LOCUS       NZ_CP137405             8334 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP11_L3_11.22_ST147 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP137405
VERSION     NZ_CP137405.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951960
            Assembly: GCF_033254545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 204x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:39:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,592
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,299
            CDSs (with protein)               :: 5,299
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 76 of 168
            Pseudo Genes (incomplete)         :: 100 of 168
            Pseudo Genes (internal stop)      :: 25 of 168
            Pseudo Genes (multiple problems)  :: 26 of 168
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137405.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP11_L3_11.22_ST147"
                     /isolation_source="neonatal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-03"
                     /genotype="ST147"
     gene            complement(220..852)
                     /locus_tag="R5O48_RS27905"
                     /old_locus_tag="R5O48_27905"
     CDS             complement(220..852)
                     /locus_tag="R5O48_RS27905"
                     /old_locus_tag="R5O48_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(852..1967)
                     /locus_tag="R5O48_RS27910"
                     /old_locus_tag="R5O48_27910"
     CDS             complement(852..1967)
                     /locus_tag="R5O48_RS27910"
                     /old_locus_tag="R5O48_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2312..2425)
                     /locus_tag="R5O48_RS27915"
                     /old_locus_tag="R5O48_27915"
                     /pseudo=""
     CDS             complement(2312..2425)
                     /locus_tag="R5O48_RS27915"
                     /old_locus_tag="R5O48_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2841..3032
                     /locus_tag="R5O48_RS27920"
                     /old_locus_tag="R5O48_27920"
     CDS             2841..3032
                     /locus_tag="R5O48_RS27920"
                     /old_locus_tag="R5O48_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3032..3433
                     /locus_tag="R5O48_RS27925"
                     /old_locus_tag="R5O48_27925"
     CDS             3032..3433
                     /locus_tag="R5O48_RS27925"
                     /old_locus_tag="R5O48_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3779..4021)
                     /locus_tag="R5O48_RS27930"
                     /old_locus_tag="R5O48_27930"
     CDS             complement(3779..4021)
                     /locus_tag="R5O48_RS27930"
                     /old_locus_tag="R5O48_27930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(4387..5019)
                     /locus_tag="R5O48_RS27935"
                     /old_locus_tag="R5O48_27935"
     CDS             complement(4387..5019)
                     /locus_tag="R5O48_RS27935"
                     /old_locus_tag="R5O48_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(5019..6134)
                     /locus_tag="R5O48_RS27940"
                     /old_locus_tag="R5O48_27940"
     CDS             complement(5019..6134)
                     /locus_tag="R5O48_RS27940"
                     /old_locus_tag="R5O48_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(6479..6592)
                     /locus_tag="R5O48_RS27945"
                     /old_locus_tag="R5O48_27945"
                     /pseudo=""
     CDS             complement(6479..6592)
                     /locus_tag="R5O48_RS27945"
                     /old_locus_tag="R5O48_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            7008..7199
                     /locus_tag="R5O48_RS27950"
                     /old_locus_tag="R5O48_27950"
     CDS             7008..7199
                     /locus_tag="R5O48_RS27950"
                     /old_locus_tag="R5O48_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7199..7600
                     /locus_tag="R5O48_RS27955"
                     /old_locus_tag="R5O48_27955"
     CDS             7199..7600
                     /locus_tag="R5O48_RS27955"
                     /old_locus_tag="R5O48_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7946..8188)
                     /locus_tag="R5O48_RS27960"
                     /old_locus_tag="R5O48_27960"
     CDS             complement(7946..8188)
                     /locus_tag="R5O48_RS27960"
                     /old_locus_tag="R5O48_27960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(2313..2720)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          complement(6480..6887)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 aattgtttta agtgaataga gagaatataa cttaagggag catcttgtgc tgggtctttt
       61 acattagatc tgcacaatca ttgttttggc atctggtgat gtctttagga cgtttgagat
      121 tgctgataac taaagtgtat gatgctttct atacgttatc cggcgtttaa taacaccctg
      181 gtgataagcc agcccgtgaa gggctggctt gatttggatt tatatttttg aaataaaatc
      241 ttttatttca tcaaagcaat tgtctgctgt gtttattatt ccatttttca tggatattcc
      301 tattcttgag tcggattctc tattgcagct aagatagact tgtaccttag cttttttttc
      361 gtttgttgga taagtgtact ctttgtcact cgctgagatg tcatctttat tatttttaat
      421 tttttcgaaa aattctatta tgtattcata taacgctcga gtgcttgcgt gttcaattat
      481 cagatcttca atggcacctt gcttgtcttt tcctggcatt ataaagaaag aaatttttgt
      541 attgccattc atagctatct cgttatgcga gctgggggag gagatttgta gttctcgcaa
      601 tgtggcaatt attgatcttt ctgtgtcaga aaatgatgtg tcagcatcta atataataaa
      661 aatactttct ttttcatcga aagaatcaat gtttttgaat gctctaagtg cttgttttaa
      721 ttttgatttt ccatttattt tttcgacaaa aacatcggta atgccttgta tttcaagtag
      781 gttgcataga aaatgaaaat catcatcacc ctcaacgaaa atgatctttt tgctattatt
      841 tatgtctttc attatcttac ctcccagtcg ttctcaactg agtaggagaa atcatccata
      901 ttgaattgta cggctgttgg ttttagggag ttcttttctc ttcctagtct aatataagcg
      961 atatcttcat ttttgctatc tatttcgttt attgctcgta tcacatcttg actatgagtt
     1021 gttgcaaata tttgattgtt gttttgtttt gcagatgaaa ttaatgattt tattatgtct
     1081 ttaattactg agtaatgtat tccgttctcg atttcatcaa taagaataat tgagttttcc
     1141 tgtaccagca ctgagctgat aaaagataat gctcgactga taccctcgcc aagcattgat
     1201 atttcattta gctcactaaa tccaatgtct aaatatattt ccttattgtt acctatcgca
     1261 gatattgcta tatctacaat tctgtcatct atttttttta agttttctag tatgtctttt
     1321 ttcctttttt gtgttagtag gctagataca tttgtaattg ttgcctcttt gtttatcttt
     1381 ctggatgttg ttattattgt tgctgtctta aatttataat taatcccatc cctttcgttt
     1441 tttatttcgg attttatttc tacgccatct tctttcaatt gcatagagaa aagcttattg
     1501 gaaaccctgt ttttcgcatt gggtgtctcg gtcacattta tttttaaaga gtttactgtg
     1561 gcctgagaac cgatagtttg acgaggtatg ttttggttag atagtacggt gtcaacgttt
     1621 agagacaaac ttgattctaa tctctgattt gtttctgttt catatgtttg tgttacttca
     1681 gagcgttcat ctcgaatgac tatggacatc ttttgtgatg tgtccatatc atgaaagtaa
     1741 ctaacccaaa atggcttgtt tttgtttatt tctgccattt cttttctgaa tgcttgaatg
     1801 tttaacaaaa cagccgggtt ttttacatca taaaaacaaa aaatagcatc cagtatagat
     1861 gttttcccgt agttgttttt cccagctatg atatttaaag tctttaattg ctttagatat
     1921 agctcattga agcatttgaa gttctttaaa cttatttcct ttatcatgat agcctctgtt
     1981 cgtgtttttg ttatatgcca tacgatttgt tagtacctta ataaaatgta cagctttcat
     2041 tgtgattagc tagactttat gtcaaagatt aactttgatt gcttgtgttt taatcgattt
     2101 tttttacttt cttcttgaga ttatctggca gcagccgtaa gcttccggct gtgaacgaaa
     2161 aaaaccgcct tggcgggcgg ttttttcgaa aggttcagta gagttggcgc tctatgaacc
     2221 gaggtaactg gcttaaaggg agcgcagtca ccaaaactgt tcgtttagtt tagctttaac
     2281 tggcgtataa cttcaagact aactccctaa atcagttacc agtggctgct gccagtggcg
     2341 ctttgtcgtg tctttccggg ttggactcaa gacgatagtt accggattag gcgcagcagt
     2401 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac
     2461 tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac
     2521 cgaaaggcag gaacaggaga gcgcacgagg gagccatcag ggggaaacgc ctggtatctt
     2581 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattccgtg atgctcgtca
     2641 ggggggcgga gcctatggaa aaacggccgc tgtgcggcct ttccttcttt cctccatgcg
     2701 attactcggt tttctcgagc gtttctacgc atgctcctgc cgctcgccgc agccgaacgg
     2761 caggagcgta gcgactgagt gagcgaggaa gcggaaaaga ggagatccct acactgacca
     2821 ctgacgctct gaggcctctt atgacgaaac aacagaaaac agcgctgaac atggctaaat
     2881 tcatccagaa tcagtctctg ctcctgctgg agaagcttaa cgagcttgat ctcgatgtcg
     2941 aggccgacct ttgtgagaaa ctccacgatg atgcggagca tcttttccgt acactgtcct
     3001 cgagactgga tgcattacag gatggtaact gatggccaga gtgtctatta gtgaggctgc
     3061 ccgactgacg ggtaagagta gaacaacact gcacaggtta attaaagcag gtgacctgtc
     3121 cacctgtagc ggtgagcgca atgcaaaaat ggtagatact tcagagcttt tacgtgtctt
     3181 tggccccttt gagcaaccga agggtgaaca ccattctgga caggtctctg gacagcgtga
     3241 tacagcctct gctgaccaga gtgaacaggt gatccgtcat ctgaaacagg agattgaaca
     3301 cctgaacacc ttacttttgt ccaaggattc gcacattgac agcctgaaac aggccatgct
     3361 ccttcttgaa aatcaccagg caaaattaac tgaatccccg gcgccatggt ggaaattctg
     3421 gaaaaaatcc tgaccccgtc atgcttcagt tttcctctcc ggccatccca gggagttcct
     3481 gaaccttccc aaaaatcgtc agatctctga acgctcctca ccactaaaaa tacccacacc
     3541 ccacgcaaaa ttaaaatttt gcagattcct tatttacaac aatgggttaa tgaaaaataa
     3601 atgtgcggtg atgtgttaca aagtgcagat ccaagtcgtg gacgactcta tggcgaatcg
     3661 ctgacgaatt gcccacgatt catatgtgaa tcacgcttta ttatcatatt gttatgatga
     3721 atcgactctt gattcaattc atcactttgg gtgttttttg ctcatggtga ctcttgattc
     3781 aattcacgat tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac
     3841 gtgattgccg gccgtcgacg aacccgattc ctgaaggtgc tgaaaaccgg ttttccggct
     3901 gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggagg
     3961 agaagaacgc cggaaacagg acttcccctg gctgaatggt gtgaatttcc gcgctatgca
     4021 cttgcgcgca tactcatgca tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc
     4081 gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa cgtcctgagt ccgttttagc
     4141 ggtgcgtaca attatgattt tatgttaaat tgttttaagt gaatagagag aatataactt
     4201 aagggagcat cttgtgctgg gtcttttaca ttagatctgc acaatcattg ttttggcatc
     4261 tggtgatgtc tttaggacgt ttgagattgc tgataactaa agtgtatgat gctttctata
     4321 cgttatccgg cgtttaataa caccctggtg ataagccagc ccgtgaaggg ctggcttgat
     4381 ttggatttat atttttgaaa taaaatcttt tatttcatca aagcaattgt ctgctgtgtt
     4441 tattattcca tttttcatgg atattcctat tcttgagtcg gattctctat tgcagctaag
     4501 atagacttgt accttagctt ttttttcgtt tgttggataa gtgtactctt tgtcactcgc
     4561 tgagatgtca tctttattat ttttaatttt ttcgaaaaat tctattatgt attcatataa
     4621 cgctcgagtg cttgcgtgtt caattatcag atcttcaatg gcaccttgct tgtcttttcc
     4681 tggcattata aagaaagaaa tttttgtatt gccattcata gctatctcgt tatgcgagct
     4741 gggggaggag atttgtagtt ctcgcaatgt ggcaattatt gatctttctg tgtcagaaaa
     4801 tgatgtgtca gcatctaata taataaaaat actttctttt tcatcgaaag aatcaatgtt
     4861 tttgaatgct ctaagtgctt gttttaattt tgattttcca tttatttttt cgacaaaaac
     4921 atcggtaatg ccttgtattt caagtaggtt gcatagaaaa tgaaaatcat catcaccctc
     4981 aacgaaaatg atctttttgc tattatttat gtctttcatt atcttacctc ccagtcgttc
     5041 tcaactgagt aggagaaatc atccatattg aattgtacgg ctgttggttt tagggagttc
     5101 ttttctcttc ctagtctaat ataagcgata tcttcatttt tgctatctat ttcgtttatt
     5161 gctcgtatca catcttgact atgagttgtt gcaaatattt gattgttgtt ttgttttgca
     5221 gatgaaatta atgattttat tatgtcttta attactgagt aatgtattcc gttctcgatt
     5281 tcatcaataa gaataattga gttttcctgt accagcactg agctgataaa agataatgct
     5341 cgactgatac cctcgccaag cattgatatt tcatttagct cactaaatcc aatgtctaaa
     5401 tatatttcct tattgttacc tatcgcagat attgctatat ctacaattct gtcatctatt
     5461 ttttttaagt tttctagtat gtcttttttc cttttttgtg ttagtaggct agatacattt
     5521 gtaattgttg cctctttgtt tatctttctg gatgttgtta ttattgttgc tgtcttaaat
     5581 ttataattaa tcccatccct ttcgtttttt atttcggatt ttatttctac gccatcttct
     5641 ttcaattgca tagagaaaag cttattggaa accctgtttt tcgcattggg tgtctcggtc
     5701 acatttattt ttaaagagtt tactgtggcc tgagaaccga tagtttgacg aggtatgttt
     5761 tggttagata gtacggtgtc aacgtttaga gacaaacttg attctaatct ctgatttgtt
     5821 tctgtttcat atgtttgtgt tacttcagag cgttcatctc gaatgactat ggacatcttt
     5881 tgtgatgtgt ccatatcatg aaagtaacta acccaaaatg gcttgttttt gtttatttct
     5941 gccatttctt ttctgaatgc ttgaatgttt aacaaaacag ccgggttttt tacatcataa
     6001 aaacaaaaaa tagcatccag tatagatgtt ttcccgtagt tgtttttccc agctatgata
     6061 tttaaagtct ttaattgctt tagatatagc tcattgaagc atttgaagtt ctttaaactt
     6121 atttccttta tcatgatagc ctctgttcgt gtttttgtta tatgccatac gatttgttag
     6181 taccttaata aaatgtacag ctttcattgt gattagctag actttatgtc aaagattaac
     6241 tttgattgct tgtgttttaa tcgatttttt ttactttctt cttgagatta tctggcagca
     6301 gccgtaagct tccggctgtg aacgaaaaaa accgccttgg cgggcggttt tttcgaaagg
     6361 ttcagtagag ttggcgctct atgaaccgag gtaactggct taaagggagc gcagtcacca
     6421 aaactgttcg tttagtttag ctttaactgg cgtataactt caagactaac tccctaaatc
     6481 agttaccagt ggctgctgcc agtggcgctt tgtcgtgtct ttccgggttg gactcaagac
     6541 gatagttacc ggattaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca
     6601 gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc
     6661 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
     6721 ccatcagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt
     6781 gagcgtcaga ttccgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cggccgctgt
     6841 gcggcctttc cttctttcct ccatgcgatt actcggtttt ctcgagcgtt tctacgcatg
     6901 ctcctgccgc tcgccgcagc cgaacggcag gagcgtagcg actgagtgag cgaggaagcg
     6961 gaaaagagga gatccctaca ctgaccactg acgctctgag gcctcttatg acgaaacaac
     7021 agaaaacagc gctgaacatg gctaaattca tccagaatca gtctctgctc ctgctggaga
     7081 agcttaacga gcttgatctc gatgtcgagg ccgacctttg tgagaaactc cacgatgatg
     7141 cggagcatct tttccgtaca ctgtcctcga gactggatgc attacaggat ggtaactgat
     7201 ggccagagtg tctattagtg aggctgcccg actgacgggt aagagtagaa caacactgca
     7261 caggttaatt aaagcaggtg acctgtccac ctgtagcggt gagcgcaatg caaaaatggt
     7321 agatacttca gagcttttac gtgtctttgg cccctttgag caaccgaagg gtgaacacca
     7381 ttctggacag gtctctggac agcgtgatac agcctctgct gaccagagtg aacaggtgat
     7441 ccgtcatctg aaacaggaga ttgaacacct gaacacctta cttttgtcca aggattcgca
     7501 cattgacagc ctgaaacagg ccatgctcct tcttgaaaat caccaggcaa aattaactga
     7561 atccccggcg ccatggtgga aattctggaa aaaatcctga ccccgtcatg cttcagtttt
     7621 cctctccggc catcccaggg agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg
     7681 ctcctcacca ctaaaaatac ccacacccca cgcaaaatta aaattttgca gattccttat
     7741 ttacaacaat gggttaatga aaaataaatg tgcggtgatg tgttacaaag tgcagatcca
     7801 agtcgtggac gactctatgg cgaatcgctg acgaattgcc cacgattcat atgtgaatca
     7861 cgctttatta tcatattgtt atgatgaatc gactcttgat tcaattcatc actttgggtg
     7921 ttttttgctc atggtgactc ttgattcaat tcacgattcg aatcgccggt gaatcgtgtg
     7981 gtgagtcgtc cggcgacttg ccataacgtg attgccggcc gtcgacgaac ccgattcctg
     8041 aaggtgctga aaaccggttt tccggctgcg ccggcaacgc ttcacggcca cgcaataaat
     8101 tgcgtccccg ttcaccgttt tcggaggaga agaacgccgg aaacaggact tcccctggct
     8161 gaatggtgtg aatttccgcg ctatgcactt gcgcgcatac tcatgcatgc cgtaaaaaca
     8221 gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctgttttac cggtttcccg
     8281 tcagaaacgt cctgagtccg ttttagcggt gcgtacaatt atgattttat gtta
//
