LOCUS       NZ_CP137187             2182 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Citrobacter freundii strain F1917 plasmid pF1917-10, complete
            sequence.
ACCESSION   NZ_CP137187
VERSION     NZ_CP137187.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37933681
            Assembly: GCF_036289145.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Li,X.
  TITLE     Citrobacter freundii F1917 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 02:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,708
            CDSs (total)                      :: 5,588
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 31 of 132
            Pseudo Genes (incomplete)         :: 94 of 132
            Pseudo Genes (internal stop)      :: 25 of 132
            Pseudo Genes (multiple problems)  :: 17 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="F1917"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pF1917-10"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2017-11-22"
                     /collected_by="Jiayao Yao"
     gene            complement(join(2078..2182,1..132))
                     /locus_tag="R4T16_RS25450"
                     /old_locus_tag="R4T16_25420"
     CDS             complement(join(2078..2182,1..132))
                     /locus_tag="R4T16_RS25450"
                     /old_locus_tag="R4T16_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            820..978
                     /locus_tag="R4T16_RS25455"
                     /old_locus_tag="R4T16_25425"
     CDS             820..978
                     /locus_tag="R4T16_RS25455"
                     /old_locus_tag="R4T16_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(1215..1583)
                     /locus_tag="R4T16_RS25460"
                     /old_locus_tag="R4T16_25430"
     CDS             complement(1215..1583)
                     /locus_tag="R4T16_RS25460"
                     /old_locus_tag="R4T16_25430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048232015.1"
                     /translation="MALLISLVVVAGLYYQFWYKPRRTPILRVWGIAPNGQRFRLLLTE
                     HGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYLEDAHKNELLIELNESDLYPGGAIEFQ
                     QKGGRKINCEILKVRWSR"
                     /name="hypothetical protein"
     gene            complement(1777..1953)
                     /locus_tag="R4T16_RS25465"
                     /old_locus_tag="R4T16_25435"
     CDS             complement(1777..1953)
                     /locus_tag="R4T16_RS25465"
                     /old_locus_tag="R4T16_25435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164714441.1"
                     /translation="MPETALMRQDCAEGACGGAGKGCYGKYSYFVRTKEIGEIHSAAPI
                     YEEILKYEKLDSS"
                     /name="hypothetical protein"
     Typing          complement(298..471)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          395..929
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.182"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 cgcacgcgct ctgttcagga cgatgtgccg gttaggctga tttagctttt tacctgacgc
       61 tctttcagct gcggcaacag catcatcaag aaattttttc tctgcaggcg tcaagtttgt
      121 cagtctggcc ataacgttct ccagtcagaa taaatcagca cagtctgcca gacacagtag
      181 aaacgatgaa gcgccagcga cggtgaaagg cagtcagaaa cggtgaacgg gagcggattt
      241 taatccggtg ccgtgaggcg ttggacaagc cgcaggcgcg tcattagctt ttagcgggtt
      301 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttactatg
      361 ttggcacgga tgcggaaatc agtgaagtgc ttcatgtggc aggagaaaaa aggcggcaac
      421 ggcgcgtcag cagaatatgc gatacaggat atattccgct tcctcgctca ctgactcgct
      481 acgctcggtc gttcggctgc ggcgagcggt accggcttac gggcgtgacg gaaattccct
      541 ggaagatgct tagcagagag gcgataaggc cgcggcgaag ccgtttttcc ataggctccg
      601 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
      661 acttaaagat accaggtgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc
      721 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt gtctcattcc acgcctgaca
      781 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
      841 tccgactgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacacgaa
      901 aaatcgctac tggcagcagc cattagtaac tggaggtata cgagagacaa attgagaggt
      961 cttgaagtat gggcctgaga gaggctacac tgaaagaaca gttttggttt ctgcggtctc
     1021 gtatacccag ttacctggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc
     1081 tccccaggtg gttttttcgt tttcagagca agagattacg actgtaacga caggatcaca
     1141 agaagatcat ttactccgag agagtaacta ccaccatgtt tgcctctcaa cacacttagg
     1201 aaacattact tgagttaacg actccacctt acttttagta tctcgcaatt aatcttccga
     1261 ccgccttttt gttgaaattc tattgcgcca ccagggtata aatcactttc atttaactca
     1321 attaaaagct cgttcttgtg agcatcctct aaataaaggt aataacaccc accataaagg
     1381 ttctgcgtat ctggcacggc aacaaaagat ttctttccga acagtaaaac tgcctctttt
     1441 tctccatgct ctgtcagcaa taatcgaaat ctttgtccat ttggcgcaat cccccacaca
     1501 cgtaagatcg gcgttctacg gggcttatac caaaattgat agtaaagccc tgccactaca
     1561 accagtgaaa ttaataaggc cattttaatc ctcaaaaaat acagtatccc ggaatttttt
     1621 tattcatttg tactgcttgg aatagtgatc caaaagataa cacctgaaac taaaagaatc
     1681 gacccagcta catacgttac ataaacccca acataagaga ccgtcagcca tataagtaag
     1741 tatatagcta gggtaaatcc aaaaataatt ttggccttat gatgaatcca gtttttcata
     1801 ttttaaaatc tcttcataaa ttggcgcagc tgagtggatc tcaccaatct cttttgtacg
     1861 cacaaagtag ctatatttac cataacatcc cttgcccgca cccccgcagg cgccctcagc
     1921 gcaatcctgc cgcatcaggg ccgtttccgg cacctttcct caccagtatc ccggaaaaac
     1981 gcccctgatg atttctgcgc gactgcatgg ctatgcacgc aggtgaatgg caggtgattt
     2041 cgcagcgtca gcaccactaa accgccaaaa gccaaaatca gcggcgcggc gcgcgcggac
     2101 gtgaccaggc aaaatcggac tgctgccgct catcctcacg caatgcacgc tggcggtcgg
     2161 cgtaacgctg caactcaatt tg
//
