LOCUS       NZ_CP137185             5410 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Citrobacter freundii strain F1917 plasmid pF1917-6, complete
            sequence.
ACCESSION   NZ_CP137185
VERSION     NZ_CP137185.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37933681
            Assembly: GCF_036289145.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Li,X.
  TITLE     Citrobacter freundii F1917 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 02:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,708
            CDSs (total)                      :: 5,588
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 31 of 132
            Pseudo Genes (incomplete)         :: 94 of 132
            Pseudo Genes (internal stop)      :: 25 of 132
            Pseudo Genes (multiple problems)  :: 17 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137185.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="F1917"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pF1917-6"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2017-11-22"
                     /collected_by="Jiayao Yao"
     gene            complement(40..363)
                     /locus_tag="R4T16_RS25065"
                     /old_locus_tag="R4T16_25040"
     CDS             complement(40..363)
                     /locus_tag="R4T16_RS25065"
                     /old_locus_tag="R4T16_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_171853543.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMTIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1012..1158
                     /locus_tag="R4T16_RS25070"
     CDS             1012..1158
                     /locus_tag="R4T16_RS25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001717510.1"
                     /translation="MHEPPVQSDRSVLSGNYRLESNPERHNKTPLAAAIGKRVYKRLLM
                     QSS"
                     /name="hypothetical protein"
     gene            complement(1615..1896)
                     /locus_tag="R4T16_RS25075"
                     /old_locus_tag="R4T16_25045"
     CDS             complement(1615..1896)
                     /locus_tag="R4T16_RS25075"
                     /old_locus_tag="R4T16_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000594459.1"
                     /translation="MIKNIKHKGLRSFAENGNTAGIQPHLAKKLRMILTTLSTATCLQD
                     IVDVRALGCHPLKGERKGEFSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2288..2527
                     /locus_tag="R4T16_RS25080"
                     /old_locus_tag="R4T16_25050"
     CDS             2288..2527
                     /locus_tag="R4T16_RS25080"
                     /old_locus_tag="R4T16_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_164525734.1"
                     /translation="MEPFEIPAAELADKLEVSQSALSRLLNGKTDLSYEMALRLSKVIG
                     RSAESWVNMQAQYSLSKSRKTLSETLEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            complement(4059..4274)
                     /locus_tag="R4T16_RS25085"
                     /old_locus_tag="R4T16_25055"
     CDS             complement(4059..4274)
                     /locus_tag="R4T16_RS25085"
                     /old_locus_tag="R4T16_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4281..4772)
                     /locus_tag="R4T16_RS25090"
                     /old_locus_tag="R4T16_25060"
     CDS             complement(4281..4772)
                     /locus_tag="R4T16_RS25090"
                     /old_locus_tag="R4T16_25060"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_284215137.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGIQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     Typing          complement(477..650)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          574..1161
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.54"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(44..363)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.523"
                     /coverage="100.0"
                     /evalue="3.99e-61"
     Typing          complement(3968..5409)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.617"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 accggcccgg cgccaccgcc gcggccgcgc ggatgaaatt taacgatcat cgtccgttcc
       61 tttctcaaga acggcgtgcc gcagccgctc aaggccggca tcgatggtca tcagcgccgc
      121 cacaatctgt accctgtcat ggccggtgcc gccgccggcg ttaacacggc gggctatctg
      181 gttcaggtta ttgcccatgc cggcaagctg gcgcagcagc gccggcgaga gtgaggggag
      241 ctttccggaa cgtgccggct tttcgtccag gcagatctgc cgcatccacg ccgccagctg
      301 cctgccgtca cagcgctcga gcagccgccg gtgctcgtcc tccgtcaccc acatggtgag
      361 catcttgctc cgtttctctg acaaaccggc ctcctgataa cgccgggacg ggcgggaaag
      421 cctttcccgg tcggcgcggg gttggacaag ccgcaggcgc gtcaggcttt tggcgggttt
      481 cggggcgcag ccctgaacca gtcacgtagc gctagcggag tgtatactgg cttactatgt
      541 tggcacggat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggcggcaccg
      601 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgctg
      661 cgctcggtcg ttcggctgcg gcgagcggaa atggcttacg aacggggcgg aaatttcctg
      721 gaggatgcca ggaagaagct taacagggaa gcgagaaggc cacgacaaag ccgtttttcc
      781 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      841 acccgacagg actataaaga taccaggcgt ttccccctgg tggctccctc gtgcgctctc
      901 ctgttcctgc ctttcggttt accggtgtca ctccgctgtt atggccgcgt ttatctcatt
      961 ccacgcctga cactcagttc cgggaaggca gttcgctcca agctggactg tatgcacgaa
     1021 ccccccgttc agtccgaccg cagcgtctta tccggtaact atcgacttga gtccaacccg
     1081 gaaagacaca acaaaacgcc actggctgcg gccattggta agagggtgta caagagatta
     1141 ctgatgcaga gttcttgaag tcagagccat actgaggcta aactggaagg acagaatttg
     1201 gtttcagcgg tcttgtacac cctgttacca cggttcagaa gttccccaac ttactgaacc
     1261 ctcgaaaaaa ccgcctcccc aggcggtttt ttcgtttcga acataggata cgctttgtcg
     1321 atttaatcag tcaaggaaga accctgaaag cctcaatcgc ttacagaatt attcttgtta
     1381 gagacatgtt tctgcaccgt atcaacacca gtacacaaaa cagctgcaaa caggaaccat
     1441 ccccacccat ctaggccacg aacagcaaga accccagcaa tgactacaca aagcaaaggg
     1501 aacaacaaaa tcataaaggc tctcataaat cctccagagg tacttatttt tgaataaagt
     1561 ccctaatcca tccatattgc cactaggatg gattagtaac ttacttattg attactaatg
     1621 gtaatcttca agatcaacgt ctaagacgtt tggttcttcc accttgaaaa ccaccctcca
     1681 gtttccgtta acctctacgg aaaactcacc tttacgttca ccttttaggg ggtgacaccc
     1741 cagcgccctt acatccacaa tatcttgaag acatgttgcg gtgcttagcg tagtcaatat
     1801 catgcgaagt tttttcgcta agtgaggctg aattccggcg gtgttaccgt tttcagcaaa
     1861 actccgaagc cctttatgct ttatgttttt tatcatcaat catctccata aaattttcgt
     1921 cgatatctat ctctataaac agaaaacaat ttgacagcgt ccatttgaac cccttgcatt
     1981 attaaaataa ttgacgtaac tttgtctctg aggtcgatta cctcgtttga cttccaagag
     2041 cgctaaccgt acaatatggc gccttgaaaa agtcggtaaa gacaccatat cagcggtagc
     2101 tttctgctcc accgagcatc ctaaagctta ctgctttttt tgtcaataac agacacttgc
     2161 gtattgtgca atgcgcaacg cgaaagaaac aaaatgtttt gtgtaatcaa atacaaaacc
     2221 aatagaggaa ggcatgataa atctttacga tcctgtacac cccggtgaat tcatcaggga
     2281 aatgttcatg gaaccgttcg aaatacctgc cgcagagtta gctgataagc ttgaagtatc
     2341 acaatctgca ttaagtagat tgctcaatgg aaaaactgat cttagttatg agatggctct
     2401 tcgactatct aaagttatag gccgctctgc agagtcgtgg gttaatatgc aggctcagta
     2461 cagcctctcg aaaagtcgta aaactttatc cgagacgcta gaacaccttc atccgatgtt
     2521 tccataagta aaagcccgct cgtgcgggct ttttgccgta aaacccccgc atacctacct
     2581 ttagtttgat ttttagttca gttcttagat cttgaaatga ttgttgtttg aacaccaata
     2641 agatgcccat acaaggctct aaaaaggaga acaaaatggc aatttatgta ggaccacatg
     2701 gcaaaatagg tcgtgatggt ggctcattcc aagaatgtga catcaacaca aacactcata
     2761 ctgttacccg caaatatgga cgatactgca ccattcgcga taatgagttc gcaccaccaa
     2821 cgagcgcacc tggcaggtac tggctacaga tctaccgaac cccagacaac actgagtaac
     2881 agaagcgcca gtaacgacaa agcccgcata agcgggcttt gtctcttaca aaattcctcg
     2941 tcgtcgcaat tcagcttcta tcgcctcaca tgcggcatcc acaatctccg tgttgtagta
     3001 cctggacatg ccactgctaa tgatatcgtc cattagggca cggttatgag ctaaggtacg
     3061 tcgtaagtat tcaggggtat gttgctccat cggattctcc ttttcatgac tgagcattat
     3121 tgcttacctg tatttatccc cgtcagtgct ggtcacgttg aaaagcccta aaaaaccact
     3181 tttttagctg ccttaatcat tgaaaaatgg cacttaaatc cacatctatc gcgctgggga
     3241 aatcgcccgc acacagccac tgataaaata aaagtgagat aaaatggatc caatggataa
     3301 gatttgcgat gtttgtgccc tccctgccag cgcctgtacc tgtggcttct gcgatgactg
     3361 caacgagaag ctggcagact gcgtctgtga gcgctgcagc gattgtggaa atttagatga
     3421 gcactgtagc tgctcacgtt gcgacgagtg cggtgaaaaa ctggatgagt gcatatgtga
     3481 ggagtgcgaa gaatgtctcc gcagtccaca tgattgtatc tgcgaaaact gacctaaggc
     3541 cgaattctct ttcaagagcg accgggacgg tcgctcttat ttaccataac atccgttgcc
     3601 cgcaccgctg agacggggtt ggctgcgttt ccggtgccag actcaggaaa gtggcgacag
     3661 acatgacgaa aaaacaccag aaacgcagcc gctggatatt acactagtgc atagtcatgc
     3721 agccagatgc atggatgtta aagtcacact aaaatccgcg ccaatgcctt aaaaaccgac
     3781 aaatgcttct ccagccagtc ccacagctcc gccgccagat acaggcagtt aagcaatgat
     3841 tcaataagat ttaacagctt catttcccgc tcctgaataa gtcgtgacct ttttgatcac
     3901 gaacctattt agcgggagaa ataagagccc ctcttcgtgc gggttgtcag ctgcctggat
     3961 ggtttaaagt gtcggcccgc ggtaggtttt ctgcggctgg cgctccagct ccagtgtttt
     4021 ctgatactca ttctccctct gccggagctc gatggcattc agttcccgga caagcggcgc
     4081 agctgctcgc ttaaactcag cacctgcgtg ctcaagtccg acacgcgctc gttcagcacc
     4141 gcgttctccc gttgcataag cccacacatg ctccgccatt ccgcgaaggc gctctcccat
     4201 tcgtccagcc tttgcgagta gtcctgctgt agctgctcta atgcgctcag caactgcttt
     4261 tccagctctg tcattttcag ctactcctgt ttcaggcgga tccatgtcgt cccgtcgtaa
     4321 gggatggctt cggtttgtat ccccggcggc aggataagaa acctccggcc gtcgctgctc
     4381 tcctgatagc gcactcccca cgtccgggcg gtcattttcg ccagggtatc ttcctgctga
     4441 cgaagatgcg tgtaattgtc ggttatctgc tgcccctgcc accacagaat gctcccgctc
     4501 gtggcgatca gcagcgctga caccatcagg atggtgagcc atgtcctccc ggccgtcagc
     4561 aacacgctcc gccggttttt ctccatcgcc tcgctcatac tctgctcgtg ggcgctgatg
     4621 gcgtcgctga ttctcctcgc gcttgcgttc agttccgctc tgacagactg ctcgtgctcg
     4681 ctgaatgcgg ctttcagcat ctcgccggta ctcttctgct gcgctttcga ttgctgctct
     4741 aagtccttcg ccagcgttaa aagactgctc atagatggct cccttcagtc ggatgtttcg
     4801 ccccccgtca gggtcggcaa tgctgatact gcttttcgtt gtacgcacca cctcaaaacc
     4861 tgcaccttca agagcctcag tgacgtcctg acggcttttc agctccccgg acgaggctaa
     4921 gctcagcagt ccacgggtga tggcttctgc ggcttcctgc ttcgttttcg gcagcccgga
     4981 cggcgtgacc agcgcccggc ggttttccgg cgcgttcgga tcgtgcagcc ccagtctgcc
     5041 gttcacgatg gtctgccagg catcgatacg cggccggtcg gcgcggtcgt agtacggctg
     5101 gagccgcctg ccggtcagca gctcggtatt ggggatcaga aagttcagct ccagacgccc
     5161 cttgtccgcg tgctccaccc acagaatgct gtactgatct ttatcgagtc cgggcatcag
     5221 aacccgctcg aagctggcca tcagtttttc ccgctggccg ggaggcagat cggcctccgc
     5281 aaacgacaga acgccggagg tgtatttctt aacgtaaggc gacgcgtcaa tcagctcccg
     5341 gacttcttcc ggcttcccct gcaggacggt ggcgccttca cgctgccggt ctttacccag
     5401 cagatagtcc
//
