LOCUS       NZ_CP136852             5596 bp    DNA     circular CON 13-NOV-2023
DEFINITION  Klebsiella pneumoniae strain L2329 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP136852
VERSION     NZ_CP136852.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37688351
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Fu,H.
  TITLE     K. pneumoniae L2329,complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Fu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2023) The First Affiliated Hospital of Zhejiang
            University School of Medicine, Zhejiang University, Qingchun Road,
            Hangzhou, Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/13/2023 12:58:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136852.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L2329"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-07-16"
     gene            156..284
                     /locus_tag="RW110_RS00005"
     CDS             156..284
                     /locus_tag="RW110_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(660..872)
                     /locus_tag="RW110_RS00010"
                     /old_locus_tag="RW110_30370"
     CDS             complement(660..872)
                     /locus_tag="RW110_RS00010"
                     /old_locus_tag="RW110_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(879..1388)
                     /locus_tag="RW110_RS00015"
                     /old_locus_tag="RW110_30375"
     CDS             complement(879..1388)
                     /locus_tag="RW110_RS00015"
                     /old_locus_tag="RW110_30375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1423..2127)
                     /locus_tag="RW110_RS00020"
                     /old_locus_tag="RW110_30380"
                     /pseudo=""
     CDS             complement(<1423..2127)
                     /locus_tag="RW110_RS00020"
                     /old_locus_tag="RW110_30380"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2066..2410)
                     /locus_tag="RW110_RS00025"
                     /old_locus_tag="RW110_30385"
     CDS             complement(2066..2410)
                     /locus_tag="RW110_RS00025"
                     /old_locus_tag="RW110_30385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2400..2576)
                     /locus_tag="RW110_RS00030"
                     /old_locus_tag="RW110_30390"
     CDS             complement(2400..2576)
                     /locus_tag="RW110_RS00030"
                     /old_locus_tag="RW110_30390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3216..3374
                     /locus_tag="RW110_RS00035"
                     /old_locus_tag="RW110_30395"
     CDS             3216..3374
                     /locus_tag="RW110_RS00035"
                     /old_locus_tag="RW110_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3732..4058
                     /locus_tag="RW110_RS00040"
                     /old_locus_tag="RW110_30400"
     CDS             3732..4058
                     /locus_tag="RW110_RS00040"
                     /old_locus_tag="RW110_30400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4084..4629
                     /locus_tag="RW110_RS00045"
                     /old_locus_tag="RW110_30405"
     CDS             4084..4629
                     /locus_tag="RW110_RS00045"
                     /old_locus_tag="RW110_30405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            4695..5045
                     /locus_tag="RW110_RS00050"
                     /old_locus_tag="RW110_30410"
     CDS             4695..5045
                     /locus_tag="RW110_RS00050"
                     /old_locus_tag="RW110_30410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5115..5381)
                     /locus_tag="RW110_RS00055"
                     /old_locus_tag="RW110_30415"
     CDS             complement(5115..5381)
                     /locus_tag="RW110_RS00055"
                     /old_locus_tag="RW110_30415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5365..5589)
                     /locus_tag="RW110_RS00060"
                     /old_locus_tag="RW110_30420"
     CDS             complement(5365..5589)
                     /locus_tag="RW110_RS00060"
                     /old_locus_tag="RW110_30420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     Typing          complement(2663..2739)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          2928..3325
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(545..2076)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcccgaacac catttgctat acatgtatat caacagtacc acaatcaacc taaaagaaca
       61 aaaaataatc ttgttagccc ctcttgataa tccgtaacgc ggtcaatata tctagcttga
      121 gggctttcct cttaactatt gaaaggataa cgattatggg tcttaagccg ggtccgaaac
      181 gaattgccga ttcaacaggg gaacctgata aacgccagcg tgacaataaa aaaacgccgg
      241 gaaatactga caaattgaaa cccagcaaat cttcaaaaaa ataagtcagt agttaaaagc
      301 cctccggggc ttttttgtat ctgccgttca cgctgattaa acataacacc cgttgcccgc
      361 accgccccgg acgggttcgc cgcgaacggg gtggcagtca ggtaaaaccg gcaacaaacc
      421 acccgataaa ccgccccgtt cgcgacagat aaattttacc tggctgcata gccatgcagc
      481 caggtgaatg acatccggtt tcagcgtaaa tgcccgatga gaaccctgtt ttcctgcatt
      541 tcagcccagc gacggaccgt catagcttcg ctgccgggaa cgcgcttccg tcagttcctt
      601 ttcgtgctgc cgttgctcgt gcgcccgttc tgcggccacg gccatctcat gacgctgcac
      661 taccggctca aacgctgcag ttgcccgctc aagcgttcca cctgctgact caagcgcgtc
      721 acgcgctcgc tcagcgccgc gttctcccgt tgcataagcc ctgacatttt ccgccattcc
      781 ccgaaggcgc tctcccactc gtccagcctt ttcgagtagt cctgctgtag ctgctctaat
      841 gcgctcagca actgtttttc cagctcggtc atcggcactt actccttcac cagtctcagc
      901 gcgttctgct tcgaccgccc gttcccgacc gtccagctct gcgtcctgtc tacggccgtc
      961 cccttcggca gcaccagaaa acgtccgttg ctgtcttcca ggtacgtcac gccccaggtc
     1021 ttcgcgttca gcttcgacag cgctgcgttc tgccggtcga tttcggccag atttgacgct
     1081 atcaagctcc cctgatacca caacaccccg ctcaggctgg cgaacagcag tcctgacacc
     1141 atcgtgatgg tcagccatgt ccgcccgacc atacgcatca cgctcaggcg gttcgactgc
     1201 atggccgcgc tcataccctg ctcgtgggcg cggatggcgg cgctgattct cttcccgctt
     1261 tcgctcagtt ccgctctgac agacttctcg tgctcgctga atgcggcttt cagcatttcg
     1321 ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga
     1381 ctgttcatag atggctccct taagtcggat gtttcgccct ccgtccgggt cggcaatgct
     1441 gatgctgctc tttgtggtgc gcacgacctc aaaacctgcg ctttccagcg cctcagtgac
     1501 gtcctgacgc gttttaagct cccctgacga ggcgagggcc agtaagcccc gcgtaatagc
     1561 ctctgcggct tcctgcttcg ttttgggtaa cgaagatggc gtcaccagcg cgcgccggtt
     1621 ctccggtgcg ttcgggtcgt gcagccccag tcgcccgttc accacggtct gccacgcatc
     1681 gatgcgcgga cggtctgccc ggtcgtagta cggctggaga cgcctgccgg tcagcagttc
     1741 cgtgttcggt accagaaaat tcagttccag ccgccccttg tcccggtgtt caacccacag
     1801 cacgctgtac tggtctttat cgagtccggg catcagaacc cgctcgaagc tcgccatcag
     1861 cttttcacgc tggccgggcg gtaaatcctg ttcagcaaaa gacaggacgc cggaggtgta
     1921 ctttttggcg tagggcgagg cgtcgataag ctcccggacc tcctccggct ttccctgcag
     1981 gacgatggcc ccgtcgcgct ggcggtcttt ccccagcaga taatccaccg gcccggcacc
     2041 gccgcctcgc cctcgcggat gaaacttaac gatcatcgtc tgcccccttt tccagcacgg
     2101 catgccgcag ccgctcgagt tcggcatcaa tggccatcag cgcggccacc acctgaaccc
     2161 ggtcagcgcc tgaccactgg ccgccgttaa ttttccgggc gatctggttg aggttgttcc
     2221 ccatcccggc aagctggcgc agcaaaaccg gatcgatgga cggcagccgc aacgaccgcg
     2281 ccgggcgcgt atcgagacag gtttcccgca tccacaccgc cagttgcctt ccattgcagc
     2341 gctccagcag ctgctggtac tccccatcgg tcacccggat acctaaaaaa cgggtacgct
     2401 taaccgccat cctctcctcc gatatccgca aaatgtgacc gggctgaacg ctcaagctct
     2461 gccatctgct cccggcggta atcttcctcc tgtgcctgac gcatcagatt tgccatcact
     2521 gccggatgcc aggatctttt ttttacgacg agcggcttct tcttcggctt ctgcattatc
     2581 cctccttcgc aaacgccgga cggcacggaa aagttttccg ggtcgctccg gcgttggaca
     2641 agcgccagcg cgtcagtggt gttagcgggt ttcggggcgc agccctgaac cagtcacagc
     2701 gtttacgctg tgtatactgg cttactatgt tggcatgaga gcacgattca tgaaagtgcc
     2761 tcatgtggca ggtgtgatat gtggctcagg agcgtccgca ataccatgca cggacagatg
     2821 aaatgacgcc tcctcgctca ctcggtagct gcgctcctgc cgttcggctg cggcgggcgt
     2881 gaccgactca cggataacgg gataaagcaa aggcgctcaa atcagaaagg gccggagaaa
     2941 aggatgcggc gttgccgttt ttccataggc tccgcctccc ctgacgagca tcacagaaat
     3001 ggacgctcaa gtcagaggag gacgaaaccc gacaggactt aaagatgccg gtgatttctc
     3061 tccggcggct ccctcgtgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc
     3121 cgcctttttc cctttcggga agcgtggcgc tttctcatag ctcacgctgc tggtatctcg
     3181 gctcggttta ggtcgttcgc tccaagctgg gctgtatgca cgaacccccc gttcagcccg
     3241 actgctgcgc cctttccggt aactatcatc ttgagtccaa cccggacgga cacgcaaaaa
     3301 cgccactggc agcagccatt ggtaactgga tatatacgag agagatattg agaggtcttg
     3361 aagtgggtgc ctaactgcgg ctacactgaa agaacagttt tggtatctgc ggtctcgtat
     3421 acccagttac ccggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc
     3481 caggcggttt tttcgtttac agtgcaagag attacgcgca gaaaaaaagg atctcaagaa
     3541 gatcctttaa atgatcgtca gttctgttat ccccggagct ccatccatcc cacggaaaaa
     3601 cacccgccac tgatgccctc agcgcactgc acatcatgta caaaccactc cctccctcct
     3661 tttttattgc gtttcttaat gcgcaatggt agctttgtgc gcaacaaaaa acgcaatagc
     3721 aagggggggt aatgagagaa agaacaaata tcatgctaga tataggatta aagcaatcct
     3781 tacaacggct ttcgaataaa accaaaatga gtctaagtga tcttattaac cagagattat
     3841 caggttcact ttcagttgaa gaacgcattg gcgaaaagcc tgaatggatg agaaaccttg
     3901 atccctatct cgccaatcac ttttcaaata ttgagttacc gctcccttca ccattaaaag
     3961 aataccaaga aaaaatggat gagatagatt caattattaa tgacatgatg gttctaaagg
     4021 aatatttcag agctaagttt tacgacgaaa taaaataggc acgctatacg gaggcggaga
     4081 attatgtttt ttaaacggta tttaacggag aaggaaatat acaaggcagc actcccaatt
     4141 gttaggcttc aagggagagt taacacttca ttacgttttt tatccaaata ctcagatgta
     4201 aacaaaaatg gattaattgc attgcatctt aatttattac attttatctc atacaacata
     4261 cacggtgtag agtctctgaa aatcactcaa gcaggcataa acaaagctgt acaacaaagc
     4321 cctcacgcag atgaaataaa aaatattgcg caggcaatag gacgcaaagg acctgagtac
     4381 atcgaaggat taattagaaa cccatggggg aagaaaaatg ataatccact aatatttata
     4441 atgagcattg aagaaagtat aaaagaaacc cttaactcag gttatatgtt atatagagta
     4501 gctcccataa ctgagggtga agcagctgct ttcatggatg ttcttaaaca attattatta
     4561 tttgaggata attttcgcct ttttggtaga gacttggttt ataaaataga aaaattaaaa
     4621 atcagataac aaagtatggt gaacgaaaaa tgaaagagag gatagtgaaa tgtgtaatac
     4681 caagattctc aactatgtat tggttaattg cgttcctagc atttatattc cccggtcgat
     4741 cttttgctga cggttacacc gcttactacc gatgtaatgg tactcaggaa gcatctctta
     4801 ctgttaaaga taacaaacta agtatggttg taggtctttc taagtttaat aggcaactgg
     4861 atacttatag attcaaagaa aagggaatcg aagtagtaat gcagagagct gcttctgatg
     4921 ataactctga ggccataatg tcttttatta tgcctgacgt taacaatatg accttattag
     4981 ttaaaaataa cggctacatt atcgttaaga caacctgctg gttaactaaa accgacaaac
     5041 agtagaacac gtagttagtc gagtatttac cataatacct gttgtcggca tcaaaaaagc
     5101 ccgcatagcg ggcttcagtc gtaaacttcg cggcgatgtc caatcgtggc ggcaaggata
     5161 acaagctcgt catctcggat ctcacacaga acgcgataat ctcccacccg atagcgccag
     5221 aactcgccca gctcaccttt gagcggcttc cctgactggc gaggatcggc agcaactgca
     5281 atgcgtaaac tcataaaatc cacaatccgt cgtgcgttct gtttgtccat cttgcgtaac
     5341 gatttgagcg cccgatcgga atagttaatc gtccagacca agtgacttcc tcacgtcttc
     5401 agacgaatgc actgcttcat caccacggcg aacgcgtgca gcagtttctg ctgaaagata
     5461 ataatcctcc aggtcttcca gatgcgccag tattgcttcc cgcgcataaa acgtctttgt
     5521 tctgccggtt tgcttcgcca gcgagtccag acgggactca atctcatcag ataaccggat
     5581 agcaagcata taaacc
//
