LOCUS       NZ_CP136851            11970 bp    DNA     circular CON 13-NOV-2023
DEFINITION  Klebsiella pneumoniae strain L2329 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP136851
VERSION     NZ_CP136851.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37688351
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Fu,H.
  TITLE     K. pneumoniae L2329,complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Fu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2023) The First Affiliated Hospital of Zhejiang
            University School of Medicine, Zhejiang University, Qingchun Road,
            Hangzhou, Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: unicycler v. 0.4.7
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 200.0x
            Sequencing Technology        :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 11/13/2023 13:02:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 14
            CDSs (total)                 :: 14
            Genes (coding)               :: 14
            CDSs (with protein)          :: 14
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136851.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L2329"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-07-16"
     gene            join(11333..11970,1..1327)
                     /locus_tag="RW110_RS00005"
                     /old_locus_tag="RW110_30300"
     CDS             join(11333..11970,1..1327)
                     /locus_tag="RW110_RS00005"
                     /old_locus_tag="RW110_30300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1327..2058
                     /gene="mobC"
                     /locus_tag="RW110_RS00010"
                     /old_locus_tag="RW110_30305"
     CDS             1327..2058
                     /gene="mobC"
                     /locus_tag="RW110_RS00010"
                     /old_locus_tag="RW110_30305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2065..2595
                     /locus_tag="RW110_RS00015"
                     /old_locus_tag="RW110_30310"
     CDS             2065..2595
                     /locus_tag="RW110_RS00015"
                     /old_locus_tag="RW110_30310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2622..2801)
                     /locus_tag="RW110_RS00020"
                     /old_locus_tag="RW110_30315"
     CDS             complement(2622..2801)
                     /locus_tag="RW110_RS00020"
                     /old_locus_tag="RW110_30315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2827..3255)
                     /locus_tag="RW110_RS00025"
                     /old_locus_tag="RW110_30320"
     CDS             complement(2827..3255)
                     /locus_tag="RW110_RS00025"
                     /old_locus_tag="RW110_30320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3419..3733)
                     /locus_tag="RW110_RS00030"
                     /old_locus_tag="RW110_30325"
     CDS             complement(3419..3733)
                     /locus_tag="RW110_RS00030"
                     /old_locus_tag="RW110_30325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3721..3999)
                     /locus_tag="RW110_RS00035"
                     /old_locus_tag="RW110_30330"
     CDS             complement(3721..3999)
                     /locus_tag="RW110_RS00035"
                     /old_locus_tag="RW110_30330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4174..4539)
                     /locus_tag="RW110_RS00040"
                     /old_locus_tag="RW110_30335"
     CDS             complement(4174..4539)
                     /locus_tag="RW110_RS00040"
                     /old_locus_tag="RW110_30335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4536..4907)
                     /gene="tolA"
                     /locus_tag="RW110_RS00045"
                     /old_locus_tag="RW110_30340"
     CDS             complement(4536..4907)
                     /gene="tolA"
                     /locus_tag="RW110_RS00045"
                     /old_locus_tag="RW110_30340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5181..5426)
                     /locus_tag="RW110_RS00050"
                     /old_locus_tag="RW110_30345"
     CDS             complement(5181..5426)
                     /locus_tag="RW110_RS00050"
                     /old_locus_tag="RW110_30345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6071..7558
                     /gene="ltrA"
                     /locus_tag="RW110_RS00055"
                     /old_locus_tag="RW110_30350"
     CDS             6071..7558
                     /gene="ltrA"
                     /locus_tag="RW110_RS00055"
                     /old_locus_tag="RW110_30350"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7663..9348
                     /locus_tag="RW110_RS00060"
                     /old_locus_tag="RW110_30355"
     CDS             7663..9348
                     /locus_tag="RW110_RS00060"
                     /old_locus_tag="RW110_30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9358..9615
                     /locus_tag="RW110_RS00065"
                     /old_locus_tag="RW110_30360"
     CDS             9358..9615
                     /locus_tag="RW110_RS00065"
                     /old_locus_tag="RW110_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9700..9849
                     /locus_tag="RW110_RS00070"
                     /old_locus_tag="RW110_30365"
     CDS             9700..9849
                     /locus_tag="RW110_RS00070"
                     /old_locus_tag="RW110_30365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2663..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9953..10531)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1328..2055
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tttcggggag ccggtaccgg ttaacgcgct ggccgatttt tccacggagc aggcccggaa
       61 acagcaggcg gtgtttctgg gtaaggatga gcagggccag ccggtgctgg tccctcgtga
      121 tacctggcgc aagaccaata ttcagatcct cggcctgccc gggagcggca agagcgcgat
      181 ggccaccaac gcgctcattc gctgcgtacg cgatttcggg gacgctgtgg tgtacttcga
      241 ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg cactgcccgg acttcaccct
      301 gctggatctg cggccgggga aaccggcgca gctgaatctg ttccgcgatc tcgaccagta
      361 cgccctgaag aatctgctgg ttgccgggtt taacctgagt gagaccggcg acgtggcgga
      421 ccactaccgc atcagcgagc agaaagcggc gaagctgatt gcggagcagt tcccgcaggg
      481 ggcaaacatt cagcaggtgc tggcggcggc gtacgcgttg ccggaggccc tgaaaaagga
      541 cgtgaagggg ctgatcacca agctggagaa cgtggccgac ctgagcgtcc tgcagacgga
      601 cagcggtatc gatgtggcgg ggataatcaa cggcggcggc tgcctgtacg tcatcggctc
      661 gatggacgat gaggcggtga tccgggtgca gaagatgctg tttgcccgct gcgcgcaaat
      721 catcattgcg cgggatgagt tccggaggtg gccgcacgcg agcatcatgc tcgatgagat
      781 taaatatctg ctgtcgaagt acgtgctgaa cgcgctgggg acgctgcgca gccgggactg
      841 caacctgctg ctcgcgcacc agtcgctggg cgacttcggt cagtgcgggc aggatctgcc
      901 cgctgatttt gtgaaaacca cggtactgga taacacgccg atccgctggt tctatcgcgc
      961 ggccagccag gagtccgcgc agtgggcggc gggccagacc ggggagatcc gggtggacgt
     1021 ggagcgccgc cgggccagcc gggaggcggg gaacgtggag cacatcagcg gcgacagctt
     1081 catccagaag gaggcgcggc cgctgtttga cgtgaacacc ctgcaacacc tgcctgacgg
     1141 gttcgcggtg atgaccgggc tgggcgtggc gcggctggcg ttcagcagcc cgctgcgggt
     1201 tgagcgccgg gaaatttcgc tgaaaacctt cccggtgctg gcgaaaacgg acccgctggc
     1261 ggagtacctg cctccggccg acggcggcgg accaaaagcg gatgatgatt ttgcggggct
     1321 ttactgatga cactggagca gtacaacgtc agccacgcga agcggcaggc gcggaacgcg
     1381 gagaaaacac ggctgaccct gcgctggctg cgggaagagc tgtgttccac ggcggagctg
     1441 gttgcgcgac ggctggggat tgcggcggta cagccggttt accgcttcct ggacagtctg
     1501 gtggcgaaag ggctgctggt ccgggcgaaa tacccggttg acggccgtca ggtcagcgtg
     1561 tgggggctga cgccgcacgg ggtggcgttc tcgttcgacg aggacgagcc gctgacggac
     1621 gttattccgt ttcagccgtc aagggtgtcg gcggcgcagc tgccgcaccg gctggccgtg
     1681 cagtccctgc gcctctccat ggagggccgg ggggccaccg gctggcggta cctgcaccgt
     1741 atggccctga aagggatgaa agtgccggac gcgctggcgg agctggacgg cagaacggtg
     1801 gcatttgagg tagagcgcac cgtcaagagc cgcaggcggt atcaggaagt ggtgtccggg
     1861 tatctgttca accgccgggc gaacggcatc gatgaaatct ggtacatctg ccctgaccgg
     1921 ccgacgcttg tccgggtaca gcgggcgatt ctggcggtgg atgaaatcgt gaatccacag
     1981 accggcgagg cccgcaaaac ggtggagctg gaccgggaga ggctgttcgc gtgctttaaa
     2041 tttatgacaa cggagtgagg gattatggcg ctgtacatgg tatttattga ggacgtggaa
     2101 gacaaagacg gcgagcgctg cattatgcca accagcgagc atctggaaga catggtggtg
     2161 ctcgcctggc agaccggggc gtatgttttc tgttttgtcg accgggccca gcatctggcg
     2221 atgatgaaat ccatcgggat gtatgacgag cggctcaaca gcgacgaaaa agccacgtac
     2281 tttttcaccg gtgaaaatcc gcaatgggaa cgggacaaac ccacaggaat gaagctgtgt
     2341 gattacctct atcgaaaggt ggagcggttc gccatcgaga acggtgtgca ggtggttgat
     2401 gccgggaagt gctcaccgga gcagattaac gcgatgattg aggtggtcag cgagcggcac
     2461 ggggaaagcg cggtgaacga gaagctgaaa gcggagctga tgcgggaata taacagccgc
     2521 tattttaacg tgccggtgcc tgaactgccg gaggacaaac ccgcccgccg gtggtggccg
     2581 ttcggcaaaa aataacgcca gcaccggcat ggccggtgct gttattcttc cccgagctta
     2641 tcccgcatca cctgccagag tgtttccgca tcctcgtgaa gccgttcgca catgtcggcc
     2701 ggggtatcca tgtccagctc gttcagcttt tccagcaaca gcagcgactg atccttaatg
     2761 aatttagcca tattgagggc agttttttcc tgcttcgtca tgttctccat tcccttcggt
     2821 aagttctcag ctaaaccagc cggaccagaa gcgttttttc ttctgtgtca gttctgttgt
     2881 caatctgtcc acttcggcct ggagtttccg gcgctcatca tcgtgctggc ggcgttcttc
     2941 ttcccgggat gttttatctt ccagcatcag ggtgacagcc tgccggagat cggccagttc
     3001 cttttgcatt gccagaagct gttcctgtgg gaaatttttt tgtgtatttt cacggtgtga
     3061 aatgccggga atttgtttct cagggatatt cacatttccg tatacacgga tcagttctga
     3121 cacatccacg acagggttgt ttttcccgtc acgtgacacg gtaacctttc cctgttttac
     3181 atgattgtat agcgttcttc tggtaatgcc cgcggcttgt gctgcctgtg aaaggttgag
     3241 caatgttttc gccactgtat acccctgatt ttatcgggtg tgaaaggtgt gtaatttctc
     3301 acggtaaatg tatcgctgaa ttttacccgt ttttccgttc aggatgtggt tttctgacgc
     3361 gctgcgcttg tcaaaccggc gacggccacg caatgaattg cgcccccgtc atcggttatt
     3421 agaggctgtc tgcccccgga tacccggatt ctttctgatg cctgacggtg acgatcacaa
     3481 tctggtccat ttcccggtcg tggcggtaga gcatcacata gccactgtcg ccaaacccga
     3541 tcaccagttc cttatattcc agcggcagaa acggcaccgg gcggccaatg tctggcaatg
     3601 ttttcagttg ctggatagcc cggacgatca cctcacctcc ttttctggct gccagccgat
     3661 ttttggtttt gagaaagtcc tgaaggcgtt gtaaatcgcg ctgtgccagc gcggaaatcg
     3721 ttacctgtgg catggcggag catcctgctc gttctcagtt ccccaggtac ttatccaggc
     3781 ttccgcttct tccgcagtaa ggtgcagacc cgtttcctga tagtgctgcc atgcggcctg
     3841 tccgtcccgc aggtactggc tgcgcttctc ttcccggtcg atgtattcgg tgattgcctc
     3901 cagcattagc gcgtgcgctg agcggtgccg gtcatcggcc agcgttttca gccggtcctt
     3961 aagttcttca tcgagcctga tggatgttgc tgatgacata aaagcccctt tgtagtcata
     4021 tgtattacac atgactactt taatccgtgg cggcgctatt tgccagaaat acggcttttg
     4081 ctgcccattt ttcagcttcg cttccactaa tcagggctgc tacaggctca ctcatcatcc
     4141 aggactattg cagcctgcgg gttaaaggac ctgttactgg cggcaaccat catcattgag
     4201 cttttgggtg gtgactggtc gcggatcctg taagccgtca tttttcagac tgaattcaaa
     4261 atttataacc tgtcgcattg ccgggtgccc tgcgggatat agccagtgcc tcattgccgt
     4321 cagcgtttcc cgaaaaaaaa ggtgacagcc ggtaagcgac acgttgtcca ctcgtccatc
     4381 gtccagaata tcaaacgtgg cggtcacctg accttcgatg cgttttgctg cggcctgtac
     4441 cgggtaggtt ggctgcagtg tggttacggg ttttagtggt atcgcatgta ccggagtgag
     4501 gaacatggcc gccagcactg tcagagtaaa agctttcatg gtctgaaatc cagaggggcg
     4561 tttttgaata tttgccaggt attttcatcc ggtgccggcg gaattttggc gtgggtaacg
     4621 gctgatattg ctgctttaca gagtttcgga tctcctcctt cagcaatggc gctgttcaga
     4681 gtgccatcac gttgtatgga tatccgcacg gtgcacattt ttcccgcata ggcctctggt
     4741 tcaaacatct gcatttcaac agcggtcttg atggccgcag cgtagctact gattttagat
     4801 gacttatcca gagtataaat tttcggcggc tgggtggtgg aatgcttaca ggccgtcagg
     4861 agtaaggggc tgcacaatgc cagcaggata aagggtatta aacgcatcgg gttgatgacc
     4921 tgtcagtaaa aaatgttaca tgtatatttg tcactcttta cggccacgtt catttacata
     4981 aacacggctt atcgtatcgt ttttacacca aacggcccgc catgcagttg cgtgcatagg
     5041 catgcagcgg gttacaattt accgggcgcg tttccggcgg ttttccgggt ggtttgttgc
     5101 ctgttttatc ccgtagccgc cggaaacgcc ctcagaccgt ctgagcggtg cgggcaatgc
     5161 gacgttatgg taaatacccc ttaccggcgg cgctgtggct gccaggtgaa ggtggtttcc
     5221 gtagcctcgt atttctcccg acgcatttcc tgcgcccgcc tggcttcacg ttcgtcctga
     5281 atggcacgaa gcaccctttc ccgcacctgt tgttttaccg cctttggcag cgttttgttc
     5341 cctttcaggc ccatggccag tgcctgagcg acaaaggcgt ccgccaggcg cttttcttca
     5401 gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc gtctcaggtt tcccgaacag
     5461 tttaaccgca ggtcctctcc tcatgccagt tagctttcag atgtgtctat accatctgaa
     5521 atgtaagtgt gatgaatgtc ttaaataata aaatttgttg ccatgagaaa ttttcgggaa
     5581 aagacgcgat ctgcttacag gtggctccgg gcgctttttt gtgcctgaag atgagaacta
     5641 atctcatata ttgacacctg aaaacgggag gagtaaggta ataattatac tgtgtatatg
     5701 tacagtgtgc gccgggagac cggttagaga tcaaggggtg aaagtccccg accattgaag
     5761 gaccagcaat ccacaaggtc cccgagtcat gcgttgcata ccgcgaggta tggggcgaag
     5821 cgttgacagg ggtgttgaca ggccagccat tgagccacga aatgtatatt aaattaccgg
     5881 gtgccgacgt tgtactgtta acggaaggca acatcatagg gtgcgacact gcgagtgcca
     5941 catggacccg gcggggtctg agaccctggc atgtcaatac gatctctacg cgggaaccgg
     6001 gagatctccc ctctgaccat ctgccagtgt cggagatggc ccgcaccggg aagacgagga
     6061 gtcatagccg gtgatgtacg gagaggagaa gtcggactcg ctcatagtag cggcgaagca
     6121 ggcgaacaac ccgaaaggag cggagtcagt ggagcgaagg agcggggcca aggggaacgc
     6181 ggaacagcca cacatgcgcc ggacacagag ccgggaaagc atgtcacaga ggctgtcacg
     6241 cgtgcgggaa gctgcgaagc agcggaagaa agaacggttt acagcattgt tccacctgct
     6301 gacagtcgaa gcactggaag ccgcattcct ctccctgagc aggaaagcgg ccgccggagt
     6361 ggatggcatc aggtggatgg actacgccgg aaacatgaag aacaacataa cagatctgca
     6421 ccggaggcta catcagggca gctacagggc gcagcccggc aggcgtcact acatcccaaa
     6481 agcggatgga aaacaacgcc cgctcggcat cgcctcgctg gaggacaaga tcgtccagta
     6541 tgcgctggtg aaaatcctga acgcagtcta tgaaaacgac tttatggggt tctcatacgg
     6601 gttcagaccc gggcgaagcc agcacgatgc actggacgca ctggccacag ggctggtacg
     6661 cactaacgta aactgggtac tggatgccga catcagtcag ttcttcgaca gggtgagcca
     6721 cgaatggctg atcaggttca cagagcatcg gatcggcgac cggagggtaa tcaggctcat
     6781 acgtaagtgg ctcacagccg ggacgtcgga ggagggtcaa tggcgagcaa cggaggaagg
     6841 caccccacag ggtgcggtca tctcaccgct gctggcaaac atatacctcc actacgtctt
     6901 cgatctgtgg gcgcatcagt ggcgacgtcg ctatgccaca ggcaatgtgg taatggtcag
     6961 atacgccgat gacatcgtca tcgggttcga caaacgatac gatgcccggc gcttccgtat
     7021 agccatgcag cgcagactga gggagttcgg actcacggtt cacccggaga aaacccgtct
     7081 gatggagttc ggccgcttcg ctgccgaaaa ccgtgccatc aggggaaaag gcaaaccaga
     7141 aacgttcaac ttcctcgggt tcacgcacat cagcgggaaa gatcgcaacg gcaggttcat
     7201 gctgatacga aagacccgcc gggatcggat gacggcaact ctgaaagcca tcaaagacgg
     7261 tctgcgaagg cgctggcatt actcaatccc cgaacaggga aaatggctca ggagagtggt
     7321 tcagggatac ctgaactatc actcggtacc gggcaacttc cccaccatgc agaagttcag
     7381 gacacacgta acaaacctct ggcgccgggc gctcaggcgc aggagccaga aggatgatac
     7441 gacctggacg aaagcaaaca aactggcagc cgcatggcta ccaagggttc gggttcttca
     7501 tccatggcct gtggagcggt tcaccgccag acacccgagg caggagcccg gtgcgtaaat
     7561 cgcgcacgcc gggatctgtg cggggggtat ccggtaacgg gtatccctac cgcgacatgt
     7621 tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg gtatgagtgg tggagacggt
     7681 cgaggtccgg gtaattcagg tctgggacat aatggtggtc aggccagtgg gaatgtgaac
     7741 ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta cggatccaaa cagcgggccg
     7801 ggctggggta cgacgcatac gccgaatgga gatattcata actataatcc gggggagttt
     7861 ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca gcggcaatca tagcggtagc
     7921 tctggtggcg gacagtcttc ggccactgcg atggccttcg gtctgcctgc cctggctact
     7981 ccgggggctg aaggactggc tttatcagtt tccggcgatg cgttgtcggc cgccgttgct
     8041 gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc tgtgggggat tgcgatctac
     8101 ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa tgatgtcaaa aattatgacg
     8161 tcattaccgg ccgatgcggt gacggagact ccggcaagta ctttaccact ggaccaggcg
     8221 acggttcgtg tcagacaacg ggttgtggat gtggtgaagg atgagcggca gcatattgcg
     8281 gttgtcgcag gtcggccaat gagtgtccct gtggtggatg cgaaaccgac aaaacgtccg
     8341 ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc aggtgagcgt accgaaaggt
     8401 gttccgacag cgaaagcccc gccaaaaggc attgttgctg aaaaaggtga ttcacgtccg
     8461 gctggtttta cagccggtgg taactcccgt gaggccgtta ttcgtttccc gaaagagacc
     8521 ggacagaagc cggtttatgt gtcggtgaca gatgttctta ccccggcaca ggtaaaacag
     8581 cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg ccgctcatcc ggaagagggg
     8641 ctgaaaagag actatgataa agcgaaagct gagctggatg ccgaagataa aaatattgcg
     8701 accttaaaca gccgcattgc atcgacagag aaggcgctcc ccggtgcaag ggctgctgtt
     8761 caggaagccg ataaaaaggt aaaagaggca gaggcaaata aagatgattt tgtgacttat
     8821 aacccgcctc atgaatatgg ctccgggtgg caggatcagg ttcgctatct tgataaggat
     8881 attcagaatc agaatgagaa attaaaagcg gctcaggcat ctttaaacgc aatgaatgaa
     8941 tccttatcca gagataaggc tgcacttacc ggggcgatgg agagccggaa acaaaaggag
     9001 aaaaaagcga aggatgcaga aaataagtta aatgaggaaa agaacaagcc tcgcaagggg
     9061 actaaagact acggccatga ttatcatcca gtacctaaaa cagaagacat aaagggactt
     9121 ggagagttaa agcgaggtga tcctaaaaca cctaaacaag gtggtggtgg taagcgggcc
     9181 cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg attcccagca cggtgagctt
     9241 gaagggtatc gtgccagtga tggcgaacac ctaggcgcat ttgatcccaa aacgggtaag
     9301 caggttaaag ggccagatcc aaaacggaac attaaaaaat atctttgaga ggtaaatatg
     9361 gggcttaaat tacgtttgac atggtttgat aagaaaactg aagagtttaa aggggaagag
     9421 tattctaaag actttggtga cgatggttct gtcattgaaa gcttgggaat gcctttaaag
     9481 gataatatta ataatggtga gtttgatttg aagaaagaat gggttccttt actacagcca
     9541 ttttttaaaa atcaaataaa acctgatgtg gataattgtt atatagcttt tgattatcgg
     9601 gacggtaact ggtaacaaaa aattatcaag gatgatttga aatactgact tccggtgttc
     9661 agggatgaat gccggagtct ttaaatacag ggaaagtata tgaaaaaggt aaagactatt
     9721 tttcttttta tattgattgt tgctggcttt cttctggccg catgtcaggc aaattatatc
     9781 cgggatgttc agggcggaac ggtggcacca tcgtcctcct ctgaactgac ggggatcgcg
     9841 gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctttt
     9901 gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg
     9961 gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca
    10021 gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct
    10081 ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct ttcccgggtt
    10141 ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg
    10201 cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta
    10261 tgagaaagcg ccacacttcc cgcaagggag aacggcggac aggtatccgg taaacggcag
    10321 ggtcggaaca ggagagcgca agagggagcg acctaccgga aacggtgggg atctttaagt
    10381 cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg
    10441 caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg
    10501 ccaccggcgc ggccctgccg ttttgcctca catgttagtc ccctgcttat ccacggaatc
    10561 tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg
    10621 accgagtgag cgaggaggcg caatttcccc ggcacctgca agacggtcac cggacgcgct
    10681 gatgtgggat attgtcccct gattaagtct ttctattccc tgacgatcaa ttatcaggat
    10741 aggtgaacca tgtcggtttt tcgctgaaca ttttacgtta acctatctct gttgcttttt
    10801 tctccttgcg gagaaatgca agtcatcccc gctggggcgt ggctgacttc aggggaaaag
    10861 accttgctta cgctggctgg acgccaccga cgcacggtct tttcccctct ttctggctct
    10921 gtccggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga
    10981 ccacccacaa aaatccacgg tgctggacgc acctgacgaa gcagcaaagc tgctcctgat
    11041 tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa
    11101 gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg
    11161 gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgcggtga aagctgaacg
    11221 caatcaccac ggacgtgacc gccgccgcca tgccttgggg catgtccgcg gcttgtgcag
    11281 cgggggctgc gcatcacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa
    11341 tacggattcg cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg
    11401 ggtcagctgg ctgtaccggg agtattacag tctggccggt ttttttctgg ttatcggcct
    11461 gtttttcggt ctggctgaac tctgggccgg ccgaatgcgt tttgtggggc tgagccataa
    11521 ggcgcggcgg gagtatgccg atcgctataa gcgcgataat aaccggactg tgccattttc
    11581 ggtgctggcc tcgctggatt cacccgggcg gatggtcagg gcacggcgac atgtctggaa
    11641 ctacgcgtac tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc
    11701 cgtgtttatc gagagctggg gagaaaccgt cctgcgcccg gacctctatg cgaacgcaga
    11761 ttattacctg ctgcagccgg ctaacctgtt cgggatagcc tggtgtgccg gagggcttgt
    11821 cgccggcgtc cttctggcgc ggctgctggc gttcctgctg cttgatggtc attttgcggc
    11881 cgctgacgag gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac
    11941 cggagaaatg acagacgtcc gccacctgca
//
