LOCUS       NZ_CP137409            12206 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP102_WM3_17.23_ST147_OXA181 plasmid
            pKP102_OXA181, complete sequence.
ACCESSION   NZ_CP137409
VERSION     NZ_CP137409.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951959
            Assembly: GCF_033255445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12206)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12206)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:43:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,612
            Genes (coding)                    :: 5,457
            CDSs (with protein)               :: 5,457
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 86 of 155
            Pseudo Genes (incomplete)         :: 68 of 155
            Pseudo Genes (internal stop)      :: 21 of 155
            Pseudo Genes (multiple problems)  :: 17 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137409.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12206
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP102_WM3_17.23_ST147_OXA181"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP102_OXA181"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-04"
                     /genotype="ST147"
     gene            complement(join(12190..12206,1..781))
                     /locus_tag="R5O47_RS26940"
                     /old_locus_tag="R5O47_26935"
     CDS             complement(join(12190..12206,1..781))
                     /locus_tag="R5O47_RS26940"
                     /old_locus_tag="R5O47_26935"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /protein_id="WP_015060052.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
     gene            1087..1368
                     /locus_tag="R5O47_RS26945"
                     /old_locus_tag="R5O47_26940"
     CDS             1087..1368
                     /locus_tag="R5O47_RS26945"
                     /old_locus_tag="R5O47_26940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(1650..3104)
                     /locus_tag="R5O47_RS26950"
                     /old_locus_tag="R5O47_26945"
     CDS             complement(1650..3104)
                     /locus_tag="R5O47_RS26950"
                     /old_locus_tag="R5O47_26945"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3085..3423)
                     /locus_tag="R5O47_RS26955"
                     /old_locus_tag="R5O47_26950"
     CDS             complement(3085..3423)
                     /locus_tag="R5O47_RS26955"
                     /old_locus_tag="R5O47_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3405..3797)
                     /locus_tag="R5O47_RS26960"
                     /old_locus_tag="R5O47_26955"
     CDS             complement(3405..3797)
                     /locus_tag="R5O47_RS26960"
                     /old_locus_tag="R5O47_26955"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(4205..5152)
                     /locus_tag="R5O47_RS26965"
                     /old_locus_tag="R5O47_26960"
     CDS             complement(4205..5152)
                     /locus_tag="R5O47_RS26965"
                     /old_locus_tag="R5O47_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            <5528..5680
                     /locus_tag="R5O47_RS26970"
                     /old_locus_tag="R5O47_26965"
                     /pseudo=""
     CDS             <5528..5680
                     /locus_tag="R5O47_RS26970"
                     /old_locus_tag="R5O47_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(6087..6884)
                     /locus_tag="R5O47_RS26975"
                     /old_locus_tag="R5O47_26970"
     CDS             complement(6087..6884)
                     /locus_tag="R5O47_RS26975"
                     /old_locus_tag="R5O47_26970"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /protein_id="WP_015060052.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
     gene            7190..7471
                     /locus_tag="R5O47_RS26980"
                     /old_locus_tag="R5O47_26975"
     CDS             7190..7471
                     /locus_tag="R5O47_RS26980"
                     /old_locus_tag="R5O47_26975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(7753..9207)
                     /locus_tag="R5O47_RS26985"
                     /old_locus_tag="R5O47_26980"
     CDS             complement(7753..9207)
                     /locus_tag="R5O47_RS26985"
                     /old_locus_tag="R5O47_26980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9188..9526)
                     /locus_tag="R5O47_RS26990"
                     /old_locus_tag="R5O47_26985"
     CDS             complement(9188..9526)
                     /locus_tag="R5O47_RS26990"
                     /old_locus_tag="R5O47_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(9508..9900)
                     /locus_tag="R5O47_RS26995"
                     /old_locus_tag="R5O47_26990"
     CDS             complement(9508..9900)
                     /locus_tag="R5O47_RS26995"
                     /old_locus_tag="R5O47_26990"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(10308..11255)
                     /locus_tag="R5O47_RS27000"
                     /old_locus_tag="R5O47_26995"
     CDS             complement(10308..11255)
                     /locus_tag="R5O47_RS27000"
                     /old_locus_tag="R5O47_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            <11631..11783
                     /locus_tag="R5O47_RS27005"
                     /old_locus_tag="R5O47_27000"
                     /pseudo=""
     CDS             <11631..11783
                     /locus_tag="R5O47_RS27005"
                     /old_locus_tag="R5O47_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             complement(6091..6884)
                     /name="blaOXA-181"
                     /gene="blaOXA-181"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3..781)
                     /name="blaOXA"
                     /gene="blaOXA"
                     /gene_name="OXA-48 family class D beta-lactamase"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="98.11"
     ARG             complement(3..781)
                     /name="OXA-181"
                     /gene="OXA-181"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="98.11"
     Typing          complement(4219..5140)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(10322..11243)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(1654..3104)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7757..9207)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctgtttgag cacttctttt gtgatggctt ggcgcagccc taaaccatcc gatgtgggca
       61 tatccatatt catcgcaaaa aaccacacat tatcatcaag ttcaacccaa ccaacccacc
      121 agccaatctt aggttcgatt ctagtcgagt atcccgtttt agcccgaata atatagtcgc
      181 cattggcttc ggtcagcatg gcttgtttca cgatgcgctg actacgctca gaaacgtgca
      241 gcttgttgtg atacagcttg cgtaaaaaag cgatttgctg ggtagccgaa atgcgaatac
      301 caccatcgag ccaaaaactg tctacattgc ccgagatatc ctcattgcca taatcgaagg
      361 cgtgcagcat tttactcata cgtgcctcac caatttggcg ggcaaattct tgataaacag
      421 gcacaactga gtacttcatc gcggtaatta agtcatggtc acgattccaa gcggcgatat
      481 cacgcgtctg tccatcccac ttaaagactt ggtgttcatc cttaaccacg cccaaatcga
      541 gggcgatcaa gctattggga attttaaagg tagatgcggg taaaaatgct tggttcgccc
      601 gtttaagatt attggtaaat ccttgctgct tattctcatt ccagagcaca actacgccct
      661 gtgatttatg ttcagtaaag tgagcattcc aacttttgtt ttcttgccat tcctttgcta
      721 ccgctggcat tccgataatc gatgccacca aaaacacagc cgataaggct aatacacgca
      781 taacgtcccc ttgcttaatg caatatatcc atacaaatta agtgctaagc ttatcattct
      841 ctgccctctc attccctagc aaactgctga ccaattgtgg caacaatcag gcacaagctt
      901 taactattac ctaaattcca cgtgtgtttt ttattagctt caaaaatcac tatttcacga
      961 agaatttaga ctgcttctca cacattgtaa cattatttac aaccaccttt caatcatttt
     1021 tgataaatca ttgatttcat ctttgctgca atgatactta ataaactctg caagttatcg
     1081 gggagtatgg gattttttaa cctatctaaa gcgcttgagg aggtgtctga tgcttttggg
     1141 gctaaagaaa ccgcaatcgc tagtgccaag ttgttaggta agggagttgt taacctagct
     1201 cattttgcag caacaacagg tttggattcg gtggtcaaac aaacgagcaa caaagttctc
     1261 aacagcgagt cttcaaccga agaacaacga gaacaggcta aaaagtctgc cgcatgggca
     1321 gacgcgagaa tcgccgctag aaataaagca gaaaacaccg aagactagct gaacgcacat
     1381 ttcgacaatg atgtggacta catcgttgat cataacttca cggtgcgcta gcttccatag
     1441 aagccaacta ataacaagga gttagcatgt ctgattcaga tttcaaaaat agccctgagt
     1501 atgatgactg gattgaatct ggtggtagag atgaagatta cagttactac tatgaaaaat
     1561 ggcatagaag aactcaacga taattcccgc cgataaaagg aaagtgatag cactttcctt
     1621 tttttataac tgacggccgt tttgttccgt caacgctcaa agcctcgcga tctgtctcca
     1681 cgctcccagc ctcgatccag tgccttcagg agcctgtgtt ccctttcttt tgcaacttgc
     1741 tgactagccc ggtcaagccc tccagctgct tgctcaagcc gtccacttgc tgcgttaagc
     1801 cgttcacctg cctcgtcaat cgctctgctg ctgccctgct gatctcggcc tcttgtttgg
     1861 tgctctcgta catgctcgcc aagttcgcgt agctttcctg ccatgccttg tgctgctgct
     1921 cgtatgcgct ctgcaaagcc tcgaacgcgc agagcaattc ccgctccaat cccgtcattt
     1981 agtacccctt cggcacgatc caataggtat ccttccccca aacggttgga ttggggtcgg
     2041 agatctgcac gcaaaggtgc tccaatttct tcccgcactg ggtcagcttt agcttgccgc
     2101 ccttctctgc caaggcttgg agagtggctt gccgctccgc tatcaattcc ccctgatacc
     2161 agaggatccc gctcgttgcg ctcaagaaca ggacgatgct gaccagcatc cagatccacg
     2221 ttttgagcaa cagccagccc attttctggc tgtgctcggc aatagcggcg ctcattttct
     2281 cctcgcttga tttcaacgcc ccggagatag ctttctcgtg cctcgtgaat tcttcggtca
     2341 gacgcttctc gatagcgttg gcctgctgct tcgagctctg ctcgaagtcc ttcgccatgt
     2401 ctaaaatctt gctcatacag cacccccttt aatcggatgt tccgcccccc gtccgggtcg
     2461 gcaatgctca ggctgttttt ggtttcgcgt accacgctga aacccgcacc ctgcaaggct
     2521 ttgcgcacat catcgcggct ctggatctcc cctgctccgg ccatgcgtaa aagcccgtct
     2581 gtaatggcct gagcggcctc cttgtgcccc ctcggcaaat cctttggcgt gataacggcc
     2641 tgacggttta acggatcgtc cgggtcgtgc aatttcagct ccccgtttac cacggttcgc
     2701 cacgcatcga ggcgcggatt gtcggccttg tggaagtagg gttgcagccg cttcccgctc
     2761 tgcaattcga tgttcgggac tacaaaattc agctccagcc gccccttgtc gcggtgctct
     2821 acccacaaaa cagcgtactg gttgccctcc agtccgggca ggagcgcctt ttcaaagtcg
     2881 ctcatcagct tgtccttggt cgccctgggt aaatcagcct cggcaaacga cagaacgcca
     2941 gaggtgtatt ttttggcgta gggactggaa tcaatcaggg cttcaatcac cgcagggttg
     3001 ccccggtcga gcgttgcgcc ttcccggttc ccgtcacggc ccaacaagta gccaacaggc
     3061 ccggagcccc ggcctacccc acggttatgg aatttgatga tcatggtgca gttcctccag
     3121 ctcgcggcct atagcggaca gctccgctat gatgcgcagc cgatccaacg gcccccactc
     3181 cccggaatta acgcgccggg cgatctggtt caggttgttg cccattccgg ccaactgccg
     3241 caagagcgcc ggatctacgg taggcgccgg cgttcggcgc tggggttgcg caacaccgag
     3301 acattgctcc cgcatccact ccgccagttg ggccttgggg cagagctccc gcaggcgctc
     3361 aagctccgca tctgagaccc ggatcttgat agagcggagg cgctttaacg gctggccagc
     3421 catgcagagg cctccgctaa ggcgtcctca tagaggctat cgagtagcgt ctggagcgcc
     3481 ttttcatccc gaaaatcgca gtgctccggc aaggggaaaa cacccccgcc gacctcttcg
     3541 ccgttctcca gcaaacgcag attccagccc ccgccaagac cgagagtgcg gggggtgatt
     3601 tcgtagtcgt attccgttgt tgtgggttcg tcacttacca tagctaacac ctcatcggtg
     3661 gaaatttttc cattgatata atcttcaagt agaattttga attcatcggt tatggcctcc
     3721 ccctctagtt ggaaactaga aataacgcca ttgactgcct tccttctcga atctttctct
     3781 atctgtgttg ttgacatagg gcctcgtcca ttctccagag ccaagaagcc accgcgcaga
     3841 ccgtcccgaa ggggcggatc gagcgggggc cacagggggt ttcggggggg cgtagcaccc
     3901 ctgaacaagg ccacagaggc ccgagaccgc gaagcaggta gaggggcctc tgtgtagcct
     3961 tgcttagtac attattcaac catgggcggg atactgcaag ccctagagca agatcaaggg
     4021 aaagagcggc tccgtggata actgctgccg cccacacccc ccgaaaaaac aggggggcga
     4081 tggacggaca ggcgcagttg tccacctcgc caaagatccg cgctaaaagg attttttaag
     4141 gctaaaagac aagagcgcag gaacccccct aaagggggct attatctgat aaggcttgtt
     4201 tcgatcacgg acgatcacgc tttaggtaaa cgctcaccgt actgggggag atttgaagat
     4261 cgtccgctat atcgcggttg gtgtacccct gagccttaag ccgcagaacc tccggcaata
     4321 gatcggactt gtccttaccg ctattgctcg gccttccccc tcccttggat accttgccgc
     4381 ccttcgcacc tctccgggcc tgctcctctc tgaaccctgc ggggcttagg tgctggtgcg
     4441 tccatttggc aatggacttg gccgtgtgcc ggacttcgtt tgcggggagc ggctgctcga
     4501 actgggcgtt atatcctgat gcccggtcaa cacaagcggc caaccattcg gggtaatccg
     4561 gccagccctg ccggatcgct ttataggccc acaggcgcgt tttctcgaac agggtacagt
     4621 ttcgcccaag cccgtactca ggaagcgatt tgcggccatt gtaggagctc agatcgaggt
     4681 aatcggatag ccaggctaaa tcgtagagcc ggggttccca tacctgcacc agccagtgct
     4741 catgcagggg gttcttgcag atcagcccgg agtagcccat atccgcccct aatttctccc
     4801 ggagcgcggc ctcgatggcg gcggcatacc gcagaggggc tgcatttcca tccggggcag
     4861 tgcggatcgg cgtatccagt ccgtagatca gatgcgcgtg gccgttggcc ttgttcgtga
     4921 cggtaatgtt aggggctgga gcgcctacgt catcccaaga taacgtagcg gctgcatggt
     4981 ctacgtcaaa aacgatccag tggcggtgcg ttggcccgtt cggctggatg taacgcgcac
     5041 caagggcgcg ggcaacatcc gcaatccgta ggcccgttgt aagctcgtcg gaatagtagg
     5101 gtttccgggg caggcgctcc cgaaacagct caagctgtgc gcactttctc aagcaccacc
     5161 gcgcttattg gcggcgtggg gttgtttggc atgatccatg tgatagactt ctcttgtgat
     5221 tcgtcacggc ccccgttttt cgcagctccg ccaagatgca agcgataacg ggggtttgtc
     5281 ttttctggac aatacgttac agcaggcaca tcctagagag caacgttagt tgcatgtaac
     5341 ctttcaccct ggaacatatt tccacctcct tctgcttctg cgctgttttg aattgcttga
     5401 tctacgcctc taactaggtc gatccttggg attgagtggc accattgtca gtgcaagaat
     5461 actgatccat ataagtctct ctagtcggac aacaccaagt gttgaagaga tcaaaaatca
     5521 acctatatcg gaggattcat gtctgacatt gacagatgcc cccatgaaag caaagcgaat
     5581 gcggtcccaa agcgcctctg tagaaacgaa tttgagcgag gcatttgatg ccatcgtctg
     5641 cgtcccaagc gccggcaagg acggcctggt tgacctatag taaaccggta gatgaaaatg
     5701 atggagaacc aatatcagcg ccttcttgga ctttttcgtc aaacgcttta gcagccaaga
     5761 aagcctacaa tccttgatgg gctaggcact ttatcgcagc ggcattcacc tttactgaca
     5821 tcgccactcc tttattcaca cccccatgtt gagtgaccat ggccattgag ataacaaaaa
     5881 agcctagcaa attggctagg ctttttacgt ttaggaatgt gatgctaatg ctcaggctga
     5941 aagttaacgc gcggagctta agtcgattag gcctgaccga ttaagtatga gccattaact
     6001 acaagcgcat cgagcatcag cattttgtcc atatatcttt acagcttata tgacggctat
     6061 ttcacacaaa tacgcgctaa ccacttctag ggaataattt tctcctgttt gagcacttct
     6121 tttgtgatgg cttggcgcag ccctaaacca tccgatgtgg gcatatccat attcatcgca
     6181 aaaaaccaca cattatcatc aagttcaacc caaccaaccc accagccaat cttaggttcg
     6241 attctagtcg agtatcccgt tttagcccga ataatatagt cgccattggc ttcggtcagc
     6301 atggcttgtt tcacgatgcg ctgactacgc tcagaaacgt gcagcttgtt gtgatacagc
     6361 ttgcgtaaaa aagcgatttg ctgggtagcc gaaatgcgaa taccaccatc gagccaaaaa
     6421 ctgtctacat tgcccgagat atcctcattg ccataatcga aggcgtgcag cattttactc
     6481 atacgtgcct caccaatttg gcgggcaaat tcttgataaa caggcacaac tgagtacttc
     6541 atcgcggtaa ttaagtcatg gtcacgattc caagcggcga tatcacgcgt ctgtccatcc
     6601 cacttaaaga cttggtgttc atccttaacc acgcccaaat cgagggcgat caagctattg
     6661 ggaattttaa aggtagatgc gggtaaaaat gcttggttcg cccgtttaag attattggta
     6721 aatccttgct gcttattctc attccagagc acaactacgc cctgtgattt atgttcagta
     6781 aagtgagcat tccaactttt gttttcttgc cattcctttg ctaccgctgg cattccgata
     6841 atcgatgcca ccaaaaacac agccgataag gctaatacac gcataacgtc cccttgctta
     6901 atgcaatata tccatacaaa ttaagtgcta agcttatcat tctctgccct ctcattccct
     6961 agcaaactgc tgaccaattg tggcaacaat caggcacaag ctttaactat tacctaaatt
     7021 ccacgtgtgt tttttattag cttcaaaaat cactatttca cgaagaattt agactgcttc
     7081 tcacacattg taacattatt tacaaccacc tttcaatcat ttttgataaa tcattgattt
     7141 catctttgct gcaatgatac ttaataaact ctgcaagtta tcggggagta tgggattttt
     7201 taacctatct aaagcgcttg aggaggtgtc tgatgctttt ggggctaaag aaaccgcaat
     7261 cgctagtgcc aagttgttag gtaagggagt tgttaaccta gctcattttg cagcaacaac
     7321 aggtttggat tcggtggtca aacaaacgag caacaaagtt ctcaacagcg agtcttcaac
     7381 cgaagaacaa cgagaacagg ctaaaaagtc tgccgcatgg gcagacgcga gaatcgccgc
     7441 tagaaataaa gcagaaaaca ccgaagacta gctgaacgca catttcgaca atgatgtgga
     7501 ctacatcgtt gatcataact tcacggtgcg ctagcttcca tagaagccaa ctaataacaa
     7561 ggagttagca tgtctgattc agatttcaaa aatagccctg agtatgatga ctggattgaa
     7621 tctggtggta gagatgaaga ttacagttac tactatgaaa aatggcatag aagaactcaa
     7681 cgataattcc cgccgataaa aggaaagtga tagcactttc ctttttttat aactgacggc
     7741 cgttttgttc cgtcaacgct caaagcctcg cgatctgtct ccacgctccc agcctcgatc
     7801 cagtgccttc aggagcctgt gttccctttc ttttgcaact tgctgactag cccggtcaag
     7861 ccctccagct gcttgctcaa gccgtccact tgctgcgtta agccgttcac ctgcctcgtc
     7921 aatcgctctg ctgctgccct gctgatctcg gcctcttgtt tggtgctctc gtacatgctc
     7981 gccaagttcg cgtagctttc ctgccatgcc ttgtgctgct gctcgtatgc gctctgcaaa
     8041 gcctcgaacg cgcagagcaa ttcccgctcc aatcccgtca tttagtaccc cttcggcacg
     8101 atccaatagg tatccttccc ccaaacggtt ggattggggt cggagatctg cacgcaaagg
     8161 tgctccaatt tcttcccgca ctgggtcagc tttagcttgc cgcccttctc tgccaaggct
     8221 tggagagtgg cttgccgctc cgctatcaat tccccctgat accagaggat cccgctcgtt
     8281 gcgctcaaga acaggacgat gctgaccagc atccagatcc acgttttgag caacagccag
     8341 cccattttct ggctgtgctc ggcaatagcg gcgctcattt tctcctcgct tgatttcaac
     8401 gccccggaga tagctttctc gtgcctcgtg aattcttcgg tcagacgctt ctcgatagcg
     8461 ttggcctgct gcttcgagct ctgctcgaag tccttcgcca tgtctaaaat cttgctcata
     8521 cagcaccccc tttaatcgga tgttccgccc cccgtccggg tcggcaatgc tcaggctgtt
     8581 tttggtttcg cgtaccacgc tgaaacccgc accctgcaag gctttgcgca catcatcgcg
     8641 gctctggatc tcccctgctc cggccatgcg taaaagcccg tctgtaatgg cctgagcggc
     8701 ctccttgtgc cccctcggca aatcctttgg cgtgataacg gcctgacggt ttaacggatc
     8761 gtccgggtcg tgcaatttca gctccccgtt taccacggtt cgccacgcat cgaggcgcgg
     8821 attgtcggcc ttgtggaagt agggttgcag ccgcttcccg ctctgcaatt cgatgttcgg
     8881 gactacaaaa ttcagctcca gccgcccctt gtcgcggtgc tctacccaca aaacagcgta
     8941 ctggttgccc tccagtccgg gcaggagcgc cttttcaaag tcgctcatca gcttgtcctt
     9001 ggtcgccctg ggtaaatcag cctcggcaaa cgacagaacg ccagaggtgt attttttggc
     9061 gtagggactg gaatcaatca gggcttcaat caccgcaggg ttgccccggt cgagcgttgc
     9121 gccttcccgg ttcccgtcac ggcccaacaa gtagccaaca ggcccggagc cccggcctac
     9181 cccacggtta tggaatttga tgatcatggt gcagttcctc cagctcgcgg cctatagcgg
     9241 acagctccgc tatgatgcgc agccgatcca acggccccca ctccccggaa ttaacgcgcc
     9301 gggcgatctg gttcaggttg ttgcccattc cggccaactg ccgcaagagc gccggatcta
     9361 cggtaggcgc cggcgttcgg cgctggggtt gcgcaacacc gagacattgc tcccgcatcc
     9421 actccgccag ttgggccttg gggcagagct cccgcaggcg ctcaagctcc gcatctgaga
     9481 cccggatctt gatagagcgg aggcgcttta acggctggcc agccatgcag aggcctccgc
     9541 taaggcgtcc tcatagaggc tatcgagtag cgtctggagc gccttttcat cccgaaaatc
     9601 gcagtgctcc ggcaagggga aaacaccccc gccgacctct tcgccgttct ccagcaaacg
     9661 cagattccag cccccgccaa gaccgagagt gcggggggtg atttcgtagt cgtattccgt
     9721 tgttgtgggt tcgtcactta ccatagctaa cacctcatcg gtggaaattt ttccattgat
     9781 ataatcttca agtagaattt tgaattcatc ggttatggcc tccccctcta gttggaaact
     9841 agaaataacg ccattgactg ccttccttct cgaatctttc tctatctgtg ttgttgacat
     9901 agggcctcgt ccattctcca gagccaagaa gccaccgcgc agaccgtccc gaaggggcgg
     9961 atcgagcggg ggccacaggg ggtttcgggg gggcgtagca cccctgaaca aggccacaga
    10021 ggcccgagac cgcgaagcag gtagaggggc ctctgtgtag ccttgcttag tacattattc
    10081 aaccatgggc gggatactgc aagccctaga gcaagatcaa gggaaagagc ggctccgtgg
    10141 ataactgctg ccgcccacac cccccgaaaa aacagggggg cgatggacgg acaggcgcag
    10201 ttgtccacct cgccaaagat ccgcgctaaa aggatttttt aaggctaaaa gacaagagcg
    10261 caggaacccc cctaaagggg gctattatct gataaggctt gtttcgatca cggacgatca
    10321 cgctttaggt aaacgctcac cgtactgggg gagatttgaa gatcgtccgc tatatcgcgg
    10381 ttggtgtacc cctgagcctt aagccgcaga acctccggca atagatcgga cttgtcctta
    10441 ccgctattgc tcggccttcc ccctcccttg gataccttgc cgcccttcgc acctctccgg
    10501 gcctgctcct ctctgaaccc tgcggggctt aggtgctggt gcgtccattt ggcaatggac
    10561 ttggccgtgt gccggacttc gtttgcgggg agcggctgct cgaactgggc gttatatcct
    10621 gatgcccggt caacacaagc ggccaaccat tcggggtaat ccggccagcc ctgccggatc
    10681 gctttatagg cccacaggcg cgttttctcg aacagggtac agtttcgccc aagcccgtac
    10741 tcaggaagcg atttgcggcc attgtaggag ctcagatcga ggtaatcgga tagccaggct
    10801 aaatcgtaga gccggggttc ccatacctgc accagccagt gctcatgcag ggggttcttg
    10861 cagatcagcc cggagtagcc catatccgcc cctaatttct cccggagcgc ggcctcgatg
    10921 gcggcggcat accgcagagg ggctgcattt ccatccgggg cagtgcggat cggcgtatcc
    10981 agtccgtaga tcagatgcgc gtggccgttg gccttgttcg tgacggtaat gttaggggct
    11041 ggagcgccta cgtcatccca agataacgta gcggctgcat ggtctacgtc aaaaacgatc
    11101 cagtggcggt gcgttggccc gttcggctgg atgtaacgcg caccaagggc gcgggcaaca
    11161 tccgcaatcc gtaggcccgt tgtaagctcg tcggaatagt agggtttccg gggcaggcgc
    11221 tcccgaaaca gctcaagctg tgcgcacttt ctcaagcacc accgcgctta ttggcggcgt
    11281 ggggttgttt ggcatgatcc atgtgataga cttctcttgt gattcgtcac ggcccccgtt
    11341 tttcgcagct ccgccaagat gcaagcgata acgggggttt gtcttttctg gacaatacgt
    11401 tacagcaggc acatcctaga gagcaacgtt agttgcatgt aacctttcac cctggaacat
    11461 atttccacct ccttctgctt ctgcgctgtt ttgaattgct tgatctacgc ctctaactag
    11521 gtcgatcctt gggattgagt ggcaccattg tcagtgcaag aatactgatc catataagtc
    11581 tctctagtcg gacaacacca agtgttgaag agatcaaaaa tcaacctata tcggaggatt
    11641 catgtctgac attgacagat gcccccatga aagcaaagcg aatgcggtcc caaagcgcct
    11701 ctgtagaaac gaatttgagc gaggcatttg atgccatcgt ctgcgtccca agcgccggca
    11761 aggacggcct ggttgaccta tagtaaaccg gtagatgaaa atgatggaga accaatatca
    11821 gcgccttctt ggactttttc gtcaaacgct ttagcagcca agaaagccta caatccttga
    11881 tgggctaggc actttatcgc agcggcattc acctttactg acatcgccac tcctttattc
    11941 acacccccat gttgagtgac catggccatt gagataacaa aaaagcctag caaattggct
    12001 aggcttttta cgtttaggaa tgtgatgcta atgctcaggc tgaaagttaa cgcgcggagc
    12061 ttaagtcgat taggcctgac cgattaagta tgagccatta actacaagcg catcgagcat
    12121 cagcattttg tccatatatc tttacagctt atatgacggc tatttcacac aaatacgcgc
    12181 taaccacttc tagggaataa ttttct
//
