LOCUS       NZ_CP137416            12502 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KP101_SE2_14.23_ST147_NDM5_OXA232
            plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP137416
VERSION     NZ_CP137416.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951958
            Assembly: GCF_033256205.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 10:15:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,918
            CDSs (total)                      :: 5,794
            Genes (coding)                    :: 5,610
            CDSs (with protein)               :: 5,610
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 88 of 184
            Pseudo Genes (incomplete)         :: 99 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 30 of 184
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137416.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP101_SE2_14.23_ST147_NDM5_OXA232"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-04"
                     /genotype="ST147"
     gene            join(11915..12502,1..45)
                     /locus_tag="R5O46_RS01120"
                     /old_locus_tag="R5O46_01120"
     CDS             join(11915..12502,1..45)
                     /locus_tag="R5O46_RS01120"
                     /old_locus_tag="R5O46_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            411..653
                     /locus_tag="R5O46_RS01125"
                     /old_locus_tag="R5O46_01125"
     CDS             411..653
                     /locus_tag="R5O46_RS01125"
                     /old_locus_tag="R5O46_01125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(999..1400)
                     /locus_tag="R5O46_RS01130"
                     /old_locus_tag="R5O46_01130"
     CDS             complement(999..1400)
                     /locus_tag="R5O46_RS01130"
                     /old_locus_tag="R5O46_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1400..1591)
                     /locus_tag="R5O46_RS01135"
                     /old_locus_tag="R5O46_01135"
     CDS             complement(1400..1591)
                     /locus_tag="R5O46_RS01135"
                     /old_locus_tag="R5O46_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2007..2120
                     /locus_tag="R5O46_RS01140"
                     /old_locus_tag="R5O46_01140"
                     /pseudo=""
     CDS             2007..2120
                     /locus_tag="R5O46_RS01140"
                     /old_locus_tag="R5O46_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2465..3580
                     /locus_tag="R5O46_RS01145"
                     /old_locus_tag="R5O46_01145"
     CDS             2465..3580
                     /locus_tag="R5O46_RS01145"
                     /old_locus_tag="R5O46_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            3580..4212
                     /locus_tag="R5O46_RS01150"
                     /old_locus_tag="R5O46_01150"
     CDS             3580..4212
                     /locus_tag="R5O46_RS01150"
                     /old_locus_tag="R5O46_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            4578..4820
                     /locus_tag="R5O46_RS01155"
                     /old_locus_tag="R5O46_01155"
     CDS             4578..4820
                     /locus_tag="R5O46_RS01155"
                     /old_locus_tag="R5O46_01155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(5166..5567)
                     /locus_tag="R5O46_RS01160"
                     /old_locus_tag="R5O46_01160"
     CDS             complement(5166..5567)
                     /locus_tag="R5O46_RS01160"
                     /old_locus_tag="R5O46_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5567..5758)
                     /locus_tag="R5O46_RS01165"
                     /old_locus_tag="R5O46_01165"
     CDS             complement(5567..5758)
                     /locus_tag="R5O46_RS01165"
                     /old_locus_tag="R5O46_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6174..6287
                     /locus_tag="R5O46_RS01170"
                     /old_locus_tag="R5O46_01170"
                     /pseudo=""
     CDS             6174..6287
                     /locus_tag="R5O46_RS01170"
                     /old_locus_tag="R5O46_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            6632..7747
                     /locus_tag="R5O46_RS01175"
                     /old_locus_tag="R5O46_01175"
     CDS             6632..7747
                     /locus_tag="R5O46_RS01175"
                     /old_locus_tag="R5O46_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            7747..8379
                     /locus_tag="R5O46_RS01180"
                     /old_locus_tag="R5O46_01180"
     CDS             7747..8379
                     /locus_tag="R5O46_RS01180"
                     /old_locus_tag="R5O46_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            8745..8987
                     /locus_tag="R5O46_RS01185"
                     /old_locus_tag="R5O46_01185"
     CDS             8745..8987
                     /locus_tag="R5O46_RS01185"
                     /old_locus_tag="R5O46_01185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(9333..9734)
                     /locus_tag="R5O46_RS01190"
                     /old_locus_tag="R5O46_01190"
     CDS             complement(9333..9734)
                     /locus_tag="R5O46_RS01190"
                     /old_locus_tag="R5O46_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9734..9925)
                     /locus_tag="R5O46_RS01195"
                     /old_locus_tag="R5O46_01195"
     CDS             complement(9734..9925)
                     /locus_tag="R5O46_RS01195"
                     /old_locus_tag="R5O46_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            10342..10455
                     /locus_tag="R5O46_RS01200"
                     /old_locus_tag="R5O46_01200"
                     /pseudo=""
     CDS             10342..10455
                     /locus_tag="R5O46_RS01200"
                     /old_locus_tag="R5O46_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            10800..11915
                     /locus_tag="R5O46_RS01205"
                     /old_locus_tag="R5O46_01205"
     CDS             10800..11915
                     /locus_tag="R5O46_RS01205"
                     /old_locus_tag="R5O46_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     Typing          1713..2120
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          5880..6287
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          10048..10455
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gacaattgct ttgatgaaat aaaagatttt atttcaaaaa tataaatcca aatcaagcca
       61 gcccttcacg ggctggctta tcaccagggt gttattaaac gccggataac gtatagaaag
      121 catcatacac tttagttatc agcaatctca aacgtcctaa agacatcacc agatgccaaa
      181 acaatgattg tgcagatcta atgtaaaaga cccagcacaa gatgctccct taagttatat
      241 tctctctatt cacttaaaac aatttaacat aaaatcataa ttgtacgcac cgctaaaacg
      301 gactcaggac gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
      361 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcgcaa gtgcatagcg
      421 cggaaattca caccattcag ccaggggaag tcctgtttcc ggcgttcttc tcctccgaaa
      481 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
      541 accggttttc agcaccttca ggaatcgggt tcgtcgacgg ccggcaatca cgttatggca
      601 agtcgccgga cgactcacca cacgattcac cggcgattcg aatcgtgaat tgaatcaaga
      661 gtcaccatga gcaaaaaaca cccaaagtga tgaattgaat caagagtcga ttcatcataa
      721 caatatgata ataaagcgtg attcacatat gaatcgtggg caattcgtca gcgattcgcc
      781 atagagtcgt ccacgacttg gatctgcact ttgtaacaca tcaccgcaca tttatttttc
      841 attaacccat tgttgtaaat aaggaatctg caaaatttta attttgcgtg gggtgtgggt
      901 atttttagtg gtgaggagcg ttcagagatc tgacgatttt tgggaaggtt caggaactcc
      961 ctgggatggc cggagaggaa aactgaagca tgacggggtc aggatttttt ccagaatttc
     1021 caccatggcg ccggggattc agttaatttt gcctggtgat tttcaagaag gagcatggcc
     1081 tgtttcaggc tgtcaatgtg cgaatccttg gacaaaagta aggtgttcag gtgttcaatc
     1141 tcctgtttca gatgacggat cacctgttca ctctggtcag cagaggctgt atcacgctgt
     1201 ccagagacct gtccagaatg gtgttcaccc ttcggttgct caaaggggcc aaagacacgt
     1261 aaaagctctg aagtatctac catttttgca ttgcgctcac cgctacaggt ggacaggtca
     1321 cctgctttaa ttaacctgtg cagtgttgtt ctactcttac ccgtcagtcg ggcagcctca
     1381 ctaatagaca ctctggccat cagttaccat cctgtaatgc atccagtctc gaggacagtg
     1441 tacggaaaag atgctccgca tcatcgtgga gtttctcaca aaggtcggcc tcgacatcga
     1501 gatcaagctc gttaagcttc tccagcagga gcagagactg attctggatg aatttagcca
     1561 tgttcagcgc tgttttctgt tgtttcgtca taagaggcct cagagcgtca gtggtcagtg
     1621 tagggatctc ctcttttccg cttcctcgct cactcagtcg ctacgctcct gccgttcggc
     1681 tgcggcgagc ggcaggagca tgcgtagaaa cgctcgagaa aaccgagtaa tcgcatggag
     1741 gaaagaagga aaggccgcac agcggccgtt tttccatagg ctccgccccc ctgacgagca
     1801 tcacggaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     1861 ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     1921 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     1981 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg
     2041 cctaatccgg taactatcgt cttgagtcca acccggaaag acacgacaaa gcgccactgg
     2101 cagcagccac tggtaactga tttagggagt tagtcttgaa gttatacgcc agttaaagct
     2161 aaactaaacg aacagttttg gtgactgcgc tccctttaag ccagttacct cggttcatag
     2221 agcgccaact ctactgaacc tttcgaaaaa accgcccgcc aaggcggttt ttttcgttca
     2281 cagccggaag cttacggctg ctgccagata atctcaagaa gaaagtaaaa aaaatcgatt
     2341 aaaacacaag caatcaaagt taatctttga cataaagtct agctaatcac aatgaaagct
     2401 gtacatttta ttaaggtact aacaaatcgt atggcatata acaaaaacac gaacagaggc
     2461 tatcatgata aaggaaataa gtttaaagaa cttcaaatgc ttcaatgagc tatatctaaa
     2521 gcaattaaag actttaaata tcatagctgg gaaaaacaac tacgggaaaa catctatact
     2581 ggatgctatt ttttgttttt atgatgtaaa aaacccggct gttttgttaa acattcaagc
     2641 attcagaaaa gaaatggcag aaataaacaa aaacaagcca ttttgggtta gttactttca
     2701 tgatatggac acatcacaaa agatgtccat agtcattcga gatgaacgct ctgaagtaac
     2761 acaaacatat gaaacagaaa caaatcagag attagaatca agtttgtctc taaacgttga
     2821 caccgtacta tctaaccaaa acatacctcg tcaaactatc ggttctcagg ccacagtaaa
     2881 ctctttaaaa ataaatgtga ccgagacacc caatgcgaaa aacagggttt ccaataagct
     2941 tttctctatg caattgaaag aagatggcgt agaaataaaa tccgaaataa aaaacgaaag
     3001 ggatgggatt aattataaat ttaagacagc aacaataata acaacatcca gaaagataaa
     3061 caaagaggca acaattacaa atgtatctag cctactaaca caaaaaagga aaaaagacat
     3121 actagaaaac ttaaaaaaaa tagatgacag aattgtagat atagcaatat ctgcgatagg
     3181 taacaataag gaaatatatt tagacattgg atttagtgag ctaaatgaaa tatcaatgct
     3241 tggcgagggt atcagtcgag cattatcttt tatcagctca gtgctggtac aggaaaactc
     3301 aattattctt attgatgaaa tcgagaacgg aatacattac tcagtaatta aagacataat
     3361 aaaatcatta atttcatctg caaaacaaaa caacaatcaa atatttgcaa caactcatag
     3421 tcaagatgtg atacgagcaa taaacgaaat agatagcaaa aatgaagata tcgcttatat
     3481 tagactagga agagaaaaga actccctaaa accaacagcc gtacaattca atatggatga
     3541 tttctcctac tcagttgaga acgactggga ggtaagataa tgaaagacat aaataatagc
     3601 aaaaagatca ttttcgttga gggtgatgat gattttcatt ttctatgcaa cctacttgaa
     3661 atacaaggca ttaccgatgt ttttgtcgaa aaaataaatg gaaaatcaaa attaaaacaa
     3721 gcacttagag cattcaaaaa cattgattct ttcgatgaaa aagaaagtat ttttattata
     3781 ttagatgctg acacatcatt ttctgacaca gaaagatcaa taattgccac attgcgagaa
     3841 ctacaaatct cctcccccag ctcgcataac gagatagcta tgaatggcaa tacaaaaatt
     3901 tctttcttta taatgccagg aaaagacaag caaggtgcca ttgaagatct gataattgaa
     3961 cacgcaagca ctcgagcgtt atatgaatac ataatagaat ttttcgaaaa aattaaaaat
     4021 aataaagatg acatctcagc gagtgacaaa gagtacactt atccaacaaa cgaaaaaaaa
     4081 gctaaggtac aagtctatct tagctgcaat agagaatccg actcaagaat aggaatatcc
     4141 atgaaaaatg gaataataaa cacagcagac aattgctttg atgaaataaa agattttatt
     4201 tcaaaaatat aaatccaaat caagccagcc cttcacgggc tggcttatca ccagggtgtt
     4261 attaaacgcc ggataacgta tagaaagcat catacacttt agttatcagc aatctcaaac
     4321 gtcctaaaga catcaccaga tgccaaaaca atgattgtgc agatctaatg taaaagaccc
     4381 agcacaagat gctcccttaa gttatattct ctctattcac ttaaaacaat ttaacataaa
     4441 atcataattg tacgcaccgc taaaacggac tcaggacgtt tctgacggga aaccggtaaa
     4501 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     4561 catgagtatg cgcgcaagtg catagcgcgg aaattcacac cattcagcca ggggaagtcc
     4621 tgtttccggc gttcttctcc tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     4681 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc accttcagga atcgggttcg
     4741 tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg
     4801 cgattcgaat cgtgaattga atcaagagtc accatgagca aaaaacaccc aaagtgatga
     4861 attgaatcaa gagtcgattc atcataacaa tatgataata aagcgtgatt cacatatgaa
     4921 tcgtgggcaa ttcgtcagcg attcgccata gagtcgtcca cgacttggat ctgcactttg
     4981 taacacatca ccgcacattt atttttcatt aacccattgt tgtaaataag gaatctgcaa
     5041 aattttaatt ttgcgtgggg tgtgggtatt tttagtggtg aggagcgttc agagatctga
     5101 cgatttttgg gaaggttcag gaactccctg ggatggccgg agaggaaaac tgaagcatga
     5161 cggggtcagg attttttcca gaatttccac catggcgccg gggattcagt taattttgcc
     5221 tggtgatttt caagaaggag catggcctgt ttcaggctgt caatgtgcga atccttggac
     5281 aaaagtaagg tgttcaggtg ttcaatctcc tgtttcagat gacggatcac ctgttcactc
     5341 tggtcagcag aggctgtatc acgctgtcca gagacctgtc cagaatggtg ttcacccttc
     5401 ggttgctcaa aggggccaaa gacacgtaaa agctctgaag tatctaccat ttttgcattg
     5461 cgctcaccgc tacaggtgga caggtcacct gctttaatta acctgtgcag tgttgttcta
     5521 ctcttacccg tcagtcgggc agcctcacta atagacactc tggccatcag ttaccatcct
     5581 gtaatgcatc cagtctcgag gacagtgtac ggaaaagatg ctccgcatca tcgtggagtt
     5641 tctcacaaag gtcggcctcg acatcgagat caagctcgtt aagcttctcc agcaggagca
     5701 gagactgatt ctggatgaat ttagccatgt tcagcgctgt tttctgttgt ttcgtcataa
     5761 gaggcctcag agcgtcagtg gtcagtgtag ggatctcctc ttttccgctt cctcgctcac
     5821 tcagtcgcta cgctcctgcc gttcggctgc ggcgagcggc aggagcatgc gtagaaacgc
     5881 tcgagaaaac cgagtaatcg catggaggaa agaaggaaag gccgcacagc ggccgttttt
     5941 ccataggctc cgcccccctg acgagcatca cggaatctga cgctcaaatc agtggtggcg
     6001 aaacccgaca ggactataaa gataccaggc gtttccccct gatggctccc tcgtgcgctc
     6061 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     6121 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     6181 aaccccccgt tcagtccgac tgctgcgcct aatccggtaa ctatcgtctt gagtccaacc
     6241 cggaaagaca cgacaaagcg ccactggcag cagccactgg taactgattt agggagttag
     6301 tcttgaagtt atacgccagt taaagctaaa ctaaacgaac agttttggtg actgcgctcc
     6361 ctttaagcca gttacctcgg ttcatagagc gccaactcta ctgaaccttt cgaaaaaacc
     6421 gcccgccaag gcggtttttt tcgttcacag ccggaagctt acggctgctg ccagataatc
     6481 tcaagaagaa agtaaaaaaa atcgattaaa acacaagcaa tcaaagttaa tctttgacat
     6541 aaagtctagc taatcacaat gaaagctgta cattttatta aggtactaac aaatcgtatg
     6601 gcatataaca aaaacacgaa cagaggctat catgataaag gaaataagtt taaagaactt
     6661 caaatgcttc aatgagctat atctaaagca attaaagact ttaaatatca tagctgggaa
     6721 aaacaactac gggaaaacat ctatactgga tgctattttt tgtttttatg atgtaaaaaa
     6781 cccggctgtt ttgttaaaca ttcaagcatt cagaaaagaa atggcagaaa taaacaaaaa
     6841 caagccattt tgggttagtt actttcatga tatggacaca tcacaaaaga tgtccatagt
     6901 cattcgagat gaacgctctg aagtaacaca aacatatgaa acagaaacaa atcagagatt
     6961 agaatcaagt ttgtctctaa acgttgacac cgtactatct aaccaaaaca tacctcgtca
     7021 aactatcggt tctcaggcca cagtaaactc tttaaaaata aatgtgaccg agacacccaa
     7081 tgcgaaaaac agggtttcca ataagctttt ctctatgcaa ttgaaagaag atggcgtaga
     7141 aataaaatcc gaaataaaaa acgaaaggga tgggattaat tataaattta agacagcaac
     7201 aataataaca acatccagaa agataaacaa agaggcaaca attacaaatg tatctagcct
     7261 actaacacaa aaaaggaaaa aagacatact agaaaactta aaaaaaatag atgacagaat
     7321 tgtagatata gcaatatctg cgataggtaa caataaggaa atatatttag acattggatt
     7381 tagtgagcta aatgaaatat caatgcttgg cgagggtatc agtcgagcat tatcttttat
     7441 cagctcagtg ctggtacagg aaaactcaat tattcttatt gatgaaatcg agaacggaat
     7501 acattactca gtaattaaag acataataaa atcattaatt tcatctgcaa aacaaaacaa
     7561 caatcaaata tttgcaacaa ctcatagtca agatgtgata cgagcaataa acgaaataga
     7621 tagcaaaaat gaagatatcg cttatattag actaggaaga gaaaagaact ccctaaaacc
     7681 aacagccgta caattcaata tggatgattt ctcctactca gttgagaacg actgggaggt
     7741 aagataatga aagacataaa taatagcaaa aagatcattt tcgttgaggg tgatgatgat
     7801 tttcattttc tatgcaacct acttgaaata caaggcatta ccgatgtttt tgtcgaaaaa
     7861 ataaatggaa aatcaaaatt aaaacaagca cttagagcat tcaaaaacat tgattctttc
     7921 gatgaaaaag aaagtatttt tattatatta gatgctgaca catcattttc tgacacagaa
     7981 agatcaataa ttgccacatt gcgagaacta caaatctcct cccccagctc gcataacgag
     8041 atagctatga atggcaatac aaaaatttct ttctttataa tgccaggaaa agacaagcaa
     8101 ggtgccattg aagatctgat aattgaacac gcaagcactc gagcgttata tgaatacata
     8161 atagaatttt tcgaaaaaat taaaaataat aaagatgaca tctcagcgag tgacaaagag
     8221 tacacttatc caacaaacga aaaaaaagct aaggtacaag tctatcttag ctgcaataga
     8281 gaatccgact caagaatagg aatatccatg aaaaatggaa taataaacac agcagacaat
     8341 tgctttgatg aaataaaaga ttttatttca aaaatataaa tccaaatcaa gccagccctt
     8401 cacgggctgg cttatcacca gggtgttatt aaacgccgga taacgtatag aaagcatcat
     8461 acactttagt tatcagcaat ctcaaacgtc ctaaagacat caccagatgc caaaacaatg
     8521 attgtgcaga tctaatgtaa aagacccagc acaagatgct cccttaagtt atattctctc
     8581 tattcactta aaacaattta acataaaatc ataattgtac gcaccgctaa aacggactca
     8641 ggacgtttct gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa
     8701 acgcgcaggc tctgttttta cggcatgcat gagtatgcgc gcaagtgcat agcgcggaaa
     8761 ttcacaccat tcagccaggg gaagtcctgt ttccggcgtt cttctcctcc gaaaacggtg
     8821 aacggggacg caatttattg cgtggccgtg aagcgttgcc ggcgcagccg gaaaaccggt
     8881 tttcagcacc ttcaggaatc gggttcgtcg acggccggca atcacgttat ggcaagtcgc
     8941 cggacgactc accacacgat tcaccggcga ttcgaatcgt gaattgaatc aagagtcacc
     9001 atgagcaaaa aacacccaaa gtgatgaatt gaatcaagag tcgattcatc ataacaatat
     9061 gataataaag cgtgattcac atatgaatcg tgggcaattc gtcagcgatt cgccatagag
     9121 tcgtccacga cttggatctg cactttgtaa cacatcaccg cacatttatt tttcattaac
     9181 ccattgttgt aaataaggaa tctgcaaaat tttaattttg cgtggggtgt gggtattttt
     9241 agtggtgagg agcgttcaga gatctgacga tttttgggaa ggttcaggaa ctccctggga
     9301 tggccggaga ggaaaactga agcatgacgg ggtcaggatt ttttccagaa tttccaccat
     9361 ggcgccgggg attcagttaa ttttgcctgg tgattttcaa gaaggagcat ggcctgtttc
     9421 aggctgtcaa tgtgcgaatc cttggacaaa agtaaggtgt tcaggtgttc aatctcctgt
     9481 ttcagatgac ggatcacctg ttcactctgg tcagcagagg ctgtatcacg ctgtccagag
     9541 acctgtccag aatggtgttc acccttcggt tgctcaaagg ggccaaagac acgtaaaagc
     9601 tctgaagtat ctaccatttt tgcattgcgc tcaccgctac aggtggacag gtcacctgct
     9661 ttaattaacc tgtgcagtgt tgttctactc ttacccgtca gtcgggcagc ctcactaata
     9721 gacactctgg ccatcagtta ccatcctgta atgcatccag tctcgaggac agtgtacgga
     9781 aaagatgctc cgcatcatcg tggagtttct cacaaaggtc ggcctcgaca tcgagatcaa
     9841 gctcgttaag cttctccagc aggagcagag actgattctg gatgaattta gccatgttca
     9901 gcgctgtttt ctgttgtttc gtcataagag gcctcagagc gtcagtggtc agtgtaggga
     9961 tctcctcttt ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt tcggctgcgg
    10021 cgagcggcag gagcatgcgt agaaacgctc gagaaaaccg agtaatcgca tggaggaaag
    10081 aaggaaaggc cgcacagcgg ccgtttttcc ataggctccg cccccctgac gagcatcacg
    10141 gaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
    10201 ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
    10261 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
    10321 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctaa
    10381 tccggtaact atcgtcttga gtccaacccg gaaagacacg acaaagcgcc actggcagca
    10441 gccactggta actgatttag ggagttagtc ttgaagttat acgccagtta aagctaaact
    10501 aaacgaacag ttttggtgac tgcgctccct ttaagccagt tacctcggtt catagagcgc
    10561 caactctact gaacctttcg aaaaaaccgc ccgccaaggc ggtttttttc gttcacagcc
    10621 ggaagcttac ggctgctgcc agataatctc aagaagaaag taaaaaaaat cgattaaaac
    10681 acaagcaatc aaagttaatc tttgacataa agtctagcta atcacaatga aagctgtaca
    10741 ttttattaag gtactaacaa atcgtatggc atataacaaa aacacgaaca gaggctatca
    10801 tgataaagga aataagttta aagaacttca aatgcttcaa tgagctatat ctaaagcaat
    10861 taaagacttt aaatatcata gctgggaaaa acaactacgg gaaaacatct atactggatg
    10921 ctattttttg tttttatgat gtaaaaaacc cggctgtttt gttaaacatt caagcattca
    10981 gaaaagaaat ggcagaaata aacaaaaaca agccattttg ggttagttac tttcatgata
    11041 tggacacatc acaaaagatg tccatagtca ttcgagatga acgctctgaa gtaacacaaa
    11101 catatgaaac agaaacaaat cagagattag aatcaagttt gtctctaaac gttgacaccg
    11161 tactatctaa ccaaaacata cctcgtcaaa ctatcggttc tcaggccaca gtaaactctt
    11221 taaaaataaa tgtgaccgag acacccaatg cgaaaaacag ggtttccaat aagcttttct
    11281 ctatgcaatt gaaagaagat ggcgtagaaa taaaatccga aataaaaaac gaaagggatg
    11341 ggattaatta taaatttaag acagcaacaa taataacaac atccagaaag ataaacaaag
    11401 aggcaacaat tacaaatgta tctagcctac taacacaaaa aaggaaaaaa gacatactag
    11461 aaaacttaaa aaaaatagat gacagaattg tagatatagc aatatctgcg ataggtaaca
    11521 ataaggaaat atatttagac attggattta gtgagctaaa tgaaatatca atgcttggcg
    11581 agggtatcag tcgagcatta tcttttatca gctcagtgct ggtacaggaa aactcaatta
    11641 ttcttattga tgaaatcgag aacggaatac attactcagt aattaaagac ataataaaat
    11701 cattaatttc atctgcaaaa caaaacaaca atcaaatatt tgcaacaact catagtcaag
    11761 atgtgatacg agcaataaac gaaatagata gcaaaaatga agatatcgct tatattagac
    11821 taggaagaga aaagaactcc ctaaaaccaa cagccgtaca attcaatatg gatgatttct
    11881 cctactcagt tgagaacgac tgggaggtaa gataatgaaa gacataaata atagcaaaaa
    11941 gatcattttc gttgagggtg atgatgattt tcattttcta tgcaacctac ttgaaataca
    12001 aggcattacc gatgtttttg tcgaaaaaat aaatggaaaa tcaaaattaa aacaagcact
    12061 tagagcattc aaaaacattg attctttcga tgaaaaagaa agtattttta ttatattaga
    12121 tgctgacaca tcattttctg acacagaaag atcaataatt gccacattgc gagaactaca
    12181 aatctcctcc cccagctcgc ataacgagat agctatgaat ggcaatacaa aaatttcttt
    12241 ctttataatg ccaggaaaag acaagcaagg tgccattgaa gatctgataa ttgaacacgc
    12301 aagcactcga gcgttatatg aatacataat agaatttttc gaaaaaatta aaaataataa
    12361 agatgacatc tcagcgagtg acaaagagta cacttatcca acaaacgaaa aaaaagctaa
    12421 ggtacaagtc tatcttagct gcaatagaga atccgactca agaataggaa tatccatgaa
    12481 aaatggaata ataaacacag ca
//
