LOCUS       NZ_CP137390             4167 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP39_L11_43.22_ST147_NDM5 plasmid
            unnamed2, complete sequence.
ACCESSION   NZ_CP137390
VERSION     NZ_CP137390.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951963
            Assembly: GCF_033252145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 204x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 13:02:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,814
            CDSs (total)                      :: 5,690
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 69 of 154
            Pseudo Genes (incomplete)         :: 90 of 154
            Pseudo Genes (internal stop)      :: 26 of 154
            Pseudo Genes (multiple problems)  :: 26 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137390.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP39_L11_43.22_ST147_NDM5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-10"
                     /genotype="ST147"
     gene            complement(join(3937..4167,1..402))
                     /locus_tag="R5O51_RS29045"
                     /old_locus_tag="R5O51_29050"
     CDS             complement(join(3937..4167,1..402))
                     /locus_tag="R5O51_RS29045"
                     /old_locus_tag="R5O51_29050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(402..1517)
                     /locus_tag="R5O51_RS29050"
                     /old_locus_tag="R5O51_29055"
     CDS             complement(402..1517)
                     /locus_tag="R5O51_RS29050"
                     /old_locus_tag="R5O51_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(1862..1975)
                     /locus_tag="R5O51_RS29055"
                     /old_locus_tag="R5O51_29060"
                     /pseudo=""
     CDS             complement(1862..1975)
                     /locus_tag="R5O51_RS29055"
                     /old_locus_tag="R5O51_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2391..2582
                     /locus_tag="R5O51_RS29060"
                     /old_locus_tag="R5O51_29065"
     CDS             2391..2582
                     /locus_tag="R5O51_RS29060"
                     /old_locus_tag="R5O51_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2582..2983
                     /locus_tag="R5O51_RS29065"
                     /old_locus_tag="R5O51_29070"
     CDS             2582..2983
                     /locus_tag="R5O51_RS29065"
                     /old_locus_tag="R5O51_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3329..3571)
                     /locus_tag="R5O51_RS29070"
                     /old_locus_tag="R5O51_29075"
     CDS             complement(3329..3571)
                     /locus_tag="R5O51_RS29070"
                     /old_locus_tag="R5O51_29075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(1863..2270)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 taacgctcga gtgcttgcgt gttcaattat cagatcttca atggcacctt gcttgtcttt
       61 tcctggcatt ataaagaaag aaatttttgt attgccattc atagctatct cgttatgcga
      121 gctgggggag gagatttgta gttctcgcaa tgtggcaatt attgatcttt ctgtgtcaga
      181 aaatgatgtg tcagcatcta atataataaa aatactttct ttttcatcga aagaatcaat
      241 gtttttgaat gctctaagtg cttgttttaa ttttgatttt ccatttattt tttcgacaaa
      301 aacatcggta atgccttgta tttcaagtag gttgcataga aaatgaaaat catcatcacc
      361 ctcaacgaaa atgatctttt tgctattatt tatgtctttc attatcttac ctcccagtcg
      421 ttctcaactg agtaggagaa atcatccata ttgaattgta cggctgttgg ttttagggag
      481 ttcttttctc ttcctagtct aatataagcg atatcttcat ttttgctatc tatttcgttt
      541 attgctcgta tcacatcttg actatgagtt gttgcaaata tttgattgtt gttttgtttt
      601 gcagatgaaa ttaatgattt tattatgtct ttaattactg agtaatgtat tccgttctcg
      661 atttcatcaa taagaataat tgagttttcc tgtaccagca ctgagctgat aaaagataat
      721 gctcgactga taccctcgcc aagcattgat atttcattta gctcactaaa tccaatgtct
      781 aaatatattt ccttattgtt acctatcgca gatattgcta tatctacaat tctgtcatct
      841 atttttttta agttttctag tatgtctttt ttcctttttt gtgttagtag gctagataca
      901 tttgtaattg ttgcctcttt gtttatcttt ctggatgttg ttattattgt tgctgtctta
      961 aatttataat taatcccatc cctttcgttt tttatttcgg attttatttc tacgccatct
     1021 tctttcaatt gcatagagaa aagcttattg gaaaccctgt ttttcgcatt gggtgtctcg
     1081 gtcacattta tttttaaaga gtttactgtg gcctgagaac cgatagtttg acgaggtatg
     1141 ttttggttag atagtacggt gtcaacgttt agagacaaac ttgattctaa tctctgattt
     1201 gtttctgttt catatgtttg tgttacttca gagcgttcat ctcgaatgac tatggacatc
     1261 ttttgtgatg tgtccatatc atgaaagtaa ctaacccaaa atggcttgtt tttgtttatt
     1321 tctgccattt cttttctgaa tgcttgaatg tttaacaaaa cagccgggtt ttttacatca
     1381 taaaaacaaa aaatagcatc cagtatagat gttttcccgt agttgttttt cccagctatg
     1441 atatttaaag tctttaattg ctttagatat agctcattga agcatttgaa gttctttaaa
     1501 cttatttcct ttatcatgat agcctctgtt cgtgtttttg ttatatgcca tacgatttgt
     1561 tagtacctta ataaaatgta cagctttcat tgtgattagc tagactttat gtcaaagatt
     1621 aactttgatt gcttgtgttt taatcgattt tttttacttt cttcttgaga ttatctggca
     1681 gcagccgtaa gcttccggct gtgaacgaaa aaaaccgcct tggcgggcgg ttttttcgaa
     1741 aggttcagta gagttggcgc tctatgaacc gaggtaactg gcttaaaggg agcgcagtca
     1801 ccaaaactgt tcgtttagtt tagctttaac tggcgtataa cttcaagact aactccctaa
     1861 atcagttacc agtggctgct gccagtggcg ctttgtcgtg tctttccggg ttggactcaa
     1921 gacgatagtt accggattag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
     1981 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc
     2041 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     2101 gagccatcag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     2161 tttgagcgtc agattccgtg atgctcgtca ggggggcgga gcctatggaa aaacggccgc
     2221 tgtgcggcct ttccttcttt cctccatgcg attactcggt tttctcgagc gtttctacgc
     2281 atgctcctgc cgctcgccgc agccgaacgg caggagcgta gcgactgagt gagcgaggaa
     2341 gcggaaaaga ggagatccct acactgacca ctgacgctct gaggcctctt atgacgaaac
     2401 aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg
     2461 agaagcttaa cgagcttgat ctcgatgtcg aggccgacct ttgtgagaaa ctccacgatg
     2521 atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag gatggtaact
     2581 gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta gaacaacact
     2641 gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgagcgca atgcaaaaat
     2701 ggtagatact tcagagcttt tacgtgtctt tggccccttt gagcaaccga agggtgaaca
     2761 ccattctgga caggtctctg gacagcgtga tacagcctct gctgaccaga gtgaacaggt
     2821 gatccgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt ccaaggattc
     2881 gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg caaaattaac
     2941 tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgaccccgtc atgcttcagt
     3001 tttcctctcc ggccatccca gggagttcct gaaccttccc aaaaatcgtc agatctctga
     3061 acgctcctca ccactaaaaa tacccacacc ccacgcaaaa ttaaaatttt gcagattcct
     3121 tatttacaac aatgggttaa tgaaaaataa atgtgcggtg atgtgttaca aagtgcagat
     3181 ccaagtcgtg gacgactcta tggcgaatcg ctgacgaatt gcccacgatt catatgtgaa
     3241 tcacgcttta ttatcatatt gttatgatga atcgactctt gattcaattc atcactttgg
     3301 gtgttttttg ctcatggtga ctcttgattc aattcacgat tcgaatcgcc ggtgaatcgt
     3361 gtggtgagtc gtccggcgac ttgccataac gtgattgccg gccgtcgacg aacccgattc
     3421 ctgaaggtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata
     3481 aattgcgtcc ccgttcaccg ttttcggagg agaagaacgc cggaaacagg acttcccctg
     3541 gctgaatggt gtgaatttcc gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa
     3601 acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc
     3661 ccgtcagaaa cgtcctgagt ccgttttagc ggtgcgtaca attatgattt tatgttaaat
     3721 tgttttaagt gaatagagag aatataactt aagggagcat cttgtgctgg gtcttttaca
     3781 ttagatctgc acaatcattg ttttggcatc tggtgatgtc tttaggacgt ttgagattgc
     3841 tgataactaa agtgtatgat gctttctata cgttatccgg cgtttaataa caccctggtg
     3901 ataagccagc ccgtgaaggg ctggcttgat ttggatttat atttttgaaa taaaatcttt
     3961 tatttcatca aagcaattgt ctgctgtgtt tattattcca tttttcatgg atattcctat
     4021 tcttgagtcg gattctctat tgcagctaag atagacttgt accttagctt ttttttcgtt
     4081 tgttggataa gtgtactctt tgtcactcgc tgagatgtca tctttattat ttttaatttt
     4141 ttcgaaaaat tctattatgt attcata
//
