LOCUS       NZ_CP137384             8334 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP51_L9_46.22_ST147_NDM1_OXA48 plasmid
            unnamed1, complete sequence.
ACCESSION   NZ_CP137384
VERSION     NZ_CP137384.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951964
            Assembly: GCF_033251225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 13:24:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,956
            CDSs (total)                      :: 5,832
            Genes (coding)                    :: 5,638
            CDSs (with protein)               :: 5,638
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 103 of 194
            Pseudo Genes (incomplete)         :: 97 of 194
            Pseudo Genes (internal stop)      :: 28 of 194
            Pseudo Genes (multiple problems)  :: 29 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137384.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP51_L9_46.22_ST147_NDM1_OXA48"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-11"
                     /genotype="ST147"
     gene            join(8218..8334,1..999)
                     /locus_tag="R5O52_RS29515"
                     /old_locus_tag="R5O52_29510"
     CDS             join(8218..8334,1..999)
                     /locus_tag="R5O52_RS29515"
                     /old_locus_tag="R5O52_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            999..1631
                     /locus_tag="R5O52_RS29520"
                     /old_locus_tag="R5O52_29515"
     CDS             999..1631
                     /locus_tag="R5O52_RS29520"
                     /old_locus_tag="R5O52_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            1997..2239
                     /locus_tag="R5O52_RS29525"
                     /old_locus_tag="R5O52_29520"
     CDS             1997..2239
                     /locus_tag="R5O52_RS29525"
                     /old_locus_tag="R5O52_29520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2585..2986)
                     /locus_tag="R5O52_RS29530"
                     /old_locus_tag="R5O52_29525"
     CDS             complement(2585..2986)
                     /locus_tag="R5O52_RS29530"
                     /old_locus_tag="R5O52_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2986..3177)
                     /locus_tag="R5O52_RS29535"
                     /old_locus_tag="R5O52_29530"
     CDS             complement(2986..3177)
                     /locus_tag="R5O52_RS29535"
                     /old_locus_tag="R5O52_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3593..3706
                     /locus_tag="R5O52_RS29540"
                     /old_locus_tag="R5O52_29535"
                     /pseudo=""
     CDS             3593..3706
                     /locus_tag="R5O52_RS29540"
                     /old_locus_tag="R5O52_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4051..5166
                     /locus_tag="R5O52_RS29545"
                     /old_locus_tag="R5O52_29540"
     CDS             4051..5166
                     /locus_tag="R5O52_RS29545"
                     /old_locus_tag="R5O52_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            5166..5798
                     /locus_tag="R5O52_RS29550"
                     /old_locus_tag="R5O52_29545"
     CDS             5166..5798
                     /locus_tag="R5O52_RS29550"
                     /old_locus_tag="R5O52_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            6164..6406
                     /locus_tag="R5O52_RS29555"
                     /old_locus_tag="R5O52_29550"
     CDS             6164..6406
                     /locus_tag="R5O52_RS29555"
                     /old_locus_tag="R5O52_29550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(6752..7153)
                     /locus_tag="R5O52_RS29560"
                     /old_locus_tag="R5O52_29555"
     CDS             complement(6752..7153)
                     /locus_tag="R5O52_RS29560"
                     /old_locus_tag="R5O52_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7153..7344)
                     /locus_tag="R5O52_RS29565"
                     /old_locus_tag="R5O52_29560"
     CDS             complement(7153..7344)
                     /locus_tag="R5O52_RS29565"
                     /old_locus_tag="R5O52_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7760..7873
                     /locus_tag="R5O52_RS29570"
                     /old_locus_tag="R5O52_29565"
                     /pseudo=""
     CDS             7760..7873
                     /locus_tag="R5O52_RS29570"
                     /old_locus_tag="R5O52_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          3299..3706
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          7466..7873
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gatgctattt tttgttttta tgatgtaaaa aacccggctg ttttgttaaa cattcaagca
       61 ttcagaaaag aaatggcaga aataaacaaa aacaagccat tttgggttag ttactttcat
      121 gatatggaca catcacaaaa gatgtccata gtcattcgag atgaacgctc tgaagtaaca
      181 caaacatatg aaacagaaac aaatcagaga ttagaatcaa gtttgtctct aaacgttgac
      241 accgtactat ctaaccaaaa catacctcgt caaactatcg gttctcaggc cacagtaaac
      301 tctttaaaaa taaatgtgac cgagacaccc aatgcgaaaa acagggtttc caataagctt
      361 ttctctatgc aattgaaaga agatggcgta gaaataaaat ccgaaataaa aaacgaaagg
      421 gatgggatta attataaatt taagacagca acaataataa caacatccag aaagataaac
      481 aaagaggcaa caattacaaa tgtatctagc ctactaacac aaaaaaggaa aaaagacata
      541 ctagaaaact taaaaaaaat agatgacaga attgtagata tagcaatatc tgcgataggt
      601 aacaataagg aaatatattt agacattgga tttagtgagc taaatgaaat atcaatgctt
      661 ggcgagggta tcagtcgagc attatctttt atcagctcag tgctggtaca ggaaaactca
      721 attattctta ttgatgaaat cgagaacgga atacattact cagtaattaa agacataata
      781 aaatcattaa tttcatctgc aaaacaaaac aacaatcaaa tatttgcaac aactcatagt
      841 caagatgtga tacgagcaat aaacgaaata gatagcaaaa atgaagatat cgcttatatt
      901 agactaggaa gagaaaagaa ctccctaaaa ccaacagccg tacaattcaa tatggatgat
      961 ttctcctact cagttgagaa cgactgggag gtaagataat gaaagacata aataatagca
     1021 aaaagatcat tttcgttgag ggtgatgatg attttcattt tctatgcaac ctacttgaaa
     1081 tacaaggcat taccgatgtt tttgtcgaaa aaataaatgg aaaatcaaaa ttaaaacaag
     1141 cacttagagc attcaaaaac attgattctt tcgatgaaaa agaaagtatt tttattatat
     1201 tagatgctga cacatcattt tctgacacag aaagatcaat aattgccaca ttgcgagaac
     1261 tacaaatctc ctcccccagc tcgcataacg agatagctat gaatggcaat acaaaaattt
     1321 ctttctttat aatgccagga aaagacaagc aaggtgccat tgaagatctg ataattgaac
     1381 acgcaagcac tcgagcgtta tatgaataca taatagaatt tttcgaaaaa attaaaaata
     1441 ataaagatga catctcagcg agtgacaaag agtacactta tccaacaaac gaaaaaaaag
     1501 ctaaggtaca agtctatctt agctgcaata gagaatccga ctcaagaata ggaatatcca
     1561 tgaaaaatgg aataataaac acagcagaca attgctttga tgaaataaaa gattttattt
     1621 caaaaatata aatccaaatc aagccagccc ttcacgggct ggcttatcac cagggtgtta
     1681 ttaaacgccg gataacgtat agaaagcatc atacacttta gttatcagca atctcaaacg
     1741 tcctaaagac atcaccagat gccaaaacaa tgattgtgca gatctaatgt aaaagaccca
     1801 gcacaagatg ctcccttaag ttatattctc tctattcact taaaacaatt taacataaaa
     1861 tcataattgt acgcaccgct aaaacggact caggacgttt ctgacgggaa accggtaaaa
     1921 caggcaacaa accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc
     1981 atgagtatgc gcgcaagtgc atagcgcgga aattcacacc attcagccag gggaagtcct
     2041 gtttccggcg ttcttctcct ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg
     2101 tgaagcgttg ccggcgcagc cggaaaaccg gttttcagca ccttcaggaa tcgggttcgt
     2161 cgacggccgg caatcacgtt atggcaagtc gccggacgac tcaccacacg attcaccggc
     2221 gattcgaatc gtgaattgaa tcaagagtca ccatgagcaa aaaacaccca aagtgatgaa
     2281 ttgaatcaag agtcgattca tcataacaat atgataataa agcgtgattc acatatgaat
     2341 cgtgggcaat tcgtcagcga ttcgccatag agtcgtccac gacttggatc tgcactttgt
     2401 aacacatcac cgcacattta tttttcatta acccattgtt gtaaataagg aatctgcaaa
     2461 attttaattt tgcgtggggt gtgggtattt ttagtggtga ggagcgttca gagatctgac
     2521 gatttttggg aaggttcagg aactccctgg gatggccgga gaggaaaact gaagcatgac
     2581 ggggtcagga ttttttccag aatttccacc atggcgccgg ggattcagtt aattttgcct
     2641 ggtgattttc aagaaggagc atggcctgtt tcaggctgtc aatgtgcgaa tccttggaca
     2701 aaagtaaggt gttcaggtgt tcaatctcct gtttcagatg acggatcacc tgttcactct
     2761 ggtcagcaga ggctgtatca cgctgtccag agacctgtcc agaatggtgt tcacccttcg
     2821 gttgctcaaa ggggccaaag acacgtaaaa gctctgaagt atctaccatt tttgcattgc
     2881 gctcaccgct acaggtggac aggtcacctg ctttaattaa cctgtgcagt gttgttctac
     2941 tcttacccgt cagtcgggca gcctcactaa tagacactct ggccatcagt taccatcctg
     3001 taatgcatcc agtctcgagg acagtgtacg gaaaagatgc tccgcatcat cgtggagttt
     3061 ctcacaaagg tcggcctcga catcgagatc aagctcgtta agcttctcca gcaggagcag
     3121 agactgattc tggatgaatt tagccatgtt cagcgctgtt ttctgttgtt tcgtcataag
     3181 aggcctcaga gcgtcagtgg tcagtgtagg gatctcctct tttccgcttc ctcgctcact
     3241 cagtcgctac gctcctgccg ttcggctgcg gcgagcggca ggagcatgcg tagaaacgct
     3301 cgagaaaacc gagtaatcgc atggaggaaa gaaggaaagg ccgcacagcg gccgtttttc
     3361 cataggctcc gcccccctga cgagcatcac ggaatctgac gctcaaatca gtggtggcga
     3421 aacccgacag gactataaag ataccaggcg tttccccctg atggctccct cgtgcgctct
     3481 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     3541 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     3601 accccccgtt cagtccgact gctgcgccta atccggtaac tatcgtcttg agtccaaccc
     3661 ggaaagacac gacaaagcgc cactggcagc agccactggt aactgattta gggagttagt
     3721 cttgaagtta tacgccagtt aaagctaaac taaacgaaca gttttggtga ctgcgctccc
     3781 tttaagccag ttacctcggt tcatagagcg ccaactctac tgaacctttc gaaaaaaccg
     3841 cccgccaagg cggttttttt cgttcacagc cggaagctta cggctgctgc cagataatct
     3901 caagaagaaa gtaaaaaaaa tcgattaaaa cacaagcaat caaagttaat ctttgacata
     3961 aagtctagct aatcacaatg aaagctgtac attttattaa ggtactaaca aatcgtatgg
     4021 catataacaa aaacacgaac agaggctatc atgataaagg aaataagttt aaagaacttc
     4081 aaatgcttca atgagctata tctaaagcaa ttaaagactt taaatatcat agctgggaaa
     4141 aacaactacg ggaaaacatc tatactggat gctatttttt gtttttatga tgtaaaaaac
     4201 ccggctgttt tgttaaacat tcaagcattc agaaaagaaa tggcagaaat aaacaaaaac
     4261 aagccatttt gggttagtta ctttcatgat atggacacat cacaaaagat gtccatagtc
     4321 attcgagatg aacgctctga agtaacacaa acatatgaaa cagaaacaaa tcagagatta
     4381 gaatcaagtt tgtctctaaa cgttgacacc gtactatcta accaaaacat acctcgtcaa
     4441 actatcggtt ctcaggccac agtaaactct ttaaaaataa atgtgaccga gacacccaat
     4501 gcgaaaaaca gggtttccaa taagcttttc tctatgcaat tgaaagaaga tggcgtagaa
     4561 ataaaatccg aaataaaaaa cgaaagggat gggattaatt ataaatttaa gacagcaaca
     4621 ataataacaa catccagaaa gataaacaaa gaggcaacaa ttacaaatgt atctagccta
     4681 ctaacacaaa aaaggaaaaa agacatacta gaaaacttaa aaaaaataga tgacagaatt
     4741 gtagatatag caatatctgc gataggtaac aataaggaaa tatatttaga cattggattt
     4801 agtgagctaa atgaaatatc aatgcttggc gagggtatca gtcgagcatt atcttttatc
     4861 agctcagtgc tggtacagga aaactcaatt attcttattg atgaaatcga gaacggaata
     4921 cattactcag taattaaaga cataataaaa tcattaattt catctgcaaa acaaaacaac
     4981 aatcaaatat ttgcaacaac tcatagtcaa gatgtgatac gagcaataaa cgaaatagat
     5041 agcaaaaatg aagatatcgc ttatattaga ctaggaagag aaaagaactc cctaaaacca
     5101 acagccgtac aattcaatat ggatgatttc tcctactcag ttgagaacga ctgggaggta
     5161 agataatgaa agacataaat aatagcaaaa agatcatttt cgttgagggt gatgatgatt
     5221 ttcattttct atgcaaccta cttgaaatac aaggcattac cgatgttttt gtcgaaaaaa
     5281 taaatggaaa atcaaaatta aaacaagcac ttagagcatt caaaaacatt gattctttcg
     5341 atgaaaaaga aagtattttt attatattag atgctgacac atcattttct gacacagaaa
     5401 gatcaataat tgccacattg cgagaactac aaatctcctc ccccagctcg cataacgaga
     5461 tagctatgaa tggcaataca aaaatttctt tctttataat gccaggaaaa gacaagcaag
     5521 gtgccattga agatctgata attgaacacg caagcactcg agcgttatat gaatacataa
     5581 tagaattttt cgaaaaaatt aaaaataata aagatgacat ctcagcgagt gacaaagagt
     5641 acacttatcc aacaaacgaa aaaaaagcta aggtacaagt ctatcttagc tgcaatagag
     5701 aatccgactc aagaatagga atatccatga aaaatggaat aataaacaca gcagacaatt
     5761 gctttgatga aataaaagat tttatttcaa aaatataaat ccaaatcaag ccagcccttc
     5821 acgggctggc ttatcaccag ggtgttatta aacgccggat aacgtataga aagcatcata
     5881 cactttagtt atcagcaatc tcaaacgtcc taaagacatc accagatgcc aaaacaatga
     5941 ttgtgcagat ctaatgtaaa agacccagca caagatgctc ccttaagtta tattctctct
     6001 attcacttaa aacaatttaa cataaaatca taattgtacg caccgctaaa acggactcag
     6061 gacgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa
     6121 cgcgcaggct ctgtttttac ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat
     6181 tcacaccatt cagccagggg aagtcctgtt tccggcgttc ttctcctccg aaaacggtga
     6241 acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt
     6301 ttcagcacct tcaggaatcg ggttcgtcga cggccggcaa tcacgttatg gcaagtcgcc
     6361 ggacgactca ccacacgatt caccggcgat tcgaatcgtg aattgaatca agagtcacca
     6421 tgagcaaaaa acacccaaag tgatgaattg aatcaagagt cgattcatca taacaatatg
     6481 ataataaagc gtgattcaca tatgaatcgt gggcaattcg tcagcgattc gccatagagt
     6541 cgtccacgac ttggatctgc actttgtaac acatcaccgc acatttattt ttcattaacc
     6601 cattgttgta aataaggaat ctgcaaaatt ttaattttgc gtggggtgtg ggtattttta
     6661 gtggtgagga gcgttcagag atctgacgat ttttgggaag gttcaggaac tccctgggat
     6721 ggccggagag gaaaactgaa gcatgacggg gtcaggattt tttccagaat ttccaccatg
     6781 gcgccgggga ttcagttaat tttgcctggt gattttcaag aaggagcatg gcctgtttca
     6841 ggctgtcaat gtgcgaatcc ttggacaaaa gtaaggtgtt caggtgttca atctcctgtt
     6901 tcagatgacg gatcacctgt tcactctggt cagcagaggc tgtatcacgc tgtccagaga
     6961 cctgtccaga atggtgttca cccttcggtt gctcaaaggg gccaaagaca cgtaaaagct
     7021 ctgaagtatc taccattttt gcattgcgct caccgctaca ggtggacagg tcacctgctt
     7081 taattaacct gtgcagtgtt gttctactct tacccgtcag tcgggcagcc tcactaatag
     7141 acactctggc catcagttac catcctgtaa tgcatccagt ctcgaggaca gtgtacggaa
     7201 aagatgctcc gcatcatcgt ggagtttctc acaaaggtcg gcctcgacat cgagatcaag
     7261 ctcgttaagc ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag
     7321 cgctgttttc tgttgtttcg tcataagagg cctcagagcg tcagtggtca gtgtagggat
     7381 ctcctctttt ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg
     7441 agcggcagga gcatgcgtag aaacgctcga gaaaaccgag taatcgcatg gaggaaagaa
     7501 ggaaaggccg cacagcggcc gtttttccat aggctccgcc cccctgacga gcatcacgga
     7561 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7621 ccccctgatg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     7681 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     7741 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgcctaatc
     7801 cggtaactat cgtcttgagt ccaacccgga aagacacgac aaagcgccac tggcagcagc
     7861 cactggtaac tgatttaggg agttagtctt gaagttatac gccagttaaa gctaaactaa
     7921 acgaacagtt ttggtgactg cgctcccttt aagccagtta cctcggttca tagagcgcca
     7981 actctactga acctttcgaa aaaaccgccc gccaaggcgg tttttttcgt tcacagccgg
     8041 aagcttacgg ctgctgccag ataatctcaa gaagaaagta aaaaaaatcg attaaaacac
     8101 aagcaatcaa agttaatctt tgacataaag tctagctaat cacaatgaaa gctgtacatt
     8161 ttattaaggt actaacaaat cgtatggcat ataacaaaaa cacgaacaga ggctatcatg
     8221 ataaaggaaa taagtttaaa gaacttcaaa tgcttcaatg agctatatct aaagcaatta
     8281 aagactttaa atatcatagc tgggaaaaac aactacggga aaacatctat actg
//
