LOCUS       NZ_CP137361             4167 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP97_L17_12.23_ST147_NDM5 plasmid
            unnamed3, complete sequence.
ACCESSION   NZ_CP137361
VERSION     NZ_CP137361.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37951970
            Assembly: GCF_033240085.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 10:55:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,848
            CDSs (total)                      :: 5,724
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 97 of 188
            Pseudo Genes (incomplete)         :: 95 of 188
            Pseudo Genes (internal stop)      :: 27 of 188
            Pseudo Genes (multiple problems)  :: 26 of 188
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137361.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP97_L17_12.23_ST147_NDM5"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-03"
                     /genotype="ST147"
     gene            complement(join(4063..4167,1..138))
                     /locus_tag="R5O58_RS29220"
                     /old_locus_tag="R5O58_29220"
     CDS             complement(join(4063..4167,1..138))
                     /locus_tag="R5O58_RS29220"
                     /old_locus_tag="R5O58_29220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(504..1136)
                     /locus_tag="R5O58_RS29225"
                     /old_locus_tag="R5O58_29225"
     CDS             complement(504..1136)
                     /locus_tag="R5O58_RS29225"
                     /old_locus_tag="R5O58_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1136..2251)
                     /locus_tag="R5O58_RS29230"
                     /old_locus_tag="R5O58_29230"
     CDS             complement(1136..2251)
                     /locus_tag="R5O58_RS29230"
                     /old_locus_tag="R5O58_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2596..2709)
                     /locus_tag="R5O58_RS29235"
                     /old_locus_tag="R5O58_29235"
                     /pseudo=""
     CDS             complement(2596..2709)
                     /locus_tag="R5O58_RS29235"
                     /old_locus_tag="R5O58_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3125..3316
                     /locus_tag="R5O58_RS29240"
                     /old_locus_tag="R5O58_29240"
     CDS             3125..3316
                     /locus_tag="R5O58_RS29240"
                     /old_locus_tag="R5O58_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3316..3717
                     /locus_tag="R5O58_RS29245"
                     /old_locus_tag="R5O58_29245"
     CDS             3316..3717
                     /locus_tag="R5O58_RS29245"
                     /old_locus_tag="R5O58_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2597..3004)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 aaaccggttt tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg
       61 ttcaccgttt tcggaggaga agaacgccgg aaacaggact tcccctggct gaatggtgtg
      121 aatttccgcg ctatgcactt gcgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc
      181 gtttctggcg ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgt
      241 cctgagtccg ttttagcggt gcgtacaatt atgattttat gttaaattgt tttaagtgaa
      301 tagagagaat ataacttaag ggagcatctt gtgctgggtc ttttacatta gatctgcaca
      361 atcattgttt tggcatctgg tgatgtcttt aggacgtttg agattgctga taactaaagt
      421 gtatgatgct ttctatacgt tatccggcgt ttaataacac cctggtgata agccagcccg
      481 tgaagggctg gcttgatttg gatttatatt tttgaaataa aatcttttat ttcatcaaag
      541 caattgtctg ctgtgtttat tattccattt ttcatggata ttcctattct tgagtcggat
      601 tctctattgc agctaagata gacttgtacc ttagcttttt tttcgtttgt tggataagtg
      661 tactctttgt cactcgctga gatgtcatct ttattatttt taattttttc gaaaaattct
      721 attatgtatt catataacgc tcgagtgctt gcgtgttcaa ttatcagatc ttcaatggca
      781 ccttgcttgt cttttcctgg cattataaag aaagaaattt ttgtattgcc attcatagct
      841 atctcgttat gcgagctggg ggaggagatt tgtagttctc gcaatgtggc aattattgat
      901 ctttctgtgt cagaaaatga tgtgtcagca tctaatataa taaaaatact ttctttttca
      961 tcgaaagaat caatgttttt gaatgctcta agtgcttgtt ttaattttga ttttccattt
     1021 attttttcga caaaaacatc ggtaatgcct tgtatttcaa gtaggttgca tagaaaatga
     1081 aaatcatcat caccctcaac gaaaatgatc tttttgctat tatttatgtc tttcattatc
     1141 ttacctccca gtcgttctca actgagtagg agaaatcatc catattgaat tgtacggctg
     1201 ttggttttag ggagttcttt tctcttccta gtctaatata agcgatatct tcatttttgc
     1261 tatctatttc gtttattgct cgtatcacat cttgactatg agttgttgca aatatttgat
     1321 tgttgttttg ttttgcagat gaaattaatg attttattat gtctttaatt actgagtaat
     1381 gtattccgtt ctcgatttca tcaataagaa taattgagtt ttcctgtacc agcactgagc
     1441 tgataaaaga taatgctcga ctgataccct cgccaagcat tgatatttca tttagctcac
     1501 taaatccaat gtctaaatat atttccttat tgttacctat cgcagatatt gctatatcta
     1561 caattctgtc atctattttt tttaagtttt ctagtatgtc ttttttcctt ttttgtgtta
     1621 gtaggctaga tacatttgta attgttgcct ctttgtttat ctttctggat gttgttatta
     1681 ttgttgctgt cttaaattta taattaatcc catccctttc gttttttatt tcggatttta
     1741 tttctacgcc atcttctttc aattgcatag agaaaagctt attggaaacc ctgtttttcg
     1801 cattgggtgt ctcggtcaca tttattttta aagagtttac tgtggcctga gaaccgatag
     1861 tttgacgagg tatgttttgg ttagatagta cggtgtcaac gtttagagac aaacttgatt
     1921 ctaatctctg atttgtttct gtttcatatg tttgtgttac ttcagagcgt tcatctcgaa
     1981 tgactatgga catcttttgt gatgtgtcca tatcatgaaa gtaactaacc caaaatggct
     2041 tgtttttgtt tatttctgcc atttcttttc tgaatgcttg aatgtttaac aaaacagccg
     2101 ggttttttac atcataaaaa caaaaaatag catccagtat agatgttttc ccgtagttgt
     2161 ttttcccagc tatgatattt aaagtcttta attgctttag atatagctca ttgaagcatt
     2221 tgaagttctt taaacttatt tcctttatca tgatagcctc tgttcgtgtt tttgttatat
     2281 gccatacgat ttgttagtac cttaataaaa tgtacagctt tcattgtgat tagctagact
     2341 ttatgtcaaa gattaacttt gattgcttgt gttttaatcg atttttttta ctttcttctt
     2401 gagattatct ggcagcagcc gtaagcttcc ggctgtgaac gaaaaaaacc gccttggcgg
     2461 gcggtttttt cgaaaggttc agtagagttg gcgctctatg aaccgaggta actggcttaa
     2521 agggagcgca gtcaccaaaa ctgttcgttt agtttagctt taactggcgt ataacttcaa
     2581 gactaactcc ctaaatcagt taccagtggc tgctgccagt ggcgctttgt cgtgtctttc
     2641 cgggttggac tcaagacgat agttaccgga ttaggcgcag cagtcggact gaacgggggg
     2701 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     2761 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     2821 gagagcgcac gagggagcca tcagggggaa acgcctggta tctttatagt cctgtcgggt
     2881 ttcgccacca ctgatttgag cgtcagattc cgtgatgctc gtcagggggg cggagcctat
     2941 ggaaaaacgg ccgctgtgcg gcctttcctt ctttcctcca tgcgattact cggttttctc
     3001 gagcgtttct acgcatgctc ctgccgctcg ccgcagccga acggcaggag cgtagcgact
     3061 gagtgagcga ggaagcggaa aagaggagat ccctacactg accactgacg ctctgaggcc
     3121 tcttatgacg aaacaacaga aaacagcgct gaacatggct aaattcatcc agaatcagtc
     3181 tctgctcctg ctggagaagc ttaacgagct tgatctcgat gtcgaggccg acctttgtga
     3241 gaaactccac gatgatgcgg agcatctttt ccgtacactg tcctcgagac tggatgcatt
     3301 acaggatggt aactgatggc cagagtgtct attagtgagg ctgcccgact gacgggtaag
     3361 agtagaacaa cactgcacag gttaattaaa gcaggtgacc tgtccacctg tagcggtgag
     3421 cgcaatgcaa aaatggtaga tacttcagag cttttacgtg tctttggccc ctttgagcaa
     3481 ccgaagggtg aacaccattc tggacaggtc tctggacagc gtgatacagc ctctgctgac
     3541 cagagtgaac aggtgatccg tcatctgaaa caggagattg aacacctgaa caccttactt
     3601 ttgtccaagg attcgcacat tgacagcctg aaacaggcca tgctccttct tgaaaatcac
     3661 caggcaaaat taactgaatc cccggcgcca tggtggaaat tctggaaaaa atcctgaccc
     3721 cgtcatgctt cagttttcct ctccggccat cccagggagt tcctgaacct tcccaaaaat
     3781 cgtcagatct ctgaacgctc ctcaccacta aaaataccca caccccacgc aaaattaaaa
     3841 ttttgcagat tccttattta caacaatggg ttaatgaaaa ataaatgtgc ggtgatgtgt
     3901 tacaaagtgc agatccaagt cgtggacgac tctatggcga atcgctgacg aattgcccac
     3961 gattcatatg tgaatcacgc tttattatca tattgttatg atgaatcgac tcttgattca
     4021 attcatcact ttgggtgttt tttgctcatg gtgactcttg attcaattca cgattcgaat
     4081 cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca taacgtgatt gccggccgtc
     4141 gacgaacccg attcctgaag gtgctga
//
