LOCUS       NZ_CP137368            12500 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP86_L3_06.23_ST147_NDM14 plasmid
            unnamed1, complete sequence.
ACCESSION   NZ_CP137368
VERSION     NZ_CP137368.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951968
            Assembly: GCF_033243425.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12500)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 13:42:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,863
            CDSs (total)                      :: 5,740
            Genes (coding)                    :: 5,533
            CDSs (with protein)               :: 5,533
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 113 of 207
            Pseudo Genes (incomplete)         :: 97 of 207
            Pseudo Genes (internal stop)      :: 30 of 207
            Pseudo Genes (multiple problems)  :: 28 of 207
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137368.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP86_L3_06.23_ST147_NDM14"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-12"
                     /genotype="ST147"
     gene            34..1149
                     /locus_tag="R5O56_RS29230"
                     /old_locus_tag="R5O56_29225"
     CDS             34..1149
                     /locus_tag="R5O56_RS29230"
                     /old_locus_tag="R5O56_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            1149..1781
                     /locus_tag="R5O56_RS29235"
                     /old_locus_tag="R5O56_29230"
     CDS             1149..1781
                     /locus_tag="R5O56_RS29235"
                     /old_locus_tag="R5O56_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            2147..2389
                     /locus_tag="R5O56_RS29240"
                     /old_locus_tag="R5O56_29235"
     CDS             2147..2389
                     /locus_tag="R5O56_RS29240"
                     /old_locus_tag="R5O56_29235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2735..3136)
                     /locus_tag="R5O56_RS29245"
                     /old_locus_tag="R5O56_29240"
     CDS             complement(2735..3136)
                     /locus_tag="R5O56_RS29245"
                     /old_locus_tag="R5O56_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3136..3327)
                     /locus_tag="R5O56_RS29250"
                     /old_locus_tag="R5O56_29245"
     CDS             complement(3136..3327)
                     /locus_tag="R5O56_RS29250"
                     /old_locus_tag="R5O56_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3743..3856
                     /locus_tag="R5O56_RS29255"
                     /old_locus_tag="R5O56_29250"
                     /pseudo=""
     CDS             3743..3856
                     /locus_tag="R5O56_RS29255"
                     /old_locus_tag="R5O56_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4201..5316
                     /locus_tag="R5O56_RS29260"
                     /old_locus_tag="R5O56_29255"
     CDS             4201..5316
                     /locus_tag="R5O56_RS29260"
                     /old_locus_tag="R5O56_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            5316..5867
                     /locus_tag="R5O56_RS29265"
                     /old_locus_tag="R5O56_29260"
     CDS             5316..5867
                     /locus_tag="R5O56_RS29265"
                     /old_locus_tag="R5O56_29260"
                     /inference="COORDINATES: protein motif:HMM:NF022971.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_317982578.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKLRYKSILAAIENPTQE"
                     /name="DUF3226 domain-containing protein"
     gene            6313..6555
                     /locus_tag="R5O56_RS29270"
                     /old_locus_tag="R5O56_29265"
     CDS             6313..6555
                     /locus_tag="R5O56_RS29270"
                     /old_locus_tag="R5O56_29265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(6901..7302)
                     /locus_tag="R5O56_RS29275"
                     /old_locus_tag="R5O56_29270"
     CDS             complement(6901..7302)
                     /locus_tag="R5O56_RS29275"
                     /old_locus_tag="R5O56_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7302..7493)
                     /locus_tag="R5O56_RS29280"
                     /old_locus_tag="R5O56_29275"
     CDS             complement(7302..7493)
                     /locus_tag="R5O56_RS29280"
                     /old_locus_tag="R5O56_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7909..8022
                     /locus_tag="R5O56_RS29285"
                     /old_locus_tag="R5O56_29280"
                     /pseudo=""
     CDS             7909..8022
                     /locus_tag="R5O56_RS29285"
                     /old_locus_tag="R5O56_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            8367..9482
                     /locus_tag="R5O56_RS29290"
                     /old_locus_tag="R5O56_29285"
     CDS             8367..9482
                     /locus_tag="R5O56_RS29290"
                     /old_locus_tag="R5O56_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            9482..10114
                     /locus_tag="R5O56_RS29295"
                     /old_locus_tag="R5O56_29290"
     CDS             9482..10114
                     /locus_tag="R5O56_RS29295"
                     /old_locus_tag="R5O56_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            10480..10722
                     /locus_tag="R5O56_RS29300"
                     /old_locus_tag="R5O56_29295"
     CDS             10480..10722
                     /locus_tag="R5O56_RS29300"
                     /old_locus_tag="R5O56_29295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(11068..11469)
                     /locus_tag="R5O56_RS29305"
                     /old_locus_tag="R5O56_29300"
     CDS             complement(11068..11469)
                     /locus_tag="R5O56_RS29305"
                     /old_locus_tag="R5O56_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(11469..11660)
                     /locus_tag="R5O56_RS29310"
                     /old_locus_tag="R5O56_29305"
     CDS             complement(11469..11660)
                     /locus_tag="R5O56_RS29310"
                     /old_locus_tag="R5O56_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            12076..12189
                     /locus_tag="R5O56_RS29315"
                     /old_locus_tag="R5O56_29310"
                     /pseudo=""
     CDS             12076..12189
                     /locus_tag="R5O56_RS29315"
                     /old_locus_tag="R5O56_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          3449..3856
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          7615..8022
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          11782..12189
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tggcatataa caaaaacacg aacagaggct atcatgataa aggaaataag tttaaagaac
       61 ttcaaatgct tcaatgagct atatctaaag caattaaaga ctttaaatat catagctggg
      121 aaaaacaact acgggaaaac atctatactg gatgctattt tttgttttta tgatgtaaaa
      181 aacccggctg ttttgttaaa cattcaagca ttcagaaaag aaatggcaga aataaacaaa
      241 aacaagccat tttgggttag ttactttcat gatatggaca catcacaaaa gatgtccata
      301 gtcattcgag atgaacgctc tgaagtaaca caaacatatg aaacagaaac aaatcagaga
      361 ttagaatcaa gtttgtctct aaacgttgac accgtactat ctaaccaaaa catacctcgt
      421 caaactatcg gttctcaggc cacagtaaac tctttaaaaa taaatgtgac cgagacaccc
      481 aatgcgaaaa acagggtttc caataagctt ttctctatgc aattgaaaga agatggcgta
      541 gaaataaaat ccgaaataaa aaacgaaagg gatgggatta attataaatt taagacagca
      601 acaataataa caacatccag aaagataaac aaagaggcaa caattacaaa tgtatctagc
      661 ctactaacac aaaaaaggaa aaaagacata ctagaaaact taaaaaaaat agatgacaga
      721 attgtagata tagcaatatc tgcgataggt aacaataagg aaatatattt agacattgga
      781 tttagtgagc taaatgaaat atcaatgctt ggcgagggta tcagtcgagc attatctttt
      841 atcagctcag tgctggtaca ggaaaactca attattctta ttgatgaaat cgagaacgga
      901 atacattact cagtaattaa agacataata aaatcattaa tttcatctgc aaaacaaaac
      961 aacaatcaaa tatttgcaac aactcatagt caagatgtga tacgagcaat aaacgaaata
     1021 gatagcaaaa atgaagatat cgcttatatt agactaggaa gagaaaagaa ctccctaaaa
     1081 ccaacagccg tacaattcaa tatggatgat ttctcctact cagttgagaa cgactgggag
     1141 gtaagataat gaaagacata aataatagca aaaagatcat tttcgttgag ggtgatgatg
     1201 attttcattt tctatgcaac ctacttgaaa tacaaggcat taccgatgtt tttgtcgaaa
     1261 aaataaatgg aaaatcaaaa ttaaaacaag cacttagagc attcaaaaac attgattctt
     1321 tcgatgaaaa agaaagtatt tttattatat tagatgctga cacatcattt tctgacacag
     1381 aaagatcaat aattgccaca ttgcgagaac tacaaatctc ctcccccagc tcgcataacg
     1441 agatagctat gaatggcaat acaaaaattt ctttctttat aatgccagga aaagacaagc
     1501 aaggtgccat tgaagatctg ataattgaac acgcaagcac tcgagcgtta tatgaataca
     1561 taatagaatt tttcgaaaaa attaaaaata ataaagatga catctcagcg agtgacaaag
     1621 agtacactta tccaacaaac gaaaaaaaag ctaaggtaca agtctatctt agctgcaata
     1681 gagaatccga ctcaagaata ggaatatcca tgaaaaatgg aataataaac acagcagaca
     1741 attgctttga tgaaataaaa gattttattt caaaaatata aatccaaatc aagccagccc
     1801 ttcacgggct ggcttatcac cagggtgtta ttaaacgccg gataacgtat agaaagcatc
     1861 atacacttta gttatcagca atctcaaacg tcctaaagac atcaccagat gccaaaacaa
     1921 tgattgtgca gatctaatgt aaaagaccca gcacaagatg ctcccttaag ttatattctc
     1981 tctattcact taaaacaatt taacataaaa tcataattgt acgcaccgct aaaacggact
     2041 caggacgttt ctgacgggaa accggtaaaa caggcaacaa accacccgaa aacccgccag
     2101 aaacgcgcag gctctgtttt tacggcatgc atgagtatgc gcgcaagtgc atagcgcgga
     2161 aattcacacc attcagccag gggaagtcct gtttccggcg ttcttctcct ccgaaaacgg
     2221 tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc cggaaaaccg
     2281 gttttcagca ccttcaggaa tcgggttcgt cgacggccgg caatcacgtt atggcaagtc
     2341 gccggacgac tcaccacacg attcaccggc gattcgaatc gtgaattgaa tcaagagtca
     2401 ccatgagcaa aaaacaccca aagtgatgaa ttgaatcaag agtcgattca tcataacaat
     2461 atgataataa agcgtgattc acatatgaat cgtgggcaat tcgtcagcga ttcgccatag
     2521 agtcgtccac gacttggatc tgcactttgt aacacatcac cgcacattta tttttcatta
     2581 acccattgtt gtaaataagg aatctgcaaa attttaattt tgcgtggggt gtgggtattt
     2641 ttagtggtga ggagcgttca gagatctgac gatttttggg aaggttcagg aactccctgg
     2701 gatggccgga gaggaaaact gaagcatgac ggggtcagga ttttttccag aatttccacc
     2761 atggcgccgg ggattcagtt aattttgcct ggtgattttc aagaaggagc atggcctgtt
     2821 tcaggctgtc aatgtgcgaa tccttggaca aaagtaaggt gttcaggtgt tcaatctcct
     2881 gtttcagatg acggatcacc tgttcactct ggtcagcaga ggctgtatca cgctgtccag
     2941 agacctgtcc agaatggtgt tcacccttcg gttgctcaaa ggggccaaag acacgtaaaa
     3001 gctctgaagt atctaccatt tttgcattgc gctcaccgct acaggtggac aggtcacctg
     3061 ctttaattaa cctgtgcagt gttgttctac tcttacccgt cagtcgggca gcctcactaa
     3121 tagacactct ggccatcagt taccatcctg taatgcatcc agtctcgagg acagtgtacg
     3181 gaaaagatgc tccgcatcat cgtggagttt ctcacaaagg tcggcctcga catcgagatc
     3241 aagctcgtta agcttctcca gcaggagcag agactgattc tggatgaatt tagccatgtt
     3301 cagcgctgtt ttctgttgtt tcgtcataag aggcctcaga gcgtcagtgg tcagtgtagg
     3361 gatctcctct tttccgcttc ctcgctcact cagtcgctac gctcctgccg ttcggctgcg
     3421 gcgagcggca ggagcatgcg tagaaacgct cgagaaaacc gagtaatcgc atggaggaaa
     3481 gaaggaaagg ccgcacagcg gccgtttttc cataggctcc gcccccctga cgagcatcac
     3541 ggaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
     3601 tttccccctg atggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     3661 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc
     3721 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact gctgcgccta
     3781 atccggtaac tatcgtcttg agtccaaccc ggaaagacac gacaaagcgc cactggcagc
     3841 agccactggt aactgattta gggagttagt cttgaagtta tacgccagtt aaagctaaac
     3901 taaacgaaca gttttggtga ctgcgctccc tttaagccag ttacctcggt tcatagagcg
     3961 ccaactctac tgaacctttc gaaaaaaccg cccgccaagg cggttttttt cgttcacagc
     4021 cggaagctta cggctgctgc cagataatct caagaagaaa gtaaaaaaaa tcgattaaaa
     4081 cacaagcaat caaagttaat ctttgacata aagtctagct aatcacaatg aaagctgtac
     4141 attttattaa ggtactaaca aatcgtatgg catataacaa aaacacgaac agaggctatc
     4201 atgataaagg aaataagttt aaagaacttc aaatgcttca atgagctata tctaaagcaa
     4261 ttaaagactt taaatatcat agctgggaaa aacaactacg ggaaaacatc tatactggat
     4321 gctatttttt gtttttatga tgtaaaaaac ccggctgttt tgttaaacat tcaagcattc
     4381 agaaaagaaa tggcagaaat aaacaaaaac aagccatttt gggttagtta ctttcatgat
     4441 atggacacat cacaaaagat gtccatagtc attcgagatg aacgctctga agtaacacaa
     4501 acatatgaaa cagaaacaaa tcagagatta gaatcaagtt tgtctctaaa cgttgacacc
     4561 gtactatcta accaaaacat acctcgtcaa actatcggtt ctcaggccac agtaaactct
     4621 ttaaaaataa atgtgaccga gacacccaat gcgaaaaaca gggtttccaa taagcttttc
     4681 tctatgcaat tgaaagaaga tggcgtagaa ataaaatccg aaataaaaaa cgaaagggat
     4741 gggattaatt ataaatttaa gacagcaaca ataataacaa catccagaaa gataaacaaa
     4801 gaggcaacaa ttacaaatgt atctagccta ctaacacaaa aaaggaaaaa agacatacta
     4861 gaaaacttaa aaaaaataga tgacagaatt gtagatatag caatatctgc gataggtaac
     4921 aataaggaaa tatatttaga cattggattt agtgagctaa atgaaatatc aatgcttggc
     4981 gagggtatca gtcgagcatt atcttttatc agctcagtgc tggtacagga aaactcaatt
     5041 attcttattg atgaaatcga gaacggaata cattactcag taattaaaga cataataaaa
     5101 tcattaattt catctgcaaa acaaaacaac aatcaaatat ttgcaacaac tcatagtcaa
     5161 gatgtgatac gagcaataaa cgaaatagat agcaaaaatg aagatatcgc ttatattaga
     5221 ctaggaagag aaaagaactc cctaaaacca acagccgtac aattcaatat ggatgatttc
     5281 tcctactcag ttgagaacga ctgggaggta agataatgaa agacataaat aatagcaaaa
     5341 agatcatttt cgttgagggt gatgatgatt ttcattttct atgcaaccta cttgaaatac
     5401 aaggcattac cgatgttttt gtcgaaaaaa taaatggaaa atcaaaatta aaacaagcac
     5461 ttagagcatt caaaaacatt gattctttcg atgaaaaaga aagtattttt attatattag
     5521 atgctgacac atcattttct gacacagaaa gatcaataat tgccacattg cgagaactac
     5581 aaatctcctc ccccagctcg cataacgaga tagctatgaa tggcaataca aaaatttctt
     5641 tctttataat gccaggaaaa gacaagcaag gtgccattga agatctgata attgaacacg
     5701 caagcactcg agcgttatat gaatacataa tagaattttt cgaaaaaatt aaaaataata
     5761 aagatgacat ctcagcgagt gacaaagagt acacttatcc aacaaacgaa aaaaagctaa
     5821 ggtacaagtc tatcttagct gcaatagaga atccgactca agaataggaa tatccatgaa
     5881 aaatggaata ataaacacag cagacaattg ctttgatgaa ataaaagatt ttatttcaaa
     5941 aatataaatc caaatcaagc cagcccttca cgggctggct tatcaccagg gtgttattaa
     6001 acgccggata acgtatagaa agcatcatac actttagtta tcagcaatct caaacgtcct
     6061 aaagacatca ccagatgcca aaacaatgat tgtgcagatc taatgtaaaa gacccagcac
     6121 aagatgctcc cttaagttat attctctcta ttcacttaaa acaatttaac ataaaatcat
     6181 aattgtacgc accgctaaaa cggactcagg acgtttctga cgggaaaccg gtaaaacagg
     6241 caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg gcatgcatga
     6301 gtatgcgcgc aagtgcatag cgcggaaatt cacaccattc agccagggga agtcctgttt
     6361 ccggcgttct tctcctccga aaacggtgaa cggggacgca atttattgcg tggccgtgaa
     6421 gcgttgccgg cgcagccgga aaaccggttt tcagcacctt caggaatcgg gttcgtcgac
     6481 ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc accggcgatt
     6541 cgaatcgtga attgaatcaa gagtcaccat gagcaaaaaa cacccaaagt gatgaattga
     6601 atcaagagtc gattcatcat aacaatatga taataaagcg tgattcacat atgaatcgtg
     6661 ggcaattcgt cagcgattcg ccatagagtc gtccacgact tggatctgca ctttgtaaca
     6721 catcaccgca catttatttt tcattaaccc attgttgtaa ataaggaatc tgcaaaattt
     6781 taattttgcg tggggtgtgg gtatttttag tggtgaggag cgttcagaga tctgacgatt
     6841 tttgggaagg ttcaggaact ccctgggatg gccggagagg aaaactgaag catgacgggg
     6901 tcaggatttt ttccagaatt tccaccatgg cgccggggat tcagttaatt ttgcctggtg
     6961 attttcaaga aggagcatgg cctgtttcag gctgtcaatg tgcgaatcct tggacaaaag
     7021 taaggtgttc aggtgttcaa tctcctgttt cagatgacgg atcacctgtt cactctggtc
     7081 agcagaggct gtatcacgct gtccagagac ctgtccagaa tggtgttcac ccttcggttg
     7141 ctcaaagggg ccaaagacac gtaaaagctc tgaagtatct accatttttg cattgcgctc
     7201 accgctacag gtggacaggt cacctgcttt aattaacctg tgcagtgttg ttctactctt
     7261 acccgtcagt cgggcagcct cactaataga cactctggcc atcagttacc atcctgtaat
     7321 gcatccagtc tcgaggacag tgtacggaaa agatgctccg catcatcgtg gagtttctca
     7381 caaaggtcgg cctcgacatc gagatcaagc tcgttaagct tctccagcag gagcagagac
     7441 tgattctgga tgaatttagc catgttcagc gctgttttct gttgtttcgt cataagaggc
     7501 ctcagagcgt cagtggtcag tgtagggatc tcctcttttc cgcttcctcg ctcactcagt
     7561 cgctacgctc ctgccgttcg gctgcggcga gcggcaggag catgcgtaga aacgctcgag
     7621 aaaaccgagt aatcgcatgg aggaaagaag gaaaggccgc acagcggccg tttttccata
     7681 ggctccgccc ccctgacgag catcacggaa tctgacgctc aaatcagtgg tggcgaaacc
     7741 cgacaggact ataaagatac caggcgtttc cccctgatgg ctccctcgtg cgctctcctg
     7801 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     7861 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     7921 cccgttcagt ccgactgctg cgcctaatcc ggtaactatc gtcttgagtc caacccggaa
     7981 agacacgaca aagcgccact ggcagcagcc actggtaact gatttaggga gttagtcttg
     8041 aagttatacg ccagttaaag ctaaactaaa cgaacagttt tggtgactgc gctcccttta
     8101 agccagttac ctcggttcat agagcgccaa ctctactgaa cctttcgaaa aaaccgcccg
     8161 ccaaggcggt ttttttcgtt cacagccgga agcttacggc tgctgccaga taatctcaag
     8221 aagaaagtaa aaaaaatcga ttaaaacaca agcaatcaaa gttaatcttt gacataaagt
     8281 ctagctaatc acaatgaaag ctgtacattt tattaaggta ctaacaaatc gtatggcata
     8341 taacaaaaac acgaacagag gctatcatga taaaggaaat aagtttaaag aacttcaaat
     8401 gcttcaatga gctatatcta aagcaattaa agactttaaa tatcatagct gggaaaaaca
     8461 actacgggaa aacatctata ctggatgcta ttttttgttt ttatgatgta aaaaacccgg
     8521 ctgttttgtt aaacattcaa gcattcagaa aagaaatggc agaaataaac aaaaacaagc
     8581 cattttgggt tagttacttt catgatatgg acacatcaca aaagatgtcc atagtcattc
     8641 gagatgaacg ctctgaagta acacaaacat atgaaacaga aacaaatcag agattagaat
     8701 caagtttgtc tctaaacgtt gacaccgtac tatctaacca aaacatacct cgtcaaacta
     8761 tcggttctca ggccacagta aactctttaa aaataaatgt gaccgagaca cccaatgcga
     8821 aaaacagggt ttccaataag cttttctcta tgcaattgaa agaagatggc gtagaaataa
     8881 aatccgaaat aaaaaacgaa agggatggga ttaattataa atttaagaca gcaacaataa
     8941 taacaacatc cagaaagata aacaaagagg caacaattac aaatgtatct agcctactaa
     9001 cacaaaaaag gaaaaaagac atactagaaa acttaaaaaa aatagatgac agaattgtag
     9061 atatagcaat atctgcgata ggtaacaata aggaaatata tttagacatt ggatttagtg
     9121 agctaaatga aatatcaatg cttggcgagg gtatcagtcg agcattatct tttatcagct
     9181 cagtgctggt acaggaaaac tcaattattc ttattgatga aatcgagaac ggaatacatt
     9241 actcagtaat taaagacata ataaaatcat taatttcatc tgcaaaacaa aacaacaatc
     9301 aaatatttgc aacaactcat agtcaagatg tgatacgagc aataaacgaa atagatagca
     9361 aaaatgaaga tatcgcttat attagactag gaagagaaaa gaactcccta aaaccaacag
     9421 ccgtacaatt caatatggat gatttctcct actcagttga gaacgactgg gaggtaagat
     9481 aatgaaagac ataaataata gcaaaaagat cattttcgtt gagggtgatg atgattttca
     9541 ttttctatgc aacctacttg aaatacaagg cattaccgat gtttttgtcg aaaaaataaa
     9601 tggaaaatca aaattaaaac aagcacttag agcattcaaa aacattgatt ctttcgatga
     9661 aaaagaaagt atttttatta tattagatgc tgacacatca ttttctgaca cagaaagatc
     9721 aataattgcc acattgcgag aactacaaat ctcctccccc agctcgcata acgagatagc
     9781 tatgaatggc aatacaaaaa tttctttctt tataatgcca ggaaaagaca agcaaggtgc
     9841 cattgaagat ctgataattg aacacgcaag cactcgagcg ttatatgaat acataataga
     9901 atttttcgaa aaaattaaaa ataataaaga tgacatctca gcgagtgaca aagagtacac
     9961 ttatccaaca aacgaaaaaa aagctaaggt acaagtctat cttagctgca atagagaatc
    10021 cgactcaaga ataggaatat ccatgaaaaa tggaataata aacacagcag acaattgctt
    10081 tgatgaaata aaagatttta tttcaaaaat ataaatccaa atcaagccag cccttcacgg
    10141 gctggcttat caccagggtg ttattaaacg ccggataacg tatagaaagc atcatacact
    10201 ttagttatca gcaatctcaa acgtcctaaa gacatcacca gatgccaaaa caatgattgt
    10261 gcagatctaa tgtaaaagac ccagcacaag atgctccctt aagttatatt ctctctattc
    10321 acttaaaaca atttaacata aaatcataat tgtacgcacc gctaaaacgg actcaggacg
    10381 tttctgacgg gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg
    10441 caggctctgt ttttacggca tgcatgagta tgcgcgcaag tgcatagcgc ggaaattcac
    10501 accattcagc caggggaagt cctgtttccg gcgttcttct cctccgaaaa cggtgaacgg
    10561 ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca
    10621 gcaccttcag gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa gtcgccggac
    10681 gactcaccac acgattcacc ggcgattcga atcgtgaatt gaatcaagag tcaccatgag
    10741 caaaaaacac ccaaagtgat gaattgaatc aagagtcgat tcatcataac aatatgataa
    10801 taaagcgtga ttcacatatg aatcgtgggc aattcgtcag cgattcgcca tagagtcgtc
    10861 cacgacttgg atctgcactt tgtaacacat caccgcacat ttatttttca ttaacccatt
    10921 gttgtaaata aggaatctgc aaaattttaa ttttgcgtgg ggtgtgggta tttttagtgg
    10981 tgaggagcgt tcagagatct gacgattttt gggaaggttc aggaactccc tgggatggcc
    11041 ggagaggaaa actgaagcat gacggggtca ggattttttc cagaatttcc accatggcgc
    11101 cggggattca gttaattttg cctggtgatt ttcaagaagg agcatggcct gtttcaggct
    11161 gtcaatgtgc gaatccttgg acaaaagtaa ggtgttcagg tgttcaatct cctgtttcag
    11221 atgacggatc acctgttcac tctggtcagc agaggctgta tcacgctgtc cagagacctg
    11281 tccagaatgg tgttcaccct tcggttgctc aaaggggcca aagacacgta aaagctctga
    11341 agtatctacc atttttgcat tgcgctcacc gctacaggtg gacaggtcac ctgctttaat
    11401 taacctgtgc agtgttgttc tactcttacc cgtcagtcgg gcagcctcac taatagacac
    11461 tctggccatc agttaccatc ctgtaatgca tccagtctcg aggacagtgt acggaaaaga
    11521 tgctccgcat catcgtggag tttctcacaa aggtcggcct cgacatcgag atcaagctcg
    11581 ttaagcttct ccagcaggag cagagactga ttctggatga atttagccat gttcagcgct
    11641 gttttctgtt gtttcgtcat aagaggcctc agagcgtcag tggtcagtgt agggatctcc
    11701 tcttttccgc ttcctcgctc actcagtcgc tacgctcctg ccgttcggct gcggcgagcg
    11761 gcaggagcat gcgtagaaac gctcgagaaa accgagtaat cgcatggagg aaagaaggaa
    11821 aggccgcaca gcggccgttt ttccataggc tccgcccccc tgacgagcat cacggaatct
    11881 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11941 ctgatggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
    12001 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
    12061 tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc ctaatccggt
    12121 aactatcgtc ttgagtccaa cccggaaaga cacgacaaag cgccactggc agcagccact
    12181 ggtaactgat ttagggagtt agtcttgaag ttatacgcca gttaaagcta aactaaacga
    12241 acagttttgg tgactgcgct ccctttaagc cagttacctc ggttcataga gcgccaactc
    12301 tactgaacct ttcgaaaaaa ccgcccgcca aggcggtttt tttcgttcac agccggaagc
    12361 ttacggctgc tgccagataa tctcaagaag aaagtaaaaa aaatcgatta aaacacaagc
    12421 aatcaaagtt aatctttgac ataaagtcta gctaatcaca atgaaagctg tacattttat
    12481 taaggtacta acaaatcgta
//
