LOCUS       NZ_CP137432             8334 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP82_L15_04.23_ST147_NDM5 plasmid
            unnamed2, complete sequence.
ACCESSION   NZ_CP137432
VERSION     NZ_CP137432.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951967
            Assembly: GCF_033244325.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 13:10:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,823
            CDSs (total)                      :: 5,699
            Genes (coding)                    :: 5,513
            CDSs (with protein)               :: 5,513
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 98 of 186
            Pseudo Genes (incomplete)         :: 92 of 186
            Pseudo Genes (internal stop)      :: 27 of 186
            Pseudo Genes (multiple problems)  :: 26 of 186
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137432.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP82_L15_04.23_ST147_NDM5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-01"
                     /genotype="ST147"
     gene            99..1214
                     /locus_tag="R5O55_RS00195"
                     /old_locus_tag="R5O55_00195"
     CDS             99..1214
                     /locus_tag="R5O55_RS00195"
                     /old_locus_tag="R5O55_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            1214..1846
                     /locus_tag="R5O55_RS00200"
                     /old_locus_tag="R5O55_00200"
     CDS             1214..1846
                     /locus_tag="R5O55_RS00200"
                     /old_locus_tag="R5O55_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            2212..2454
                     /locus_tag="R5O55_RS00205"
                     /old_locus_tag="R5O55_00205"
     CDS             2212..2454
                     /locus_tag="R5O55_RS00205"
                     /old_locus_tag="R5O55_00205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2800..3201)
                     /locus_tag="R5O55_RS00210"
                     /old_locus_tag="R5O55_00210"
     CDS             complement(2800..3201)
                     /locus_tag="R5O55_RS00210"
                     /old_locus_tag="R5O55_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3201..3392)
                     /locus_tag="R5O55_RS00215"
                     /old_locus_tag="R5O55_00215"
     CDS             complement(3201..3392)
                     /locus_tag="R5O55_RS00215"
                     /old_locus_tag="R5O55_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3808..3921
                     /locus_tag="R5O55_RS00220"
                     /old_locus_tag="R5O55_00220"
                     /pseudo=""
     CDS             3808..3921
                     /locus_tag="R5O55_RS00220"
                     /old_locus_tag="R5O55_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4266..5381
                     /locus_tag="R5O55_RS00225"
                     /old_locus_tag="R5O55_00225"
     CDS             4266..5381
                     /locus_tag="R5O55_RS00225"
                     /old_locus_tag="R5O55_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            5381..6013
                     /locus_tag="R5O55_RS00230"
                     /old_locus_tag="R5O55_00230"
     CDS             5381..6013
                     /locus_tag="R5O55_RS00230"
                     /old_locus_tag="R5O55_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            6379..6621
                     /locus_tag="R5O55_RS00235"
                     /old_locus_tag="R5O55_00235"
     CDS             6379..6621
                     /locus_tag="R5O55_RS00235"
                     /old_locus_tag="R5O55_00235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(6967..7368)
                     /locus_tag="R5O55_RS00240"
                     /old_locus_tag="R5O55_00240"
     CDS             complement(6967..7368)
                     /locus_tag="R5O55_RS00240"
                     /old_locus_tag="R5O55_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7368..7559)
                     /locus_tag="R5O55_RS00245"
                     /old_locus_tag="R5O55_00245"
     CDS             complement(7368..7559)
                     /locus_tag="R5O55_RS00245"
                     /old_locus_tag="R5O55_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7975..8088
                     /locus_tag="R5O55_RS00250"
                     /old_locus_tag="R5O55_00250"
                     /pseudo=""
     CDS             7975..8088
                     /locus_tag="R5O55_RS00250"
                     /old_locus_tag="R5O55_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          3514..3921
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          7681..8088
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 ttgacataaa gtctagctaa tcacaatgaa agctgtacat tttattaagg tactaacaaa
       61 tcgtatggca tataacaaaa acacgaacag aggctatcat gataaaggaa ataagtttaa
      121 agaacttcaa atgcttcaat gagctatatc taaagcaatt aaagacttta aatatcatag
      181 ctgggaaaaa caactacggg aaaacatcta tactggatgc tattttttgt ttttatgatg
      241 taaaaaaccc ggctgttttg ttaaacattc aagcattcag aaaagaaatg gcagaaataa
      301 acaaaaacaa gccattttgg gttagttact ttcatgatat ggacacatca caaaagatgt
      361 ccatagtcat tcgagatgaa cgctctgaag taacacaaac atatgaaaca gaaacaaatc
      421 agagattaga atcaagtttg tctctaaacg ttgacaccgt actatctaac caaaacatac
      481 ctcgtcaaac tatcggttct caggccacag taaactcttt aaaaataaat gtgaccgaga
      541 cacccaatgc gaaaaacagg gtttccaata agcttttctc tatgcaattg aaagaagatg
      601 gcgtagaaat aaaatccgaa ataaaaaacg aaagggatgg gattaattat aaatttaaga
      661 cagcaacaat aataacaaca tccagaaaga taaacaaaga ggcaacaatt acaaatgtat
      721 ctagcctact aacacaaaaa aggaaaaaag acatactaga aaacttaaaa aaaatagatg
      781 acagaattgt agatatagca atatctgcga taggtaacaa taaggaaata tatttagaca
      841 ttggatttag tgagctaaat gaaatatcaa tgcttggcga gggtatcagt cgagcattat
      901 cttttatcag ctcagtgctg gtacaggaaa actcaattat tcttattgat gaaatcgaga
      961 acggaataca ttactcagta attaaagaca taataaaatc attaatttca tctgcaaaac
     1021 aaaacaacaa tcaaatattt gcaacaactc atagtcaaga tgtgatacga gcaataaacg
     1081 aaatagatag caaaaatgaa gatatcgctt atattagact aggaagagaa aagaactccc
     1141 taaaaccaac agccgtacaa ttcaatatgg atgatttctc ctactcagtt gagaacgact
     1201 gggaggtaag ataatgaaag acataaataa tagcaaaaag atcattttcg ttgagggtga
     1261 tgatgatttt cattttctat gcaacctact tgaaatacaa ggcattaccg atgtttttgt
     1321 cgaaaaaata aatggaaaat caaaattaaa acaagcactt agagcattca aaaacattga
     1381 ttctttcgat gaaaaagaaa gtatttttat tatattagat gctgacacat cattttctga
     1441 cacagaaaga tcaataattg ccacattgcg agaactacaa atctcctccc ccagctcgca
     1501 taacgagata gctatgaatg gcaatacaaa aatttctttc tttataatgc caggaaaaga
     1561 caagcaaggt gccattgaag atctgataat tgaacacgca agcactcgag cgttatatga
     1621 atacataata gaatttttcg aaaaaattaa aaataataaa gatgacatct cagcgagtga
     1681 caaagagtac acttatccaa caaacgaaaa aaaagctaag gtacaagtct atcttagctg
     1741 caatagagaa tccgactcaa gaataggaat atccatgaaa aatggaataa taaacacagc
     1801 agacaattgc tttgatgaaa taaaagattt tatttcaaaa atataaatcc aaatcaagcc
     1861 agcccttcac gggctggctt atcaccaggg tgttattaaa cgccggataa cgtatagaaa
     1921 gcatcataca ctttagttat cagcaatctc aaacgtccta aagacatcac cagatgccaa
     1981 aacaatgatt gtgcagatct aatgtaaaag acccagcaca agatgctccc ttaagttata
     2041 ttctctctat tcacttaaaa caatttaaca taaaatcata attgtacgca ccgctaaaac
     2101 ggactcagga cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc
     2161 gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcgca agtgcatagc
     2221 gcggaaattc acaccattca gccaggggaa gtcctgtttc cggcgttctt ctcctccgaa
     2281 aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa
     2341 aaccggtttt cagcaccttc aggaatcggg ttcgtcgacg gccggcaatc acgttatggc
     2401 aagtcgccgg acgactcacc acacgattca ccggcgattc gaatcgtgaa ttgaatcaag
     2461 agtcaccatg agcaaaaaac acccaaagtg atgaattgaa tcaagagtcg attcatcata
     2521 acaatatgat aataaagcgt gattcacata tgaatcgtgg gcaattcgtc agcgattcgc
     2581 catagagtcg tccacgactt ggatctgcac tttgtaacac atcaccgcac atttattttt
     2641 cattaaccca ttgttgtaaa taaggaatct gcaaaatttt aattttgcgt ggggtgtggg
     2701 tatttttagt ggtgaggagc gttcagagat ctgacgattt ttgggaaggt tcaggaactc
     2761 cctgggatgg ccggagagga aaactgaagc atgacggggt caggattttt tccagaattt
     2821 ccaccatggc gccggggatt cagttaattt tgcctggtga ttttcaagaa ggagcatggc
     2881 ctgtttcagg ctgtcaatgt gcgaatcctt ggacaaaagt aaggtgttca ggtgttcaat
     2941 ctcctgtttc agatgacgga tcacctgttc actctggtca gcagaggctg tatcacgctg
     3001 tccagagacc tgtccagaat ggtgttcacc cttcggttgc tcaaaggggc caaagacacg
     3061 taaaagctct gaagtatcta ccatttttgc attgcgctca ccgctacagg tggacaggtc
     3121 acctgcttta attaacctgt gcagtgttgt tctactctta cccgtcagtc gggcagcctc
     3181 actaatagac actctggcca tcagttacca tcctgtaatg catccagtct cgaggacagt
     3241 gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcggc ctcgacatcg
     3301 agatcaagct cgttaagctt ctccagcagg agcagagact gattctggat gaatttagcc
     3361 atgttcagcg ctgttttctg ttgtttcgtc ataagaggcc tcagagcgtc agtggtcagt
     3421 gtagggatct cctcttttcc gcttcctcgc tcactcagtc gctacgctcc tgccgttcgg
     3481 ctgcggcgag cggcaggagc atgcgtagaa acgctcgaga aaaccgagta atcgcatgga
     3541 ggaaagaagg aaaggccgca cagcggccgt ttttccatag gctccgcccc cctgacgagc
     3601 atcacggaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
     3661 aggcgtttcc ccctgatggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     3721 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     3781 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc
     3841 gcctaatccg gtaactatcg tcttgagtcc aacccggaaa gacacgacaa agcgccactg
     3901 gcagcagcca ctggtaactg atttagggag ttagtcttga agttatacgc cagttaaagc
     3961 taaactaaac gaacagtttt ggtgactgcg ctccctttaa gccagttacc tcggttcata
     4021 gagcgccaac tctactgaac ctttcgaaaa aaccgcccgc caaggcggtt tttttcgttc
     4081 acagccggaa gcttacggct gctgccagat aatctcaaga agaaagtaaa aaaaatcgat
     4141 taaaacacaa gcaatcaaag ttaatctttg acataaagtc tagctaatca caatgaaagc
     4201 tgtacatttt attaaggtac taacaaatcg tatggcatat aacaaaaaca cgaacagagg
     4261 ctatcatgat aaaggaaata agtttaaaga acttcaaatg cttcaatgag ctatatctaa
     4321 agcaattaaa gactttaaat atcatagctg ggaaaaacaa ctacgggaaa acatctatac
     4381 tggatgctat tttttgtttt tatgatgtaa aaaacccggc tgttttgtta aacattcaag
     4441 cattcagaaa agaaatggca gaaataaaca aaaacaagcc attttgggtt agttactttc
     4501 atgatatgga cacatcacaa aagatgtcca tagtcattcg agatgaacgc tctgaagtaa
     4561 cacaaacata tgaaacagaa acaaatcaga gattagaatc aagtttgtct ctaaacgttg
     4621 acaccgtact atctaaccaa aacatacctc gtcaaactat cggttctcag gccacagtaa
     4681 actctttaaa aataaatgtg accgagacac ccaatgcgaa aaacagggtt tccaataagc
     4741 ttttctctat gcaattgaaa gaagatggcg tagaaataaa atccgaaata aaaaacgaaa
     4801 gggatgggat taattataaa tttaagacag caacaataat aacaacatcc agaaagataa
     4861 acaaagaggc aacaattaca aatgtatcta gcctactaac acaaaaaagg aaaaaagaca
     4921 tactagaaaa cttaaaaaaa atagatgaca gaattgtaga tatagcaata tctgcgatag
     4981 gtaacaataa ggaaatatat ttagacattg gatttagtga gctaaatgaa atatcaatgc
     5041 ttggcgaggg tatcagtcga gcattatctt ttatcagctc agtgctggta caggaaaact
     5101 caattattct tattgatgaa atcgagaacg gaatacatta ctcagtaatt aaagacataa
     5161 taaaatcatt aatttcatct gcaaaacaaa acaacaatca aatatttgca acaactcata
     5221 gtcaagatgt gatacgagca ataaacgaaa tagatagcaa aaatgaagat atcgcttata
     5281 ttagactagg aagagaaaag aactccctaa aaccaacagc cgtacaattc aatatggatg
     5341 atttctccta ctcagttgag aacgactggg aggtaagata atgaaagaca taaataatag
     5401 caaaaagatc attttcgttg agggtgatga tgattttcat tttctatgca acctacttga
     5461 aatacaaggc attaccgatg tttttgtcga aaaaataaat ggaaaatcaa aattaaaaca
     5521 agcacttaga gcattcaaaa acattgattc tttcgatgaa aaagaaagta tttttattat
     5581 attagatgct gacacatcat tttctgacac agaaagatca ataattgcca cattgcgaga
     5641 actacaaatc tcctccccca gctcgcataa cgagatagct atgaatggca atacaaaaat
     5701 ttctttcttt ataatgccag gaaaagacaa gcaaggtgcc attgaagatc tgataattga
     5761 acacgcaagc actcgagcgt tatatgaata cataatagaa tttttcgaaa aaattaaaaa
     5821 taataaagat gacatctcag cgagtgacaa agagtacact tatccaacaa acgaaaaaaa
     5881 agctaaggta caagtctatc ttagctgcaa tagagaatcc gactcaagaa taggaatatc
     5941 catgaaaaat ggaataataa acacagcaga caattgcttt gatgaaataa aagattttat
     6001 ttcaaaaata taaatccaaa tcaagccagc ccttcacggg ctggcttatc accagggtgt
     6061 tattaaacgc cggataacgt atagaaagca tcatacactt tagttatcag caatctcaaa
     6121 cgtcctaaag acatcaccag atgccaaaac aatgattgtg cagatctaat gtaaaagacc
     6181 cagcacaaga tgctccctta agttatattc tctctattca cttaaaacaa tttaacataa
     6241 aatcataatt gtacgcaccg ctaaaacgga ctcaggacgt ttctgacggg aaaccggtaa
     6301 aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat
     6361 gcatgagtat gcgcgcaagt gcatagcgcg gaaattcaca ccattcagcc aggggaagtc
     6421 ctgtttccgg cgttcttctc ctccgaaaac ggtgaacggg gacgcaattt attgcgtggc
     6481 cgtgaagcgt tgccggcgca gccggaaaac cggttttcag caccttcagg aatcgggttc
     6541 gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg
     6601 gcgattcgaa tcgtgaattg aatcaagagt caccatgagc aaaaaacacc caaagtgatg
     6661 aattgaatca agagtcgatt catcataaca atatgataat aaagcgtgat tcacatatga
     6721 atcgtgggca attcgtcagc gattcgccat agagtcgtcc acgacttgga tctgcacttt
     6781 gtaacacatc accgcacatt tatttttcat taacccattg ttgtaaataa ggaatctgca
     6841 aaattttaat tttgcgtggg gtgtgggtat ttttagtggt gaggagcgtt cagagatctg
     6901 acgatttttg ggaaggttca ggaactccct gggatggccg gagaggaaaa ctgaagcatg
     6961 acggggtcag gattttttcc agaatttcca ccatggcgcc ggggattcag ttaattttgc
     7021 ctggtgattt tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg aatccttgga
     7081 caaaagtaag gtgttcaggt gttcaatctc ctgtttcaga tgacggatca cctgttcact
     7141 ctggtcagca gaggctgtat cacgctgtcc agagacctgt ccagaatggt gttcaccctt
     7201 cggttgctca aaggggccaa agacacgtaa aagctctgaa gtatctacca tttttgcatt
     7261 gcgctcaccg ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gtgttgttct
     7321 actcttaccc gtcagtcggg cagcctcact aatagacact ctggccatca gttaccatcc
     7381 tgtaatgcat ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt
     7441 ttctcacaaa ggtcggcctc gacatcgaga tcaagctcgt taagcttctc cagcaggagc
     7501 agagactgat tctggatgaa tttagccatg ttcagcgctg ttttctgttg tttcgtcata
     7561 agaggcctca gagcgtcagt ggtcagtgta gggatctcct cttttccgct tcctcgctca
     7621 ctcagtcgct acgctcctgc cgttcggctg cggcgagcgg caggagcatg cgtagaaacg
     7681 ctcgagaaaa ccgagtaatc gcatggagga aagaaggaaa ggccgcacag cggccgtttt
     7741 tccataggct ccgcccccct gacgagcatc acggaatctg acgctcaaat cagtggtggc
     7801 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcgtgcgct
     7861 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     7921 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     7981 gaaccccccg ttcagtccga ctgctgcgcc taatccggta actatcgtct tgagtccaac
     8041 ccggaaagac acgacaaagc gccactggca gcagccactg gtaactgatt tagggagtta
     8101 gtcttgaagt tatacgccag ttaaagctaa actaaacgaa cagttttggt gactgcgctc
     8161 cctttaagcc agttacctcg gttcatagag cgccaactct actgaacctt tcgaaaaaac
     8221 cgcccgccaa ggcggttttt ttcgttcaca gccggaagct tacggctgct gccagataat
     8281 ctcaagaaga aagtaaaaaa aatcgattaa aacacaagca atcaaagtta atct
//
