LOCUS       NZ_CP137395             8334 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP124_L5_21.23_ST147_NDM5 plasmid
            unnamed2, complete sequence.
ACCESSION   NZ_CP137395
VERSION     NZ_CP137395.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951962
            Assembly: GCF_033253045.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 13:04:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,719
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 80 of 173
            Pseudo Genes (incomplete)         :: 93 of 173
            Pseudo Genes (internal stop)      :: 30 of 173
            Pseudo Genes (multiple problems)  :: 25 of 173
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137395.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP124_L5_21.23_ST147_NDM5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-05"
                     /genotype="ST147"
     gene            complement(174..575)
                     /locus_tag="R5O50_RS29150"
                     /old_locus_tag="R5O50_29150"
     CDS             complement(174..575)
                     /locus_tag="R5O50_RS29150"
                     /old_locus_tag="R5O50_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(575..766)
                     /locus_tag="R5O50_RS29155"
                     /old_locus_tag="R5O50_29155"
     CDS             complement(575..766)
                     /locus_tag="R5O50_RS29155"
                     /old_locus_tag="R5O50_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1182..1295
                     /locus_tag="R5O50_RS29160"
                     /old_locus_tag="R5O50_29160"
                     /pseudo=""
     CDS             1182..1295
                     /locus_tag="R5O50_RS29160"
                     /old_locus_tag="R5O50_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1640..2755
                     /locus_tag="R5O50_RS29165"
                     /old_locus_tag="R5O50_29165"
     CDS             1640..2755
                     /locus_tag="R5O50_RS29165"
                     /old_locus_tag="R5O50_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            2755..3387
                     /locus_tag="R5O50_RS29170"
                     /old_locus_tag="R5O50_29170"
     CDS             2755..3387
                     /locus_tag="R5O50_RS29170"
                     /old_locus_tag="R5O50_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            3753..3995
                     /locus_tag="R5O50_RS29175"
                     /old_locus_tag="R5O50_29175"
     CDS             3753..3995
                     /locus_tag="R5O50_RS29175"
                     /old_locus_tag="R5O50_29175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(4341..4742)
                     /locus_tag="R5O50_RS29180"
                     /old_locus_tag="R5O50_29180"
     CDS             complement(4341..4742)
                     /locus_tag="R5O50_RS29180"
                     /old_locus_tag="R5O50_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4742..4933)
                     /locus_tag="R5O50_RS29185"
                     /old_locus_tag="R5O50_29185"
     CDS             complement(4742..4933)
                     /locus_tag="R5O50_RS29185"
                     /old_locus_tag="R5O50_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5349..5462
                     /locus_tag="R5O50_RS29190"
                     /old_locus_tag="R5O50_29190"
                     /pseudo=""
     CDS             5349..5462
                     /locus_tag="R5O50_RS29190"
                     /old_locus_tag="R5O50_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            5807..6922
                     /locus_tag="R5O50_RS29195"
                     /old_locus_tag="R5O50_29195"
     CDS             5807..6922
                     /locus_tag="R5O50_RS29195"
                     /old_locus_tag="R5O50_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            6922..7554
                     /locus_tag="R5O50_RS29200"
                     /old_locus_tag="R5O50_29200"
     CDS             6922..7554
                     /locus_tag="R5O50_RS29200"
                     /old_locus_tag="R5O50_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            7920..8162
                     /locus_tag="R5O50_RS29205"
                     /old_locus_tag="R5O50_29205"
     CDS             7920..8162
                     /locus_tag="R5O50_RS29205"
                     /old_locus_tag="R5O50_29205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          888..1295
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          5055..5462
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gcacatttat ttttcattaa cccattgttg taaataagga atctgcaaaa ttttaatttt
       61 gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctgacg atttttggga
      121 aggttcagga actccctggg atggccggag aggaaaactg aagcatgacg gggtcaggat
      181 tttttccaga atttccacca tggcgccggg gattcagtta attttgcctg gtgattttca
      241 agaaggagca tggcctgttt caggctgtca atgtgcgaat ccttggacaa aagtaaggtg
      301 ttcaggtgtt caatctcctg tttcagatga cggatcacct gttcactctg gtcagcagag
      361 gctgtatcac gctgtccaga gacctgtcca gaatggtgtt cacccttcgg ttgctcaaag
      421 gggccaaaga cacgtaaaag ctctgaagta tctaccattt ttgcattgcg ctcaccgcta
      481 caggtggaca ggtcacctgc tttaattaac ctgtgcagtg ttgttctact cttacccgtc
      541 agtcgggcag cctcactaat agacactctg gccatcagtt accatcctgt aatgcatcca
      601 gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt
      661 cggcctcgac atcgagatca agctcgttaa gcttctccag caggagcaga gactgattct
      721 ggatgaattt agccatgttc agcgctgttt tctgttgttt cgtcataaga ggcctcagag
      781 cgtcagtggt cagtgtaggg atctcctctt ttccgcttcc tcgctcactc agtcgctacg
      841 ctcctgccgt tcggctgcgg cgagcggcag gagcatgcgt agaaacgctc gagaaaaccg
      901 agtaatcgca tggaggaaag aaggaaaggc cgcacagcgg ccgtttttcc ataggctccg
      961 cccccctgac gagcatcacg gaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     1021 actataaaga taccaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc
     1081 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga
     1141 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
     1201 agtccgactg ctgcgcctaa tccggtaact atcgtcttga gtccaacccg gaaagacacg
     1261 acaaagcgcc actggcagca gccactggta actgatttag ggagttagtc ttgaagttat
     1321 acgccagtta aagctaaact aaacgaacag ttttggtgac tgcgctccct ttaagccagt
     1381 tacctcggtt catagagcgc caactctact gaacctttcg aaaaaaccgc ccgccaaggc
     1441 ggtttttttc gttcacagcc ggaagcttac ggctgctgcc agataatctc aagaagaaag
     1501 taaaaaaaat cgattaaaac acaagcaatc aaagttaatc tttgacataa agtctagcta
     1561 atcacaatga aagctgtaca ttttattaag gtactaacaa atcgtatggc atataacaaa
     1621 aacacgaaca gaggctatca tgataaagga aataagttta aagaacttca aatgcttcaa
     1681 tgagctatat ctaaagcaat taaagacttt aaatatcata gctgggaaaa acaactacgg
     1741 gaaaacatct atactggatg ctattttttg tttttatgat gtaaaaaacc cggctgtttt
     1801 gttaaacatt caagcattca gaaaagaaat ggcagaaata aacaaaaaca agccattttg
     1861 ggttagttac tttcatgata tggacacatc acaaaagatg tccatagtca ttcgagatga
     1921 acgctctgaa gtaacacaaa catatgaaac agaaacaaat cagagattag aatcaagttt
     1981 gtctctaaac gttgacaccg tactatctaa ccaaaacata cctcgtcaaa ctatcggttc
     2041 tcaggccaca gtaaactctt taaaaataaa tgtgaccgag acacccaatg cgaaaaacag
     2101 ggtttccaat aagcttttct ctatgcaatt gaaagaagat ggcgtagaaa taaaatccga
     2161 aataaaaaac gaaagggatg ggattaatta taaatttaag acagcaacaa taataacaac
     2221 atccagaaag ataaacaaag aggcaacaat tacaaatgta tctagcctac taacacaaaa
     2281 aaggaaaaaa gacatactag aaaacttaaa aaaaatagat gacagaattg tagatatagc
     2341 aatatctgcg ataggtaaca ataaggaaat atatttagac attggattta gtgagctaaa
     2401 tgaaatatca atgcttggcg agggtatcag tcgagcatta tcttttatca gctcagtgct
     2461 ggtacaggaa aactcaatta ttcttattga tgaaatcgag aacggaatac attactcagt
     2521 aattaaagac ataataaaat cattaatttc atctgcaaaa caaaacaaca atcaaatatt
     2581 tgcaacaact catagtcaag atgtgatacg agcaataaac gaaatagata gcaaaaatga
     2641 agatatcgct tatattagac taggaagaga aaagaactcc ctaaaaccaa cagccgtaca
     2701 attcaatatg gatgatttct cctactcagt tgagaacgac tgggaggtaa gataatgaaa
     2761 gacataaata atagcaaaaa gatcattttc gttgagggtg atgatgattt tcattttcta
     2821 tgcaacctac ttgaaataca aggcattacc gatgtttttg tcgaaaaaat aaatggaaaa
     2881 tcaaaattaa aacaagcact tagagcattc aaaaacattg attctttcga tgaaaaagaa
     2941 agtattttta ttatattaga tgctgacaca tcattttctg acacagaaag atcaataatt
     3001 gccacattgc gagaactaca aatctcctcc cccagctcgc ataacgagat agctatgaat
     3061 ggcaatacaa aaatttcttt ctttataatg ccaggaaaag acaagcaagg tgccattgaa
     3121 gatctgataa ttgaacacgc aagcactcga gcgttatatg aatacataat agaatttttc
     3181 gaaaaaatta aaaataataa agatgacatc tcagcgagtg acaaagagta cacttatcca
     3241 acaaacgaaa aaaaagctaa ggtacaagtc tatcttagct gcaatagaga atccgactca
     3301 agaataggaa tatccatgaa aaatggaata ataaacacag cagacaattg ctttgatgaa
     3361 ataaaagatt ttatttcaaa aatataaatc caaatcaagc cagcccttca cgggctggct
     3421 tatcaccagg gtgttattaa acgccggata acgtatagaa agcatcatac actttagtta
     3481 tcagcaatct caaacgtcct aaagacatca ccagatgcca aaacaatgat tgtgcagatc
     3541 taatgtaaaa gacccagcac aagatgctcc cttaagttat attctctcta ttcacttaaa
     3601 acaatttaac ataaaatcat aattgtacgc accgctaaaa cggactcagg acgtttctga
     3661 cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc
     3721 tgtttttacg gcatgcatga gtatgcgcgc aagtgcatag cgcggaaatt cacaccattc
     3781 agccagggga agtcctgttt ccggcgttct tctcctccga aaacggtgaa cggggacgca
     3841 atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt tcagcacctt
     3901 caggaatcgg gttcgtcgac ggccggcaat cacgttatgg caagtcgccg gacgactcac
     3961 cacacgattc accggcgatt cgaatcgtga attgaatcaa gagtcaccat gagcaaaaaa
     4021 cacccaaagt gatgaattga atcaagagtc gattcatcat aacaatatga taataaagcg
     4081 tgattcacat atgaatcgtg ggcaattcgt cagcgattcg ccatagagtc gtccacgact
     4141 tggatctgca ctttgtaaca catcaccgca catttatttt tcattaaccc attgttgtaa
     4201 ataaggaatc tgcaaaattt taattttgcg tggggtgtgg gtatttttag tggtgaggag
     4261 cgttcagaga tctgacgatt tttgggaagg ttcaggaact ccctgggatg gccggagagg
     4321 aaaactgaag catgacgggg tcaggatttt ttccagaatt tccaccatgg cgccggggat
     4381 tcagttaatt ttgcctggtg attttcaaga aggagcatgg cctgtttcag gctgtcaatg
     4441 tgcgaatcct tggacaaaag taaggtgttc aggtgttcaa tctcctgttt cagatgacgg
     4501 atcacctgtt cactctggtc agcagaggct gtatcacgct gtccagagac ctgtccagaa
     4561 tggtgttcac ccttcggttg ctcaaagggg ccaaagacac gtaaaagctc tgaagtatct
     4621 accatttttg cattgcgctc accgctacag gtggacaggt cacctgcttt aattaacctg
     4681 tgcagtgttg ttctactctt acccgtcagt cgggcagcct cactaataga cactctggcc
     4741 atcagttacc atcctgtaat gcatccagtc tcgaggacag tgtacggaaa agatgctccg
     4801 catcatcgtg gagtttctca caaaggtcgg cctcgacatc gagatcaagc tcgttaagct
     4861 tctccagcag gagcagagac tgattctgga tgaatttagc catgttcagc gctgttttct
     4921 gttgtttcgt cataagaggc ctcagagcgt cagtggtcag tgtagggatc tcctcttttc
     4981 cgcttcctcg ctcactcagt cgctacgctc ctgccgttcg gctgcggcga gcggcaggag
     5041 catgcgtaga aacgctcgag aaaaccgagt aatcgcatgg aggaaagaag gaaaggccgc
     5101 acagcggccg tttttccata ggctccgccc ccctgacgag catcacggaa tctgacgctc
     5161 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctgatgg
     5221 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     5281 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     5341 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgcctaatcc ggtaactatc
     5401 gtcttgagtc caacccggaa agacacgaca aagcgccact ggcagcagcc actggtaact
     5461 gatttaggga gttagtcttg aagttatacg ccagttaaag ctaaactaaa cgaacagttt
     5521 tggtgactgc gctcccttta agccagttac ctcggttcat agagcgccaa ctctactgaa
     5581 cctttcgaaa aaaccgcccg ccaaggcggt ttttttcgtt cacagccgga agcttacggc
     5641 tgctgccaga taatctcaag aagaaagtaa aaaaaatcga ttaaaacaca agcaatcaaa
     5701 gttaatcttt gacataaagt ctagctaatc acaatgaaag ctgtacattt tattaaggta
     5761 ctaacaaatc gtatggcata taacaaaaac acgaacagag gctatcatga taaaggaaat
     5821 aagtttaaag aacttcaaat gcttcaatga gctatatcta aagcaattaa agactttaaa
     5881 tatcatagct gggaaaaaca actacgggaa aacatctata ctggatgcta ttttttgttt
     5941 ttatgatgta aaaaacccgg ctgttttgtt aaacattcaa gcattcagaa aagaaatggc
     6001 agaaataaac aaaaacaagc cattttgggt tagttacttt catgatatgg acacatcaca
     6061 aaagatgtcc atagtcattc gagatgaacg ctctgaagta acacaaacat atgaaacaga
     6121 aacaaatcag agattagaat caagtttgtc tctaaacgtt gacaccgtac tatctaacca
     6181 aaacatacct cgtcaaacta tcggttctca ggccacagta aactctttaa aaataaatgt
     6241 gaccgagaca cccaatgcga aaaacagggt ttccaataag cttttctcta tgcaattgaa
     6301 agaagatggc gtagaaataa aatccgaaat aaaaaacgaa agggatggga ttaattataa
     6361 atttaagaca gcaacaataa taacaacatc cagaaagata aacaaagagg caacaattac
     6421 aaatgtatct agcctactaa cacaaaaaag gaaaaaagac atactagaaa acttaaaaaa
     6481 aatagatgac agaattgtag atatagcaat atctgcgata ggtaacaata aggaaatata
     6541 tttagacatt ggatttagtg agctaaatga aatatcaatg cttggcgagg gtatcagtcg
     6601 agcattatct tttatcagct cagtgctggt acaggaaaac tcaattattc ttattgatga
     6661 aatcgagaac ggaatacatt actcagtaat taaagacata ataaaatcat taatttcatc
     6721 tgcaaaacaa aacaacaatc aaatatttgc aacaactcat agtcaagatg tgatacgagc
     6781 aataaacgaa atagatagca aaaatgaaga tatcgcttat attagactag gaagagaaaa
     6841 gaactcccta aaaccaacag ccgtacaatt caatatggat gatttctcct actcagttga
     6901 gaacgactgg gaggtaagat aatgaaagac ataaataata gcaaaaagat cattttcgtt
     6961 gagggtgatg atgattttca ttttctatgc aacctacttg aaatacaagg cattaccgat
     7021 gtttttgtcg aaaaaataaa tggaaaatca aaattaaaac aagcacttag agcattcaaa
     7081 aacattgatt ctttcgatga aaaagaaagt atttttatta tattagatgc tgacacatca
     7141 ttttctgaca cagaaagatc aataattgcc acattgcgag aactacaaat ctcctccccc
     7201 agctcgcata acgagatagc tatgaatggc aatacaaaaa tttctttctt tataatgcca
     7261 ggaaaagaca agcaaggtgc cattgaagat ctgataattg aacacgcaag cactcgagcg
     7321 ttatatgaat acataataga atttttcgaa aaaattaaaa ataataaaga tgacatctca
     7381 gcgagtgaca aagagtacac ttatccaaca aacgaaaaaa aagctaaggt acaagtctat
     7441 cttagctgca atagagaatc cgactcaaga ataggaatat ccatgaaaaa tggaataata
     7501 aacacagcag acaattgctt tgatgaaata aaagatttta tttcaaaaat ataaatccaa
     7561 atcaagccag cccttcacgg gctggcttat caccagggtg ttattaaacg ccggataacg
     7621 tatagaaagc atcatacact ttagttatca gcaatctcaa acgtcctaaa gacatcacca
     7681 gatgccaaaa caatgattgt gcagatctaa tgtaaaagac ccagcacaag atgctccctt
     7741 aagttatatt ctctctattc acttaaaaca atttaacata aaatcataat tgtacgcacc
     7801 gctaaaacgg actcaggacg tttctgacgg gaaaccggta aaacaggcaa caaaccaccc
     7861 gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta tgcgcgcaag
     7921 tgcatagcgc ggaaattcac accattcagc caggggaagt cctgtttccg gcgttcttct
     7981 cctccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc
     8041 agccggaaaa ccggttttca gcaccttcag gaatcgggtt cgtcgacggc cggcaatcac
     8101 gttatggcaa gtcgccggac gactcaccac acgattcacc ggcgattcga atcgtgaatt
     8161 gaatcaagag tcaccatgag caaaaaacac ccaaagtgat gaattgaatc aagagtcgat
     8221 tcatcataac aatatgataa taaagcgtga ttcacatatg aatcgtgggc aattcgtcag
     8281 cgattcgcca tagagtcgtc cacgacttgg atctgcactt tgtaacacat cacc
//
