LOCUS       NZ_CP137344             5595 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain H8451 plasmid pH8451-4, complete
            sequence.
ACCESSION   NZ_CP137344
VERSION     NZ_CP137344.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37954806
            Assembly: GCF_033258285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Chen,L. and Du,H.
  TITLE     Complete sequence of strain H8451
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Chen,L. and Du,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) Department of Clinical Laboratory, The
            Second Affiliated Hospital of Soochow University, Xihuan, Suzhou
            215004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.49
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:39:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,844
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,569
            CDSs (with protein)               :: 5,569
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 59 of 153
            Pseudo Genes (incomplete)         :: 98 of 153
            Pseudo Genes (internal stop)      :: 18 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137344.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="H8451"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pH8451-4"
                     /geo_loc_name="China: Beijing"
                     /lat_lon="40.03 N 116.42 E"
                     /collection_date="2020"
                     /collected_by="a teaching hospital"
     gene            740..916
                     /locus_tag="R5H09_RS29175"
                     /old_locus_tag="R5H09_29175"
     CDS             740..916
                     /locus_tag="R5H09_RS29175"
                     /old_locus_tag="R5H09_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            906..1250
                     /locus_tag="R5H09_RS29180"
                     /old_locus_tag="R5H09_29180"
     CDS             906..1250
                     /locus_tag="R5H09_RS29180"
                     /old_locus_tag="R5H09_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1459..>1893
                     /locus_tag="R5H09_RS29185"
                     /old_locus_tag="R5H09_29185"
                     /pseudo=""
     CDS             1459..>1893
                     /locus_tag="R5H09_RS29185"
                     /old_locus_tag="R5H09_29185"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1928..2437
                     /locus_tag="R5H09_RS29190"
                     /old_locus_tag="R5H09_29190"
     CDS             1928..2437
                     /locus_tag="R5H09_RS29190"
                     /old_locus_tag="R5H09_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2444..2656
                     /locus_tag="R5H09_RS29195"
                     /old_locus_tag="R5H09_29195"
     CDS             2444..2656
                     /locus_tag="R5H09_RS29195"
                     /old_locus_tag="R5H09_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3323..3547
                     /locus_tag="R5H09_RS29200"
                     /old_locus_tag="R5H09_29200"
     CDS             3323..3547
                     /locus_tag="R5H09_RS29200"
                     /old_locus_tag="R5H09_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3531..3797
                     /locus_tag="R5H09_RS29205"
                     /old_locus_tag="R5H09_29205"
     CDS             3531..3797
                     /locus_tag="R5H09_RS29205"
                     /old_locus_tag="R5H09_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3867..4217)
                     /locus_tag="R5H09_RS29210"
                     /old_locus_tag="R5H09_29210"
     CDS             complement(3867..4217)
                     /locus_tag="R5H09_RS29210"
                     /old_locus_tag="R5H09_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4283..4828)
                     /locus_tag="R5H09_RS29215"
                     /old_locus_tag="R5H09_29215"
     CDS             complement(4283..4828)
                     /locus_tag="R5H09_RS29215"
                     /old_locus_tag="R5H09_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4854..5180)
                     /locus_tag="R5H09_RS29220"
                     /old_locus_tag="R5H09_29220"
     CDS             complement(4854..5180)
                     /locus_tag="R5H09_RS29220"
                     /old_locus_tag="R5H09_29220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          575..654
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2..389)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.258"
                     /coverage="89.0"
                     /evalue="1.1e-133"
     Typing          1241..2772
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgctgccag tggcgttttt gcgtgtccgt ccgggttgga ctcaagatga tagttaccgg
       61 aaagggcgca gcagtcgggc tgaacggggg gttcgtgcat acagcccagc ttggagcgaa
      121 cgacctaaac cgagccgaga taccagcagc gtgagctatg agaaagcgcc acgcttcccg
      181 aaagggaaaa aggcggaaca ggtatccggt aaacggcagg gtcggaacag gagagcgcac
      241 gagggagccg ccggagagaa atcaccggca tctttaagtc ctgtcgggtt tcgtcctcct
      301 ctgacttgag cgtccatttc tgtgatgctc gtcaggggag gcggagccta tggaaaaacg
      361 gcaacgccgc atccttttct ccggcccttt ctgatttgag cgcctttgct ttatcccgtt
      421 atccgtgagt cggtcacgcc cgccgcagcc gaacggcagg agcgcagcta ccgagtgagc
      481 gaggaggcgt catttcatct gtccgtgcat ggtattgcgg acgctcctga gccacatatc
      541 acacctgcca catgaggcac tttcatgaat cgtgctctca tgccaacata gtaagccagt
      601 atacacagcg taaacgctgt gactggttca gggctgcgcc ccgaaacccg ctaacaccac
      661 tgacgcgctg gcgcttgtcc aacgccggag cgacccggaa aacttttccg tgccgtccgg
      721 cgtttgcgaa ggagggataa tgcagaagcc gaagaagaag ccgctcgtcg taaaaaaaag
      781 atcctggcat ccggcagtga tggcaaatct gatgcgtcag gcacaggagg aagattaccg
      841 ccgggagcag atggcagagc ttgagcgttc agcccggtca cattttgcgg atatcggagg
      901 agaggatggc ggttaagcgt acccgttttt taggtatccg ggtgaccgat ggggagtacc
      961 agcagctgct ggagcgctgc aatggaaggc aactggcggt gtggatgcgg gaaacctgtc
     1021 tcgatacgcg cccggcgcgg tcgttgcggc tgccgtccat cgatccggtt ttgctgcgcc
     1081 agcttgccgg gatggggaac aacctcaacc agatcgcccg gaaaattaac ggcggccagt
     1141 ggtcaggcgc tgaccgggtt caggtggtgg ccgcgctgat ggccattgat gccgaactcg
     1201 agcggctgcg gcatgccgtg ctggaaaagg gggcagacga tgatcgttaa gtttcatccg
     1261 cgagggcgag gcggcggtgc cgggccggtg gattatctgc tggggaaaga ccgccagcgc
     1321 gacggggcca tcgtcctgca gggaaagccg gaggaggtcc gggagcttat cgacgcctcg
     1381 ccctacgcca aaaagtacac ctccggcgtc ctgtcttttg ctgaacagga tttaccgccc
     1441 ggccagcgtg aaaagctgat ggcgagcttc gagcgggttc tgatgcccgg actcgataaa
     1501 gaccagtaca gcgtgctgtg ggttgaacac cgggacaagg ggcggctgga actgaatttt
     1561 ctggtaccga acacggaact gctgaccggc aggcgtctcc agccgtacta cgaccgggca
     1621 gaccgtccgc gcatcgatgc gtggcagacc gtggtgaacg ggcgactggg gctgcacgac
     1681 ccgaacgcac cggagaaccg gcgcgcgctg gtgacgccat cttcgttacc caaaacgaag
     1741 caggaagccg cagaggctat tacgcggggc ttactggccc tcgcctcgtc aggggagctt
     1801 aaaacgcgtc aggacgtcac tgaggcgctg gaaagcgcag gttttgaggt cgtgcgcacc
     1861 acaaagagca gcatcagcat tgccgacccg gacggagggc gaaacatccg acttaaggga
     1921 gccatctatg aacagtcttt taacgctggc gaaggactta gagcagaaat cgaaagcgca
     1981 gcagcagagt accggcgaaa tgctgaaagc cgcattcagc gagcacgaga agtctgtcag
     2041 agcggaactg agcgaaagcg ggaagagaat cagcgccgcc atccgcgccc acgagcaggg
     2101 tatgagcgcg gccatgcagt cgaaccgcct gagcgtgatg cgtatggtcg ggcggacatg
     2161 gctgaccatc acgatggtgt caggactgct gttcgccagc ctgagcgggg tgttgtggta
     2221 tcaggggagc ttgatagcgt caaatctggc cgaaatcgac cggcagaacg cagcgctgtc
     2281 gaagctgaac gcgaagacct ggggcgtgac gtacctggaa gacagcaacg gacgttttct
     2341 ggtgctgccg aaggggacgg ccgtagacag gacgcagagc tggacggtcg ggaacgggcg
     2401 gtcgaagcag aacgcgctga gactggtgaa ggagtaagtg ccgatgaccg agctggaaaa
     2461 acagttgctg agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg
     2521 ggagagcgcc ttcggggaat ggcggaaaat gtcagggctt atgcaacggg agaacgcggc
     2581 gctgagcgag cgcgtgacgc gcttgagtca gcaggtggaa cgcttgagcg ggcaactgca
     2641 gcgtttgagc cggtagtgca gcgtcatgag atggccgtgg ccgcagaacg ggcgcacgag
     2701 caacggcagc acgaaaagga actgacggaa gcgcgttccc ggcagcgaag ctatgacggt
     2761 ccgtcgctgg gctgaaatgc aggaaaacag ggttctcatc gggcatttac gctgaaaccg
     2821 gatgtcattc acctggctgc atggctatgc agccaggtaa aatttatctg tcgcgaacgg
     2881 ggcggtttat cgggtggttt gttgccggtt ttacctgact gccaccccgt tcgcggcgaa
     2941 cccgtccggg gcggtgcggg caacgggtgt tatgtttaat cagcgtgaac ggcagataca
     3001 aaaaagcccc ggagggcttt taactactga cttatttttt tgaagatttg ctgggtttca
     3061 atttgtcagt atttcccggc gtttttttat tgtcacgctg gcgtttatca ggttcccctg
     3121 ttgaatcggc aattcgtttc ggacccggct taagacccat aatcgttatc ctttcaatag
     3181 ttaagaggaa agccctcaag ctagatatat tgaccgcgtt acggattatc aagaggggct
     3241 aacaagatta ttttttgttc ttttaggttg attgtggtac tgttgatata catgtatagc
     3301 aaatggtgtt cgggaggttt atatgcttgc tatccggtta tctgatgaga ttgagtcccg
     3361 tctggactcg ctggcgaagc aaaccggcag aacaaagacg ttttatgcgc gggaagcaat
     3421 actggcgcat ctggaagacc tggaggatta ttatctttca gcagaaactg ctgcacgcgt
     3481 tcgccgtggt gatgaagcag tgcattcgtc tgaagacgtg aggaagtcac ttggtctgga
     3541 cgattaacta ttccgatcgg gcgctcaaat cgttacgcaa gatggacaaa cagaacgcac
     3601 gacggattgt ggattttatg agtttacgca ttgcagttgc tgccgatcct cgccagtcag
     3661 ggaagccgct caaaggtgag ctgggcgagt tctggcgcta tcgggtggga gattatcgcg
     3721 ttctgtgtga gatccgagat gacgagcttg ttatccttgc cgccacgatt ggacatcgcc
     3781 gcgaagttta cgactgaagc ccgctatgcg ggcttttttg atgccgacaa caggtattat
     3841 ggtaaatact cgactaacta cgtgttctac tgtttgtcgg ttttagttaa ccagcaggtt
     3901 gtcttaacga taatgtagcc gttattttta actaataagg tcatattgtt aacgtcaggc
     3961 ataataaaag acattatggc ctcagagtta tcatcagaag cagctctctg cattactact
     4021 tcgattccct tttctttgaa tctataagta tccagttgcc tattaaactt agaaagacct
     4081 acaaccatac ttagtttgtt atctttaaca gtaagagatg cttcctgagt accattacat
     4141 cggtagtaag cggtgtaacc gtcagcaaaa gatcgaccgg ggaatataaa tgctaggaac
     4201 gcaattaacc aatacatagt tgagaatctt ggtattacac atttcactat cctctctttc
     4261 atttttcgtt caccatactt tgttatctga tttttaattt ttctatttta taaaccaagt
     4321 ctctaccaaa aaggcgaaaa ttatcctcaa ataataataa ttgtttaaga acatccatga
     4381 aagcagctgc ttcaccctca gttatgggag ctactctata taacatataa cctgagttaa
     4441 gggtttcttt tatactttct tcaatgctca ttataaatat tagtggatta tcatttttct
     4501 tcccccatgg gtttctaatt aatccttcga tgtactcagg tcctttgcgt cctattgcct
     4561 gcgcaatatt ttttatttca tctgcgtgag ggctttgttg tacagctttg tttatgcctg
     4621 cttgagtgat tttcagagac tctacaccgt gtatgttgta tgagataaaa tgtaataaat
     4681 taagatgcaa tgcaattaat ccatttttgt ttacatctga gtatttggat aaaaaacgta
     4741 atgaagtgtt aactctccct tgaagcctaa caattgggag tgctgccttg tatatttcct
     4801 tctccgttaa ataccgttta aaaaacataa ttctccgcct ccgtatagcg tgcctatttt
     4861 atttcgtcgt aaaacttagc tctgaaatat tcctttagaa ccatcatgtc attaataatt
     4921 gaatctatct catccatttt ttcttggtat tcttttaatg gtgaagggag cggtaactca
     4981 atatttgaaa agtgattggc gagataggga tcaaggtttc tcatccattc aggcttttcg
     5041 ccaatgcgtt cttcaactga aagtgaacct gataatctct ggttaataag atcacttaga
     5101 ctcattttgg ttttattcga aagccgttgt aaggattgct ttaatcctat atctagcatg
     5161 atatttgttc tttctctcat tacccccctt gctattgcgt tttttgttgc gcacaaagct
     5221 accattgcgc attaagaaac gcaataaaaa aggagggagg gagtggtttg tacatgatgt
     5281 gcagtgcgct gagggcatca gtggcgggtg tttttccgtg ggatggatgg agctccgggg
     5341 ataacagaac tgacgatcat ttaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5401 ctcttgcact gtaaacgaaa aaaccgcctg gggaggcggt ttgatcgaag gttaagtcag
     5461 ttggggaact gcttaaccgg gtaactgggt atacgagacc gcagatacca aaactgttct
     5521 ttcagtgtag ccgcagttag gcacccactt caagacctct caatatctct ctcgtatata
     5581 tccagttacc aatgg
//
