LOCUS       NZ_CP137343            10060 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Klebsiella pneumoniae strain H8451 plasmid pH8451-3, complete
            sequence.
ACCESSION   NZ_CP137343
VERSION     NZ_CP137343.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37954806
            Assembly: GCF_033258285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Chen,L. and Du,H.
  TITLE     Complete sequence of strain H8451
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Chen,L. and Du,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) Department of Clinical Laboratory, The
            Second Affiliated Hospital of Soochow University, Xihuan, Suzhou
            215004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.49
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/03/2023 12:39:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,844
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,569
            CDSs (with protein)               :: 5,569
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 59 of 153
            Pseudo Genes (incomplete)         :: 98 of 153
            Pseudo Genes (internal stop)      :: 18 of 153
            Pseudo Genes (multiple problems)  :: 22 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137343.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="H8451"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pH8451-3"
                     /geo_loc_name="China: Beijing"
                     /lat_lon="40.03 N 116.42 E"
                     /collection_date="2020"
                     /collected_by="a teaching hospital"
     gene            58..486
                     /locus_tag="R5H09_RS29110"
                     /old_locus_tag="R5H09_29110"
     CDS             58..486
                     /locus_tag="R5H09_RS29110"
                     /old_locus_tag="R5H09_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            512..691
                     /locus_tag="R5H09_RS29115"
                     /old_locus_tag="R5H09_29115"
     CDS             512..691
                     /locus_tag="R5H09_RS29115"
                     /old_locus_tag="R5H09_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(718..1248)
                     /locus_tag="R5H09_RS29120"
                     /old_locus_tag="R5H09_29120"
     CDS             complement(718..1248)
                     /locus_tag="R5H09_RS29120"
                     /old_locus_tag="R5H09_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1255..1986)
                     /gene="mobC"
                     /locus_tag="R5H09_RS29125"
                     /old_locus_tag="R5H09_29125"
     CDS             complement(1255..1986)
                     /gene="mobC"
                     /locus_tag="R5H09_RS29125"
                     /old_locus_tag="R5H09_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1986..3950)
                     /locus_tag="R5H09_RS29130"
                     /old_locus_tag="R5H09_29130"
     CDS             complement(1986..3950)
                     /locus_tag="R5H09_RS29130"
                     /old_locus_tag="R5H09_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5434..5583)
                     /locus_tag="R5H09_RS29135"
                     /old_locus_tag="R5H09_29135"
     CDS             complement(5434..5583)
                     /locus_tag="R5H09_RS29135"
                     /old_locus_tag="R5H09_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5668..5925)
                     /locus_tag="R5H09_RS29140"
                     /old_locus_tag="R5H09_29140"
     CDS             complement(5668..5925)
                     /locus_tag="R5H09_RS29140"
                     /old_locus_tag="R5H09_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5935..7620)
                     /locus_tag="R5H09_RS29145"
                     /old_locus_tag="R5H09_29145"
     CDS             complement(5935..7620)
                     /locus_tag="R5H09_RS29145"
                     /old_locus_tag="R5H09_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7947..8192
                     /locus_tag="R5H09_RS29150"
                     /old_locus_tag="R5H09_29150"
     CDS             7947..8192
                     /locus_tag="R5H09_RS29150"
                     /old_locus_tag="R5H09_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8466..8837
                     /gene="tolA"
                     /locus_tag="R5H09_RS29155"
                     /old_locus_tag="R5H09_29155"
     CDS             8466..8837
                     /gene="tolA"
                     /locus_tag="R5H09_RS29155"
                     /old_locus_tag="R5H09_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8834..9199
                     /locus_tag="R5H09_RS29160"
                     /old_locus_tag="R5H09_29160"
     CDS             8834..9199
                     /locus_tag="R5H09_RS29160"
                     /old_locus_tag="R5H09_29160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9374..9652
                     /locus_tag="R5H09_RS29165"
                     /old_locus_tag="R5H09_29165"
     CDS             9374..9652
                     /locus_tag="R5H09_RS29165"
                     /old_locus_tag="R5H09_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9640..9954
                     /locus_tag="R5H09_RS29170"
                     /old_locus_tag="R5H09_29170"
     CDS             9640..9954
                     /locus_tag="R5H09_RS29170"
                     /old_locus_tag="R5H09_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             935..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4753..5331
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1259..1986)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tacatttacc gtgagaaatt acacaccttt cacacccgat aaaatcaggg gtatacagtg
       61 gcgaaaacat tgctcaacct ttcacaggca gcacaagccg cgggcattac cagaagaacg
      121 ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct
      181 gtcgtggatg tgtcagaact gatccgtgta tacggaaatg tgaatatccc tgagaaacaa
      241 attcccggca tttcacaccg tgaaaataca caaaaaaatt tcccacagga acagcttctg
      301 gcaatgcaaa aggaactggc cgatctccgg caggctgtca ccctgatgct ggaagataaa
      361 acatcccggg aagaagaacg ccgccagcac gatgatgagc gccggaaact ccaggccgaa
      421 gtggacagat tgacaacaga actgacacag aagaaaaaac gcttctggtc cggctggttt
      481 agctgagaac ttaccgaagg gaatggagaa catgacgaag caggaaaaaa ctgccctcaa
      541 tatggctaaa ttcattaagg atcagtcgct gctgttgctg gaaaagctga acgagctgga
      601 catggatacc ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa cactctggca
      661 ggtgatgcgg gataagctcg gggaagaata acagcaccgg ccatgccggt gctggcgtta
      721 ttttttgccg aacggccacc accggcgggc gggtttgtcc tccggcagtt caggcaccgg
      781 cacgttaaaa tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac
      841 cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct gctccggtga
      901 gcacttcccg gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccacctttcg
      961 atagaggtaa tcacacagct tcattcctgt gggtttgtcc cgttcccatt gcggattttc
     1021 accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat
     1081 ggatttcatc atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt
     1141 ctgccaggcg agcaccacca tgtcttccag atgctcgctg gttggcataa tgcagcgctc
     1201 gccgtctttg tcttccacgt cctcaataaa taccatgtac agcgccataa tccctcactc
     1261 cgttgtcata aatttaaagc acgcgaacag cctctcccgg tccagctcca ccgttttgcg
     1321 ggcctcgccg gtctgtggat tcacgatttc atccaccgcc agaatcgccc gctgtacccg
     1381 gacaagcgtc ggccggtcag ggcagatgta ccagatttca tcgatgccgt tcgcccggcg
     1441 gttgaacaga tacccggaca ccacttcctg ataccgcctg cggctcttga cggtgcgctc
     1501 tacctcaaat gccaccgttc tgccgtccag ctccgccagc gcgtccggca ctttcatccc
     1561 tttcagggcc atacggtgca ggtaccgcca gccggtggcc ccccggccct ccatggagag
     1621 gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc ttgacggctg
     1681 aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca ccccgtgcgg
     1741 cgtcagcccc cacacgctga cctgacggcc gtcaaccggg tatttcgccc ggaccagcag
     1801 ccctttcgcc accagactgt ccaggaagcg gtaaaccggc tgtaccgccg caatccccag
     1861 ccgtcgcgca accagctccg ccgtggaaca cagctcttcc cgcagccagc gcagggtcag
     1921 ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg ctgacgttgt actgctccag
     1981 tgtcatcagt aaagccccgc aaaatcatca tccgcttttg gtccgccgcc gtcggccgga
     2041 ggcaggtact ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt cagcgaaatt
     2101 tcccggcgct caacccgcag cgggctgctg aacgccagcc gcgccacgcc cagcccggtc
     2161 atcaccgcga acccgtcagg caggtgttgc agggtgttca cgtcaaacag cggccgcgcc
     2221 tccttctgga tgaagctgtc gccgctgatg tgctccacgt tccccgcctc ccggctggcc
     2281 cggcggcgct ccacgtccac ccggatctcc ccggtctggc ccgccgccca ctgcgcggac
     2341 tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac cgtggttttc
     2401 acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga ctggtgcgcg
     2461 agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac gtacttcgac
     2521 agcagatatt taatctcatc gagcatgatg ctcgcgtgcg gccacctccg gaactcatcc
     2581 cgcgcaatga tgatttgcgc gcagcgggca aacagcatct tctgcacccg gatcaccgcc
     2641 tcatcgtcca tcgagccgat gacgtacagg cagccgccgc cgttgattat ccccgccaca
     2701 tcgataccgc tgtccgtctg caggacgctc aggtcggcca cgttctccag cttggtgatc
     2761 agccccttca cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc cagcacctgc
     2821 tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt ctgctcgctg
     2881 atgcggtagt ggtccgccac gtcgccggtc tcactcaggt taaacccggc aaccagcaga
     2941 ttcttcaggg cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc
     3001 cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg cgcccacgcg
     3061 tcccctttgg ggtcgaagta caccacagcg tccccgaaat cgcgtacgca gcgaatgagc
     3121 gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc cgaggatctg aatattggtc
     3181 ttgcgccagg tatcacgagg gaccagcacc ggctggccct gctcatcctt acccagaaac
     3241 accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc
     3301 ggctccccga aatgcaggtg gcggacgtct gtcatttctc cggtccgctg gctgctgcgc
     3361 cggctctcct gattcagttt cgcgttcacc gcctcgtcag cggccgcaaa atgaccatca
     3421 agcagcagga acgccagcag ccgcgccaga aggacgccgg cgacaagccc tccggcacac
     3481 caggctatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt cgcatagagg
     3541 tccgggcgca ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc
     3601 agaagggcca gaccggcagc gccggtcagc cagtacgcgt agttccagac atgtcgccgt
     3661 gccctgacca tccgcccggg tgaatccagc gaggccagca ccgaaaatgg cacagtccgg
     3721 ttattatcgc gcttatagcg atcggcatac tcccgccgcg ccttatggct cagccccaca
     3781 aaacgcattc ggccggccca gagttcagcc agaccgaaaa acaggccgat aaccagaaaa
     3841 aaaccggcca gactgtaata ctcccggtac agccagctga cccacgggta aatctgccgg
     3901 gccagctccg ggatatgcca ctgccaggca agcgaatccg tattaaacat gcaatctctc
     3961 tccttcagac gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa gccgcggaca
     4021 tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg
     4081 gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac
     4141 gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc
     4201 cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct
     4261 gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg
     4321 ggggccgggt gaatttctgg cgtggccggc gtccagccgg acagagccag aaagagggga
     4381 aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag gtcttttccc ctgaagtcag
     4441 ccacgcccca gcggggatga cttgcatttc tccgcaagga gaaaaaagca acagagatag
     4501 gttaacgtaa aatgttcagc gaaaaaccga catggttcac ctatcctgat aattgatcgt
     4561 cagggaatag aaagacttaa tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt
     4621 cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg
     4681 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
     4741 gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc cataggctcc
     4801 gccctcctgc cagagttcac ataaacagac gctttttcgg tgcatctgtg ggagccgtga
     4861 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
     4921 tttccggtag gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
     4981 ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct catagctcac acactggtat
     5041 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag
     5101 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccggg aaagcacgca
     5161 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     5221 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     5281 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     5341 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     5401 aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc
     5461 agaggaggac gatggtgcca ccgttccgcc ctgaacatcc cggatataat ttgcctgaca
     5521 tgcggccaga agaaagccag caacaatcaa tataaaaaga aaaatagtct ttaccttttt
     5581 catatacttt ccctgtattt aaagactccg gcattcatcc ctgaacaccg gaagtcagta
     5641 tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat caaaagctat
     5701 ataacaatta tccacatcag gttttatttg atttttaaaa aatggctgta gtaaaggaac
     5761 ccattctttc ttcaaatcaa actcaccatt attaatatta tcctttaaag gcattcccaa
     5821 gctttcaatg acagaaccat cgtcaccaaa gtctttagaa tactcttccc ctttaaactc
     5881 ttcagttttc ttatcaaacc atgtcaaacg taatttaagc cccatattta cctctcaaag
     5941 atatttttta atgttccgtt ttggatctgg ccctttaacc tgcttacccg ttttgggatc
     6001 aaatgcgcct aggtgttcgc catcactggc acgataccct tcaagctcac cgtgctggga
     6061 atcccattca taaattttac gttttttatc gccataccat cgggcccgct taccaccacc
     6121 accttgttta ggtgttttag gatcacctcg ctttaactct ccaagtccct ttatgtcttc
     6181 tgttttaggt actggatgat aatcatggcc gtagtcttta gtccccttgc gaggcttgtt
     6241 cttttcctca tttaacttat tttctgcatc cttcgctttt ttctcctttt gtttccggct
     6301 ctccatcgcc ccggtaagtg cagccttatc tctggataag gattcattca ttgcgtttaa
     6361 agatgcctga gccgctttta atttctcatt ctgattctga atatccttat caagatagcg
     6421 aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca caaaatcatc
     6481 tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag cccttgcacc
     6541 ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat ttttatcttc
     6601 ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt ccggatgagc
     6661 ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta cctgtgccgg
     6721 ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt tcgggaaacg
     6781 aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg aatcaccttt
     6841 ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg gtacgctcac
     6901 ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt ttgtcggttt
     6961 cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat gctgccgctc
     7021 atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt ccagtggtaa
     7081 agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa tttttgacat
     7141 cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg caatccccca
     7201 cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg cggccgacaa
     7261 cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca gggcaggcag
     7321 accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc tatgattgcc
     7381 gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc ccggattata
     7441 gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc
     7501 cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat tcccactggc
     7561 ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc caccactcat
     7621 accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatactg tacatataca
     7681 cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag attagttctc
     7741 atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa
     7801 aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac atttcagatg
     7861 gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta aactgttcgg
     7921 gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc gctgaagaaa
     7981 agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa gggaacaaaa
     8041 cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc attcaggacg
     8101 aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct acggaaacca
     8161 ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg tcgcattgcc
     8221 cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac aggcaacaaa
     8281 ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca tgcctatgca
     8341 cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa
     8401 tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga caggtcatca
     8461 acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta ctcctgacgg
     8521 cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag tcatctaaaa
     8581 tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt gaaccagagg
     8641 cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc actctgaaca
     8701 gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca gccgttaccc
     8761 acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa aacgcccctc
     8821 tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt tcctcactcc
     8881 ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc cggtacaggc
     8941 cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg acgatggacg
     9001 agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc tgacggcaat
     9061 gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa attttgaatt
     9121 cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa agctcaatga
     9181 tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc ctggatgatg
     9241 agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc agcaaaagcc
     9301 gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata catatgacta
     9361 caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac ttaaggaccg
     9421 gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc tggaggcaat
     9481 caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg gacaggccgc
     9541 atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg aagcctggat
     9601 aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt aacgatttcc
     9661 gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa aaatcggctg
     9721 gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa aacattgcca
     9781 gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt gatcgggttt
     9841 ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca gattgtgatc
     9901 gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct ctaataaccg
     9961 atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga
    10021 aaaccacatc ctgaacggaa aaacgggtaa aattcagcga
//
