LOCUS       NZ_CP137430             4167 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP88_L3_06.23_ST147_NDM14 plasmid
            unnamed3, complete sequence.
ACCESSION   NZ_CP137430
VERSION     NZ_CP137430.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951969
            Assembly: GCF_033242065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 63x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 10:55:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,848
            CDSs (total)                      :: 5,725
            Genes (coding)                    :: 5,525
            CDSs (with protein)               :: 5,525
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 110 of 200
            Pseudo Genes (incomplete)         :: 97 of 200
            Pseudo Genes (internal stop)      :: 27 of 200
            Pseudo Genes (multiple problems)  :: 29 of 200
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137430.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP88_L3_06.23_ST147_NDM14"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-01"
                     /genotype="ST147"
     gene            complement(join(4041..4167,1..989))
                     /locus_tag="R5O57_RS29220"
                     /old_locus_tag="R5O57_29220"
     CDS             complement(join(4041..4167,1..989))
                     /locus_tag="R5O57_RS29220"
                     /old_locus_tag="R5O57_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(1334..1447)
                     /locus_tag="R5O57_RS29225"
                     /old_locus_tag="R5O57_29225"
                     /pseudo=""
     CDS             complement(1334..1447)
                     /locus_tag="R5O57_RS29225"
                     /old_locus_tag="R5O57_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1863..2054
                     /locus_tag="R5O57_RS29230"
                     /old_locus_tag="R5O57_29230"
     CDS             1863..2054
                     /locus_tag="R5O57_RS29230"
                     /old_locus_tag="R5O57_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2054..2455
                     /locus_tag="R5O57_RS29235"
                     /old_locus_tag="R5O57_29235"
     CDS             2054..2455
                     /locus_tag="R5O57_RS29235"
                     /old_locus_tag="R5O57_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2801..3043)
                     /locus_tag="R5O57_RS29240"
                     /old_locus_tag="R5O57_29240"
     CDS             complement(2801..3043)
                     /locus_tag="R5O57_RS29240"
                     /old_locus_tag="R5O57_29240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3409..4041)
                     /locus_tag="R5O57_RS29245"
                     /old_locus_tag="R5O57_29245"
     CDS             complement(3409..4041)
                     /locus_tag="R5O57_RS29245"
                     /old_locus_tag="R5O57_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     Typing          complement(1335..1742)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tctatttcgt ttattgctcg tatcacatct tgactatgag ttgttgcaaa tatttgattg
       61 ttgttttgtt ttgcagatga aattaatgat tttattatgt ctttaattac tgagtaatgt
      121 attccgttct cgatttcatc aataagaata attgagtttt cctgtaccag cactgagctg
      181 ataaaagata atgctcgact gataccctcg ccaagcattg atatttcatt tagctcacta
      241 aatccaatgt ctaaatatat ttccttattg ttacctatcg cagatattgc tatatctaca
      301 attctgtcat ctattttttt taagttttct agtatgtctt ttttcctttt ttgtgttagt
      361 aggctagata catttgtaat tgttgcctct ttgtttatct ttctggatgt tgttattatt
      421 gttgctgtct taaatttata attaatccca tccctttcgt tttttatttc ggattttatt
      481 tctacgccat cttctttcaa ttgcatagag aaaagcttat tggaaaccct gtttttcgca
      541 ttgggtgtct cggtcacatt tatttttaaa gagtttactg tggcctgaga accgatagtt
      601 tgacgaggta tgttttggtt agatagtacg gtgtcaacgt ttagagacaa acttgattct
      661 aatctctgat ttgtttctgt ttcatatgtt tgtgttactt cagagcgttc atctcgaatg
      721 actatggaca tcttttgtga tgtgtccata tcatgaaagt aactaaccca aaatggcttg
      781 tttttgttta tttctgccat ttcttttctg aatgcttgaa tgtttaacaa aacagccggg
      841 ttttttacat cataaaaaca aaaaatagca tccagtatag atgttttccc gtagttgttt
      901 ttcccagcta tgatatttaa agtctttaat tgctttagat atagctcatt gaagcatttg
      961 aagttcttta aacttatttc ctttatcatg atagcctctg ttcgtgtttt tgttatatgc
     1021 catacgattt gttagtacct taataaaatg tacagctttc attgtgatta gctagacttt
     1081 atgtcaaaga ttaactttga ttgcttgtgt tttaatcgat tttttttact ttcttcttga
     1141 gattatctgg cagcagccgt aagcttccgg ctgtgaacga aaaaaaccgc cttggcgggc
     1201 ggttttttcg aaaggttcag tagagttggc gctctatgaa ccgaggtaac tggcttaaag
     1261 ggagcgcagt caccaaaact gttcgtttag tttagcttta actggcgtat aacttcaaga
     1321 ctaactccct aaatcagtta ccagtggctg ctgccagtgg cgctttgtcg tgtctttccg
     1381 ggttggactc aagacgatag ttaccggatt aggcgcagca gtcggactga acggggggtt
     1441 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
     1501 tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
     1561 gagcgcacga gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1621 cgccaccact gatttgagcg tcagattccg tgatgctcgt caggggggcg gagcctatgg
     1681 aaaaacggcc gctgtgcggc ctttccttct ttcctccatg cgattactcg gttttctcga
     1741 gcgtttctac gcatgctcct gccgctcgcc gcagccgaac ggcaggagcg tagcgactga
     1801 gtgagcgagg aagcggaaaa gaggagatcc ctacactgac cactgacgct ctgaggcctc
     1861 ttatgacgaa acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc
     1921 tgctcctgct ggagaagctt aacgagcttg atctcgatgt cgaggccgac ctttgtgaga
     1981 aactccacga tgatgcggag catcttttcc gtacactgtc ctcgagactg gatgcattac
     2041 aggatggtaa ctgatggcca gagtgtctat tagtgaggct gcccgactga cgggtaagag
     2101 tagaacaaca ctgcacaggt taattaaagc aggtgacctg tccacctgta gcggtgagcg
     2161 caatgcaaaa atggtagata cttcagagct tttacgtgtc tttggcccct ttgagcaacc
     2221 gaagggtgaa caccattctg gacaggtctc tggacagcgt gatacagcct ctgctgacca
     2281 gagtgaacag gtgatccgtc atctgaaaca ggagattgaa cacctgaaca ccttactttt
     2341 gtccaaggat tcgcacattg acagcctgaa acaggccatg ctccttcttg aaaatcacca
     2401 ggcaaaatta actgaatccc cggcgccatg gtggaaattc tggaaaaaat cctgaccccg
     2461 tcatgcttca gttttcctct ccggccatcc cagggagttc ctgaaccttc ccaaaaatcg
     2521 tcagatctct gaacgctcct caccactaaa aatacccaca ccccacgcaa aattaaaatt
     2581 ttgcagattc cttatttaca acaatgggtt aatgaaaaat aaatgtgcgg tgatgtgtta
     2641 caaagtgcag atccaagtcg tggacgactc tatggcgaat cgctgacgaa ttgcccacga
     2701 ttcatatgtg aatcacgctt tattatcata ttgttatgat gaatcgactc ttgattcaat
     2761 tcatcacttt gggtgttttt tgctcatggt gactcttgat tcaattcacg attcgaatcg
     2821 ccggtgaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc cggccgtcga
     2881 cgaacccgat tcctgaaggt gctgaaaacc ggttttccgg ctgcgccggc aacgcttcac
     2941 ggccacgcaa taaattgcgt ccccgttcac cgttttcgga ggagaagaac gccggaaaca
     3001 ggacttcccc tggctgaatg gtgtgaattt ccgcgctatg cacttgcgcg catactcatg
     3061 catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt
     3121 tttaccggtt tcccgtcaga aacgtcctga gtccgtttta gcggtgcgta caattatgat
     3181 tttatgttaa attgttttaa gtgaatagag agaatataac ttaagggagc atcttgtgct
     3241 gggtctttta cattagatct gcacaatcat tgttttggca tctggtgatg tctttaggac
     3301 gtttgagatt gctgataact aaagtgtatg atgctttcta tacgttatcc ggcgtttaat
     3361 aacaccctgg tgataagcca gcccgtgaag ggctggcttg atttggattt atatttttga
     3421 aataaaatct tttatttcat caaagcaatt gtctgctgtg tttattattc catttttcat
     3481 ggatattcct attcttgagt cggattctct attgcagcta agatagactt gtaccttagc
     3541 ttttttttcg tttgttggat aagtgtactc tttgtcactc gctgagatgt catctttatt
     3601 atttttaatt ttttcgaaaa attctattat gtattcatat aacgctcgag tgcttgcgtg
     3661 ttcaattatc agatcttcaa tggcaccttg cttgtctttt cctggcatta taaagaaaga
     3721 aatttttgta ttgccattca tagctatctc gttatgcgag ctgggggagg agatttgtag
     3781 ttctcgcaat gtggcaatta ttgatctttc tgtgtcagaa aatgatgtgt cagcatctaa
     3841 tataataaaa atactttctt tttcatcgaa agaatcaatg tttttgaatg ctctaagtgc
     3901 ttgttttaat tttgattttc catttatttt ttcgacaaaa acatcggtaa tgccttgtat
     3961 ttcaagtagg ttgcatagaa aatgaaaatc atcatcaccc tcaacgaaaa tgatcttttt
     4021 gctattattt atgtctttca ttatcttacc tcccagtcgt tctcaactga gtaggagaaa
     4081 tcatccatat tgaattgtac ggctgttggt tttagggagt tcttttctct tcctagtcta
     4141 atataagcga tatcttcatt tttgcta
//
