LOCUS       NZ_CP137008             2953 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Citrobacter koseri strain K1219 plasmid pK1219-4, complete sequence.
ACCESSION   NZ_CP137008
VERSION     NZ_CP137008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37830357
            Assembly: GCF_033153635.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K1219 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: nuicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 06:04:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,687
            CDSs (total)                      :: 4,576
            Genes (coding)                    :: 4,524
            CDSs (with protein)               :: 4,524
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 20 of 52
            Pseudo Genes (incomplete)         :: 32 of 52
            Pseudo Genes (internal stop)      :: 9 of 52
            Pseudo Genes (multiple problems)  :: 9 of 52
            Pseudo Genes (short protein)      :: 1 of 52
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K1219"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK1219-4"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2019-02-11"
                     /collected_by="Jiayao Yao"
     gene            390..1364
                     /gene="mobV"
                     /locus_tag="R0291_RS23425"
                     /old_locus_tag="R0291_23425"
     CDS             390..1364
                     /gene="mobV"
                     /locus_tag="R0291_RS23425"
                     /old_locus_tag="R0291_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
     gene            complement(1391..1741)
                     /locus_tag="R0291_RS23430"
                     /old_locus_tag="R0291_23430"
     CDS             complement(1391..1741)
                     /locus_tag="R0291_RS23430"
                     /old_locus_tag="R0291_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(1738..2289)
                     /locus_tag="R0291_RS23435"
                     /old_locus_tag="R0291_23435"
     CDS             complement(1738..2289)
                     /locus_tag="R0291_RS23435"
                     /old_locus_tag="R0291_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
ORIGIN
        1 agggcagggt caagggcgcg cccgcaggcc gcagcccgga gcgaagcgca gggaccgggc
       61 gaggacgcgg gcgaagccga acccttgacg ctgaccgcag cgccccagcc tgtgcgagtg
      121 ggggcaggga cgaagggcga agcccgccac ccctgccccc cgagcctccc ggcggcgagg
      181 gtggggagtc cagaggggca aagccccttt gggcaaggcc gaaggccgcc gcaggagcaa
      241 gcccgaaggg cgctgcaacc gccgcaggcg ggcacgactt tgcttcgcaa agtctagtga
      301 gtacactatc atctgatagt ggcccgccgg aggcaccgcc ttgcgctgcc ccgactggcc
      361 ggattcggac accaagggga ggggcaggca tggcggccta cgcgatcatg cgatgcaaga
      421 agctggcgac gatgggcagc gtggcggcgg cgttgcagca ctgctaccgc gagcgcgaaa
      481 cgcccaacgc tgacgccgaa cgcacgccgg acaacgagca ccgggcggcc acctcaaccg
      541 atgcggcgat ggggcggctg cgcgagttgc tgccggagaa gcggcgcaag gacgcggtgc
      601 tggcggtcga atacgtgatg acggccagcc cggaatggtg ggccaaggcc acgccccagc
      661 agcaggccgc gttcttcgac caggcgcagg gctggctggc ggccaaatac ggcgcggatc
      721 gcatcgtgac ggccagcatc caccgcgacg aggccacccc gcacctgtcg gccttcgtcg
      781 tgccgctgac gcaggacggg cggctgtcgg ccaaggagtt catcggtggc cgcgacaaga
      841 tgcgggccga ccagaccagc tttgccgagg cggtgaggga tctcgggctg gaacgcggca
      901 tcgagggcag ccgggccacc catcagcgcg tccagagcta ctacggcgcc atcgagcgcc
      961 agcccgggca cgccaccatc acgccgcagg ccatcgagcc gcgggtgcta cgcaagggga
     1021 ttttctcgaa ggacgtggag acgccggagg ccgtggccgc ccgcgtgacc gctgccgtca
     1081 gggaagggta tggcccgacc gtcgcagcgg ccgcaggagc gcgtcaggag cgcgagaagg
     1141 cccggcaggc acaggagaca gcccggagcc tgcgtgaccg cctgaagccc gttctggacg
     1201 ctctggggcc gcttaatcgc gatatgcagg ccaaggcggc ccagatcatc aaggccgtgg
     1261 gcgagaagct gctcgccgaa cagcgcgagg cgacacgcca gcggcaggcc caacggcttc
     1321 gcagtcgcgg ccgggatcgc gacgacggcg ggctgtctcg ttgaccctac aggtggccga
     1381 cggaaggggt ttatggcttg cagctactga ggacggtttg ctccttcccg ccatcgtggg
     1441 tcgaaaggtt catgtcgttg cccttacccc acagttcgta ggtgtagttt ttgctagcgg
     1501 agatgtagcg cactcctgat gcgctcttgg cgcgtttcag tgggacaagc tcatccattt
     1561 gcagcatcac ggcatagtct ccgacgtaca cgacatccaa cgtctttccg ccttcgcaag
     1621 cgtacttggt ctggatcatc tcatccgctg ctgaagcaag attcggagcg attgccgcca
     1681 tcatggcagc aagagcgaac gtttttttca taaagatttt ctccttcgtt tcgttgctca
     1741 ttggggagtg ctttcaaaca tgtcaatggt gttcgggtcg cggtcgtcgt tctgcttctc
     1801 gccgctgaac atcgacatga tccggcggcg gatatcgcca tccttgttga ccaaggtttt
     1861 ggtcacgaaa gctgcgtaat ccttggcgtt gtcgtagccc ttccagacct cgtgaggatc
     1921 cagcgcatag gcgcaaacct tgccagcaac agggatgcgg acaagcgcgc gattttcttc
     1981 caggtaagac aaccaacggc tgattgtccg cctgttgacg ttcatcttgt ctgccaaaaa
     2041 ctgctgatcg cagaccaccg cgccgcaggt tgggtcgata ttttcagcaa agaatgaata
     2101 caggccgaag gccccagcat tgcctttagc aagctcaatg atgcgcttcc aacccaaggg
     2161 atacacctgc gtgaacccct tgttcttccg cgcctcttcg cgctcctgag cctctttctc
     2221 gtccagcatg gccagctctc gctggcggat acgacgctgg gcgtcggtcg ccaccgtgat
     2281 ctgctgcatg gggttcctcc aaagtcggga aatttgtccg ctcattgcgc catttcgagt
     2341 cgcaatgata cgacaaatta tgtctcacgt cgagacagtt tgcgtctcaa ccttgctacc
     2401 tgttgagaca tccatgcatg tctcaacgtt aggacaccct atgtgtctca atgcatttct
     2461 acaaatcctt ataaatcaat aacttagcat ttcccccttt ctcctctaag gggctgccgc
     2521 cccgccggcg gccgctacgc ggccttggca ccccgctgac ggcccctgcg gggcctctgg
     2581 aaaaggccca cccggccacc ggccgggtga cgttgtttgg ggggcctccg gcggccgctg
     2641 cgcagccttg gggcggtggg cgctgccccc cgccaccccc cgcgttcgcc acgtggcagg
     2701 ggtgcagggg ctacgggcct gcggcccttc actcctgcac ccctaccggg gagcgtgggg
     2761 tcgttccagg cgcgtcaagg gccgctgcgc tctccggggg tggcccgtgt cctcgccctg
     2821 cgggctgcgg gccgcgccag ccaccccctg cgcccttgac ccgccttcca ctccggcgct
     2881 gtcggaaatt tcctacccgg ccatgaggcg atttccgaca cgccagcggg ccactggctg
     2941 acgccttcag cgg
//
