LOCUS       NZ_CP137419            12282 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KP101_SE2_14.23_ST147_NDM5_OXA232
            plasmid pKP101_OXA232, complete sequence.
ACCESSION   NZ_CP137419
VERSION     NZ_CP137419.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951958
            Assembly: GCF_033256205.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12282)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 10:15:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,918
            CDSs (total)                      :: 5,794
            Genes (coding)                    :: 5,610
            CDSs (with protein)               :: 5,610
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 88 of 184
            Pseudo Genes (incomplete)         :: 99 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 30 of 184
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137419.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12282
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP101_SE2_14.23_ST147_NDM5_OXA232"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP101_OXA232"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-04"
                     /genotype="ST147"
     gene            join(11612..12282,1..784)
                     /locus_tag="R5O46_RS29530"
                     /old_locus_tag="R5O46_29525"
     CDS             join(11612..12282,1..784)
                     /locus_tag="R5O46_RS29530"
                     /old_locus_tag="R5O46_29525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1066..1347)
                     /locus_tag="R5O46_RS29535"
                     /old_locus_tag="R5O46_29530"
     CDS             complement(1066..1347)
                     /locus_tag="R5O46_RS29535"
                     /old_locus_tag="R5O46_29530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            1691..2488
                     /locus_tag="R5O46_RS29540"
                     /old_locus_tag="R5O46_29535"
     CDS             1691..2488
                     /locus_tag="R5O46_RS29540"
                     /old_locus_tag="R5O46_29535"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(2895..>3047)
                     /locus_tag="R5O46_RS29545"
                     /old_locus_tag="R5O46_29540"
                     /pseudo=""
     CDS             complement(2895..>3047)
                     /locus_tag="R5O46_RS29545"
                     /old_locus_tag="R5O46_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            3423..4370
                     /locus_tag="R5O46_RS29550"
                     /old_locus_tag="R5O46_29545"
     CDS             3423..4370
                     /locus_tag="R5O46_RS29550"
                     /old_locus_tag="R5O46_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            4778..5170
                     /locus_tag="R5O46_RS29555"
                     /old_locus_tag="R5O46_29550"
     CDS             4778..5170
                     /locus_tag="R5O46_RS29555"
                     /old_locus_tag="R5O46_29550"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            5152..5490
                     /locus_tag="R5O46_RS29560"
                     /old_locus_tag="R5O46_29555"
     CDS             5152..5490
                     /locus_tag="R5O46_RS29560"
                     /old_locus_tag="R5O46_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5471..6925
                     /locus_tag="R5O46_RS29565"
                     /old_locus_tag="R5O46_29560"
     CDS             5471..6925
                     /locus_tag="R5O46_RS29565"
                     /old_locus_tag="R5O46_29560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7207..7488)
                     /locus_tag="R5O46_RS29570"
                     /old_locus_tag="R5O46_29565"
     CDS             complement(7207..7488)
                     /locus_tag="R5O46_RS29570"
                     /old_locus_tag="R5O46_29565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            7832..8629
                     /locus_tag="R5O46_RS29575"
                     /old_locus_tag="R5O46_29570"
     CDS             7832..8629
                     /locus_tag="R5O46_RS29575"
                     /old_locus_tag="R5O46_29570"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(9036..>9188)
                     /locus_tag="R5O46_RS29580"
                     /old_locus_tag="R5O46_29575"
                     /pseudo=""
     CDS             complement(9036..>9188)
                     /locus_tag="R5O46_RS29580"
                     /old_locus_tag="R5O46_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            9564..10511
                     /locus_tag="R5O46_RS29585"
                     /old_locus_tag="R5O46_29580"
     CDS             9564..10511
                     /locus_tag="R5O46_RS29585"
                     /old_locus_tag="R5O46_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            10919..11311
                     /locus_tag="R5O46_RS29590"
                     /old_locus_tag="R5O46_29585"
     CDS             10919..11311
                     /locus_tag="R5O46_RS29590"
                     /old_locus_tag="R5O46_29585"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            11293..11631
                     /locus_tag="R5O46_RS29595"
                     /old_locus_tag="R5O46_29590"
     CDS             11293..11631
                     /locus_tag="R5O46_RS29595"
                     /old_locus_tag="R5O46_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             1692..2485
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7833..8626
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3436..4357
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          9577..10498
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3..781
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.25e-158"
     Typing          5472..6922
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11613..12280
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.861"
                     /coverage="100.0"
                     /evalue="2.8599999999999998e-136"
ORIGIN
        1 attaaagggg gtgctgtatg agcaagattt tagacatggc gaaggacttc gagcagagct
       61 cgaagcagca ggccaacgct atcgagaagc gtctgaccga agaattcacg aggcacgaga
      121 aagctatctc cggggcgttg aaatcaagcg aggagaaaat gagcgccgct attgccgagc
      181 acagccagaa aatgggctgg ctgttgctca aaacgtggat ctggatgctg gtcagcatcg
      241 tcctgttctt gagcgcaacg agcgggatcc tctggtatca gggggaattg atagcggagc
      301 ggcaagccac tctccaagcc ttggcagaga agggcggcaa gctaaagctg acccagtgcg
      361 ggaagaaatt ggagcacctt tgcgtgcaga tctccgaccc caatccaacc gtttggggga
      421 aggataccta ttggatcgtg ccgaaggggt actaaatgac gggattggag cgggaattgc
      481 tctgcgcgtt cgaggctttg cagagcgcat acgagcagca gcacaaggca tggcaggaaa
      541 gctacgcgaa cttggcgagc atgtacgaga gcaccaaaca agaggccgag atcagcaggg
      601 cagcagcaga gcgattgacg aggcaggtga acggcttaac gcagcaagtg gacggcttga
      661 gcaagcagct ggagggcttg accgggctag tcagcaagtt gcaaaagaaa gggaacacag
      721 gctcctgaag gcactggatc gaggctggga gcgtggagac agatcgcgag gctttgagcg
      781 ttgacggaac aaaacggccg tcagttataa aaaaaggaaa gtgctatcac tttcctttta
      841 tcggcgggaa ttatcgttga gttcttctat gccatttttc atagtagtaa ctgtaatctt
      901 catctctacc accagattca atccagtcat catactcagg gctatttttg aaatctgaat
      961 cagacatgct aactccttgt tattagttgg cttctatgga agctagcgca ccgtgaagtt
     1021 atgatcaacg atgtagtcca catcattgtc gaaatgtgcg ttcagctagt cttcggtgtt
     1081 ttctgcttta tttctagcgg cgattctcgc gtctgcccat gcggcagact ttttagcctg
     1141 ttctcgttgt tcttcggttg aagactcgct gttgagaact ttgttgctcg tttgtttgac
     1201 caccgaatcc aaacctgttg ttgctgcaaa atgagctagg ttaacaactc ccttacctaa
     1261 caacttggca ctagcgattg cggtttcttt agccccaaaa gcatcagaca cctcctcaag
     1321 cgctttagat aggttaaaaa atcccatact ccccctaaga atatcatcaa taaaattgag
     1381 tgttgctctg tggataactt gcagagttta ttaagtatca ttgcagcaaa gatgaaatca
     1441 atgatttatc aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt gtgagaagca
     1501 gtctaaattc ttcgtgaaat agtgattttt gaagctaata aaaaacacac gtggaattta
     1561 ggtaatagtt aaagcttgtg cctgattgtt gccacaattg gtcagcagtt tgctagggaa
     1621 tgagagggca gagaatgata agcttagcac ttaatttgta tggatatatt gcattaagca
     1681 aggggacgtt atgcgtgtat tagccttatc ggctgtgttt ttggtggcat cgattatcgg
     1741 aatgccagcg gtagcaaagg aatggcaaga aaacaaaagt tggaatgctc actttactga
     1801 acataaatca cagggcgtag ttgtgctctg gaatgagaat aagcagcaag gatttaccaa
     1861 taatcttaaa cgggcgaacc aagcattttt acccgcatct acctttaaaa ttcccaatag
     1921 cttgatcgcc ctcgatttgg gcgtggttaa ggatgaacac caagtcttta agtgggatgg
     1981 acagacgcgt gatatcgccg cttggaatcg tgaccatgac ttaattaccg cgatgaagta
     2041 ctcagttgtg cctgtttatc aagaatttgc ccgccaaatt ggtgaggcac gtatgagtaa
     2101 aatgctgcac gccttcgatt atggcaatga ggatatctcg ggcaatgtag acagtttttg
     2161 gctcgatggt ggtattcgca tttcggctac ccagcaaatc gcttttttac gcaagctgta
     2221 tcacaacaag ctgcacgttt ctgagcgtag tcagcgcatc gtgaaacaag ccatgctgac
     2281 cgaagccaat ggcgactata ttattcgggc taaaacggga tactcgacta gtatcgaacc
     2341 taagattggc tggtgggttg gttgggttga acttgatgat aatgtgtggt tttttgcgat
     2401 gaatatggat atgcccacat cggatggttt agggctgcgc caagccatca caaaagaagt
     2461 gctcaaacag gagaaaatta ttccctagaa gtggttagcg cgtatttgtg tgaaatagcc
     2521 gtcatataag ctgtaaagat atatggacaa aatgctgatg ctcgatgcgc ttgtagttaa
     2581 tggctcatac ttaatcggtc aggcctaatc gacttaagct ccgcgcgtta actttcagcc
     2641 tgagcattag catcacattc ctaaacgtaa aaagcctagc caatttgcta ggcttttttg
     2701 ttatctcaat ggccatggtc actcaacatg ggggtgtgaa taaaggagtg gcgatgtcag
     2761 taaaggtgaa tgccgctgcg ataaagtgcc tagcccatca aggattgtag gctttcttgg
     2821 ctgctaaagc gtttgacgaa aaagtccaag aaggcgctga tattggttct ccatcatttt
     2881 catctaccgg tttactatag gtcaaccagg ccgtccttgc cggcgcttgg gacgcagacg
     2941 atggcatcaa atgcctcgct caaattcgtt tctacagagg cgctttggga ccgcattcgc
     3001 tttgctttca tgggggcatc tgtcaatgtc agacatgaat cctccgatat aggttgattt
     3061 ttgatctctt caacacttgg tgttgtccga ctagagagac ttatatggat cagtattctt
     3121 gcactgacaa tggtgccact caatcccaag gatcgaccta gttagaggcg tagatcaagc
     3181 aattcaaaac agcgcagaag cagaaggagg tggaaatatg ttccagggtg aaaggttaca
     3241 tgcaactaac gttgctctct aggatgtgcc tgctgtaacg tattgtccag aaaagacaaa
     3301 cccccgttat cgcttgcatc ttggcggagc tgcgaaaaac gggggccgtg acgaatcaca
     3361 agagaagtct atcacatgga tcatgccaaa caaccccacg ccgccaataa gcgcggtggt
     3421 gcttgagaaa gtgcgcacag cttgagctgt ttcgggagcg cctgccccgg aaaccctact
     3481 attccgacga gcttacaacg ggcctacgga ttgcggatgt tgcccgcgcc cttggtgcgc
     3541 gttacatcca gccgaacggg ccaacgcacc gccactggat cgtttttgac gtagaccatg
     3601 cagccgctac gttatcttgg gatgacgtag gcgctccagc ccctaacatt accgtcacga
     3661 acaaggccaa cggccacgcg catctgatct acggactgga tacgccgatc cgcactgccc
     3721 cggatggaaa tgcagcccct ctgcggtatg ccgccgccat cgaggccgcg ctccgggaga
     3781 aattaggggc ggatatgggc tactccgggc tgatctgcaa gaaccccctg catgagcact
     3841 ggctggtgca ggtatgggaa ccccggctct acgatttagc ctggctatcc gattacctcg
     3901 atctgagctc ctacaatggc cgcaaatcgc ttcctgagta cgggcttggg cgaaactgta
     3961 ccctgttcga gaaaacgcgc ctgtgggcct ataaagcgat ccggcagggc tggccggatt
     4021 accccgaatg gttggccgct tgtgttgacc gggcatcagg atataacgcc cagttcgagc
     4081 agccgctccc cgcaaacgaa gtccggcaca cggccaagtc cattgccaaa tggacgcacc
     4141 agcacctaag ccccgcaggg ttcagagagg agcaggcccg gagaggtgcg aagggcggca
     4201 aggtatccaa gggaggggga aggccgagca atagcggtaa ggacaagtcc gatctattgc
     4261 cggaggttct gcggcttaag gctcaggggt acaccaaccg cgatatagcg gacgatcttc
     4321 aaatctcccc cagtacggtg agcgtttacc taaagcgtga tcgtccgtga tcgaaacaag
     4381 ccttatcaga taatagcccc ctttaggggg gttcctgcgc tcttgtcttt tagccttaaa
     4441 aaatcctttt agcgcggatc tttggcgagg tggacaactg cgcctgtccg tccatcgccc
     4501 ccctgttttt tcggggggtg tgggcggcag cagttatcca cggagccgct ctttcccttg
     4561 atcttgctct agggcttgca gtatcccgcc catggttgaa taatgtacta agcaaggcta
     4621 cacagaggcc cctctacctg cttcgcggtc tcgggcctct gtggccttgt tcaggggtgc
     4681 tacgcccccc cgaaaccccc tgtggccccc gctcgatccg ccccttcggg acggtctgcg
     4741 cggtggcttc ttggctctgg agaatggacg aggccctatg tcaacaacac agatagagaa
     4801 agattcgaga aggaaggcag tcaatggcgt tatttctagt ttccaactag agggggaggc
     4861 cataaccgat gaattcaaaa ttctacttga agattatatc aatggaaaaa tttccaccga
     4921 tgaggtgtta gctatggtaa gtgacgaacc cacaacaacg gaatacgact acgaaatcac
     4981 cccccgcact ctcggtcttg gcgggggctg gaatctgcgt ttgctggaga acggcgaaga
     5041 ggtcggcggg ggtgttttcc ccttgccgga gcactgcgat tttcgggatg aaaaggcgct
     5101 ccagacgcta ctcgatagcc tctatgagga cgccttagcg gaggcctctg catggctggc
     5161 cagccgttaa agcgcctccg ctctatcaag atccgggtct cagatgcgga gcttgagcgc
     5221 ctgcgggagc tctgccccaa ggcccaactg gcggagtgga tgcgggagca atgtctcggt
     5281 gttgcgcaac cccagcgccg aacgccggcg cctaccgtag atccggcgct cttgcggcag
     5341 ttggccggaa tgggcaacaa cctgaaccag atcgcccggc gcgttaattc cggggagtgg
     5401 gggccgttgg atcggctgcg catcatagcg gagctgtccg ctataggccg cgagctggag
     5461 gaactgcacc atgatcatca aattccataa ccgtggggta ggccggggct ccgggcctgt
     5521 tggctacttg ttgggccgtg acgggaaccg ggaaggcgca acgctcgacc ggggcaaccc
     5581 tgcggtgatt gaagccctga ttgattccag tccctacgcc aaaaaataca cctctggcgt
     5641 tctgtcgttt gccgaggctg atttacccag ggcgaccaag gacaagctga tgagcgactt
     5701 tgaaaaggcg ctcctgcccg gactggaggg caaccagtac gctgttttgt gggtagagca
     5761 ccgcgacaag gggcggctgg agctgaattt tgtagtcccg aacatcgaat tgcagagcgg
     5821 gaagcggctg caaccctact tccacaaggc cgacaatccg cgcctcgatg cgtggcgaac
     5881 cgtggtaaac ggggagctga aattgcacga cccggacgat ccgttaaacc gtcaggccgt
     5941 tatcacgcca aaggatttgc cgagggggca caaggaggcc gctcaggcca ttacagacgg
     6001 gcttttacgc atggccggag caggggagat ccagagccgc gatgatgtgc gcaaagcctt
     6061 gcagggtgcg ggtttcagcg tggtacgcga aaccaaaaac agcctgagca ttgccgaccc
     6121 ggacgggggg cggaacatcc gattaaaggg ggtgctgtat gagcaagatt ttagacatgg
     6181 cgaaggactt cgagcagagc tcgaagcagc aggccaacgc tatcgagaag cgtctgaccg
     6241 aagaattcac gaggcacgag aaagctatct ccggggcgtt gaaatcaagc gaggagaaaa
     6301 tgagcgccgc tattgccgag cacagccaga aaatgggctg gctgttgctc aaaacgtgga
     6361 tctggatgct ggtcagcatc gtcctgttct tgagcgcaac gagcgggatc ctctggtatc
     6421 agggggaatt gatagcggag cggcaagcca ctctccaagc cttggcagag aagggcggca
     6481 agctaaagct gacccagtgc gggaagaaat tggagcacct ttgcgtgcag atctccgacc
     6541 ccaatccaac cgtttggggg aaggatacct attggatcgt gccgaagggg tactaaatga
     6601 cgggattgga gcgggaattg ctctgcgcgt tcgaggcttt gcagagcgca tacgagcagc
     6661 agcacaaggc atggcaggaa agctacgcga acttggcgag catgtacgag agcaccaaac
     6721 aagaggccga gatcagcagg gcagcagcag agcgattgac gaggcaggtg aacggcttaa
     6781 cgcagcaagt ggacggcttg agcaagcagc tggagggctt gaccgggcta gtcagcaagt
     6841 tgcaaaagaa agggaacaca ggctcctgaa ggcactggat cgaggctggg agcgtggaga
     6901 cagatcgcga ggctttgagc gttgacggaa caaaacggcc gtcagttata aaaaaaggaa
     6961 agtgctatca ctttcctttt atcggcggga attatcgttg agttcttcta tgccattttt
     7021 catagtagta actgtaatct tcatctctac caccagattc aatccagtca tcatactcag
     7081 ggctattttt gaaatctgaa tcagacatgc taactccttg ttattagttg gcttctatgg
     7141 aagctagcgc accgtgaagt tatgatcaac gatgtagtcc acatcattgt cgaaatgtgc
     7201 gttcagctag tcttcggtgt tttctgcttt atttctagcg gcgattctcg cgtctgccca
     7261 tgcggcagac tttttagcct gttctcgttg ttcttcggtt gaagactcgc tgttgagaac
     7321 tttgttgctc gtttgtttga ccaccgaatc caaacctgtt gttgctgcaa aatgagctag
     7381 gttaacaact cccttaccta acaacttggc actagcgatt gcggtttctt tagccccaaa
     7441 agcatcagac acctcctcaa gcgctttaga taggttaaaa aatcccatac tccccctaag
     7501 aatatcatca ataaaattga gtgttgctct gtggataact tgcagagttt attaagtatc
     7561 attgcagcaa agatgaaatc aatgatttat caaaaatgat tgaaaggtgg ttgtaaataa
     7621 tgttacaatg tgtgagaagc agtctaaatt cttcgtgaaa tagtgatttt tgaagctaat
     7681 aaaaaacaca cgtggaattt aggtaatagt taaagcttgt gcctgattgt tgccacaatt
     7741 ggtcagcagt ttgctaggga atgagagggc agagaatgat aagcttagca cttaatttgt
     7801 atggatatat tgcattaagc aaggggacgt tatgcgtgta ttagccttat cggctgtgtt
     7861 tttggtggca tcgattatcg gaatgccagc ggtagcaaag gaatggcaag aaaacaaaag
     7921 ttggaatgct cactttactg aacataaatc acagggcgta gttgtgctct ggaatgagaa
     7981 taagcagcaa ggatttacca ataatcttaa acgggcgaac caagcatttt tacccgcatc
     8041 tacctttaaa attcccaata gcttgatcgc cctcgatttg ggcgtggtta aggatgaaca
     8101 ccaagtcttt aagtgggatg gacagacgcg tgatatcgcc gcttggaatc gtgaccatga
     8161 cttaattacc gcgatgaagt actcagttgt gcctgtttat caagaatttg cccgccaaat
     8221 tggtgaggca cgtatgagta aaatgctgca cgccttcgat tatggcaatg aggatatctc
     8281 gggcaatgta gacagttttt ggctcgatgg tggtattcgc atttcggcta cccagcaaat
     8341 cgctttttta cgcaagctgt atcacaacaa gctgcacgtt tctgagcgta gtcagcgcat
     8401 cgtgaaacaa gccatgctga ccgaagccaa tggcgactat attattcggg ctaaaacggg
     8461 atactcgact agtatcgaac ctaagattgg ctggtgggtt ggttgggttg aacttgatga
     8521 taatgtgtgg ttttttgcga tgaatatgga tatgcccaca tcggatggtt tagggctgcg
     8581 ccaagccatc acaaaagaag tgctcaaaca ggagaaaatt attccctaga agtggttagc
     8641 gcgtatttgt gtgaaatagc cgtcatataa gctgtaaaga tatatggaca aaatgctgat
     8701 gctcgatgcg cttgtagtta atggctcata cttaatcggt caggcctaat cgacttaagc
     8761 tccgcgcgtt aactttcagc ctgagcatta gcatcacatt cctaaacgta aaaagcctag
     8821 ccaatttgct aggctttttt gttatctcaa tggccatggt cactcaacat gggggtgtga
     8881 ataaaggagt ggcgatgtca gtaaaggtga atgccgctgc gataaagtgc ctagcccatc
     8941 aaggattgta ggctttcttg gctgctaaag cgtttgacga aaaagtccaa gaaggcgctg
     9001 atattggttc tccatcattt tcatctaccg gtttactata ggtcaaccag gccgtccttg
     9061 ccggcgcttg ggacgcagac gatggcatca aatgcctcgc tcaaattcgt ttctacagag
     9121 gcgctttggg accgcattcg ctttgctttc atgggggcat ctgtcaatgt cagacatgaa
     9181 tcctccgata taggttgatt tttgatctct tcaacacttg gtgttgtccg actagagaga
     9241 cttatatgga tcagtattct tgcactgaca atggtgccac tcaatcccaa ggatcgacct
     9301 agttagaggc gtagatcaag caattcaaaa cagcgcagaa gcagaaggag gtggaaatat
     9361 gttccagggt gaaaggttac atgcaactaa cgttgctctc taggatgtgc ctgctgtaac
     9421 gtattgtcca gaaaagacaa acccccgtta tcgcttgcat cttggcggag ctgcgaaaaa
     9481 cgggggccgt gacgaatcac aagagaagtc tatcacatgg atcatgccaa acaaccccac
     9541 gccgccaata agcgcggtgg tgcttgagaa agtgcgcaca gcttgagctg tttcgggagc
     9601 gcctgccccg gaaaccctac tattccgacg agcttacaac gggcctacgg attgcggatg
     9661 ttgcccgcgc ccttggtgcg cgttacatcc agccgaacgg gccaacgcac cgccactgga
     9721 tcgtttttga cgtagaccat gcagccgcta cgttatcttg ggatgacgta ggcgctccag
     9781 cccctaacat taccgtcacg aacaaggcca acggccacgc gcatctgatc tacggactgg
     9841 atacgccgat ccgcactgcc ccggatggaa atgcagcccc tctgcggtat gccgccgcca
     9901 tcgaggccgc gctccgggag aaattagggg cggatatggg ctactccggg ctgatctgca
     9961 agaaccccct gcatgagcac tggctggtgc aggtatggga accccggctc tacgatttag
    10021 cctggctatc cgattacctc gatctgagct cctacaatgg ccgcaaatcg cttcctgagt
    10081 acgggcttgg gcgaaactgt accctgttcg agaaaacgcg cctgtgggcc tataaagcga
    10141 tccggcaggg ctggccggat taccccgaat ggttggccgc ttgtgttgac cgggcatcag
    10201 gatataacgc ccagttcgag cagccgctcc ccgcaaacga agtccggcac acggccaagt
    10261 ccattgccaa atggacgcac cagcacctaa gccccgcagg gttcagagag gagcaggccc
    10321 ggagaggtgc gaagggcggc aaggtatcca agggaggggg aaggccgagc aatagcggta
    10381 aggacaagtc cgatctattg ccggaggttc tgcggcttaa ggctcagggg tacaccaacc
    10441 gcgatatagc ggacgatctt caaatctccc ccagtacggt gagcgtttac ctaaagcgtg
    10501 atcgtccgtg atcgaaacaa gccttatcag ataatagccc cctttagggg ggttcctgcg
    10561 ctcttgtctt ttagccttaa aaaatccttt tagcgcggat ctttggcgag gtggacaact
    10621 gcgcctgtcc gtccatcgcc cccctgtttt ttcggggggt gtgggcggca gcagttatcc
    10681 acggagccgc tctttccctt gatcttgctc tagggcttgc agtatcccgc ccatggttga
    10741 ataatgtact aagcaaggct acacagaggc ccctctacct gcttcgcggt ctcgggcctc
    10801 tgtggccttg ttcaggggtg ctacgccccc ccgaaacccc ctgtggcccc cgctcgatcc
    10861 gccccttcgg gacggtctgc gcggtggctt cttggctctg gagaatggac gaggccctat
    10921 gtcaacaaca cagatagaga aagattcgag aaggaaggca gtcaatggcg ttatttctag
    10981 tttccaacta gagggggagg ccataaccga tgaattcaaa attctacttg aagattatat
    11041 caatggaaaa atttccaccg atgaggtgtt agctatggta agtgacgaac ccacaacaac
    11101 ggaatacgac tacgaaatca ccccccgcac tctcggtctt ggcgggggct ggaatctgcg
    11161 tttgctggag aacggcgaag aggtcggcgg gggtgttttc cccttgccgg agcactgcga
    11221 ttttcgggat gaaaaggcgc tccagacgct actcgatagc ctctatgagg acgccttagc
    11281 ggaggcctct gcatggctgg ccagccgtta aagcgcctcc gctctatcaa gatccgggtc
    11341 tcagatgcgg agcttgagcg cctgcgggag ctctgcccca aggcccaact ggcggagtgg
    11401 atgcgggagc aatgtctcgg tgttgcgcaa ccccagcgcc gaacgccggc gcctaccgta
    11461 gatccggcgc tcttgcggca gttggccgga atgggcaaca acctgaacca gatcgcccgg
    11521 cgcgttaatt ccggggagtg ggggccgttg gatcggctgc gcatcatagc ggagctgtcc
    11581 gctataggcc gcgagctgga ggaactgcac catgatcatc aaattccata accgtggggt
    11641 aggccggggc tccgggcctg ttggctactt gttgggccgt gacgggaacc gggaaggcgc
    11701 aacgctcgac cggggcaacc ctgcggtgat tgaagccctg attgattcca gtccctacgc
    11761 caaaaaatac acctctggcg ttctgtcgtt tgccgaggct gatttaccca gggcgaccaa
    11821 ggacaagctg atgagcgact ttgaaaaggc gctcctgccc ggactggagg gcaaccagta
    11881 cgctgttttg tgggtagagc accgcgacaa ggggcggctg gagctgaatt ttgtagtccc
    11941 gaacatcgaa ttgcagagcg ggaagcggct gcaaccctac ttccacaagg ccgacaatcc
    12001 gcgcctcgat gcgtggcgaa ccgtggtaaa cggggagctg aaattgcacg acccggacga
    12061 tccgttaaac cgtcaggccg ttatcacgcc aaaggatttg ccgagggggc acaaggaggc
    12121 cgctcaggcc attacagacg ggcttttacg catggccgga gcaggggaga tccagagccg
    12181 cgatgatgtg cgcaaagcct tgcagggtgc gggtttcagc gtggtacgcg aaaccaaaaa
    12241 cagcctgagc attgccgacc cggacggggg gcggaacatc cg
//
