LOCUS       NZ_CP137182             8828 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Citrobacter freundii strain F1917 plasmid pF1917-5, complete
            sequence.
ACCESSION   NZ_CP137182
VERSION     NZ_CP137182.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37933681
            Assembly: GCF_036289145.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Li,X.
  TITLE     Citrobacter freundii F1917 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 02:12:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,708
            CDSs (total)                      :: 5,588
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 31 of 132
            Pseudo Genes (incomplete)         :: 94 of 132
            Pseudo Genes (internal stop)      :: 25 of 132
            Pseudo Genes (multiple problems)  :: 17 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137182.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="F1917"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pF1917-5"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2017-11-22"
                     /collected_by="Jiayao Yao"
     gene            join(8387..8828,1..53)
                     /locus_tag="R4T16_RS00005"
                     /old_locus_tag="R4T16_00005"
     CDS             join(8387..8828,1..53)
                     /locus_tag="R4T16_RS00005"
                     /old_locus_tag="R4T16_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182945396.1"
                     /translation="MAGTVWNDVNTGREVFSMTHLHPQLHKVEVSGVQAEIEISFGFHV
                     FTDQKQVGKVMSFRGETRHFCQERYEGSKTIVQRILSAIENGEYITAFISKGQGQRYYH
                     LNHHDDFILMEIRKPEGKDNFLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     I"
                     /name="hypothetical protein"
     gene            complement(441..581)
                     /locus_tag="R4T16_RS00010"
                     /old_locus_tag="R4T16_00010"
     CDS             complement(441..581)
                     /locus_tag="R4T16_RS00010"
                     /old_locus_tag="R4T16_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062939423.1"
                     /translation="MQEPPVQPDCCALPGNYRLESNPGRHDKAPLAAAIGNWEFAENLF
                     S"
                     /name="hypothetical protein"
     gene            941..1303
                     /locus_tag="R4T16_RS00015"
                     /old_locus_tag="R4T16_00015"
     CDS             941..1303
                     /locus_tag="R4T16_RS00015"
                     /old_locus_tag="R4T16_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889489.1"
                     /translation="MAWHSVSQAQNMTGKSRRTLYRDMASGRLSWRTNTSGHRELETSE
                     LIRVYGEIKNDGTAERHTVSQPDVTVSGSDIRDELRALRQEVADLKSMLLRLEHKQEEQ
                     PETAAPWWRFWQKKST"
                     /name="hypothetical protein"
     gene            complement(1556..4039)
                     /locus_tag="R4T16_RS00020"
                     /old_locus_tag="R4T16_00020"
     CDS             complement(1556..4039)
                     /locus_tag="R4T16_RS00020"
                     /old_locus_tag="R4T16_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934115.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_220384729.1"
                     /translation="MKKNILAGIFFVAMLLPVWHAAAETFTRCHPNETVWAMIQDEQLK
                     NIARLQETKRCQAVNDTYDQWRQNFQAMGDAENAIRSARDHQNWNAVKSTFDNIAPQIN
                     SLHALAEKNSSLLGAGNILALFGGDLNYLYQNAGLVAPASADGLTDNVSQMLTEPDKQV
                     KAAGIAQQLVYQTRVRAQSFIQGVAQNAGNEAQKEYRAQSDKIVEDKAHNFKGDSVEGY
                     FGGFLARISQVYAPVLLFLIIVGIIGALTARRVRLDAFTASVKAVMAAAVPAVALALML
                     IFMPFLPSWVLYAAALAVTFAIYSRSKKILGGLASRCGRFPALASGLRWMGGFVSGLDA
                     DRKQETGAASVNPASHHTHGSSRWGTAAEMRENNHLLPTEKQAGFTLARELSPPPGVDG
                     KFCFTGHVVTVAPTGSGKGIGAVIPNLLTYQGSALVFDPKGENAEVTGRRRREMGQSVH
                     VIDPFGITSGIKESVNILDRLDVTSPDCVAESAVLAESLVVSEKGGDGNHFDESARTLL
                     QGLMLHVSGLPDESRRNLAELRRLITSDEENLLALLADMAASEDVAFGIPARAANTLMG
                     MADRERGSVLSTARKNTAFLDDPRIAAALSHSDFNLSAIKAEPMTVYLVLPANRIAANT
                     RFVRLFIGSVISAVTESKAKPAHNVAFILDEFNVLGYMKSIEDAVSLLRGYGLSFWVFL
                     QDLSQLKSAYPKWQTFLANSAKTFYGTDDYDTAKYVSDTLGKSTVEFETQNSGRNSGGG
                     VSGGGGSMNRGRSSGSSQQFAGRELLTPDEVMRLGPTRPIVILKGEYPYQLTRINYLTD
                     PEYTGMADPNPYYSA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(4087..4389)
                     /locus_tag="R4T16_RS00025"
                     /old_locus_tag="R4T16_00025"
     CDS             complement(4087..4389)
                     /locus_tag="R4T16_RS00025"
                     /old_locus_tag="R4T16_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889494.1"
                     /translation="MAKSRAEKLEELRQKEAQIKAQIQQLKQREQTEERKKDTRRKILI
                     GGAVLAKIKRGEWNYQQLVSLLDNELKNDRDRALFDEMGLKAVDARKESFNPGNK"
                     /name="hypothetical protein"
     gene            4549..6372
                     /gene="mobQ"
                     /locus_tag="R4T16_RS00030"
                     /old_locus_tag="R4T16_00030"
     CDS             4549..6372
                     /gene="mobQ"
                     /locus_tag="R4T16_RS00030"
                     /old_locus_tag="R4T16_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_072052381.1"
                     /translation="MAIFHLSVKTISRSAGRSATAAIAYRTGSRIVDERTGIIHDYTRK
                     SGVEHSEIFLPQNSPDWASNRETLWNAVEQKENRRNSTVAREFELAFPAELDGKQRLEL
                     LRDFCSSLVERHGMAVDAAIHAPGKEGDQRNHHAHVLCSTRRLSEEGFKDKTRELDDFK
                     SGEVNHWRSAWSEMTNRHLEKAGRSERIDHRSLEAQRESAISQGDSAKAAKLDRVPTIH
                     IGYAAAQMEKRGLETERGNEYRQIILRNAEIQPLSRAIQLIDNWRATHDNNSRAGHGSI
                     APVPERDKGRDKRGVRTAQSYTGRVGAVPPPSARGRLRNLSELDMVRFGERSEMLLPGN
                     VSRDVEHQRTDRNQPLRRENTVTSKSKRPEVIRLEWQSLRRDKLTFVQDKAGRIAARGA
                     AHVERQRQKISSHERQRPEQPSGLIGMFKRSAYEQAASTWQTIKNGLDRRLVQLKNRLG
                     FVRDYMRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMAAYRTRVQSR
                     KQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETLSEETKELESGE
                     KEKLSKRDQVLEKFKAKVERDKQNERGGRGR"
                     /name="mobQ"
     gene            6515..6934
                     /locus_tag="R4T16_RS00035"
                     /old_locus_tag="R4T16_00035"
     CDS             6515..6934
                     /locus_tag="R4T16_RS00035"
                     /old_locus_tag="R4T16_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063058.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_182945395.1"
                     /translation="MSEEQRRRGFMKAEASLRLEGIDPSGSPRYESVKARIISGEITYD
                     QGRAEILAFHTGKRAEPTTAGEMLAEEFLKPLNMSQGELAQATGIPEHHIDDIISNRRR
                     LSAEESYKLAEQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(7095..7448)
                     /locus_tag="R4T16_RS00040"
                     /old_locus_tag="R4T16_00040"
     CDS             complement(7095..7448)
                     /locus_tag="R4T16_RS00040"
                     /old_locus_tag="R4T16_00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227664254.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGRRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            7865..8383
                     /locus_tag="R4T16_RS00045"
                     /old_locus_tag="R4T16_00045"
     CDS             7865..8383
                     /locus_tag="R4T16_RS00045"
                     /old_locus_tag="R4T16_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889486.1"
                     /translation="MSVRAQRAGKRNQSERRIELINTLWEGEEIETWDRNDRPRNDGFI
                     TVPRYLPLLGVLMDELSKGSPLSSTYLALWFRVSDEGLIEIRDKTVLALESGFASGRGV
                     TTWTGRMRKLKELGFISCREGSSGEFHNVLIVHPLVAVKKLLDEGKITKGKTYNTFAER
                     VIEVKSSWE"
                     /name="hypothetical protein"
     Typing          complement(473..840)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
ORIGIN
        1 tgaggttcag gtatgttttg gagcagaggt tacaggggaa gaaaatcata taaaaaacaa
       61 aaagcgccca aggcgccttt gttttactgc aaaaatttcg gtctggcctt tcgttatccc
      121 gctcactggc taggagggtt gcccccggca tcagcacccc tctttcaagg ttccggtttg
      181 tgctgacagg tatattgtcc acttaccaat gccacttgtc aacttcacgg gtttgtcatg
      241 atcttcttga gatcctttgt ttatcgtcgt agtctcttgt ccagtaaacg aaaaaaccac
      301 ccggcaaggt ggtttttcga aggttcagta aaccggcaag tttctgaacc gtggtaactg
      361 gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccgtact ttggcgcaca
      421 cttcaagagc aaccgcgtgt ttagctaaac aaattctctg cgaactccca gttaccaatg
      481 gctgctgcca gtggcgcttt gtcgtgtctt cccgggttgg actcaagacg atagttaccg
      541 ggcaaggcgc agcagtcggg ctgaacgggg ggttcttgca tacagcccag cttggagcga
      601 acgacctaca tcgagctgag atacctacag tgtgagctat gagaaagcgc cacatgtccc
      661 gtaagggaaa aaggcggtca ggtatccggt aaagcgacac ggtcggaaca ggagagcgca
      721 agagggagcg attcaccgga aacggtgatg atctttatag tcctgtcagg tttcgccgcc
      781 tctggcttga gcgtctgttt ttgtgatgct cgccaggggg gcggagccta tggaaaaacg
      841 ggtctggcgt ttttctcttt gctttcttac cgcgtgcctg gatgccgtta gcctgctaga
      901 gtgcagcccg tagagtgtgt catttaatca aggtggtgtt atggcatggc acagtgtgtc
      961 acaagcacaa aacatgacgg ggaaaagtcg caggaccctt tatcgtgata tggcgtctgg
     1021 ccgcttgtca tggcgaacta atactagcgg ccatcgcgaa cttgagacct cagaactgat
     1081 ccgtgtttat ggcgagatta aaaacgatgg cacagctgaa cggcacaccg tgtcacagcc
     1141 tgatgtcaca gtatccggta gtgacatcag ggatgaattg cgtgccttac ggcaggaagt
     1201 tgcagacctt aaatctatgc ttttgaggct ggaacataaa caggaagagc agcctgaaac
     1261 tgcggctccg tggtggcgtt tctggcagaa aaaatctact taaccctttt ctctttttcg
     1321 cggctaaagt tgattacgtt atccggtagt gtttcaggct tggtggtctt ttgtgcctcg
     1381 attggcatag cactgtgtct tctgttttgc gtcaccagag cccagataaa ggcacagaca
     1441 aagcggaaag agaagtatat cggtatgcct atcacaaagg cgacggcggt taaggccggg
     1501 agcaagaccc ggccaaacat cagagctaac agaacgccga caacgattcc aaatatcatg
     1561 cgctgtagta ggggttaggg tctgccatac ccgtgtattc tgggtctgtc agatagttaa
     1621 tacgtgtgag ctgataaggg tattcgcctt tcagaatcac aattggtcgg gttggtccaa
     1681 gcctcattac ttcgtcaggt gtcagtaatt ccctcccagc aaactgctga cttgagccag
     1741 atgaacgacc acgattcatt gagccgccac ccccggaaac tccgccgcca gagttgcggc
     1801 ctgaattctg ggtttcaaat tcaaccgtcg atttacccaa ggtgtcgctg acgtattttg
     1861 cagtgtcata gtcgtctgtg ccgtagaacg tcttcgctga gttggccaga aacgtctgcc
     1921 atttagggta ggcacttttc agctgtgaca gatcctgcag gaagacccag aaagacagcc
     1981 cgtagccacg taacaggctt acggcatcct cgattgattt catatagccc agcacgttga
     2041 actcatcgag gatgaatgcg acattatgag ctggtttcgc cttcgattct gttacagcgg
     2101 agatcacaga accgataaac agccttacaa atcgggtatt tgctgcaatg cgattagctg
     2161 gtagcacaag ataaactgtc atcggttcag ctttgatggc tgacaggttg aagtctgagt
     2221 gtgacagcgc agctgctata cgcgggtcgt ccagaaatgc tgtgttttta cgggcggtag
     2281 acaatactga gccacgttcc cggtctgcca tccccatgag cgtgttggcc gctcgggcgg
     2341 ggatgccgaa agcaacgtct tcacttgcgg ccatatcggc cagcagggct aacaggtttt
     2401 cttcatcact ggtgatcagt cgacgtagtt ccgcgagatt acgtcgggac tcatccggta
     2461 atccggaaac atgcagcatc aggccctgta acagggttcg ggctgattca tcaaaatgat
     2521 taccgtcacc gcctttctct gataccacaa ggctttcggc cagaactgca gattcagcga
     2581 cgcaatccgg gctggtcaca tcgagacgat ccagaatgtt cactgattct tttatgccgc
     2641 tggttatacc gaatgggtct attacatgga cgctctgacc catttcacga cggcgacgac
     2701 ctgtcacctc ggcgttttcc ccttttggat caaagacaag tgctgaaccc tgataagtca
     2761 gaagattggg aattactgcg ccaatccctt ttccgctgcc cgtaggggca acagtcacaa
     2821 cgtgcccggt gaaacagaat tttccatcca ctccgggcgg tggggacagt tctcgtgcca
     2881 gggtgaatcc ggcttgcttt tccgttggta acaggtggtt attttcccgc atttctgctg
     2941 cggttcccca gcgtgatgag ccgtgagtgt gatgtgatgc cgggttgacg gatgccgcgc
     3001 cggtttcctg ctttctgtca gcatcgaggc cagacacaaa accgcccatc cagcgaagcc
     3061 cggaggccag tgcggggaaa cgcccacagc gagaggccag ccctccaaga atttttttgc
     3121 tgcgggaata aatggcgaat gttacggcca gcgctgccgc gtacaatacc catgatggta
     3181 ggaaaggcat gaatatcagc atcagcgcca gtgccactgc gggtaccgct gcggccatga
     3241 ctgctttcac tgatgccgtg aaagcatcca gccttacccg ccgtgcggta agtgcaccaa
     3301 taataccaac gatgataagg aacagcagaa ctggagcata tacctggctt atccgggcaa
     3361 ggaagccgcc gaaatatcct tccacgctgt cacctttgaa attatgggct ttatcttcga
     3421 caatcttatc tgattgcgca cgatactctt tctgggcttc gttaccggcg ttctgtgcca
     3481 cgccttggat aaagctctgt gcccgaaccc gagtctgata aaccagctgt tgcgcgatac
     3541 ctgcagcttt gacctgttta tccggttcag tcagcatttg actgacgtta tcagtcagtc
     3601 cgtcagcgct ggccggagca acgagccctg cattctggta caggtaattc agatcaccgc
     3661 cgaaaagcgc caggatgtta cccgcgccca gtaacgatga atttttttct gccagtgcat
     3721 gcaggctatt aatctgggga gcaatgttat cgaacgtgga ttttacggcg ttccagttct
     3781 gatgatcgcg ggcgctgcgg atggcgtttt ctgcatctcc catcgcctgg aaattttggc
     3841 gccactggtc ataagtgtca ttaacggcct gacaccgctt tgtttcctgt agtctggcga
     3901 tgttttttag ctgctcatct tggatcattg cccagactgt ctcatttgga tggcagcggg
     3961 tgaatgtttc ggccgctgcg tgccatactg gcaatagcat tgcgacgaag aaaatcccgg
     4021 ctagtatatt ttttttcacg ctaaatctcc tgatctgatt tatcatccgg taataaataa
     4081 ttggcgtcac ttattaccgg ggttaaaaga ttcttttctg gcgtcaactg cctttaaacc
     4141 catttcatca aacagtgccc ggtctctgtc atttttcagc tcattgtcca gaagactgac
     4201 caactgctga taattccatt ccccacgctt tatttttgct agtacggctc caccgattag
     4261 gatttttcgg cgggtatcct tcttgcgttc ttctgtctgc tctctttgct ttaattgctg
     4321 gatttgcgcc tttatctgag cttccttctg acgcaactct tccaattttt ccgctcttga
     4381 ttttgccatt agtcacctcg ttgattcaaa tttgaacgat aacccctgta ctgatatatt
     4441 tcaacccgaa gcgaaagcaa agggcgcact tatgcaaact cttcgagttt gcgtgcggca
     4501 gggttaaaac cctgcaccca aggtcaacgg aaaaacagga atacgaaatt ggcaattttt
     4561 catcttagcg ttaagacaat cagccgtagt gctggccgtt cggccactgc ggcaattgct
     4621 tatcgtacag gttcaagaat tgttgatgaa cggactggga ttatccacga ctacacccgt
     4681 aaatcaggcg tcgagcattc agagattttt ctccctcaaa actcaccaga ttgggcgagt
     4741 aatcgggaga ctctatggaa tgcagttgaa cagaaagaaa accgtcgtaa ctcaaccgtt
     4801 gccagagaat ttgaactcgc atttcctgca gagcttgacg gaaaacagcg tctcgagctg
     4861 cttcgtgatt tttgttcttc gctggttgag cgtcacggaa tggcagtgga tgccgctatc
     4921 catgctccgg gtaaagaggg agaccaacgt aatcatcatg ctcacgtttt atgcagcacc
     4981 cgccgactgt cggaggaagg tttcaaggat aagacccgtg aacttgatga ttttaagagc
     5041 ggcgaagtca accactggcg ttctgcatgg tcagaaatga cgaaccgtca tctggaaaag
     5101 gctggccgtt cagagcgtat tgaccatcgt tcacttgagg cgcaacggga atcagctatt
     5161 tctcaggggg attcagccaa ggcagcaaag ctggatcggg ttccgactat tcatatcggt
     5221 tacgcagctg cgcagatgga aaagcgcggc ttagagaccg aacgaggcaa tgaatatcgc
     5281 cagattatat tgcgtaatgc tgaaatccag ccgctttcac gggcaattca gctgattgat
     5341 aactggaggg caacccatga caacaacagc agagcaggac atggaagcat tgctccagtc
     5401 cctgaacgag acaaagggcg agataagcga ggagtccgca ccgctcagtc ctacactggc
     5461 cgcgttggag ccgtcccgcc gcccagcgca aggggtcgcc tgcgcaacct gtccgaactc
     5521 gatatggttc gcttcggaga acgaagtgag atgttattgc cgggcaatgt atctcgtgac
     5581 gtggagcacc agagaaccga cagaaatcag cctctgcgac gggaaaacac tgtaaccagc
     5641 aagtcaaaac ggccggaagt tatccggctt gaatggcaga gtttacgccg ggataagctg
     5701 actttcgttc aggataaggc cggacggatt gccgcccgtg gggcggcaca tgttgaacgc
     5761 cagagacaaa aaatatcctc acatgagcga caaagaccag aacagcccag cggcctgata
     5821 ggaatgttta aacgcagcgc ctatgaacag gccgccagta cctggcagac catcaaaaat
     5881 ggcctcgatc gtcgtctggt gcagctgaaa aatcgacttg gctttgtcag agactacatg
     5941 cgtaagtctg gcgcttatga aatgtctacg ccgggagaac ggctggccga ccagctggcc
     6001 gcgaaagaaa atccgatctt ggctgggcag ataaaatcgc tcaatgctca gaagaacgcg
     6061 gaagatatgg ctgcataccg tacccgcgta cagtcccgca aacaggagaa agctgatatg
     6121 aagctggaaa ctatcgaaga actcaaacaa cgtggtgatg cccggagaga taaagccgac
     6181 aaagccgaac gtgcagagct ggaaaaagcg tctcatcagg cttcactgca gaatgaaacg
     6241 ctcagcgaag agacaaaaga actggagtcc ggggagaaag agaaactcag caagcgtgat
     6301 caggttctgg agaagtttaa ggcaaaagtc gagagggata agcagaatga acgtggtggc
     6361 cgtgggcgtt agcggtatgc aacacagcgc caaccatgct gcatacctga cccggccaga
     6421 ctgatcaggc gtcttttcgg gctgaatgca ttgtactggt taacattatt tcatgacata
     6481 tgaggtacgc ctgatattga catatgaggt agatatgagt gaagaacagc gccggcgtgg
     6541 cttcatgaag gctgaggcaa gcctcagact ggaaggtata gatcccagtg gaagcccccg
     6601 ctacgagtcg gttaaagcga gaattatttc gggtgagata acctatgatc agggacgagc
     6661 tgaaattctg gctttccata ccgggaagcg tgcggagccg acaactgcgg gggaaatgct
     6721 cgcagaggaa tttttaaaac ctctgaacat gtctcagggt gaactggcgc aggctacagg
     6781 aattcctgag catcatatcg atgatattat cagtaaccgc cgtcgcctca gtgctgagga
     6841 gtcttacaag ctggccgaac agttccagac cgataaagat ttctggctca acctgcaggc
     6901 tttgcatgac gcatggctga cgcaccagaa atagcagcca ggggcgcgat tagatatata
     6961 atgttccagt ctaatgtagg atgttccatg caggtcagag tattaaaaat aaagccccgt
     7021 ccttaccagg cggggcttta ttttttcctg cccctcaagt gtcataatgg ttctgaaatc
     7081 cggacatcca aaaatcatgt attagcccca acgagtttct ccataccagt catgaagctc
     7141 accagttccc tgcggccatc cggatattct ttgataacgc agttttctgg tgtcgtctca
     7201 tcggcgtaat agacaggaac gccgctgaga agagcttcgc gggcggcttc gccgcagtca
     7261 tgttccagta gtgatagatt aatggtcgcc atgatgactt ctccttcggt aatactccaa
     7321 atggatggtt ttgacccttt gaccttcttt tcttggctaa attgattgcc attcatttgt
     7381 gtgcatatca attcatacca acaaaaactc accagtgcga ttttacggcc ttttaattgg
     7441 gtaatcatgg tcttttgagg cttagggcat aaaaacgcgc tgagggcctc tgaggcggtt
     7501 ctgctaacgg atgttatggt aaatacgagt gaccggcatg gtgactctta atttattttt
     7561 cgatttctcc acccctcctt gccccctaca accccatgga gcacaaggga gcaccggaag
     7621 cacatggagc acggtgagca catggagcac cgcaattaaa taaaaactct ttttaaatca
     7681 tgttaatgcg aattcttttt gtaatatata ttacttataa ctgccgttta tgtaataaat
     7741 attgcatttt ttatggttaa aaaaaataaa aactggaagt aattgtccga gggaatttat
     7801 aaaagttatt gatttataag gattttttgg taaattggct ctcatgcatt aacaggaggc
     7861 gagtatgtca gtcagggcac aaagagcagg gaaacgtaac cagtctgaaa gaagaatcga
     7921 actgataaat acattatggg aaggggagga aattgaaaca tgggatcgta atgaccgacc
     7981 tcgaaacgat ggcttcataa cagtacctcg ttatcttcca ttactaggtg ttcttatgga
     8041 tgagttatca aagggaagcc cactttcaag tacctaccta gcgttgtggt tcagggtttc
     8101 agacgaaggc ctgattgaga taagggataa gactgtgctg gctttagaat caggctttgc
     8161 cagtggaagg ggggtaacaa catggacggg gagaatgcgg aaattaaaag agctgggatt
     8221 catatcatgc cgggaaggta gctccggtga gttccacaac gtgttgattg ttcatccgct
     8281 ggttgcggta aagaaactac tggatgaagg gaaaataacg aaagggaaaa cctataacac
     8341 ttttgctgaa cgggtgatcg aggtgaagtc ctcatgggag taagaaatgg caggtacggt
     8401 atggaatgat gttaatacag ggcgtgaagt tttctcgatg acacacctgc accctcagct
     8461 gcataaggtt gaggtttctg gggtgcaggc tgagattgag attagcttcg ggtttcatgt
     8521 atttactgac caaaagcaag tcggaaaggt aatgagtttc agaggtgaaa ctcggcattt
     8581 ctgccaggaa agatacgaag gaagtaagac tatcgtgcag cggatactgt cagcgataga
     8641 aaacggagag tacataacgg cctttatttc caaggggcaa gggcagcggt attaccacct
     8701 taaccatcat gatgacttca ttctaatgga gataagaaag cctgaaggta aagataattt
     8761 tcttcgcata catgttgtta cggcatatac gctggatgaa tgggggacgg tcaacaaagg
     8821 acggaatt
//
