LOCUS       NZ_CP137421            12500 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP10_L3_11.22_ST147 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP137421
VERSION     NZ_CP137421.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951957
            Assembly: GCF_033257165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12500)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 74x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:42:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,660
            CDSs (total)                      :: 5,536
            Genes (coding)                    :: 5,342
            CDSs (with protein)               :: 5,342
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 7, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 7, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 90 of 194
            Pseudo Genes (incomplete)         :: 113 of 194
            Pseudo Genes (internal stop)      :: 27 of 194
            Pseudo Genes (multiple problems)  :: 28 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137421.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP10_L3_11.22_ST147"
                     /isolation_source="neonatal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-03"
                     /genotype="ST147"
     gene            78..191
                     /locus_tag="R5O45_RS00800"
                     /old_locus_tag="R5O45_00800"
                     /pseudo=""
     CDS             78..191
                     /locus_tag="R5O45_RS00800"
                     /old_locus_tag="R5O45_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            536..1651
                     /locus_tag="R5O45_RS00805"
                     /old_locus_tag="R5O45_00805"
     CDS             536..1651
                     /locus_tag="R5O45_RS00805"
                     /old_locus_tag="R5O45_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            1651..2283
                     /locus_tag="R5O45_RS00810"
                     /old_locus_tag="R5O45_00810"
     CDS             1651..2283
                     /locus_tag="R5O45_RS00810"
                     /old_locus_tag="R5O45_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            2649..2891
                     /locus_tag="R5O45_RS00815"
                     /old_locus_tag="R5O45_00815"
     CDS             2649..2891
                     /locus_tag="R5O45_RS00815"
                     /old_locus_tag="R5O45_00815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3237..3638)
                     /locus_tag="R5O45_RS00820"
                     /old_locus_tag="R5O45_00820"
     CDS             complement(3237..3638)
                     /locus_tag="R5O45_RS00820"
                     /old_locus_tag="R5O45_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3638..3829)
                     /locus_tag="R5O45_RS00825"
                     /old_locus_tag="R5O45_00825"
     CDS             complement(3638..3829)
                     /locus_tag="R5O45_RS00825"
                     /old_locus_tag="R5O45_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4245..4358
                     /locus_tag="R5O45_RS00830"
                     /old_locus_tag="R5O45_00830"
                     /pseudo=""
     CDS             4245..4358
                     /locus_tag="R5O45_RS00830"
                     /old_locus_tag="R5O45_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4703..5818
                     /locus_tag="R5O45_RS00835"
                     /old_locus_tag="R5O45_00835"
     CDS             4703..5818
                     /locus_tag="R5O45_RS00835"
                     /old_locus_tag="R5O45_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            5818..6450
                     /locus_tag="R5O45_RS00840"
                     /old_locus_tag="R5O45_00840"
     CDS             5818..6450
                     /locus_tag="R5O45_RS00840"
                     /old_locus_tag="R5O45_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            6816..7058
                     /locus_tag="R5O45_RS00845"
                     /old_locus_tag="R5O45_00845"
     CDS             6816..7058
                     /locus_tag="R5O45_RS00845"
                     /old_locus_tag="R5O45_00845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(7404..7805)
                     /locus_tag="R5O45_RS00850"
                     /old_locus_tag="R5O45_00850"
     CDS             complement(7404..7805)
                     /locus_tag="R5O45_RS00850"
                     /old_locus_tag="R5O45_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7805..7996)
                     /locus_tag="R5O45_RS00855"
                     /old_locus_tag="R5O45_00855"
     CDS             complement(7805..7996)
                     /locus_tag="R5O45_RS00855"
                     /old_locus_tag="R5O45_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8412..8525
                     /locus_tag="R5O45_RS00860"
                     /old_locus_tag="R5O45_00860"
                     /pseudo=""
     CDS             8412..8525
                     /locus_tag="R5O45_RS00860"
                     /old_locus_tag="R5O45_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            8870..9985
                     /locus_tag="R5O45_RS00865"
                     /old_locus_tag="R5O45_00865"
     CDS             8870..9985
                     /locus_tag="R5O45_RS00865"
                     /old_locus_tag="R5O45_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            9985..10536
                     /locus_tag="R5O45_RS00870"
                     /old_locus_tag="R5O45_00870"
     CDS             9985..10536
                     /locus_tag="R5O45_RS00870"
                     /old_locus_tag="R5O45_00870"
                     /inference="COORDINATES: protein motif:HMM:NF022971.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_317978274.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERLIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKLRYKSILAAIENPTQE"
                     /name="DUF3226 domain-containing protein"
     gene            10982..11224
                     /locus_tag="R5O45_RS00875"
                     /old_locus_tag="R5O45_00875"
     CDS             10982..11224
                     /locus_tag="R5O45_RS00875"
                     /old_locus_tag="R5O45_00875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(11570..11971)
                     /locus_tag="R5O45_RS00880"
                     /old_locus_tag="R5O45_00880"
     CDS             complement(11570..11971)
                     /locus_tag="R5O45_RS00880"
                     /old_locus_tag="R5O45_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(11971..12162)
                     /locus_tag="R5O45_RS00885"
                     /old_locus_tag="R5O45_00885"
     CDS             complement(11971..12162)
                     /locus_tag="R5O45_RS00885"
                     /old_locus_tag="R5O45_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..191
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.764"
                     /coverage="100.0"
                     /evalue="7.84e-78"
     Typing          3951..4358
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          8118..8525
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          12284..12500
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.743"
                     /coverage="99.0"
                     /evalue="8.700000000000001e-80"
ORIGIN
        1 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
       61 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gcctaatccg
      121 gtaactatcg tcttgagtcc aacccggaaa gacacgacaa agcgccactg gcagcagcca
      181 ctggtaactg atttagggag ttagtcttga agttatacgc cagttaaagc taaactaaac
      241 gaacagtttt ggtgactgcg ctccctttaa gccagttacc tcggttcata gagcgccaac
      301 tctactgaac ctttcgaaaa aaccgcccgc caaggcggtt tttttcgttc acagccggaa
      361 gcttacggct gctgccagat aatctcaaga agaaagtaaa aaaaatcgat taaaacacaa
      421 gcaatcaaag ttaatctttg acataaagtc tagctaatca caatgaaagc tgtacatttt
      481 attaaggtac taacaaatcg tatggcatat aacaaaaaca cgaacagagg ctatcatgat
      541 aaaggaaata agtttaaaga acttcaaatg cttcaatgag ctatatctaa agcaattaaa
      601 gactttaaat atcatagctg ggaaaaacaa ctacgggaaa acatctatac tggatgctat
      661 tttttgtttt tatgatgtaa aaaacccggc tgttttgtta aacattcaag cattcagaaa
      721 agaaatggca gaaataaaca aaaacaagcc attttgggtt agttactttc atgatatgga
      781 cacatcacaa aagatgtcca tagtcattcg agatgaacgc tctgaagtaa cacaaacata
      841 tgaaacagaa acaaatcaga gattagaatc aagtttgtct ctaaacgttg acaccgtact
      901 atctaaccaa aacatacctc gtcaaactat cggttctcag gccacagtaa actctttaaa
      961 aataaatgtg accgagacac ccaatgcgaa aaacagggtt tccaataagc ttttctctat
     1021 gcaattgaaa gaagatggcg tagaaataaa atccgaaata aaaaacgaaa gggatgggat
     1081 taattataaa tttaagacag caacaataat aacaacatcc agaaagataa acaaagaggc
     1141 aacaattaca aatgtatcta gcctactaac acaaaaaagg aaaaaagaca tactagaaaa
     1201 cttaaaaaaa atagatgaca gaattgtaga tatagcaata tctgcgatag gtaacaataa
     1261 ggaaatatat ttagacattg gatttagtga gctaaatgaa atatcaatgc ttggcgaggg
     1321 tatcagtcga gcattatctt ttatcagctc agtgctggta caggaaaact caattattct
     1381 tattgatgaa atcgagaacg gaatacatta ctcagtaatt aaagacataa taaaatcatt
     1441 aatttcatct gcaaaacaaa acaacaatca aatatttgca acaactcata gtcaagatgt
     1501 gatacgagca ataaacgaaa tagatagcaa aaatgaagat atcgcttata ttagactagg
     1561 aagagaaaag aactccctaa aaccaacagc cgtacaattc aatatggatg atttctccta
     1621 ctcagttgag aacgactggg aggtaagata atgaaagaca taaataatag caaaaagatc
     1681 attttcgttg agggtgatga tgattttcat tttctatgca acctacttga aatacaaggc
     1741 attaccgatg tttttgtcga aaaaataaat ggaaaatcaa aattaaaaca agcacttaga
     1801 gcattcaaaa acattgattc tttcgatgaa aaagaaagta tttttattat attagatgct
     1861 gacacatcat tttctgacac agaaagatca ataattgcca cattgcgaga actacaaatc
     1921 tcctccccca gctcgcataa cgagatagct atgaatggca atacaaaaat ttctttcttt
     1981 ataatgccag gaaaagacaa gcaaggtgcc attgaagatc tgataattga acacgcaagc
     2041 actcgagcgt tatatgaata cataatagaa tttttcgaaa aaattaaaaa taataaagat
     2101 gacatctcag cgagtgacaa agagtacact tatccaacaa acgaaaaaaa agctaaggta
     2161 caagtctatc ttagctgcaa tagagaatcc gactcaagaa taggaatatc catgaaaaat
     2221 ggaataataa acacagcaga caattgcttt gatgaaataa aagattttat ttcaaaaata
     2281 taaatccaaa tcaagccagc ccttcacggg ctggcttatc accagggtgt tattaaacgc
     2341 cggataacgt atagaaagca tcatacactt tagttatcag caatctcaaa cgtcctaaag
     2401 acatcaccag atgccaaaac aatgattgtg cagatctaat gtaaaagacc cagcacaaga
     2461 tgctccctta agttatattc tctctattca cttaaaacaa tttaacataa aatcataatt
     2521 gtacgcaccg ctaaaacgga ctcaggacgt ttctgacggg aaaccggtaa aacaggcaac
     2581 aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat gcatgagtat
     2641 gcgcgcaagt gcatagcgcg gaaattcaca ccattcagcc aggggaagtc ctgtttccgg
     2701 cgttcttctc ctccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt
     2761 tgccggcgca gccggaaaac cggttttcag caccttcagg aatcgggttc gtcgacggcc
     2821 ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgattcgaa
     2881 tcgtgaattg aatcaagagt caccatgagc aaaaaacacc caaagtgatg aattgaatca
     2941 agagtcgatt catcataaca atatgataat aaagcgtgat tcacatatga atcgtgggca
     3001 attcgtcagc gattcgccat agagtcgtcc acgacttgga tctgcacttt gtaacacatc
     3061 accgcacatt tatttttcat taacccattg ttgtaaataa ggaatctgca aaattttaat
     3121 tttgcgtggg gtgtgggtat ttttagtggt gaggagcgtt cagagatctg acgatttttg
     3181 ggaaggttca ggaactccct gggatggccg gagaggaaaa ctgaagcatg acggggtcag
     3241 gattttttcc agaatttcca ccatggcgcc ggggattcag ttaattttgc ctggtgattt
     3301 tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg aatccttgga caaaagtaag
     3361 gtgttcaggt gttcaatctc ctgtttcaga tgacggatca cctgttcact ctggtcagca
     3421 gaggctgtat cacgctgtcc agagacctgt ccagaatggt gttcaccctt cggttgctca
     3481 aaggggccaa agacacgtaa aagctctgaa gtatctacca tttttgcatt gcgctcaccg
     3541 ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gtgttgttct actcttaccc
     3601 gtcagtcggg cagcctcact aatagacact ctggccatca gttaccatcc tgtaatgcat
     3661 ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt ttctcacaaa
     3721 ggtcggcctc gacatcgaga tcaagctcgt taagcttctc cagcaggagc agagactgat
     3781 tctggatgaa tttagccatg ttcagcgctg ttttctgttg tttcgtcata agaggcctca
     3841 gagcgtcagt ggtcagtgta gggatctcct cttttccgct tcctcgctca ctcagtcgct
     3901 acgctcctgc cgttcggctg cggcgagcgg caggagcatg cgtagaaacg ctcgagaaaa
     3961 ccgagtaatc gcatggagga aagaaggaaa ggccgcacag cggccgtttt tccataggct
     4021 ccgcccccct gacgagcatc acggaatctg acgctcaaat cagtggtggc gaaacccgac
     4081 aggactataa agataccagg cgtttccccc tgatggctcc ctcgtgcgct ctcctgttcc
     4141 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
     4201 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     4261 ttcagtccga ctgctgcgcc taatccggta actatcgtct tgagtccaac ccggaaagac
     4321 acgacaaagc gccactggca gcagccactg gtaactgatt tagggagtta gtcttgaagt
     4381 tatacgccag ttaaagctaa actaaacgaa cagttttggt gactgcgctc cctttaagcc
     4441 agttacctcg gttcatagag cgccaactct actgaacctt tcgaaaaaac cgcccgccaa
     4501 ggcggttttt ttcgttcaca gccggaagct tacggctgct gccagataat ctcaagaaga
     4561 aagtaaaaaa aatcgattaa aacacaagca atcaaagtta atctttgaca taaagtctag
     4621 ctaatcacaa tgaaagctgt acattttatt aaggtactaa caaatcgtat ggcatataac
     4681 aaaaacacga acagaggcta tcatgataaa ggaaataagt ttaaagaact tcaaatgctt
     4741 caatgagcta tatctaaagc aattaaagac tttaaatatc atagctggga aaaacaacta
     4801 cgggaaaaca tctatactgg atgctatttt ttgtttttat gatgtaaaaa acccggctgt
     4861 tttgttaaac attcaagcat tcagaaaaga aatggcagaa ataaacaaaa acaagccatt
     4921 ttgggttagt tactttcatg atatggacac atcacaaaag atgtccatag tcattcgaga
     4981 tgaacgctct gaagtaacac aaacatatga aacagaaaca aatcagagat tagaatcaag
     5041 tttgtctcta aacgttgaca ccgtactatc taaccaaaac atacctcgtc aaactatcgg
     5101 ttctcaggcc acagtaaact ctttaaaaat aaatgtgacc gagacaccca atgcgaaaaa
     5161 cagggtttcc aataagcttt tctctatgca attgaaagaa gatggcgtag aaataaaatc
     5221 cgaaataaaa aacgaaaggg atgggattaa ttataaattt aagacagcaa caataataac
     5281 aacatccaga aagataaaca aagaggcaac aattacaaat gtatctagcc tactaacaca
     5341 aaaaaggaaa aaagacatac tagaaaactt aaaaaaaata gatgacagaa ttgtagatat
     5401 agcaatatct gcgataggta acaataagga aatatattta gacattggat ttagtgagct
     5461 aaatgaaata tcaatgcttg gcgagggtat cagtcgagca ttatctttta tcagctcagt
     5521 gctggtacag gaaaactcaa ttattcttat tgatgaaatc gagaacggaa tacattactc
     5581 agtaattaaa gacataataa aatcattaat ttcatctgca aaacaaaaca acaatcaaat
     5641 atttgcaaca actcatagtc aagatgtgat acgagcaata aacgaaatag atagcaaaaa
     5701 tgaagatatc gcttatatta gactaggaag agaaaagaac tccctaaaac caacagccgt
     5761 acaattcaat atggatgatt tctcctactc agttgagaac gactgggagg taagataatg
     5821 aaagacataa ataatagcaa aaagatcatt ttcgttgagg gtgatgatga ttttcatttt
     5881 ctatgcaacc tacttgaaat acaaggcatt accgatgttt ttgtcgaaaa aataaatgga
     5941 aaatcaaaat taaaacaagc acttagagca ttcaaaaaca ttgattcttt cgatgaaaaa
     6001 gaaagtattt ttattatatt agatgctgac acatcatttt ctgacacaga aagatcaata
     6061 attgccacat tgcgagaact acaaatctcc tcccccagct cgcataacga gatagctatg
     6121 aatggcaata caaaaatttc tttctttata atgccaggaa aagacaagca aggtgccatt
     6181 gaagatctga taattgaaca cgcaagcact cgagcgttat atgaatacat aatagaattt
     6241 ttcgaaaaaa ttaaaaataa taaagatgac atctcagcga gtgacaaaga gtacacttat
     6301 ccaacaaacg aaaaaaaagc taaggtacaa gtctatctta gctgcaatag agaatccgac
     6361 tcaagaatag gaatatccat gaaaaatgga ataataaaca cagcagacaa ttgctttgat
     6421 gaaataaaag attttatttc aaaaatataa atccaaatca agccagccct tcacgggctg
     6481 gcttatcacc agggtgttat taaacgccgg ataacgtata gaaagcatca tacactttag
     6541 ttatcagcaa tctcaaacgt cctaaagaca tcaccagatg ccaaaacaat gattgtgcag
     6601 atctaatgta aaagacccag cacaagatgc tcccttaagt tatattctct ctattcactt
     6661 aaaacaattt aacataaaat cataattgta cgcaccgcta aaacggactc aggacgtttc
     6721 tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg
     6781 ctctgttttt acggcatgca tgagtatgcg cgcaagtgca tagcgcggaa attcacacca
     6841 ttcagccagg ggaagtcctg tttccggcgt tcttctcctc cgaaaacggt gaacggggac
     6901 gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac
     6961 cttcaggaat cgggttcgtc gacggccggc aatcacgtta tggcaagtcg ccggacgact
     7021 caccacacga ttcaccggcg attcgaatcg tgaattgaat caagagtcac catgagcaaa
     7081 aaacacccaa agtgatgaat tgaatcaaga gtcgattcat cataacaata tgataataaa
     7141 gcgtgattca catatgaatc gtgggcaatt cgtcagcgat tcgccataga gtcgtccacg
     7201 acttggatct gcactttgta acacatcacc gcacatttat ttttcattaa cccattgttg
     7261 taaataagga atctgcaaaa ttttaatttt gcgtggggtg tgggtatttt tagtggtgag
     7321 gagcgttcag agatctgacg atttttggga aggttcagga actccctggg atggccggag
     7381 aggaaaactg aagcatgacg gggtcaggat tttttccaga atttccacca tggcgccggg
     7441 gattcagtta attttgcctg gtgattttca agaaggagca tggcctgttt caggctgtca
     7501 atgtgcgaat ccttggacaa aagtaaggtg ttcaggtgtt caatctcctg tttcagatga
     7561 cggatcacct gttcactctg gtcagcagag gctgtatcac gctgtccaga gacctgtcca
     7621 gaatggtgtt cacccttcgg ttgctcaaag gggccaaaga cacgtaaaag ctctgaagta
     7681 tctaccattt ttgcattgcg ctcaccgcta caggtggaca ggtcacctgc tttaattaac
     7741 ctgtgcagtg ttgttctact cttacccgtc agtcgggcag cctcactaat agacactctg
     7801 gccatcagtt accatcctgt aatgcatcca gtctcgagga cagtgtacgg aaaagatgct
     7861 ccgcatcatc gtggagtttc tcacaaaggt cggcctcgac atcgagatca agctcgttaa
     7921 gcttctccag caggagcaga gactgattct ggatgaattt agccatgttc agcgctgttt
     7981 tctgttgttt cgtcataaga ggcctcagag cgtcagtggt cagtgtaggg atctcctctt
     8041 ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt tcggctgcgg cgagcggcag
     8101 gagcatgcgt agaaacgctc gagaaaaccg agtaatcgca tggaggaaag aaggaaaggc
     8161 cgcacagcgg ccgtttttcc ataggctccg cccccctgac gagcatcacg gaatctgacg
     8221 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga
     8281 tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     8341 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     8401 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctaa tccggtaact
     8461 atcgtcttga gtccaacccg gaaagacacg acaaagcgcc actggcagca gccactggta
     8521 actgatttag ggagttagtc ttgaagttat acgccagtta aagctaaact aaacgaacag
     8581 ttttggtgac tgcgctccct ttaagccagt tacctcggtt catagagcgc caactctact
     8641 gaacctttcg aaaaaaccgc ccgccaaggc ggtttttttc gttcacagcc ggaagcttac
     8701 ggctgctgcc agataatctc aagaagaaag taaaaaaaat cgattaaaac acaagcaatc
     8761 aaagttaatc tttgacataa agtctagcta atcacaatga aagctgtaca ttttattaag
     8821 gtactaacaa atcgtatggc atataacaaa aacacgaaca gaggctatca tgataaagga
     8881 aataagttta aagaacttca aatgcttcaa tgagctatat ctaaagcaat taaagacttt
     8941 aaatatcata gctgggaaaa acaactacgg gaaaacatct atactggatg ctattttttg
     9001 tttttatgat gtaaaaaacc cggctgtttt gttaaacatt caagcattca gaaaagaaat
     9061 ggcagaaata aacaaaaaca agccattttg ggttagttac tttcatgata tggacacatc
     9121 acaaaagatg tccatagtca ttcgagatga acgctctgaa gtaacacaaa catatgaaac
     9181 agaaacaaat cagagattag aatcaagttt gtctctaaac gttgacaccg tactatctaa
     9241 ccaaaacata cctcgtcaaa ctatcggttc tcaggccaca gtaaactctt taaaaataaa
     9301 tgtgaccgag acacccaatg cgaaaaacag ggtttccaat aagcttttct ctatgcaatt
     9361 gaaagaagat ggcgtagaaa taaaatccga aataaaaaac gaaagggatg ggattaatta
     9421 taaatttaag acagcaacaa taataacaac atccagaaag ataaacaaag aggcaacaat
     9481 tacaaatgta tctagcctac taacacaaaa aaggaaaaaa gacatactag aaaacttaaa
     9541 aaaaatagat gacagaattg tagatatagc aatatctgcg ataggtaaca ataaggaaat
     9601 atatttagac attggattta gtgagctaaa tgaaatatca atgcttggcg agggtatcag
     9661 tcgagcatta tcttttatca gctcagtgct ggtacaggaa aactcaatta ttcttattga
     9721 tgaaatcgag aacggaatac attactcagt aattaaagac ataataaaat cattaatttc
     9781 atctgcaaaa caaaacaaca atcaaatatt tgcaacaact catagtcaag atgtgatacg
     9841 agcaataaac gaaatagata gcaaaaatga agatatcgct tatattagac taggaagaga
     9901 aaagaactcc ctaaaaccaa cagccgtaca attcaatatg gatgatttct cctactcagt
     9961 tgagaacgac tgggaggtaa gataatgaaa gacataaata atagcaaaaa gatcattttc
    10021 gttgagggtg atgatgattt tcattttcta tgcaacctac ttgaaataca aggcattacc
    10081 gatgtttttg tcgaaaaaat aaatggaaaa tcaaaattaa aacaagcact tagagcattc
    10141 aaaaacattg attctttcga tgaaaaagaa agtattttta ttatattaga tgctgacaca
    10201 tcattttctg acacagaaag attaataatt gccacattgc gagaactaca aatctcctcc
    10261 cccagctcgc ataacgagat agctatgaat ggcaatacaa aaatttcttt ctttataatg
    10321 ccaggaaaag acaagcaagg tgccattgaa gatctgataa ttgaacacgc aagcactcga
    10381 gcgttatatg aatacataat agaatttttc gaaaaaatta aaaataataa agatgacatc
    10441 tcagcgagtg acaaagagta cacttatcca acaaacgaaa aaaagctaag gtacaagtct
    10501 atcttagctg caatagagaa tccgactcaa gaataggaat atccatgaaa aatggaataa
    10561 taaacacagc agacaattgc tttgatgaaa taaaagattt tatttcaaaa atataaatcc
    10621 aaatcaagcc agcccttcac gggctggctt atcaccaggg tgttattaaa cgccggataa
    10681 cgtatagaaa gcatcataca ctttagttat cagcaatctc aaacgtccta aagacatcac
    10741 cagatgccaa aacaatgatt gtgcagatct aatgtaaaag acccagcaca agatgctccc
    10801 ttaagttata ttctctctat tcacttaaaa caatttaaca taaaatcata attgtacgca
    10861 ccgctaaaac ggactcagga cgtttctgac gggaaaccgg taaaacaggc aacaaaccac
    10921 ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcgca
    10981 agtgcatagc gcggaaattc acaccattca gccaggggaa gtcctgtttc cggcgttctt
    11041 ctcctccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc
    11101 gcagccggaa aaccggtttt cagcaccttc aggaatcggg ttcgtcgacg gccggcaatc
    11161 acgttatggc aagtcgccgg acgactcacc acacgattca ccggcgattc gaatcgtgaa
    11221 ttgaatcaag agtcaccatg agcaaaaaac acccaaagtg atgaattgaa tcaagagtcg
    11281 attcatcata acaatatgat aataaagcgt gattcacata tgaatcgtgg gcaattcgtc
    11341 agcgattcgc catagagtcg tccacgactt ggatctgcac tttgtaacac atcaccgcac
    11401 atttattttt cattaaccca ttgttgtaaa taaggaatct gcaaaatttt aattttgcgt
    11461 ggggtgtggg tatttttagt ggtgaggagc gttcagagat ctgacgattt ttgggaaggt
    11521 tcaggaactc cctgggatgg ccggagagga aaactgaagc atgacggggt caggattttt
    11581 tccagaattt ccaccatggc gccggggatt cagttaattt tgcctggtga ttttcaagaa
    11641 ggagcatggc ctgtttcagg ctgtcaatgt gcgaatcctt ggacaaaagt aaggtgttca
    11701 ggtgttcaat ctcctgtttc agatgacgga tcacctgttc actctggtca gcagaggctg
    11761 tatcacgctg tccagagacc tgtccagaat ggtgttcacc cttcggttgc tcaaaggggc
    11821 caaagacacg taaaagctct gaagtatcta ccatttttgc attgcgctca ccgctacagg
    11881 tggacaggtc acctgcttta attaacctgt gcagtgttgt tctactctta cccgtcagtc
    11941 gggcagcctc actaatagac actctggcca tcagttacca tcctgtaatg catccagtct
    12001 cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcggc
    12061 ctcgacatcg agatcaagct cgttaagctt ctccagcagg agcagagact gattctggat
    12121 gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc ataagaggcc tcagagcgtc
    12181 agtggtcagt gtagggatct cctcttttcc gcttcctcgc tcactcagtc gctacgctcc
    12241 tgccgttcgg ctgcggcgag cggcaggagc atgcgtagaa acgctcgaga aaaccgagta
    12301 atcgcatgga ggaaagaagg aaaggccgca cagcggccgt ttttccatag gctccgcccc
    12361 cctgacgagc atcacggaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
    12421 taaagatacc aggcgtttcc ccctgatggc tccctcgtgc gctctcctgt tcctgccttt
    12481 cggtttaccg gtgtcattcc
//
