LOCUS       NZ_CP137336             2683 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Proteus mirabilis strain PM16 plasmid pPM16-2, complete sequence.
ACCESSION   NZ_CP137336
VERSION     NZ_CP137336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37919048
            Assembly: GCF_033215415.1
KEYWORDS    RefSeq.
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Li,X.
  TITLE     Proteus mirabilis PM16 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 09:58:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,643
            CDSs (total)                      :: 3,533
            Genes (coding)                    :: 3,482
            CDSs (with protein)               :: 3,482
            Genes (RNA)                       :: 110
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 12 of 51
            Pseudo Genes (incomplete)         :: 42 of 51
            Pseudo Genes (internal stop)      :: 7 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137336.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PM16"
                     /isolation_source="ascites"
                     /host="Homo sapiens"
                     /db_xref="taxon:584"
                     /plasmid="pPM16-2"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2017-10-27"
                     /collected_by="Longjie Zhou"
     gene            138..578
                     /locus_tag="R3372_RS18170"
                     /old_locus_tag="R3372_18170"
     CDS             138..578
                     /locus_tag="R3372_RS18170"
                     /old_locus_tag="R3372_18170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015063446.1"
                     /translation="MSQLFRLASPEDYEYIEELNLWELSFDKRPVQGVRCEDPVIGAEK
                     YNQGREKFKALASRHKKHWKPNGLTFSELIRWAIECGTPEQCQLVLALYHCTNDDDYQA
                     MGIQLSRSIDNANVSPIIFFTGKNQRLLGDISMLKQLEEKVR"
                     /name="hypothetical protein"
     gene            1539..2183
                     /locus_tag="R3372_RS18175"
                     /old_locus_tag="R3372_18175"
     CDS             1539..2183
                     /locus_tag="R3372_RS18175"
                     /old_locus_tag="R3372_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     ARG             1540..2180
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1290..1445
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
ORIGIN
        1 catctggagc ttggtaaacc tcataaattc aattcgcaac gctcgccgca aggcgtgaac
       61 ttgtttcacg tcgaggaagc ggaatttctc gaaagcctta tactgtctcc gttcacacat
      121 gatcggaggg tgtctctatg tctcaattat ttcgtttggc atcgccagaa gattacgaat
      181 acatcgaaga attaaattta tgggaattgt catttgataa gcgaccggtt caaggggttc
      241 gttgtgaaga tccggtgatc ggggcagaaa aatataatca agggcgtgaa aaatttaagg
      301 cgttagcgtc acggcataaa aaacactgga aaccgaacgg attgaccttt tcggagttga
      361 tccgctgggc aatagaatgc ggaactccag aacaatgtca gcttgtttta gcgttatacc
      421 attgcacaaa cgatgatgat tatcaggcga tgggaattca gttaagccga agtattgata
      481 atgcaaatgt cagtccgatc atctttttca caggtaaaaa tcaacggtta ttaggtgata
      541 tttcgatgct aaaacagctc gaagaaaagg tgcgctgacg ttaggtttac ctattatttt
      601 atgtattatc ctatctatta ttctatctct tattttatct atttcgtctg taataggtag
      661 aataatacat agaataagat acagaataat agatagacac aagatgacgt tgtgttattt
      721 cttacccatt aatccacctg ttattctacc tattacttta cccgttattg cacccaattt
      781 aggtataacg ggcagaataa cgggtgaaat aatgggtaga atcatagttt tactaacttg
      841 caccccatga tactttcagt atcaatgcca ctcattttct ggacgaaaat gaatgacata
      901 aggctccgcc ctctggctat tttcttcggc tggacgcctg cgaaaaaaac cattcaccca
      961 agcccgtcaa actcactgcg ttcgtctgac cacccccttt tttcgcccct gtatttggtc
     1021 gctcaaaaat gtggtggtca gacgcttcgc ttgacggggg gctttgcccc caaaacgaaa
     1081 cacgttcact ggggttcact tgccatcgct ggggcgtgtc atttcgtttc ggctccccct
     1141 gtttttccct ttcttcgctg gacgctcatc aggataaaaa caaaaagcct accgccctcg
     1201 ttttactcgg ttggtcaaat cgcttcttgc cacaaaacaa gtttgtgact ttcagcgatt
     1261 tcgcatcgtt ttacttgtca gtttgcacga tgttaaaaac gcactcagaa gcccgcaaaa
     1321 ccgttctaaa ctttcagcgt agatttgggt gttttatagg gtgatcgtgt cttaaaatcg
     1381 tttcgagggg ctttaatggg gatgaacggg aggtttttgc taactaactc gcccgtttaa
     1441 cataaaaggg attgtatgaa tcaattttag ctagagttta aggttgttca aattaatgta
     1501 caatgattgc actgtataaa taaccaggtg tagcatgtat ggaaaagcac tttatcaatg
     1561 aaaagttttc acgagatcaa tttacgggga atagagttaa aaatattgcc ttttcaaatt
     1621 gtgatttttc aggggttgat ttaactgata ctgaatttgt tgattgtagt ttttacgaca
     1681 ggaatagctt ggaagggtgt gattttaata gagccaaact aaaaaacgct agctttaaaa
     1741 gctgcgattt atcaatgagt aattttaaaa acattagcgc cttaggtctt gaaattagtg
     1801 agtgtttagc tcaaggagct gattttcgag gggctaattt tatgaatatg ataactacaa
     1861 ggtcatggtt ttgtagtgct tatataacca agacaaatct tagttacgct aatttttcta
     1921 gagtcatatt agaaaagtgc gaactgtggg aaaatcgctg gaatggcact gtgataactg
     1981 gcgccgtgtt tcgtggctcc gatctttctt gtggggagtt ttcatcgttt gattggtctt
     2041 tggctgattt tactggttgt gatttaacgg gtggggcgct tggcgagctt gatgcaaggc
     2101 gaattaattt agatggagtg aagttggatg gagagcaggc gcttcagctt gttgagagtt
     2161 taggtgttat tgttcaccga taaaatctag gtaaaaaacg cctaatgccc caatgtggta
     2221 ctaatcaaaa cggagcgaag cgacatcttc atcttgtctt tgcttttctc catgatcagt
     2281 tattgggtca tggtctctta gctcgttaat ctggctaagt ttggttaggt tgggcttctt
     2341 tcgacacttc gcaatccaag aaatccctag tccgttgatt ggtgctctta tcaggtacgc
     2401 actgtaatct ttcgataccg cagcgtgatt cggaggtaga gccggaattt cgttttccgg
     2461 tacgttttac taccacaatt tgcctgttta tcctttcgga gaccgggcta agtccgagtc
     2521 aatcaagtgg tgcatttggg ttaatgtcgt tggctggtat ttaatgagaa cgtgacaagc
     2581 gcctctcacg ttgctaagat tggattgcga cgtctaatct gagcaacgaa tgcacaagat
     2641 ttagagtacc agagccctgt gatgctgtca cggggctttg tta
//
