LOCUS       NZ_CP137412             4167 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP102_WM3_17.23_ST147_OXA181 plasmid
            unnamed5, complete sequence.
ACCESSION   NZ_CP137412
VERSION     NZ_CP137412.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951959
            Assembly: GCF_033255445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:43:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,612
            Genes (coding)                    :: 5,457
            CDSs (with protein)               :: 5,457
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 86 of 155
            Pseudo Genes (incomplete)         :: 68 of 155
            Pseudo Genes (internal stop)      :: 21 of 155
            Pseudo Genes (multiple problems)  :: 17 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137412.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP102_WM3_17.23_ST147_OXA181"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-04"
                     /genotype="ST147"
     gene            complement(105..347)
                     /locus_tag="R5O47_RS28630"
                     /old_locus_tag="R5O47_28630"
     CDS             complement(105..347)
                     /locus_tag="R5O47_RS28630"
                     /old_locus_tag="R5O47_28630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(713..1345)
                     /locus_tag="R5O47_RS28635"
                     /old_locus_tag="R5O47_28635"
     CDS             complement(713..1345)
                     /locus_tag="R5O47_RS28635"
                     /old_locus_tag="R5O47_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1345..2460)
                     /locus_tag="R5O47_RS28640"
                     /old_locus_tag="R5O47_28640"
     CDS             complement(1345..2460)
                     /locus_tag="R5O47_RS28640"
                     /old_locus_tag="R5O47_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2805..2918)
                     /locus_tag="R5O47_RS28645"
                     /old_locus_tag="R5O47_28645"
                     /pseudo=""
     CDS             complement(2805..2918)
                     /locus_tag="R5O47_RS28645"
                     /old_locus_tag="R5O47_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3334..3525
                     /locus_tag="R5O47_RS28650"
                     /old_locus_tag="R5O47_28650"
     CDS             3334..3525
                     /locus_tag="R5O47_RS28650"
                     /old_locus_tag="R5O47_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3525..3926
                     /locus_tag="R5O47_RS28655"
                     /old_locus_tag="R5O47_28655"
     CDS             3525..3926
                     /locus_tag="R5O47_RS28655"
                     /old_locus_tag="R5O47_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2806..3213)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 acgattcata tgtgaatcac gctttattat catattgtta tgatgaatcg actcttgatt
       61 caattcatca ctttgggtgt tttttgctca tggtgactct tgattcaatt cacgattcga
      121 atcgccggtg aatcgtgtgg tgagtcgtcc ggcgacttgc cataacgtga ttgccggccg
      181 tcgacgaacc cgattcctga aggtgctgaa aaccggtttt ccggctgcgc cggcaacgct
      241 tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggaggagaa gaacgccgga
      301 aacaggactt cccctggctg aatggtgtga atttccgcgc tatgcacttg cgcgcatact
      361 catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc
      421 ctgttttacc ggtttcccgt cagaaacgtc ctgagtccgt tttagcggtg cgtacaatta
      481 tgattttatg ttaaattgtt ttaagtgaat agagagaata taacttaagg gagcatcttg
      541 tgctgggtct tttacattag atctgcacaa tcattgtttt ggcatctggt gatgtcttta
      601 ggacgtttga gattgctgat aactaaagtg tatgatgctt tctatacgtt atccggcgtt
      661 taataacacc ctggtgataa gccagcccgt gaagggctgg cttgatttgg atttatattt
      721 ttgaaataaa atcttttatt tcatcaaagc aattgtctgc tgtgtttatt attccatttt
      781 tcatggatat tcctattctt gagtcggatt ctctattgca gctaagatag acttgtacct
      841 tagctttttt ttcgtttgtt ggataagtgt actctttgtc actcgctgag atgtcatctt
      901 tattattttt aattttttcg aaaaattcta ttatgtattc atataacgct cgagtgcttg
      961 cgtgttcaat tatcagatct tcaatggcac cttgcttgtc ttttcctggc attataaaga
     1021 aagaaatttt tgtattgcca ttcatagcta tctcgttatg cgagctgggg gaggagattt
     1081 gtagttctcg caatgtggca attattgatc tttctgtgtc agaaaatgat gtgtcagcat
     1141 ctaatataat aaaaatactt tctttttcat cgaaagaatc aatgtttttg aatgctctaa
     1201 gtgcttgttt taattttgat tttccattta ttttttcgac aaaaacatcg gtaatgcctt
     1261 gtatttcaag taggttgcat agaaaatgaa aatcatcatc accctcaacg aaaatgatct
     1321 ttttgctatt atttatgtct ttcattatct tacctcccag tcgttctcaa ctgagtagga
     1381 gaaatcatcc atattgaatt gtacggctgt tggttttagg gagttctttt ctcttcctag
     1441 tctaatataa gcgatatctt catttttgct atctatttcg tttattgctc gtatcacatc
     1501 ttgactatga gttgttgcaa atatttgatt gttgttttgt tttgcagatg aaattaatga
     1561 ttttattatg tctttaatta ctgagtaatg tattccgttc tcgatttcat caataagaat
     1621 aattgagttt tcctgtacca gcactgagct gataaaagat aatgctcgac tgataccctc
     1681 gccaagcatt gatatttcat ttagctcact aaatccaatg tctaaatata tttccttatt
     1741 gttacctatc gcagatattg ctatatctac aattctgtca tctatttttt ttaagttttc
     1801 tagtatgtct tttttccttt tttgtgttag taggctagat acatttgtaa ttgttgcctc
     1861 tttgtttatc tttctggatg ttgttattat tgttgctgtc ttaaatttat aattaatccc
     1921 atccctttcg ttttttattt cggattttat ttctacgcca tcttctttca attgcataga
     1981 gaaaagctta ttggaaaccc tgtttttcgc attgggtgtc tcggtcacat ttatttttaa
     2041 agagtttact gtggcctgag aaccgatagt ttgacgaggt atgttttggt tagatagtac
     2101 ggtgtcaacg tttagagaca aacttgattc taatctctga tttgtttctg tttcatatgt
     2161 ttgtgttact tcagagcgtt catctcgaat gactatggac atcttttgtg atgtgtccat
     2221 atcatgaaag taactaaccc aaaatggctt gtttttgttt atttctgcca tttcttttct
     2281 gaatgcttga atgtttaaca aaacagccgg gttttttaca tcataaaaac aaaaaatagc
     2341 atccagtata gatgttttcc cgtagttgtt tttcccagct atgatattta aagtctttaa
     2401 ttgctttaga tatagctcat tgaagcattt gaagttcttt aaacttattt cctttatcat
     2461 gatagcctct gttcgtgttt ttgttatatg ccatacgatt tgttagtacc ttaataaaat
     2521 gtacagcttt cattgtgatt agctagactt tatgtcaaag attaactttg attgcttgtg
     2581 ttttaatcga ttttttttac tttcttcttg agattatctg gcagcagccg taagcttccg
     2641 gctgtgaacg aaaaaaaccg ccttggcggg cggttttttc gaaaggttca gtagagttgg
     2701 cgctctatga accgaggtaa ctggcttaaa gggagcgcag tcaccaaaac tgttcgttta
     2761 gtttagcttt aactggcgta taacttcaag actaactccc taaatcagtt accagtggct
     2821 gctgccagtg gcgctttgtc gtgtctttcc gggttggact caagacgata gttaccggat
     2881 taggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
     2941 gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat
     3001 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccat cagggggaaa
     3061 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagattcc
     3121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacggc cgctgtgcgg cctttccttc
     3181 tttcctccat gcgattactc ggttttctcg agcgtttcta cgcatgctcc tgccgctcgc
     3241 cgcagccgaa cggcaggagc gtagcgactg agtgagcgag gaagcggaaa agaggagatc
     3301 cctacactga ccactgacgc tctgaggcct cttatgacga aacaacagaa aacagcgctg
     3361 aacatggcta aattcatcca gaatcagtct ctgctcctgc tggagaagct taacgagctt
     3421 gatctcgatg tcgaggccga cctttgtgag aaactccacg atgatgcgga gcatcttttc
     3481 cgtacactgt cctcgagact ggatgcatta caggatggta actgatggcc agagtgtcta
     3541 ttagtgaggc tgcccgactg acgggtaaga gtagaacaac actgcacagg ttaattaaag
     3601 caggtgacct gtccacctgt agcggtgagc gcaatgcaaa aatggtagat acttcagagc
     3661 ttttacgtgt ctttggcccc tttgagcaac cgaagggtga acaccattct ggacaggtct
     3721 ctggacagcg tgatacagcc tctgctgacc agagtgaaca ggtgatccgt catctgaaac
     3781 aggagattga acacctgaac accttacttt tgtccaagga ttcgcacatt gacagcctga
     3841 aacaggccat gctccttctt gaaaatcacc aggcaaaatt aactgaatcc ccggcgccat
     3901 ggtggaaatt ctggaaaaaa tcctgacccc gtcatgcttc agttttcctc tccggccatc
     3961 ccagggagtt cctgaacctt cccaaaaatc gtcagatctc tgaacgctcc tcaccactaa
     4021 aaatacccac accccacgca aaattaaaat tttgcagatt ccttatttac aacaatgggt
     4081 taatgaaaaa taaatgtgcg gtgatgtgtt acaaagtgca gatccaagtc gtggacgact
     4141 ctatggcgaa tcgctgacga attgccc
//
