gaggcggaag cagcagccgc cgcggcggca
     6481 gaaaaaaaac gacaggctga tgcagcagcg aaagccgctg aggaggccag agccattgcc
     6541 gaaaaagcaa aagcgctgca ggcccgtcgt acagccgcag ataaactgaa atcttcagag
     6601 attcaggctg tcaggggtat cccggctaca gctgcacctt ttgctattcc tctgacctgg
     6661 tcaactgcgt cacgtggagg ttttactctg gcggctgatg ctgcggccag cttgggagct
     6721 ttcatcagcg aggctctggc gacgttacgt gtcgctgtgg tggctaaccc ggtagcatta
     6781 accattgccg gtcttgtgtt atcgaaatca gttggcgtcg gtagtgatat ggtgcccggt
     6841 cgggatatca gttcgatgat gcctggcgat gcgtttggtc tgcctgatac cgcggcattg
     6901 aataaagcag cagatcagaa aacatctgtc agtatgccgg ttcgtggccg tctggttatg
     6961 aatgatagcg gcattctgga tgttcagctg gtcaaaacca atactgccgg tgccgtgaag
     7021 gtcgctcgtg ctgtactgga taaggaaacc ggctactggg gctatactct tcccgctgtc
     7081 gcagatgttc cggcgcaaac tatttttgtc agcccggctg acgcacctgg tgcaaacgga
     7141 ccactgacgc taagcgggcc ggtaccacta ccggagcgta ttctgcatac cggtgatcag
     7201 atttcggcgc cacaggctac agataaaacc gtgacgccgg tagcggatga cctcgatttt
     7261 gacgatatta ttctggtgtt tccaccggag tccggcctaa aaccgctgta tgtgatgtac
     7321 cgtagccctc gtaacatgcc cggtacagtg agcggcaaag ggcagaacgt tggcaataac
     7381 tggatggggg gggccagtac cggggatggt gctcctgttc cttcccagat tgcggataaa
     7441 ttacgcggga agactttcgg tagttttgat tctttccgtc gggcgttctg gaaagcggtt
     7501 gctgacgatt ctgcactgag caagcaattt agtgaggctg atattaatca gatgaaagct
     7561 ggaagggcac ctactgcgga ct
//
