LOCUS       NZ_CP137004            10060 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Citrobacter koseri strain K1219 plasmid pK1219-3, complete sequence.
ACCESSION   NZ_CP137004
VERSION     NZ_CP137004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37830357
            Assembly: GCF_033153635.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K1219 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: nuicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 06:04:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,687
            CDSs (total)                      :: 4,576
            Genes (coding)                    :: 4,524
            CDSs (with protein)               :: 4,524
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 20 of 52
            Pseudo Genes (incomplete)         :: 32 of 52
            Pseudo Genes (internal stop)      :: 9 of 52
            Pseudo Genes (multiple problems)  :: 9 of 52
            Pseudo Genes (short protein)      :: 1 of 52
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP137004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K1219"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK1219-3"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2019-02-11"
                     /collected_by="Jiayao Yao"
     gene            join(9415..10060,1..1040)
                     /locus_tag="R0291_RS00005"
                     /old_locus_tag="R0291_00005"
     CDS             join(9415..10060,1..1040)
                     /locus_tag="R0291_RS00005"
                     /old_locus_tag="R0291_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1050..1307
                     /locus_tag="R0291_RS00010"
                     /old_locus_tag="R0291_00010"
     CDS             1050..1307
                     /locus_tag="R0291_RS00010"
                     /old_locus_tag="R0291_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            1392..1541
                     /locus_tag="R0291_RS00015"
                     /old_locus_tag="R0291_00015"
     CDS             1392..1541
                     /locus_tag="R0291_RS00015"
                     /old_locus_tag="R0291_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3025..4989
                     /locus_tag="R0291_RS00020"
                     /old_locus_tag="R0291_00020"
     CDS             3025..4989
                     /locus_tag="R0291_RS00020"
                     /old_locus_tag="R0291_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4989..5720
                     /gene="mobC"
                     /locus_tag="R0291_RS00025"
                     /old_locus_tag="R0291_00025"
     CDS             4989..5720
                     /gene="mobC"
                     /locus_tag="R0291_RS00025"
                     /old_locus_tag="R0291_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5727..6257
                     /locus_tag="R0291_RS00030"
                     /old_locus_tag="R0291_00030"
     CDS             5727..6257
                     /locus_tag="R0291_RS00030"
                     /old_locus_tag="R0291_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6284..6463)
                     /locus_tag="R0291_RS00035"
                     /old_locus_tag="R0291_00035"
     CDS             complement(6284..6463)
                     /locus_tag="R0291_RS00035"
                     /old_locus_tag="R0291_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6489..6917)
                     /locus_tag="R0291_RS00040"
                     /old_locus_tag="R0291_00040"
     CDS             complement(6489..6917)
                     /locus_tag="R0291_RS00040"
                     /old_locus_tag="R0291_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7081..7395)
                     /locus_tag="R0291_RS00045"
                     /old_locus_tag="R0291_00045"
     CDS             complement(7081..7395)
                     /locus_tag="R0291_RS00045"
                     /old_locus_tag="R0291_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7383..7661)
                     /locus_tag="R0291_RS00050"
                     /old_locus_tag="R0291_00050"
     CDS             complement(7383..7661)
                     /locus_tag="R0291_RS00050"
                     /old_locus_tag="R0291_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7836..8201)
                     /locus_tag="R0291_RS00055"
                     /old_locus_tag="R0291_00055"
     CDS             complement(7836..8201)
                     /locus_tag="R0291_RS00055"
                     /old_locus_tag="R0291_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8198..8569)
                     /gene="tolA"
                     /locus_tag="R0291_RS00060"
                     /old_locus_tag="R0291_00060"
     CDS             complement(8198..8569)
                     /gene="tolA"
                     /locus_tag="R0291_RS00060"
                     /old_locus_tag="R0291_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8843..9088)
                     /locus_tag="R0291_RS00065"
                     /old_locus_tag="R0291_00065"
     CDS             complement(8843..9088)
                     /locus_tag="R0291_RS00065"
                     /old_locus_tag="R0291_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1645..2223)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4990..5717
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg attccgggtc
       61 tcccgtctct gcaggtgagc gtaccgaaag gtgttccgac agcgaaagcc ccgccaaaag
      121 gcattgttgc tgaaaaaggt gattcacgtc cggctggttt tacagccggt ggtaactccc
      181 gtgaggccgt tattcgtttc ccgaaagaga ccggacagaa gccggtttat gtgtcggtga
      241 cagatgttct taccccggca caggtaaaac agcgtcagga ggaagaaaag cgtcgccagc
      301 aggcatggga cgccgctcat ccggaagagg ggctgaaaag agactatgat aaagcgaaag
      361 ctgagctgga tgccgaagat aaaaatattg cgaccttaaa cagccgcatt gcatcgacag
      421 agaaggcgct ccccggtgca agggctgctg ttcaggaagc cgataaaaag gtaaaagagg
      481 cagaggcaaa taaagatgat tttgtgactt ataacccgcc tcatgaatat ggctccgggt
      541 ggcaggatca ggttcgctat cttgataagg atattcagaa tcagaatgag aaattaaaag
      601 cggctcaggc atctttaaac gcaatgaatg aatccttatc cagagataag gctgcactta
      661 ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca gaaaataagt
      721 taaatgagga aaagaacaag cctcgcaagg ggactaaaga ctacggccat gattatcatc
      781 cagtacctaa aacagaagac ataaagggac ttggagagtt aaagcgaggt gatcctaaaa
      841 cacctaaaca aggtggtggt ggtaagcggg cccgatggta tggcgataaa aaacgtaaaa
      901 tttatgaatg ggattcccag cacggtgagc ttgaagggta tcgtgccagt gatggcgaac
      961 acctaggcgc atttgatccc aaaacgggta agcaggttaa agggccagat ccaaaacgga
     1021 acattaaaaa atatctttga gaggtaaata tggggcttaa attacgtttg acatggtttg
     1081 ataagaaaac tgaagagttt aaaggggaag agtattctaa agactttggt gacgatggtt
     1141 ctgtcattga aagcttggga atgcctttaa aggataatat taataatggt gagtttgatt
     1201 tgaagaaaga atgggttcct ttactacagc cattttttaa aaatcaaata aaacctgatg
     1261 tggataattg ttatatagct tttgattatc gggacggtaa ctggtaacaa aaaattatca
     1321 aggatgattt gaaatactga cttccggtgt tcagggatga atgccggagt ctttaaatac
     1381 agggaaagta tatgaaaaag gtaaagacta tttttctttt tatattgatt gttgctggct
     1441 ttcttctggc cgcatgtcag gcaaattata tccgggatgt tcagggcgga acggtggcac
     1501 catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc
     1561 ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg
     1621 aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt
     1681 cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca
     1741 agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc
     1801 tgccagtggc gttttgcgtg ctttcccggg ttggactcaa gtgaacagtt accggataag
     1861 gcgcagcagt cgggctgaac ggggagttct tgcttacagc ccagcttgga gcgaacgacc
     1921 tacaccgagc cgagatacca gtgtgtgagc tatgagaaag cgccacactt cccgcaaggg
     1981 agaacggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg caagagggag
     2041 cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga
     2101 ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaac
     2161 tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc cgttttgcct
     2221 cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg tgtccgcagc
     2281 gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg cgcaatttcc
     2341 ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc ctgattaagt
     2401 ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt tttcgctgaa
     2461 cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg caagtcatcc
     2521 ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct ggacgccacc
     2581 gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc cacgccagaa
     2641 attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac ggtgctggac
     2701 gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg ctcgctatgg
     2761 cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg ccttttcctc
     2821 tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc atggggcgga
     2881 aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc
     2941 catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg ccggtaaacc
     3001 cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg gcagtggcat
     3061 atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg ggagtattac
     3121 agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga actctgggcc
     3181 ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc cgatcgctat
     3241 aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga ttcacccggg
     3301 cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac cggcgctgcc
     3361 ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc
     3421 gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc ggctaacctg
     3481 ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc gcggctgctg
     3541 gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa cgcgaaactg
     3601 aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt ccgccacctg
     3661 catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga gcaggcccgg
     3721 aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct ggtccctcgt
     3781 gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg caagagcgcg
     3841 atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt ggtgtacttc
     3901 gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc ggacttcacc
     3961 ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag
     4021 tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg cgacgtggcg
     4081 gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gttcccgcag
     4141 ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc cctgaaaaag
     4201 gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt cctgcagacg
     4261 gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta cgtcatcggc
     4321 tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa
     4381 atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat gctcgatgag
     4441 attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg cagccgggac
     4501 tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg gcaggatctg
     4561 cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttctatcgc
     4621 gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat ccgggtggac
     4681 gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag cggcgacagc
     4741 ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca cctgcctgac
     4801 gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag cccgctgcgg
     4861 gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac ggacccgctg
     4921 gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga ttttgcgggg
     4981 ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag gcgcggaacg
     5041 cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc acggcggagc
     5101 tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc ctggacagtc
     5161 tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt caggtcagcg
     5221 tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag ccgctgacgg
     5281 acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac cggctggccg
     5341 tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg tacctgcacc
     5401 gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac ggcagaacgg
     5461 tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa gtggtgtccg
     5521 ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc tgccctgacc
     5581 ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc gtgaatccac
     5641 agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc gcgtgcttta
     5701 aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt gaggacgtgg
     5761 aagacaaaga cggcgagcgc tgcattatgc caaccagcga gcatctggaa gacatggtgg
     5821 tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg
     5881 cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa aaagccacgt
     5941 actttttcac cggtgaaaat ccgcaatggg aacgggacaa acccacagga atgaagctgt
     6001 gtgattacct ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg caggtggttg
     6061 atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgaggtggtc agcgagcggc
     6121 acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa tataacagcc
     6181 gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc cggtggtggc
     6241 cgttcggcaa aaaataacgc cagcaccggc atggccggtg ctgttattct tccccgagct
     6301 tatcccgcat cacctgccag agtgtttccg catcctcgtg aagccgttcg cacatgtcgg
     6361 ccggggtatc catgtccagc tcgttcagct tttccagcaa cagcagcgac tgatccttaa
     6421 tgaatttagc catattgagg gcagtttttt cctgcttcgt catgttctcc attcccttcg
     6481 gtaagttctc agctaaacca gccggaccag aagcgttttt tcttctgtgt cagttctgtt
     6541 gtcaatctgt ccacttcggc ctggagtttc cggcgctcat catcgtgctg gcggcgttct
     6601 tcttcccggg atgttttatc ttccagcatc agggtgacag cctgccggag atcggccagt
     6661 tccttttgca ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt ttcacggtgt
     6721 gaaatgccgg gaatttgttt ctcagggata ttcacatttc cgtatacacg gatcagttct
     6781 gacacatcca cgacagggtt gtttttcccg tcacgtgaca cggtaacctt tccctgtttt
     6841 acatgattgt atagcgttct tctggtaatg cccgcggctt gtgctgcctg tgaaaggttg
     6901 agcaatgttt tcgccactgt atacccctga ttttatcggg tgtgaaaggt gtgtaatttc
     6961 tcacggtaaa tgtatcgctg aattttaccc gtttttccgt tcaggatgtg gttttctgac
     7021 gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg tcatcggtta
     7081 ttagaggctg tctgcccccg gatacccgga ttctttctga tgcctgacgg tgacgatcac
     7141 aatctggtcc atttcccggt cgtggcggta gagcatcaca tagccactgt cgccaaaccc
     7201 gatcaccagt tccttatatt ccagcggcag aaacggcacc gggcggccaa tgtctggcaa
     7261 tgttttcagt tgctggatag cccggacgat cacctcacct ccttttctgg ctgccagccg
     7321 atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca gcgcggaaat
     7381 cgttacctgt ggcatggcgg agcatcctgc tcgttctcag ttccccaggt acttatccag
     7441 gcttccgctt cttccgcagt aaggtgcaga cccgtttcct gatagtgctg ccatgcggcc
     7501 tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt cgatgtattc ggtgattgcc
     7561 tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt cagccggtcc
     7621 ttaagttctt catcgagcct gatggatgtt gctgatgaca taaaagcccc tttgtagtca
     7681 tatgtattac acatgactac tttaatccgt ggcggcgcta tttgccagaa atacggcttt
     7741 tgctgcccat ttttcagctt cgcttccact aatcagggct gctacaggct cactcatcat
     7801 ccaggactat tgcagcctgc gggttaaagg acctgttact ggcggcaacc atcatcattg
     7861 agcttttggg tggtgactgg tcgcggatcc tgtaagccgt catttttcag actgaattca
     7921 aaatttataa cctgtcgcat tgccgggtgc cctgcgggat atagccagtg cctcattgcc
     7981 gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc cactcgtcca
     8041 tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt
     8101 accgggtagg ttggctgcag tgtggttacg ggttttagtg gtatcgcatg taccggagtg
     8161 aggaacatgg ccgccagcac tgtcagagta aaagctttca tggtctgaaa tccagagggg
     8221 cgtttttgaa tatttgccag gtattttcat ccggtgccgg cggaattttg gcgtgggtaa
     8281 cggctgatat tgctgcttta cagagtttcg gatctcctcc ttcagcaatg gcgctgttca
     8341 gagtgccatc acgttgtatg gatatccgca cggtgcacat ttttcccgca taggcctctg
     8401 gttcaaacat ctgcatttca acagcggtct tgatggccgc agcgtagcta ctgattttag
     8461 atgacttatc cagagtataa attttcggcg gctgggtggt ggaatgctta caggccgtca
     8521 ggagtaaggg gctgcacaat gccagcagga taaagggtat taaacgcatc gggttgatga
     8581 cctgtcagta aaaaatgtta catgtatatt tgtcactctt tacggccacg ttcatttaca
     8641 taaacacggc ttatcgtatc gtttttacac caaacggccc gccatgcagt tgcgtgcata
     8701 ggcatgcagc gggttacaat ttaccgggcg cgtttccggc ggttttccgg gtggtttgtt
     8761 gcctgtttta tcccgtagcc gccggaaacg ccctcagacc gtctgagcgg tgcgggcaat
     8821 gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg ctgccaggtg aaggtggttt
     8881 ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg cctggcttca cgttcgtcct
     8941 gaatggcacg aagcaccctt tcccgcacct gttgttttac cgcctttggc agcgttttgt
     9001 tccctttcag gcccatggcc agtgcctgag cgacaaaggc gtccgccagg cgcttttctt
     9061 cagcgggagt cagtttcagg tgcgtcatag tggcctccgg gcgtctcagg tttcccgaac
     9121 agtttaaccg caggtcctct cctcatgcca gttagctttc agatgtgtct ataccatctg
     9181 aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt tgccatgaga aattttcggg
     9241 aaaagacgcg atctgcttac aggtggctcc gggcgctttt ttgtgcctga agatgagaac
     9301 taatctcata tattgacacc tgaaaacggg aggagtaagg taataattat actgtgtata
     9361 tgtacagtat gttggcttta atttattgtt ttaaatgtca aagaggaaaa cggtatgagt
     9421 ggtggagacg gtcgaggtcc gggtaattca ggtctgggac ataatggtgg tcaggccagt
     9481 gggaatgtga acggtacgtc tggtaaaggt ggcccttcat caggtggtgg tacggatcca
     9541 aacagcgggc cgggctgggg tacgacgcat acgccgaatg gagatattca taactataat
     9601 ccgggggagt ttggtaacgg cgggagtaaa cccggtggta atggtggtaa cagcggcaat
     9661 catagcggta gctctggtgg cggacagtct tcggccactg cgatggcctt cggtctgcct
     9721 gccctggcta ctccgggggc tgaaggactg gctttatcag tttccggcga tgcgttgtcg
     9781 gccgccgttg ctgatgtgct ggctgccctg aaggggccgt ttaagtttgg tctgtggggg
     9841 attgcgatct acggtgtgct gccttctgag attgcaaaag atgatccgaa aatgatgtca
     9901 aaaattatga cgtcattacc ggccgatgcg gtgacggaga ctccggcaag tactttacca
     9961 ctggaccagg cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg
    10021 cagcatattg cggttgtcgc aggtcggcca atgagtgtcc
//
