LOCUS       NZ_CP137369             8334 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP81_L15_03.23_ST147_NDM5 plasmid
            unnamed1, complete sequence.
ACCESSION   NZ_CP137369
VERSION     NZ_CP137369.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951966
            Assembly: GCF_033245165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 159x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 13:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,050
            CDSs (total)                      :: 5,927
            Genes (coding)                    :: 5,748
            CDSs (with protein)               :: 5,748
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 80 of 179
            Pseudo Genes (incomplete)         :: 100 of 179
            Pseudo Genes (internal stop)      :: 30 of 179
            Pseudo Genes (multiple problems)  :: 26 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137369.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP81_L15_03.23_ST147_NDM5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-01"
                     /genotype="ST147"
     gene            join(8220..8334,1..1001)
                     /locus_tag="R5O54_RS00005"
                     /old_locus_tag="R5O54_00005"
     CDS             join(8220..8334,1..1001)
                     /locus_tag="R5O54_RS00005"
                     /old_locus_tag="R5O54_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            1001..1633
                     /locus_tag="R5O54_RS00010"
                     /old_locus_tag="R5O54_00010"
     CDS             1001..1633
                     /locus_tag="R5O54_RS00010"
                     /old_locus_tag="R5O54_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            1999..2241
                     /locus_tag="R5O54_RS00015"
                     /old_locus_tag="R5O54_00015"
     CDS             1999..2241
                     /locus_tag="R5O54_RS00015"
                     /old_locus_tag="R5O54_00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2587..2988)
                     /locus_tag="R5O54_RS00020"
                     /old_locus_tag="R5O54_00020"
     CDS             complement(2587..2988)
                     /locus_tag="R5O54_RS00020"
                     /old_locus_tag="R5O54_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2988..3179)
                     /locus_tag="R5O54_RS00025"
                     /old_locus_tag="R5O54_00025"
     CDS             complement(2988..3179)
                     /locus_tag="R5O54_RS00025"
                     /old_locus_tag="R5O54_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3595..3708
                     /locus_tag="R5O54_RS00030"
                     /old_locus_tag="R5O54_00030"
                     /pseudo=""
     CDS             3595..3708
                     /locus_tag="R5O54_RS00030"
                     /old_locus_tag="R5O54_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4053..5168
                     /locus_tag="R5O54_RS00035"
                     /old_locus_tag="R5O54_00035"
     CDS             4053..5168
                     /locus_tag="R5O54_RS00035"
                     /old_locus_tag="R5O54_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            5168..5800
                     /locus_tag="R5O54_RS00040"
                     /old_locus_tag="R5O54_00040"
     CDS             5168..5800
                     /locus_tag="R5O54_RS00040"
                     /old_locus_tag="R5O54_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            6166..6408
                     /locus_tag="R5O54_RS00045"
                     /old_locus_tag="R5O54_00045"
     CDS             6166..6408
                     /locus_tag="R5O54_RS00045"
                     /old_locus_tag="R5O54_00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(6754..7155)
                     /locus_tag="R5O54_RS00050"
                     /old_locus_tag="R5O54_00050"
     CDS             complement(6754..7155)
                     /locus_tag="R5O54_RS00050"
                     /old_locus_tag="R5O54_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7155..7346)
                     /locus_tag="R5O54_RS00055"
                     /old_locus_tag="R5O54_00055"
     CDS             complement(7155..7346)
                     /locus_tag="R5O54_RS00055"
                     /old_locus_tag="R5O54_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7762..7875
                     /locus_tag="R5O54_RS00060"
                     /old_locus_tag="R5O54_00060"
                     /pseudo=""
     CDS             7762..7875
                     /locus_tag="R5O54_RS00060"
                     /old_locus_tag="R5O54_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          3301..3708
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          7468..7875
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tggatgctat tttttgtttt tatgatgtaa aaaacccggc tgttttgtta aacattcaag
       61 cattcagaaa agaaatggca gaaataaaca aaaacaagcc attttgggtt agttactttc
      121 atgatatgga cacatcacaa aagatgtcca tagtcattcg agatgaacgc tctgaagtaa
      181 cacaaacata tgaaacagaa acaaatcaga gattagaatc aagtttgtct ctaaacgttg
      241 acaccgtact atctaaccaa aacatacctc gtcaaactat cggttctcag gccacagtaa
      301 actctttaaa aataaatgtg accgagacac ccaatgcgaa aaacagggtt tccaataagc
      361 ttttctctat gcaattgaaa gaagatggcg tagaaataaa atccgaaata aaaaacgaaa
      421 gggatgggat taattataaa tttaagacag caacaataat aacaacatcc agaaagataa
      481 acaaagaggc aacaattaca aatgtatcta gcctactaac acaaaaaagg aaaaaagaca
      541 tactagaaaa cttaaaaaaa atagatgaca gaattgtaga tatagcaata tctgcgatag
      601 gtaacaataa ggaaatatat ttagacattg gatttagtga gctaaatgaa atatcaatgc
      661 ttggcgaggg tatcagtcga gcattatctt ttatcagctc agtgctggta caggaaaact
      721 caattattct tattgatgaa atcgagaacg gaatacatta ctcagtaatt aaagacataa
      781 taaaatcatt aatttcatct gcaaaacaaa acaacaatca aatatttgca acaactcata
      841 gtcaagatgt gatacgagca ataaacgaaa tagatagcaa aaatgaagat atcgcttata
      901 ttagactagg aagagaaaag aactccctaa aaccaacagc cgtacaattc aatatggatg
      961 atttctccta ctcagttgag aacgactggg aggtaagata atgaaagaca taaataatag
     1021 caaaaagatc attttcgttg agggtgatga tgattttcat tttctatgca acctacttga
     1081 aatacaaggc attaccgatg tttttgtcga aaaaataaat ggaaaatcaa aattaaaaca
     1141 agcacttaga gcattcaaaa acattgattc tttcgatgaa aaagaaagta tttttattat
     1201 attagatgct gacacatcat tttctgacac agaaagatca ataattgcca cattgcgaga
     1261 actacaaatc tcctccccca gctcgcataa cgagatagct atgaatggca atacaaaaat
     1321 ttctttcttt ataatgccag gaaaagacaa gcaaggtgcc attgaagatc tgataattga
     1381 acacgcaagc actcgagcgt tatatgaata cataatagaa tttttcgaaa aaattaaaaa
     1441 taataaagat gacatctcag cgagtgacaa agagtacact tatccaacaa acgaaaaaaa
     1501 agctaaggta caagtctatc ttagctgcaa tagagaatcc gactcaagaa taggaatatc
     1561 catgaaaaat ggaataataa acacagcaga caattgcttt gatgaaataa aagattttat
     1621 ttcaaaaata taaatccaaa tcaagccagc ccttcacggg ctggcttatc accagggtgt
     1681 tattaaacgc cggataacgt atagaaagca tcatacactt tagttatcag caatctcaaa
     1741 cgtcctaaag acatcaccag atgccaaaac aatgattgtg cagatctaat gtaaaagacc
     1801 cagcacaaga tgctccctta agttatattc tctctattca cttaaaacaa tttaacataa
     1861 aatcataatt gtacgcaccg ctaaaacgga ctcaggacgt ttctgacggg aaaccggtaa
     1921 aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat
     1981 gcatgagtat gcgcgcaagt gcatagcgcg gaaattcaca ccattcagcc aggggaagtc
     2041 ctgtttccgg cgttcttctc ctccgaaaac ggtgaacggg gacgcaattt attgcgtggc
     2101 cgtgaagcgt tgccggcgca gccggaaaac cggttttcag caccttcagg aatcgggttc
     2161 gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg
     2221 gcgattcgaa tcgtgaattg aatcaagagt caccatgagc aaaaaacacc caaagtgatg
     2281 aattgaatca agagtcgatt catcataaca atatgataat aaagcgtgat tcacatatga
     2341 atcgtgggca attcgtcagc gattcgccat agagtcgtcc acgacttgga tctgcacttt
     2401 gtaacacatc accgcacatt tatttttcat taacccattg ttgtaaataa ggaatctgca
     2461 aaattttaat tttgcgtggg gtgtgggtat ttttagtggt gaggagcgtt cagagatctg
     2521 acgatttttg ggaaggttca ggaactccct gggatggccg gagaggaaaa ctgaagcatg
     2581 acggggtcag gattttttcc agaatttcca ccatggcgcc ggggattcag ttaattttgc
     2641 ctggtgattt tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg aatccttgga
     2701 caaaagtaag gtgttcaggt gttcaatctc ctgtttcaga tgacggatca cctgttcact
     2761 ctggtcagca gaggctgtat cacgctgtcc agagacctgt ccagaatggt gttcaccctt
     2821 cggttgctca aaggggccaa agacacgtaa aagctctgaa gtatctacca tttttgcatt
     2881 gcgctcaccg ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gtgttgttct
     2941 actcttaccc gtcagtcggg cagcctcact aatagacact ctggccatca gttaccatcc
     3001 tgtaatgcat ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt
     3061 ttctcacaaa ggtcggcctc gacatcgaga tcaagctcgt taagcttctc cagcaggagc
     3121 agagactgat tctggatgaa tttagccatg ttcagcgctg ttttctgttg tttcgtcata
     3181 agaggcctca gagcgtcagt ggtcagtgta gggatctcct cttttccgct tcctcgctca
     3241 ctcagtcgct acgctcctgc cgttcggctg cggcgagcgg caggagcatg cgtagaaacg
     3301 ctcgagaaaa ccgagtaatc gcatggagga aagaaggaaa ggccgcacag cggccgtttt
     3361 tccataggct ccgcccccct gacgagcatc acggaatctg acgctcaaat cagtggtggc
     3421 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcgtgcgct
     3481 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     3541 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     3601 gaaccccccg ttcagtccga ctgctgcgcc taatccggta actatcgtct tgagtccaac
     3661 ccggaaagac acgacaaagc gccactggca gcagccactg gtaactgatt tagggagtta
     3721 gtcttgaagt tatacgccag ttaaagctaa actaaacgaa cagttttggt gactgcgctc
     3781 cctttaagcc agttacctcg gttcatagag cgccaactct actgaacctt tcgaaaaaac
     3841 cgcccgccaa ggcggttttt ttcgttcaca gccggaagct tacggctgct gccagataat
     3901 ctcaagaaga aagtaaaaaa aatcgattaa aacacaagca atcaaagtta atctttgaca
     3961 taaagtctag ctaatcacaa tgaaagctgt acattttatt aaggtactaa caaatcgtat
     4021 ggcatataac aaaaacacga acagaggcta tcatgataaa ggaaataagt ttaaagaact
     4081 tcaaatgctt caatgagcta tatctaaagc aattaaagac tttaaatatc atagctggga
     4141 aaaacaacta cgggaaaaca tctatactgg atgctatttt ttgtttttat gatgtaaaaa
     4201 acccggctgt tttgttaaac attcaagcat tcagaaaaga aatggcagaa ataaacaaaa
     4261 acaagccatt ttgggttagt tactttcatg atatggacac atcacaaaag atgtccatag
     4321 tcattcgaga tgaacgctct gaagtaacac aaacatatga aacagaaaca aatcagagat
     4381 tagaatcaag tttgtctcta aacgttgaca ccgtactatc taaccaaaac atacctcgtc
     4441 aaactatcgg ttctcaggcc acagtaaact ctttaaaaat aaatgtgacc gagacaccca
     4501 atgcgaaaaa cagggtttcc aataagcttt tctctatgca attgaaagaa gatggcgtag
     4561 aaataaaatc cgaaataaaa aacgaaaggg atgggattaa ttataaattt aagacagcaa
     4621 caataataac aacatccaga aagataaaca aagaggcaac aattacaaat gtatctagcc
     4681 tactaacaca aaaaaggaaa aaagacatac tagaaaactt aaaaaaaata gatgacagaa
     4741 ttgtagatat agcaatatct gcgataggta acaataagga aatatattta gacattggat
     4801 ttagtgagct aaatgaaata tcaatgcttg gcgagggtat cagtcgagca ttatctttta
     4861 tcagctcagt gctggtacag gaaaactcaa ttattcttat tgatgaaatc gagaacggaa
     4921 tacattactc agtaattaaa gacataataa aatcattaat ttcatctgca aaacaaaaca
     4981 acaatcaaat atttgcaaca actcatagtc aagatgtgat acgagcaata aacgaaatag
     5041 atagcaaaaa tgaagatatc gcttatatta gactaggaag agaaaagaac tccctaaaac
     5101 caacagccgt acaattcaat atggatgatt tctcctactc agttgagaac gactgggagg
     5161 taagataatg aaagacataa ataatagcaa aaagatcatt ttcgttgagg gtgatgatga
     5221 ttttcatttt ctatgcaacc tacttgaaat acaaggcatt accgatgttt ttgtcgaaaa
     5281 aataaatgga aaatcaaaat taaaacaagc acttagagca ttcaaaaaca ttgattcttt
     5341 cgatgaaaaa gaaagtattt ttattatatt agatgctgac acatcatttt ctgacacaga
     5401 aagatcaata attgccacat tgcgagaact acaaatctcc tcccccagct cgcataacga
     5461 gatagctatg aatggcaata caaaaatttc tttctttata atgccaggaa aagacaagca
     5521 aggtgccatt gaagatctga taattgaaca cgcaagcact cgagcgttat atgaatacat
     5581 aatagaattt ttcgaaaaaa ttaaaaataa taaagatgac atctcagcga gtgacaaaga
     5641 gtacacttat ccaacaaacg aaaaaaaagc taaggtacaa gtctatctta gctgcaatag
     5701 agaatccgac tcaagaatag gaatatccat gaaaaatgga ataataaaca cagcagacaa
     5761 ttgctttgat gaaataaaag attttatttc aaaaatataa atccaaatca agccagccct
     5821 tcacgggctg gcttatcacc agggtgttat taaacgccgg ataacgtata gaaagcatca
     5881 tacactttag ttatcagcaa tctcaaacgt cctaaagaca tcaccagatg ccaaaacaat
     5941 gattgtgcag atctaatgta aaagacccag cacaagatgc tcccttaagt tatattctct
     6001 ctattcactt aaaacaattt aacataaaat cataattgta cgcaccgcta aaacggactc
     6061 aggacgtttc tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga
     6121 aacgcgcagg ctctgttttt acggcatgca tgagtatgcg cgcaagtgca tagcgcggaa
     6181 attcacacca ttcagccagg ggaagtcctg tttccggcgt tcttctcctc cgaaaacggt
     6241 gaacggggac gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg
     6301 ttttcagcac cttcaggaat cgggttcgtc gacggccggc aatcacgtta tggcaagtcg
     6361 ccggacgact caccacacga ttcaccggcg attcgaatcg tgaattgaat caagagtcac
     6421 catgagcaaa aaacacccaa agtgatgaat tgaatcaaga gtcgattcat cataacaata
     6481 tgataataaa gcgtgattca catatgaatc gtgggcaatt cgtcagcgat tcgccataga
     6541 gtcgtccacg acttggatct gcactttgta acacatcacc gcacatttat ttttcattaa
     6601 cccattgttg taaataagga atctgcaaaa ttttaatttt gcgtggggtg tgggtatttt
     6661 tagtggtgag gagcgttcag agatctgacg atttttggga aggttcagga actccctggg
     6721 atggccggag aggaaaactg aagcatgacg gggtcaggat tttttccaga atttccacca
     6781 tggcgccggg gattcagtta attttgcctg gtgattttca agaaggagca tggcctgttt
     6841 caggctgtca atgtgcgaat ccttggacaa aagtaaggtg ttcaggtgtt caatctcctg
     6901 tttcagatga cggatcacct gttcactctg gtcagcagag gctgtatcac gctgtccaga
     6961 gacctgtcca gaatggtgtt cacccttcgg ttgctcaaag gggccaaaga cacgtaaaag
     7021 ctctgaagta tctaccattt ttgcattgcg ctcaccgcta caggtggaca ggtcacctgc
     7081 tttaattaac ctgtgcagtg ttgttctact cttacccgtc agtcgggcag cctcactaat
     7141 agacactctg gccatcagtt accatcctgt aatgcatcca gtctcgagga cagtgtacgg
     7201 aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt cggcctcgac atcgagatca
     7261 agctcgttaa gcttctccag caggagcaga gactgattct ggatgaattt agccatgttc
     7321 agcgctgttt tctgttgttt cgtcataaga ggcctcagag cgtcagtggt cagtgtaggg
     7381 atctcctctt ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt tcggctgcgg
     7441 cgagcggcag gagcatgcgt agaaacgctc gagaaaaccg agtaatcgca tggaggaaag
     7501 aaggaaaggc cgcacagcgg ccgtttttcc ataggctccg cccccctgac gagcatcacg
     7561 gaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     7621 ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     7681 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     7741 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctaa
     7801 tccggtaact atcgtcttga gtccaacccg gaaagacacg acaaagcgcc actggcagca
     7861 gccactggta actgatttag ggagttagtc ttgaagttat acgccagtta aagctaaact
     7921 aaacgaacag ttttggtgac tgcgctccct ttaagccagt tacctcggtt catagagcgc
     7981 caactctact gaacctttcg aaaaaaccgc ccgccaaggc ggtttttttc gttcacagcc
     8041 ggaagcttac ggctgctgcc agataatctc aagaagaaag taaaaaaaat cgattaaaac
     8101 acaagcaatc aaagttaatc tttgacataa agtctagcta atcacaatga aagctgtaca
     8161 ttttattaag gtactaacaa atcgtatggc atataacaaa aacacgaaca gaggctatca
     8221 tgataaagga aataagttta aagaacttca aatgcttcaa tgagctatat ctaaagcaat
     8281 taaagacttt aaatatcata gctgggaaaa acaactacgg gaaaacatct atac
//
