LOCUS       NZ_CP137402             8104 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP119_WM3_21.23_ST147_NDM1 plasmid
            unnamed5, complete sequence.
ACCESSION   NZ_CP137402
VERSION     NZ_CP137402.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951961
            Assembly: GCF_033253665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 354x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:59:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,867
            CDSs (total)                      :: 5,742
            Genes (coding)                    :: 5,589
            CDSs (with protein)               :: 5,589
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 71 of 153
            Pseudo Genes (incomplete)         :: 88 of 153
            Pseudo Genes (internal stop)      :: 29 of 153
            Pseudo Genes (multiple problems)  :: 28 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137402.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP119_WM3_21.23_ST147_NDM1"
                     /isolation_source="pooled swabs"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-05"
                     /genotype="ST147"
     gene            join(7877..8104,1..93)
                     /locus_tag="R5O49_RS29290"
                     /old_locus_tag="R5O49_29280"
     CDS             join(7877..8104,1..93)
                     /locus_tag="R5O49_RS29290"
                     /old_locus_tag="R5O49_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_055318048.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPARACRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            302..>733
                     /locus_tag="R5O49_RS29295"
                     /old_locus_tag="R5O49_29285"
                     /pseudo=""
     CDS             302..>733
                     /locus_tag="R5O49_RS29295"
                     /old_locus_tag="R5O49_29285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            771..1256
                     /locus_tag="R5O49_RS29300"
                     /old_locus_tag="R5O49_29290"
     CDS             771..1256
                     /locus_tag="R5O49_RS29300"
                     /old_locus_tag="R5O49_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1260..1472
                     /locus_tag="R5O49_RS29305"
                     /old_locus_tag="R5O49_29295"
     CDS             1260..1472
                     /locus_tag="R5O49_RS29305"
                     /old_locus_tag="R5O49_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1980..2438)
                     /locus_tag="R5O49_RS29310"
                     /old_locus_tag="R5O49_29300"
     CDS             complement(1980..2438)
                     /locus_tag="R5O49_RS29310"
                     /old_locus_tag="R5O49_29300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            3804..4145
                     /locus_tag="R5O49_RS29315"
                     /old_locus_tag="R5O49_29305"
     CDS             3804..4145
                     /locus_tag="R5O49_RS29315"
                     /old_locus_tag="R5O49_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4354..>4785
                     /locus_tag="R5O49_RS29320"
                     /old_locus_tag="R5O49_29310"
                     /pseudo=""
     CDS             4354..>4785
                     /locus_tag="R5O49_RS29320"
                     /old_locus_tag="R5O49_29310"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4823..5308
                     /locus_tag="R5O49_RS29325"
                     /old_locus_tag="R5O49_29315"
     CDS             4823..5308
                     /locus_tag="R5O49_RS29325"
                     /old_locus_tag="R5O49_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            5312..5524
                     /locus_tag="R5O49_RS29330"
                     /old_locus_tag="R5O49_29320"
     CDS             5312..5524
                     /locus_tag="R5O49_RS29330"
                     /old_locus_tag="R5O49_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6032..6490)
                     /locus_tag="R5O49_RS29335"
                     /old_locus_tag="R5O49_29325"
     CDS             complement(6032..6490)
                     /locus_tag="R5O49_RS29335"
                     /old_locus_tag="R5O49_29325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          3538..3717
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          7590..7769
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(3075..3555)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          complement(7127..7607)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          84..1588
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4136..5640
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7878..8104
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.312"
                     /coverage="100.0"
                     /evalue="2.95e-38"
ORIGIN
        1 gtgcagattg tggccgccct gatggcaatc gacgcgggac tggagcggct gcggcatgcg
       61 gtgctggaaa aaggggggga tgatgatcgt taaatttcat ccccgggggc gcggtggtgg
      121 tgccggtccg gtggattacc tgctgggaaa agaccggcag cgcgaggggg cgaccgtgct
      181 gcagggaaaa ccggaggaag tccgggagct tatcgatgcc tcgccgtatg cgaaaaagta
      241 cacctccggc gtgctgtcgt tcgcggaggc tgaactgctg cccgggcagc gggagcagat
      301 catggccagc tttgagcggg ttctgatgcc cgggctcgat aaagaccagt acagcattct
      361 gtgggtggag cacaccgaca aggggcggct ggagctgaat ttcctgatcc ccaacactga
      421 actgctgacc gggaaacgcc tgcagccgta ttacgatcgc gccgaccggc cgcgcatcga
      481 tgcctggcag accgtggtga acggcagact gggactgcat gatccaaacg ccccggagaa
      541 ccggcggtta ctggttacgc cgtccgcact gccggaaacg aagctggaag ccgctcaggc
      601 gattacacgt gggttgctgg ccttagcctc atccggggag ctcaaaactc gcgaggacgt
      661 cacaggtgcg ctcacagcgg caggttttga ggtggtccgc actacaaaaa acagtatcag
      721 cattgccgac ccggacgggg ggcgaaacat ccgactgaag ggagccattt atgagcagtc
      781 ttttagcgct ggcgaaggac ttggagcagc aatcgaaagc gcagcagcag agtaccggcg
      841 agatgctgaa agccgcattc agcgagcacg agaaatctgt aaaagcggaa ctgagcgcaa
      901 gcgcgaagag aatcagcgac gccatcagcg cccacgagca gggtatgacc gcggcgatgc
      961 agtcgaaccg cctgagcgtg ctgcgtatgg tcggccggac gtggctgacc atcaccctgg
     1021 tctcggtact gctgatcgcc atgagcggga gcattctgtg gtggcagggg cagcagatca
     1081 ccgggaatta ccagactatc cgggcgcagg agcggacgca ggccatgttg tcggagaaaa
     1141 accacggcgt tcagctgtcg ctctgcgggg agaagaaact gtcctgcgtc aaagtgaacc
     1201 cgaaagcggg agcttacggg gaggaaggga actggatggt actggagagg aaatagccca
     1261 tgacggaact ggaaaaacag ttgctgaccg cattagagca gctacatcag gactactcgc
     1321 aaaggctgga cgagtgggag agcgccttcg cggaatggca gaagatgtct ggtcttatgc
     1381 aacgggagaa cgcggcgctg aacgagcgcg tcacgcgctt gagtcagcag gtggcgaact
     1441 tgagcaggca gttacagcgt ttgagccagt agtgcagcgg catgaaatgg cggtggccgc
     1501 cgaacgggca tacgagcagc gacagcacga aaaagagctg gcggaagccc gttcccgtca
     1561 gcggagctat aacggtccga cgctgggctg acaggcttca ccagtgacaa accccacgaa
     1621 gaagggctca taaatttccc gctaaatacg gtcgtgatga aaacatcacg gcgtatacaa
     1681 cggagggatt atgagactgt ttaacgtgat tgagacggtg ctgaactgcc tgtacatggc
     1741 gctggagatc tgggactggc tggagaagca ttttcctgtt tttaaggcgt tcatccagat
     1801 tctggcctga aatcagaccg tatgcatctg gctgcatgac catgcagccg ggtaaaattt
     1861 atctgtcacg aacggggcgg tgtatcgggt ggtttgttgc cggttttacc tgactgccgc
     1921 cccgtttgtg acgaacatgt ccggagcggt gcgggcaaga gatgttatgg taaatcatat
     1981 tagttctgaa tgattcgggc tccaccagtc attactattt gagcaccacc tgacattttt
     2041 atacttgtac cctggctagt cattgtcgta tcaccttgta tgatcgtttt tccagtagca
     2101 tgttgacctc caccagtgac taaaattgac gcttgtactt gagatccatt tatcaaaaca
     2161 gttccggctt ggtgatcaaa ttttattccg ctcaatgctt ttaattcagc tccatctagc
     2221 tgaatttcac cattggctgc tgcagcctga atggccccct gcatctgaga aaataattta
     2281 tcaacttcgt caaaaggtaa tggtgtttca agcagtgagg cttgttgttc aacttcagga
     2341 tgtttacttg ctaacttccc tatgatgcct ttgattgcat cagaagtaag attgcttaat
     2401 gcgccagtga gaacggcaag tagtgtggtt tcaatcatta tatttccctc gttagattgc
     2461 tgtgatgcgt attatttact ctttataatc tccgattctg tccatatggt tagttgcact
     2521 attacgttta taagtgccac ttttaccata tatatatcca acgctacatt gtggacattt
     2581 tggcgtctcg tcattatgag tgaaatttat ttctcctccg acagataggg tttttacgtt
     2641 ttccggataa tctaatgtag ccacgccggt ccattcacaa ttcgagcaga caacatcgaa
     2701 agatgttggg atactcttgc agttgatgtt gatttgcata tgcataacct cgattttgca
     2761 cagaaattac agtgttatta gttgcatacc gactataacc tgagtttgtg atggagtggt
     2821 gtttttttgc tttgacttga gtaagagaaa gagatgatct tcttgagata atctctttct
     2881 gagcgtagtc ttttgctctg taaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt
     2941 tagttaatcc tggcagattt actaaccgtg gtaacagtct tgtgcgagac atgtcaccaa
     3001 agctgtcctt ccagtgtagc cgcagtaagg ccaccacttc aagaactctg aatacatctc
     3061 tcgcacatcc tgtttaccag tggctactgc caatggcgat ttgtcgtgcc ttttcgggtt
     3121 ggactcaaga cgatagttac cgaaaagagc gtggtagtcg gactgaacgg ggggatcgtg
     3181 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     3241 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aatggcagga acaggagagc
     3301 gcacgaggga gctaccaggg ggaaacgcct gggatcttta tagtcctgtc gggtttcgcc
     3361 accactgatt tgagcgtcag attttatgat actcgtcagg ggggcggagc ctatggaaaa
     3421 acggcaacgc cgcatcctct tcccggcttc tcctgatttg agcgctgctt gcgtatcccg
     3481 tcatctgtga gctggtaacg cccgccgcag tctcacggca ggagcgcagc gactgagtga
     3541 gcgaggaggc gtcatttcac ctttaccagc atgttgcact tacgcccgtc agccacattt
     3601 tccccttccc acatgaagca ctttcataca ttaccactcc gccgtaacat atttagccag
     3661 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac ccgctaaaag
     3721 ccactgacgc gcctgcggct tgtccaacac cgtgccgacc gggaaacatt tcccgcccgt
     3781 cccggtgtta tcaggaggct actctggcag acaaacgcaa caagatgctc acgatgtggg
     3841 tgacggaaga cgaacaccgg cggctgctcg agcggtgcga cggcaggcag ctggcggcgt
     3901 ggatgcgtca gtcctgcctc gatgagaagc cggcgcgtgc ctgcagactg ccgtcaatat
     3961 cgccggcgct gctgcgccag cttgccggta tgggtaacaa cctgaaccag atcgcccgga
     4021 aagtgaatgc cgttggtgcc ggtcatgatc gggtgcagat tgtggccgcc ctgatggcaa
     4081 tcgacgcggg actggagcgg ctgcggcatg cggtgctgga aaaagggggg gatgatgatc
     4141 gttaaatttc atccccgggg gcgcggtggt ggtgccggtc cggtggatta cctgctggga
     4201 aaagaccggc agcgcgaggg ggcgaccgtg ctgcagggaa aaccggagga agtccgggag
     4261 cttatcgatg cctcgccgta tgcgaaaaag tacacctccg gcgtgctgtc gttcgcggag
     4321 gctgaactgc tgcccgggca gcgggagcag atcatggcca gctttgagcg ggttctgatg
     4381 cccgggctcg ataaagacca gtacagcatt ctgtgggtgg agcacaccga caaggggcgg
     4441 ctggagctga atttcctgat ccccaacact gaactgctga ccgggaaacg cctgcagccg
     4501 tattacgatc gcgccgaccg gccgcgcatc gatgcctggc agaccgtggt gaacggcaga
     4561 ctgggactgc atgatccaaa cgccccggag aaccggcggt tactggttac gccgtccgca
     4621 ctgccggaaa cgaagctgga agccgctcag gcgattacac gtgggttgct ggccttagcc
     4681 tcatccgggg agctcaaaac tcgcgaggac gtcacaggtg cgctcacagc ggcaggtttt
     4741 gaggtggtcc gcactacaaa aaacagtatc agcattgccg acccggacgg ggggcgaaac
     4801 atccgactga agggagccat ttatgagcag tcttttagcg ctggcgaagg acttggagca
     4861 gcaatcgaaa gcgcagcagc agagtaccgg cgagatgctg aaagccgcat tcagcgagca
     4921 cgagaaatct gtaaaagcgg aactgagcgc aagcgcgaag agaatcagcg acgccatcag
     4981 cgcccacgag cagggtatga ccgcggcgat gcagtcgaac cgcctgagcg tgctgcgtat
     5041 ggtcggccgg acgtggctga ccatcaccct ggtctcggta ctgctgatcg ccatgagcgg
     5101 gagcattctg tggtggcagg ggcagcagat caccgggaat taccagacta tccgggcgca
     5161 ggagcggacg caggccatgt tgtcggagaa aaaccacggc gttcagctgt cgctctgcgg
     5221 ggagaagaaa ctgtcctgcg tcaaagtgaa cccgaaagcg ggagcttacg gggaggaagg
     5281 gaactggatg gtactggaga ggaaatagcc catgacggaa ctggaaaaac agttgctgac
     5341 cgcattagag cagctacatc aggactactc gcaaaggctg gacgagtggg agagcgcctt
     5401 cgcggaatgg cagaagatgt ctggtcttat gcaacgggag aacgcggcgc tgaacgagcg
     5461 cgtcacgcgc ttgagtcagc aggtggcgaa cttgagcagg cagttacagc gtttgagcca
     5521 gtagtgcagc ggcatgaaat ggcggtggcc gccgaacggg catacgagca gcgacagcac
     5581 gaaaaagagc tggcggaagc ccgttcccgt cagcggagct ataacggtcc gacgctgggc
     5641 tgacaggctt caccagtgac aaaccccacg aagaagggct cataaatttc ccgctaaata
     5701 cggtcgtgat gaaaacatca cggcgtatac aacggaggga ttatgagact gtttaacgtg
     5761 attgagacgg tgctgaactg cctgtacatg gcgctggaga tctgggactg gctggagaag
     5821 cattttcctg tttttaaggc gttcatccag attctggcct gaaatcagac cgtatgcatc
     5881 tggctgcatg accatgcagc cgggtaaaat ttatctgtca cgaacggggc ggtgtatcgg
     5941 gtggtttgtt gccggtttta cctgactgcc gccccgtttg tgacgaacat gtccggagcg
     6001 gtgcgggcaa gagatgttat ggtaaatcat attagttctg aatgattcgg gctccaccag
     6061 tcattactat ttgagcacca cctgacattt ttatacttgt accctggcta gtcattgtcg
     6121 tatcaccttg tatgatcgtt tttccagtag catgttgacc tccaccagtg actaaaattg
     6181 acgcttgtac ttgagatcca tttatcaaaa cagttccggc ttggtgatca aattttattc
     6241 cgctcaatgc ttttaattca gctccatcta gctgaatttc accattggct gctgcagcct
     6301 gaatggcccc ctgcatctga gaaaataatt tatcaacttc gtcaaaaggt aatggtgttt
     6361 caagcagtga ggcttgttgt tcaacttcag gatgtttact tgctaacttc cctatgatgc
     6421 ctttgattgc atcagaagta agattgctta atgcgccagt gagaacggca agtagtgtgg
     6481 tttcaatcat tatatttccc tcgttagatt gctgtgatgc gtattattta ctctttataa
     6541 tctccgattc tgtccatatg gttagttgca ctattacgtt tataagtgcc acttttacca
     6601 tatatatatc caacgctaca ttgtggacat tttggcgtct cgtcattatg agtgaaattt
     6661 atttctcctc cgacagatag ggtttttacg ttttccggat aatctaatgt agccacgccg
     6721 gtccattcac aattcgagca gacaacatcg aaagatgttg ggatactctt gcagttgatg
     6781 ttgatttgca tatgcataac ctcgattttg cacagaaatt acagtgttat tagttgcata
     6841 ccgactataa cctgagtttg tgatggagtg gtgttttttt gctttgactt gagtaagaga
     6901 aagagatgat cttcttgaga taatctcttt ctgagcgtag tcttttgctc tgtaaacgaa
     6961 aaaaccaccc tggcaggtgg tttttcgaag gttagttaat cctggcagat ttactaaccg
     7021 tggtaacagt cttgtgcgag acatgtcacc aaagctgtcc ttccagtgta gccgcagtaa
     7081 ggccaccact tcaagaactc tgaatacatc tctcgcacat cctgtttacc agtggctact
     7141 gccaatggcg atttgtcgtg ccttttcggg ttggactcaa gacgatagtt accgaaaaga
     7201 gcgtggtagt cggactgaac ggggggatcg tgcatacagt ccagcttgga gcgaactgcc
     7261 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     7321 accggtaaac cgaatggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc
     7381 ctgggatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattttatg
     7441 atactcgtca ggggggcgga gcctatggaa aaacggcaac gccgcatcct cttcccggct
     7501 tctcctgatt tgagcgctgc ttgcgtatcc cgtcatctgt gagctggtaa cgcccgccgc
     7561 agtctcacgg caggagcgca gcgactgagt gagcgaggag gcgtcatttc acctttacca
     7621 gcatgttgca cttacgcccg tcagccacat tttccccttc ccacatgaag cactttcata
     7681 cattaccact ccgccgtaac atatttagcc agtatacact ccgctagcgc tacgtgactg
     7741 gttcagggct gcgccccgaa acccgctaaa agccactgac gcgcctgcgg cttgtccaac
     7801 accgtgccga ccgggaaaca tttcccgccc gtcccggtgt tatcaggagg ctactctggc
     7861 agacaaacgc aacaagatgc tcacgatgtg ggtgacggaa gacgaacacc ggcggctgct
     7921 cgagcggtgc gacggcaggc agctggcggc gtggatgcgt cagtcctgcc tcgatgagaa
     7981 gccggcgcgt gcctgcagac tgccgtcaat atcgccggcg ctgctgcgcc agcttgccgg
     8041 tatgggtaac aacctgaacc agatcgcccg gaaagtgaat gccgttggtg ccggtcatga
     8101 tcgg
//
