LOCUS       NZ_CP137192             2155 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Citrobacter freundii strain F1917 plasmid pF1917-11, complete
            sequence.
ACCESSION   NZ_CP137192
VERSION     NZ_CP137192.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37933681
            Assembly: GCF_036289145.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Li,X.
  TITLE     Citrobacter freundii F1917 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 02:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,708
            CDSs (total)                      :: 5,588
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 31 of 132
            Pseudo Genes (incomplete)         :: 94 of 132
            Pseudo Genes (internal stop)      :: 25 of 132
            Pseudo Genes (multiple problems)  :: 17 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137192.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="F1917"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pF1917-11"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2017-11-22"
                     /collected_by="Jiayao Yao"
     gene            join(2058..2155,1..103)
                     /locus_tag="R4T16_RS26545"
                     /old_locus_tag="R4T16_26525"
     CDS             join(2058..2155,1..103)
                     /locus_tag="R4T16_RS26545"
                     /old_locus_tag="R4T16_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024148903.1"
                     /translation="MNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRHIVSDTNQAR
                     RSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(151..339)
                     /locus_tag="R4T16_RS26550"
                     /old_locus_tag="R4T16_26530"
     CDS             complement(151..339)
                     /locus_tag="R4T16_RS26550"
                     /old_locus_tag="R4T16_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(594..896)
                     /locus_tag="R4T16_RS26555"
                     /old_locus_tag="R4T16_26535"
     CDS             complement(594..896)
                     /locus_tag="R4T16_RS26555"
                     /old_locus_tag="R4T16_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_023207139.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGVHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(1204..1317)
                     /locus_tag="R4T16_RS26560"
                     /old_locus_tag="R4T16_26540"
     CDS             complement(1204..1317)
                     /locus_tag="R4T16_RS26560"
                     /old_locus_tag="R4T16_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          1680..1852
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="100.0"
                     /evalue="2.04e-62"
     Typing          complement(1209..1755)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.625"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gtatcgccat attgtgtccg ataccaatca agctcgtcgt tcctacatgc tcgacctgct
       61 cgaaagcgtc aggcatgact atgtacacgg tggatataca tagttgccct ggatacgctt
      121 ttcgtctaag tgagcgttct tttcgtctct ttattcgccg tcctgtatac cgaagcgtgc
      181 gctcagcttc tgataaagcg cttctgcctg ttcgtgcatt cgctcacagt cggctgcctc
      241 gtcatcaaga tcgagcacgt cgagtttttc gagcagcagc agcgactgcg cccggataaa
      301 ttttgccatg ttcagtgcgg ctgattcctg ttttgtcata cggctttttc ccggttttat
      361 ggtgatcgca gactgaaatc ttacttcatt cacttgagtg catagccatg caggtacgca
      421 gaaatcatca ggggcgtttt ttctcgatat gggcacagaa aggtgtatgt ttcaggccgg
      481 agtcctcaga aacgcgctga gggcgtctca ggcggtgcgg gcaacggatg ttatggtaaa
      541 tctgcctcac agactggtac aaaaaaagcc ccgcagggct tttttgctat gagttagcca
      601 tattttcttg cgaatttttc atcggaatcg catagataaa taataaactc aataaacgcg
      661 atgatcgcgg gtatgaatgt ccagcaaaaa attagataca ggaatccctg tcctatttta
      721 cctaagtaaa atttatgtac gccaaatcca ccaaggaaga atgctaatag cgcggctgcg
      781 attctacttt tttcacctga acctgaagag gcgtttgtgg ctccgcagtg tgggcatgac
      841 tttgctgttt cgtgtatttc cttgccgcat cctcggcagt aaaccattga actcattttt
      901 tatcctcgag cttattttta gttgggatgc ataatatccg atgttttata acgatatgca
      961 tcctatatgc tcgggtggga tagatttcca ttatggtgaa ggatcttctt gagatccttt
     1021 ctgtagcgtc gtaatctctt gctctgtaaa cgaaaaaacc gcccagcaag gcggtttttc
     1081 gaaggttctc agagcagcaa ctctttgaac cgtggtaact ggcttacgag gagcgcagga
     1141 accaaatctg ttctttcagt ttagccttaa caggcgcata acttcaagac taactcctct
     1201 aaatcagtta ccagtggctg ctgccagtgg cgctttgtcg tgtctttccg ggttggactc
     1261 aagttgatag ttaccggaaa aggcgcagca gtcggactga acggggggtt cgtgcataca
     1321 gtccagcttg gagcgaactg ccttcccgga actgagtgtc aggcgtggaa tgagataaac
     1381 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     1441 gggagctacc agggggaaac gcctgggatc tttatagtcc tgtcgggttt cgccgccact
     1501 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     1561 tcgccgcggc cttatcgttt ccctgttaag tttcttcctg gcatctccca ggagatttcc
     1621 gccccattcg taagcccgca ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaatatc tcctgtctca tatattccgc ttacgctctg ctgctgcatt
     1741 ttcctcctgc cacatgaagc acttcactga ttcccgcacc cgtgcctaca tagtaagcca
     1801 gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaca cccgctaaaa
     1861 gcactgacgc gcctgcggct tgcccaaccc cgcgccgtcc gggaaagaat ttcccgatcg
     1921 acccgacgtt tctgtttacc tgtgatgtta acgtttggat ctttttcccg ggtttaccta
     1981 ttttcttcta atgaagcgtt cacagctgac gttaatagat gggtgctttc atgtttggtt
     2041 tttcaccagg agaacttatg aacctttgcc ccgatgaacg tctgctcttt gtgcggatga
     2101 tttccgctat gcttcgccgt tcgggcggtg atgcgggtgc tgttatgttt gaggc
//
