LOCUS       NZ_CP137350            11970 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain H10544 plasmid pH10544-4, complete
            sequence.
ACCESSION   NZ_CP137350
VERSION     NZ_CP137350.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37954868
            Assembly: GCF_033259165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Chen,L. and Du,H.
  TITLE     Complete sequence of strain H10544
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Chen,L. and Du,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) Department of Clinical Laboratory, The
            Second Affiliated Hospital of Soochow University, Xihuan, Suzhou
            215004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.49
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 13:09:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,944
            CDSs (total)                      :: 5,823
            Genes (coding)                    :: 5,643
            CDSs (with protein)               :: 5,643
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 67 of 180
            Pseudo Genes (incomplete)         :: 122 of 180
            Pseudo Genes (internal stop)      :: 24 of 180
            Pseudo Genes (multiple problems)  :: 33 of 180
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137350.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="H10544"
                     /isolation_source="Urinary tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pH10544-4"
                     /geo_loc_name="China: Suzhou"
                     /lat_lon="31.30 N 120.58 E"
                     /collection_date="2019"
                     /collected_by="a teaching hospital"
     gene            complement(join(10378..11970,1..93))
                     /locus_tag="R5H10_RS29655"
                     /old_locus_tag="R5H10_29655"
     CDS             complement(join(10378..11970,1..93))
                     /locus_tag="R5H10_RS29655"
                     /old_locus_tag="R5H10_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(198..1685)
                     /gene="ltrA"
                     /locus_tag="R5H10_RS29660"
                     /old_locus_tag="R5H10_29660"
     CDS             complement(198..1685)
                     /gene="ltrA"
                     /locus_tag="R5H10_RS29660"
                     /old_locus_tag="R5H10_29660"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            2330..2575
                     /locus_tag="R5H10_RS29665"
                     /old_locus_tag="R5H10_29665"
     CDS             2330..2575
                     /locus_tag="R5H10_RS29665"
                     /old_locus_tag="R5H10_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2849..3220
                     /gene="tolA"
                     /locus_tag="R5H10_RS29670"
                     /old_locus_tag="R5H10_29670"
     CDS             2849..3220
                     /gene="tolA"
                     /locus_tag="R5H10_RS29670"
                     /old_locus_tag="R5H10_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3217..3582
                     /locus_tag="R5H10_RS29675"
                     /old_locus_tag="R5H10_29675"
     CDS             3217..3582
                     /locus_tag="R5H10_RS29675"
                     /old_locus_tag="R5H10_29675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3757..4035
                     /locus_tag="R5H10_RS29680"
                     /old_locus_tag="R5H10_29680"
     CDS             3757..4035
                     /locus_tag="R5H10_RS29680"
                     /old_locus_tag="R5H10_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4023..4337
                     /locus_tag="R5H10_RS29685"
                     /old_locus_tag="R5H10_29685"
     CDS             4023..4337
                     /locus_tag="R5H10_RS29685"
                     /old_locus_tag="R5H10_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4501..4929
                     /locus_tag="R5H10_RS29690"
                     /old_locus_tag="R5H10_29690"
     CDS             4501..4929
                     /locus_tag="R5H10_RS29690"
                     /old_locus_tag="R5H10_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4955..5134
                     /locus_tag="R5H10_RS29695"
                     /old_locus_tag="R5H10_29695"
     CDS             4955..5134
                     /locus_tag="R5H10_RS29695"
                     /old_locus_tag="R5H10_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5161..5691)
                     /locus_tag="R5H10_RS29700"
                     /old_locus_tag="R5H10_29700"
     CDS             complement(5161..5691)
                     /locus_tag="R5H10_RS29700"
                     /old_locus_tag="R5H10_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5698..6429)
                     /gene="mobC"
                     /locus_tag="R5H10_RS29705"
                     /old_locus_tag="R5H10_29705"
     CDS             complement(5698..6429)
                     /gene="mobC"
                     /locus_tag="R5H10_RS29705"
                     /old_locus_tag="R5H10_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6429..8393)
                     /locus_tag="R5H10_RS29710"
                     /old_locus_tag="R5H10_29710"
     CDS             complement(6429..8393)
                     /locus_tag="R5H10_RS29710"
                     /old_locus_tag="R5H10_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9877..10026)
                     /locus_tag="R5H10_RS29715"
                     /old_locus_tag="R5H10_29715"
     CDS             complement(9877..10026)
                     /locus_tag="R5H10_RS29715"
                     /old_locus_tag="R5H10_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10111..10368)
                     /locus_tag="R5H10_RS29720"
                     /old_locus_tag="R5H10_29720"
     CDS             complement(10111..10368)
                     /locus_tag="R5H10_RS29720"
                     /old_locus_tag="R5H10_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             5378..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9196..9774
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5702..6429)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tttaccagac gtaccgttca cattcccact ggcctgacca ccattatgtc ccagacctga
       61 attacccgga cctcgaccgt ctccaccact cataccgttt tcctctttga catttaaaac
      121 aataaattaa agccaacatg tcgcggtagg gatacccgtt accggatacc ccccgcacag
      181 atcccggcgt gcgcgattta cgcaccgggc tcctgcctcg ggtgtctggc ggtgaaccgc
      241 tccacaggcc atggatgaag aacccgaacc cttggtagcc atgcggctgc cagtttgttt
      301 gctttcgtcc aggtcgtatc atccttctgg ctcctgcgcc tgagcgcccg gcgccagagg
      361 tttgttacgt gtgtcctgaa cttctgcatg gtggggaagt tgcccggtac cgagtgatag
      421 ttcaggtatc cctgaaccac tctcctgagc cattttccct gttcggggat tgagtaatgc
      481 cagcgccttc gcagaccgtc tttgatggct ttcagagttg ccgtcatccg atcccggcgg
      541 gtctttcgta tcagcatgaa cctgccgttg cgatctttcc cgctgatgtg cgtgaacccg
      601 aggaagttga acgtttctgg tttgcctttt cccctgatgg cacggttttc ggcagcgaag
      661 cggccgaact ccatcagacg ggttttctcc gggtgaaccg tgagtccgaa ctccctcagt
      721 ctgcgctgca tggctatacg gaagcgccgg gcatcgtatc gtttgtcgaa cccgatgacg
      781 atgtcatcgg cgtatctgac cattaccaca ttgcctgtgg catagcgacg tcgccactga
      841 tgcgcccaca gatcgaagac gtagtggagg tatatgtttg ccagcagcgg tgagatgacc
      901 gcaccctgtg gggtgccttc ctccgttgct cgccattgac cctcctccga cgtcccggct
      961 gtgagccact tacgtatgag cctgattacc ctccggtcgc cgatccgatg ctctgtgaac
     1021 ctgatcagcc attcgtggct caccctgtcg aagaactgac tgatgtcggc atccagtacc
     1081 cagtttacgt tagtgcgtac cagccctgtg gccagtgcgt ccagtgcatc gtgctggctt
     1141 cgcccgggtc tgaacccgta tgagaacccc ataaagtcgt tttcatagac tgcgttcagg
     1201 attttcacca gcgcatactg gacgatcttg tcctccagcg aggcgatgcc gagcgggcgt
     1261 tgttttccat ccgcttttgg gatgtagtga cgcctgccgg gctgcgccct gtagctgccc
     1321 tgatgtagcc tccggtgcag atctgttatg ttgttcttca tgtttccggc gtagtccatc
     1381 cacctgatgc catccactcc ggcggccgct ttcctgctca gggagaggaa tgcggcttcc
     1441 agtgcttcga ctgtcagcag gtggaacaat gctgtaaacc gttctttctt ccgctgcttc
     1501 gcagcttccc gcacgcgtga cagcctctgt gacatgcttt cccggctctg tgtccggcgc
     1561 atgtgtggct gttccgcgtt ccccttggcc ccgctccttc gctccactga ctccgctcct
     1621 ttcgggttgt tcgcctgctt cgccgctact atgagcgagt ccgacttctc ctctccgtac
     1681 atcaccggct atgactcctc gtcttcccgg tgcgggccat ctccgacact ggcagatggt
     1741 cagaggggag atctcccggt tcccgcgtag agatcgtatt gacatgccag ggtctcagac
     1801 cccgccgggt ccatgtggca ctcgcagtgt cgcaccctat gatgttgcct tccgttaaca
     1861 gtacaacgtc ggcacccggt aatttaatat acatttcgtg gctcaatggc tggcctgtca
     1921 acacccctgt caacgcttcg ccccatacct cgcggtatgc aacgcatgac tcggggacct
     1981 tgtggattgc tggtccttca atggtcgggg actttcaccc cttgatctct aaccggtctc
     2041 ccggcgcaca ctgtacatat acacagtata attattacct tactcctccc gttttcaggt
     2101 gtcaatatat gagattagtt ctcatcttca ggcacaaaaa agcgcccgga gccacctgta
     2161 agcagatcgc gtcttttccc gaaaatttct catggcaaca aattttatta tttaagacat
     2221 tcatcacact tacatttcag atggtataga cacatctgaa agctaactgg catgaggaga
     2281 ggacctgcgg ttaaactgtt cgggaaacct gagacgcccg gaggccacta tgacgcacct
     2341 gaaactgact cccgctgaag aaaagcgcct ggcggacgcc tttgtcgctc aggcactggc
     2401 catgggcctg aaagggaaca aaacgctgcc aaaggcggta aaacaacagg tgcgggaaag
     2461 ggtgcttcgt gccattcagg acgaacgtga agccaggcgg gcgcaggaaa tgcgtcggga
     2521 gaaatacgag gctacggaaa ccaccttcac ctggcagcca cagcgccgcc ggtaaggggt
     2581 atttaccata acgtcgcatt gcccgcaccg ctcagacggt ctgagggcgt ttccggcggc
     2641 tacgggataa aacaggcaac aaaccacccg gaaaaccgcc ggaaacgcgc ccggtaaatt
     2701 gtaacccgct gcatgcctat gcacgcaact gcatggcggg ccgtttggtg taaaaacgat
     2761 acgataagcc gtgtttatgt aaatgaacgt ggccgtaaag agtgacaaat atacatgtaa
     2821 cattttttac tgacaggtca tcaacccgat gcgtttaata ccctttatcc tgctggcatt
     2881 gtgcagcccc ttactcctga cggcctgtaa gcattccacc acccagccgc cgaaaattta
     2941 tactctggat aagtcatcta aaatcagtag ctacgctgcg gccatcaaga ccgctgttga
     3001 aatgcagatg tttgaaccag aggcctatgc gggaaaaatg tgcaccgtgc ggatatccat
     3061 acaacgtgat ggcactctga acagcgccat tgctgaagga ggagatccga aactctgtaa
     3121 agcagcaata tcagccgtta cccacgccaa aattccgccg gcaccggatg aaaatacctg
     3181 gcaaatattc aaaaacgccc ctctggattt cagaccatga aagcttttac tctgacagtg
     3241 ctggcggcca tgttcctcac tccggtacat gcgataccac taaaacccgt aaccacactg
     3301 cagccaacct acccggtaca ggccgcagca aaacgcatcg aaggtcaggt gaccgccacg
     3361 tttgatattc tggacgatgg acgagtggac aacgtgtcgc ttaccggctg tcaccttttt
     3421 tttcgggaaa cgctgacggc aatgaggcac tggctatatc ccgcagggca cccggcaatg
     3481 cgacaggtta taaattttga attcagtctg aaaaatgacg gcttacagga tccgcgacca
     3541 gtcaccaccc aaaagctcaa tgatgatggt tgccgccagt aacaggtcct ttaacccgca
     3601 ggctgcaata gtcctggatg atgagtgagc ctgtagcagc cctgattagt ggaagcgaag
     3661 ctgaaaaatg ggcagcaaaa gccgtatttc tggcaaatag cgccgccacg gattaaagta
     3721 gtcatgtgta atacatatga ctacaaaggg gcttttatgt catcagcaac atccatcagg
     3781 ctcgatgaag aacttaagga ccggctgaaa acgctggccg atgaccggca ccgctcagcg
     3841 cacgcgctaa tgctggaggc aatcaccgaa tacatcgacc gggaagagaa gcgcagccag
     3901 tacctgcggg acggacaggc cgcatggcag cactatcagg aaacgggtct gcaccttact
     3961 gcggaagaag cggaagcctg gataagtacc tggggaactg agaacgagca ggatgctccg
     4021 ccatgccaca ggtaacgatt tccgcgctgg cacagcgcga tttacaacgc cttcaggact
     4081 ttctcaaaac caaaaatcgg ctggcagcca gaaaaggagg tgaggtgatc gtccgggcta
     4141 tccagcaact gaaaacattg ccagacattg gccgcccggt gccgtttctg ccgctggaat
     4201 ataaggaact ggtgatcggg tttggcgaca gtggctatgt gatgctctac cgccacgacc
     4261 gggaaatgga ccagattgtg atcgtcaccg tcaggcatca gaaagaatcc gggtatccgg
     4321 gggcagacag cctctaataa ccgatgacgg gggcgcaatt cattgcgtgg ccgtcgccgg
     4381 tttgacaagc gcagcgcgtc agaaaaccac atcctgaacg gaaaaacggg taaaattcag
     4441 cgatacattt accgtgagaa attacacacc tttcacaccc gataaaatca ggggtataca
     4501 gtggcgaaaa cattgctcaa cctttcacag gcagcacaag ccgcgggcat taccagaaga
     4561 acgctataca atcatgtaaa acagggaaag gttaccgtgt cacgtgacgg gaaaaacaac
     4621 cctgtcgtgg atgtgtcaga actgatccgt gtatacggaa atgtgaatat ccctgagaaa
     4681 caaattcccg gcatttcaca ccgtgaaaat acacaaaaaa atttcccaca ggaacagctt
     4741 ctggcaatgc aaaaggaact ggccgatctc cggcaggctg tcaccctgat gctggaagat
     4801 aaaacatccc gggaagaaga acgccgccag cacgatgatg agcgccggaa actccaggcc
     4861 gaagtggaca gattgacaac agaactgaca cagaagaaaa aacgcttctg gtccggctgg
     4921 tttagctgag aacttaccga agggaatgga gaacatgacg aagcaggaaa aaactgccct
     4981 caatatggct aaattcatta aggatcagtc gctgctgttg ctggaaaagc tgaacgagct
     5041 ggacatggat accccggccg acatgtgcga acggcttcac gaggatgcgg aaacactctg
     5101 gcaggtgatg cgggataagc tcggggaaga ataacagcac cggccatgcc ggtgctggcg
     5161 ttattttttg ccgaacggcc accaccggcg ggcgggtttg tcctccggca gttcaggcac
     5221 cggcacgtta aaatagcggc tgttatattc ccgcatcagc tccgctttca gcttctcgtt
     5281 caccgcgctt tccccgtgcc gctcgctgac cacctcaatc atcgcgttaa tctgctccgg
     5341 tgagcacttc ccggcatcaa ccacctgcac accgttctcg atggcgaacc gctccacctt
     5401 tcgatagagg taatcacaca gcttcattcc tgtgggtttg tcccgttccc attgcggatt
     5461 ttcaccggtg aaaaagtacg tggctttttc gtcgctgttg agccgctcgt catacatccc
     5521 gatggatttc atcatcgcca gatgctgggc ccggtcgaca aaacagaaaa catacgcccc
     5581 ggtctgccag gcgagcacca ccatgtcttc cagatgctcg ctggttggca taatgcagcg
     5641 ctcgccgtct ttgtcttcca cgtcctcaat aaataccatg tacagcgcca taatccctca
     5701 ctccgttgtc ataaatttaa agcacgcgaa cagcctctcc cggtccagct ccaccgtttt
     5761 gcgggcctcg ccggtctgtg gattcacgat ttcatccacc gccagaatcg cccgctgtac
     5821 ccggacaagc gtcggccggt cagggcagat gtaccagatt tcatcgatgc cgttcgcccg
     5881 gcggttgaac agatacccgg acaccacttc ctgataccgc ctgcggctct tgacggtgcg
     5941 ctctacctca aatgccaccg ttctgccgtc cagctccgcc agcgcgtccg gcactttcat
     6001 ccctttcagg gccatacggt gcaggtaccg ccagccggtg gccccccggc cctccatgga
     6061 gaggcgcagg gactgcacgg ccagccggtg cggcagctgc gccgccgaca cccttgacgg
     6121 ctgaaacgga ataacgtccg tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg
     6181 cggcgtcagc ccccacacgc tgacctgacg gccgtcaacc gggtatttcg cccggaccag
     6241 cagccctttc gccaccagac tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc
     6301 cagccgtcgc gcaaccagct ccgccgtgga acacagctct tcccgcagcc agcgcagggt
     6361 cagccgtgtt ttctccgcgt tccgcgcctg ccgcttcgcg tggctgacgt tgtactgctc
     6421 cagtgtcatc agtaaagccc cgcaaaatca tcatccgctt ttggtccgcc gccgtcggcc
     6481 ggaggcaggt actccgccag cgggtccgtt ttcgccagca ccgggaaggt tttcagcgaa
     6541 atttcccggc gctcaacccg cagcgggctg ctgaacgcca gccgcgccac gcccagcccg
     6601 gtcatcaccg cgaacccgtc aggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc
     6661 gcctccttct ggatgaagct gtcgccgctg atgtgctcca cgttccccgc ctcccggctg
     6721 gcccggcggc gctccacgtc cacccggatc tccccggtct ggcccgccgc ccactgcgcg
     6781 gactcctggc tggccgcgcg atagaaccag cggatcggcg tgttatccag taccgtggtt
     6841 ttcacaaaat cagcgggcag atcctgcccg cactgaccga agtcgcccag cgactggtgc
     6901 gcgagcagca ggttgcagtc ccggctgcgc agcgtcccca gcgcgttcag cacgtacttc
     6961 gacagcagat atttaatctc atcgagcatg atgctcgcgt gcggccacct ccggaactca
     7021 tcccgcgcaa tgatgatttg cgcgcagcgg gcaaacagca tcttctgcac ccggatcacc
     7081 gcctcatcgt ccatcgagcc gatgacgtac aggcagccgc cgccgttgat tatccccgcc
     7141 acatcgatac cgctgtccgt ctgcaggacg ctcaggtcgg ccacgttctc cagcttggtg
     7201 atcagcccct tcacgtcctt tttcagggcc tccggcaacg cgtacgccgc cgccagcacc
     7261 tgctgaatgt ttgccccctg cgggaactgc tccgcaatca gcttcgccgc tttctgctcg
     7321 ctgatgcggt agtggtccgc cacgtcgccg gtctcactca ggttaaaccc ggcaaccagc
     7381 agattcttca gggcgtactg gtcgagatcg cggaacagat tcagctgcgc cggtttcccc
     7441 ggccgcagat ccagcagggt gaagtccggg cagtgcgccc ggaagacgtg cggcgcccac
     7501 gcgtcccctt tggggtcgaa gtacaccaca gcgtccccga aatcgcgtac gcagcgaatg
     7561 agcgcgttgg tggccatcgc gctcttgccg ctcccgggca ggccgaggat ctgaatattg
     7621 gtcttgcgcc aggtatcacg agggaccagc accggctggc cctgctcatc cttacccaga
     7681 aacaccgcct gctgtttccg ggcctgctcc gtggaaaaat cggccagcgc gttaaccggt
     7741 accggctccc cgaaatgcag gtggcggacg tctgtcattt ctccggtccg ctggctgctg
     7801 cgccggctct cctgattcag tttcgcgttc accgcctcgt cagcggccgc aaaatgacca
     7861 tcaagcagca ggaacgccag cagccgcgcc agaaggacgc cggcgacaag ccctccggca
     7921 caccaggcta tcccgaacag gttagccggc tgcagcaggt aataatctgc gttcgcatag
     7981 aggtccgggc gcaggacggt ttctccccag ctctcgataa acacggcgac aaccagcccc
     8041 cccagaaggg ccagaccggc agcgccggtc agccagtacg cgtagttcca gacatgtcgc
     8101 cgtgccctga ccatccgccc gggtgaatcc agcgaggcca gcaccgaaaa tggcacagtc
     8161 cggttattat cgcgcttata gcgatcggca tactcccgcc gcgccttatg gctcagcccc
     8221 acaaaacgca ttcggccggc ccagagttca gccagaccga aaaacaggcc gataaccaga
     8281 aaaaaaccgg ccagactgta atactcccgg tacagccagc tgacccacgg gtaaatctgc
     8341 cgggccagct ccgggatatg ccactgccag gcaagcgaat ccgtattaaa catgcaatct
     8401 ctctccttca gacgacgggt ttaccggcgt gatgcgcagc ccccgctgca caagccgcgg
     8461 acatgcccca aggcatggcg gcggcggtca cgtccgtggt gattgcgttc agctttcacc
     8521 gcggaggcca gcctctttcc gccccatgcg gaaaaggcgc agccccggag gaacaacgag
     8581 cacgccccag cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc cggtgttccg
     8641 gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt
     8701 gctgcttcgt caggtgcgtc cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg
     8761 acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc cggacagagc cagaaagagg
     8821 ggaaaagacc gtgcgtcggt ggcgtccagc cagcgtaagc aaggtctttt cccctgaagt
     8881 cagccacgcc ccagcgggga tgacttgcat ttctccgcaa ggagaaaaaa gcaacagaga
     8941 taggttaacg taaaatgttc agcgaaaaac cgacatggtt cacctatcct gataattgat
     9001 cgtcagggaa tagaaagact taatcagggg acaatatccc acatcagcgc gtccggtgac
     9061 cgtcttgcag gtgccgggga aattgcgcct cctcgctcac tcggtcgcta cgctccgggc
     9121 gtgagactgc ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag
     9181 caggggacta acatgtgagg caaaacggca gggccgcgcc ggtggcgttt ttccataggc
     9241 tccgccctcc tgccagagtt cacataaaca gacgcttttt cggtgcatct gtgggagccg
     9301 tgaggctcaa ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca
     9361 ccgtttccgg taggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga
     9421 tacctgtccg ccgttctccc ttgcgggaag tgtggcgctt tctcatagct cacacactgg
     9481 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga actccccgtt
     9541 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc gggaaagcac
     9601 gcaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct
     9661 aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt
     9721 ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac
     9781 cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca
     9841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag
     9901 ttcagaggag gacgatggtg ccaccgttcc gccctgaaca tcccggatat aatttgcctg
     9961 acatgcggcc agaagaaagc cagcaacaat caatataaaa agaaaaatag tctttacctt
    10021 tttcatatac tttccctgta tttaaagact ccggcattca tccctgaaca ccggaagtca
    10081 gtatttcaaa tcatccttga taattttttg ttaccagtta ccgtcccgat aatcaaaagc
    10141 tatataacaa ttatccacat caggttttat ttgattttta aaaaatggct gtagtaaagg
    10201 aacccattct ttcttcaaat caaactcacc attattaata ttatccttta aaggcattcc
    10261 caagctttca atgacagaac catcgtcacc aaagtcttta gaatactctt cccctttaaa
    10321 ctcttcagtt ttcttatcaa accatgtcaa acgtaattta agccccatat ttacctctca
    10381 aagatatttt ttaatgttcc gttttggatc tggcccttta acctgcttac ccgttttggg
    10441 atcaaatgcg cctaggtgtt cgccatcact ggcacgatac ccttcaagct caccgtgctg
    10501 ggaatcccat tcataaattt tacgtttttt atcgccatac catcgggccc gcttaccacc
    10561 accaccttgt ttaggtgttt taggatcacc tcgctttaac tctccaagtc cctttatgtc
    10621 ttctgtttta ggtactggat gataatcatg gccgtagtct ttagtcccct tgcgaggctt
    10681 gttcttttcc tcatttaact tattttctgc atccttcgct tttttctcct tttgtttccg
    10741 gctctccatc gccccggtaa gtgcagcctt atctctggat aaggattcat tcattgcgtt
    10801 taaagatgcc tgagccgctt ttaatttctc attctgattc tgaatatcct tatcaagata
    10861 gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag tcacaaaatc
    10921 atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag cagcccttgc
    10981 accggggagc gccttctctg tcgatgcaat gcggctgttt aaggtcgcaa tatttttatc
    11041 ttcggcatcc agctcagctt tcgctttatc atagtctctt ttcagcccct cttccggatg
    11101 agcggcgtcc catgcctgct ggcgacgctt ttcttcctcc tgacgctgtt ttacctgtgc
    11161 cggggtaaga acatctgtca ccgacacata aaccggcttc tgtccggtct ctttcgggaa
    11221 acgaataacg gcctcacggg agttaccacc ggctgtaaaa ccagccggac gtgaatcacc
    11281 tttttcagca acaatgcctt ttggcggggc tttcgctgtc ggaacacctt tcggtacgct
    11341 cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac gttttgtcgg
    11401 tttcgcatcc accacaggga cactcattgg ccgacctgcg acaaccgcaa tatgctgccg
    11461 ctcatccttc accacatcca caacccgttg tctgacacga accgtcgcct ggtccagtgg
    11521 taaagtactt gccggagtct ccgtcaccgc atcggccggt aatgacgtca taatttttga
    11581 catcattttc ggatcatctt ttgcaatctc agaaggcagc acaccgtaga tcgcaatccc
    11641 ccacagacca aacttaaacg gccccttcag ggcagccagc acatcagcaa cggcggccga
    11701 caacgcatcg ccggaaactg ataaagccag tccttcagcc cccggagtag ccagggcagg
    11761 cagaccgaag gccatcgcag tggccgaaga ctgtccgcca ccagagctac cgctatgatt
    11821 gccgctgtta ccaccattac caccgggttt actcccgccg ttaccaaact cccccggatt
    11881 atagttatga atatctccat tcggcgtatg cgtcgtaccc cagcccggcc cgctgtttgg
    11941 atccgtacca ccacctgatg aagggccacc
//
