LOCUS       NZ_CP137398             2058 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP119_WM3_21.23_ST147_NDM1 plasmid
            unnamed3, complete sequence.
ACCESSION   NZ_CP137398
VERSION     NZ_CP137398.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951961
            Assembly: GCF_033253665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 354x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:59:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,867
            CDSs (total)                      :: 5,742
            Genes (coding)                    :: 5,589
            CDSs (with protein)               :: 5,589
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 71 of 153
            Pseudo Genes (incomplete)         :: 88 of 153
            Pseudo Genes (internal stop)      :: 29 of 153
            Pseudo Genes (multiple problems)  :: 28 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137398.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP119_WM3_21.23_ST147_NDM1"
                     /isolation_source="pooled swabs"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-05"
                     /genotype="ST147"
     gene            complement(272..1261)
                     /locus_tag="R5O49_RS26565"
                     /old_locus_tag="R5O49_26560"
     CDS             complement(272..1261)
                     /locus_tag="R5O49_RS26565"
                     /old_locus_tag="R5O49_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_040207214.1"
                     /translation="MQNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIEELGETLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPKWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVKKYRRSPTKDKTESD"
                     /name="protein rep"
     gene            complement(1585..1776)
                     /locus_tag="R5O49_RS26570"
                     /old_locus_tag="R5O49_26565"
     CDS             complement(1585..1776)
                     /locus_tag="R5O49_RS26570"
                     /old_locus_tag="R5O49_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020324555.1"
                     /translation="MIVRLDNRLFEILDEMGAHVDREMFMAAYAQRKAEKENDPRHIRQ
                     RKLTRLAHDLMSESDPEL"
                     /name="hypothetical protein"
     Typing          355..546
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 ccgttatcag tttttgtttt tcgtgggctt gccccaccgc gcgtttctca tcgccagatg
       61 agaaactact aagcgcgcct tccggaaaat taccggaaga aaaacccagg ttcggaatcc
      121 gaactcagta atcgagtgac gtcactttgc ggcgttagcc ctatcctgca tcggagtaaa
      181 atcaatcgga caagatgtga ctttggtaca ccgccaccaa ggtgacggat gcccaaagcc
      241 ctcatggtca gggcgcagcc ccgacaccct gttagtctga ttcggtttta tctttcgtcg
      301 gcgaacgccg gtactttttg acctcggtct tccactcgaa cgcgatgcgg gaaccgtcat
      361 catcgccctc ggaaatatca tccccgatca ccatgtcggc gtcggtttcc tgatcgagtt
      421 tcagcacgtc tttgagtgcg ccgccggtcg ccacaaagcg gcgcttgtgc gtctggcggg
      481 tcagttcgag gaaccattta ggatcggcca ccatgtcggc cggcttcgtg gaatatttca
      541 gggtttcggc cactgcgccg cgcaccagtt cggcggtcgc gcaggccagc gattcgccgt
      601 ccttcggctt gcggggtttc actgcccgga catcgacatt cggatcgtaa ctgacccgca
      661 ggcactcacg ccacagctca acccagcgtt catgcctgac gtaactcttc ccgttaagca
      721 tgcccggcgg aaccatcatc agggtgtgga agtgcgggtg tgcgctgccg tcactgccgc
      781 gggtgacttc cgtggtgcgt atccagccct gaactggtcg gaactcttta cggtccttca
      841 gtcgctggaa agccgtgttc atccggctca gcgtctcgcc cagctcctcg atcgcgcagt
      901 tgcgcacggt cagggtcaga aacatccagc gggaatcggg gtaatccgcc acgatacgcg
      961 gcagggactg gtagaaacgg gcttgccaca tcagggagcg cctccactgg cagacaggac
     1021 agtgccggac acggcagaaa tgcgcctcac gcagcctcag gcgcgtttct ccggtttctt
     1081 tgagggtaga ccacccaaac ctcagtaaac cgccacagga ggccatacgc gccccgtagc
     1141 gctcatattc agccgcaagc aggtaaatcc ctcccacgtc gtcagaaacg cttctgtgag
     1201 cgtcccacgg cgcatctgag ggggagtagt cagttaggtt ctggagttca ggattttgca
     1261 tgcacaccgc cccatgctgg gcgatggctc atttgacgtg ttatcattgc catatcacct
     1321 gtgttgtcgt gtccaaattc gaattcctac aggtggtcac acccccgaac tgttagcgca
     1381 gttgcggggg tcgctttttt ctgcgaccac tcccgacaga atacttccaa aaccattttc
     1441 aggcaaatca cactcaaacc cttgcggcac aagggctggc ggcgtttttt gacgccagag
     1501 ttatccctta agtataaata caaggatcgc gcgcacgaga ctccggccat tttctgaccg
     1561 atttccctcg cgcgcacgcg aggctcacaa ttcagggtcc gattctgaca tcaaatcatg
     1621 agccaacctg gtcagtttcc gttgacggat atgccgggga tcattttctt tttcggcctt
     1681 ccgctgcgca taggccgcca tgaacatttc ccgatccaca tgcgcaccca tctcatcgag
     1741 aatctcaaac agccggttat ccagtctgac gatcatgcct accccctcaa aaaacgctta
     1801 tcaggtggtt gcgctcactg cgtgatcaca accaccccct gtaagtattt tacgggtgct
     1861 gtcgtcaatc actaaatcgg aacccactgg caagccagcg ggaccccgcg atttagcgtt
     1921 tgacgatttc gggaacggcg aacgctgaca gattttaatc tggcggcgtg aggtgttggc
     1981 agggtgtcgg ggcgcagccc tgacggatcg tttttggtac cggcatcgtg atggcggtac
     2041 tccaaaaaca catcttgt
//
