LOCUS       NZ_CP136835            10060 bp    DNA     circular CON 26-OCT-2023
DEFINITION  Citrobacter koseri strain K2721 plasmid pK2721-3, complete sequence.
ACCESSION   NZ_CP136835
VERSION     NZ_CP136835.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37851753
            Assembly: GCF_032947135.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K2721 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/26/2023 13:14:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,750
            Genes (coding)                    :: 4,659
            CDSs (with protein)               :: 4,659
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 25 of 91
            Pseudo Genes (incomplete)         :: 65 of 91
            Pseudo Genes (internal stop)      :: 17 of 91
            Pseudo Genes (multiple problems)  :: 13 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K2721"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK2721-3"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collected_by="Jiayao Yao"
     gene            complement(118..267)
                     /locus_tag="R1243_RS23795"
                     /old_locus_tag="R1243_23795"
     CDS             complement(118..267)
                     /locus_tag="R1243_RS23795"
                     /old_locus_tag="R1243_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(352..609)
                     /locus_tag="R1243_RS23800"
                     /old_locus_tag="R1243_23800"
     CDS             complement(352..609)
                     /locus_tag="R1243_RS23800"
                     /old_locus_tag="R1243_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(619..2304)
                     /locus_tag="R1243_RS23805"
                     /old_locus_tag="R1243_23805"
     CDS             complement(619..2304)
                     /locus_tag="R1243_RS23805"
                     /old_locus_tag="R1243_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2631..2876
                     /locus_tag="R1243_RS23810"
                     /old_locus_tag="R1243_23810"
     CDS             2631..2876
                     /locus_tag="R1243_RS23810"
                     /old_locus_tag="R1243_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3309..3521
                     /locus_tag="R1243_RS23815"
                     /old_locus_tag="R1243_23815"
     CDS             3309..3521
                     /locus_tag="R1243_RS23815"
                     /old_locus_tag="R1243_23815"
                     /inference="COORDINATES: protein motif:HMM:NF018252.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="WP_250203192.1"
                     /translation="MFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVT
                     HAKIPPAPDENTWQIFKNAPLDFRP"
                     /name="cell envelope integrity TolA C-terminal
                     domain-containing protein"
     gene            3518..3883
                     /locus_tag="R1243_RS23820"
                     /old_locus_tag="R1243_23820"
     CDS             3518..3883
                     /locus_tag="R1243_RS23820"
                     /old_locus_tag="R1243_23820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            4058..4336
                     /locus_tag="R1243_RS23825"
                     /old_locus_tag="R1243_23825"
     CDS             4058..4336
                     /locus_tag="R1243_RS23825"
                     /old_locus_tag="R1243_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4324..4638
                     /locus_tag="R1243_RS23830"
                     /old_locus_tag="R1243_23830"
     CDS             4324..4638
                     /locus_tag="R1243_RS23830"
                     /old_locus_tag="R1243_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4802..5230
                     /locus_tag="R1243_RS23835"
                     /old_locus_tag="R1243_23835"
     CDS             4802..5230
                     /locus_tag="R1243_RS23835"
                     /old_locus_tag="R1243_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5256..5435
                     /locus_tag="R1243_RS23840"
                     /old_locus_tag="R1243_23840"
     CDS             5256..5435
                     /locus_tag="R1243_RS23840"
                     /old_locus_tag="R1243_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5462..5992)
                     /locus_tag="R1243_RS23845"
                     /old_locus_tag="R1243_23845"
     CDS             complement(5462..5992)
                     /locus_tag="R1243_RS23845"
                     /old_locus_tag="R1243_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5999..6730)
                     /gene="mobC"
                     /locus_tag="R1243_RS23850"
                     /old_locus_tag="R1243_23850"
     CDS             complement(5999..6730)
                     /gene="mobC"
                     /locus_tag="R1243_RS23850"
                     /old_locus_tag="R1243_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6730..8694)
                     /locus_tag="R1243_RS23855"
                     /old_locus_tag="R1243_23855"
     CDS             complement(6730..8694)
                     /locus_tag="R1243_RS23855"
                     /old_locus_tag="R1243_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7304
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9497..10060
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.236"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(6003..6730)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta
       61 cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      121 ctgaaccgcg atccccgtca gttcagagga ggacgatggt gccaccgttc cgccctgaac
      181 atcccggata taatttgcct gacatgcggc cagaagaaag ccagcaacaa tcaatataaa
      241 aagaaaaata gtctttacct ttttcatata ctttccctgt atttaaagac tccggcattc
      301 atccctgaac accggaagtc agtatttcaa atcatccttg ataatttttt gttaccagtt
      361 accgtcccga taatcaaaag ctatataaca attatccaca tcaggtttta tttgattttt
      421 aaaaaatggc tgtagtaaag gaacccattc tttcttcaaa tcaaactcac cattattaat
      481 attatccttt aaaggcattc ccaagctttc aatgacagaa ccatcgtcac caaagtcttt
      541 agaatactct tcccctttaa actcttcagt tttcttatca aaccatgtca aacgtaattt
      601 aagccccata tttacctctc aaagatattt tttaatgttc cgttttggat ctggcccttt
      661 aacctgctta cccgttttgg gatcaaatgc gcctaggtgt tcgccatcac tggcacgata
      721 cccttcaagc tcaccgtgct gggaatccca ttcataaatt ttacgttttt tatcgccata
      781 ccatcgggcc cgcttaccac caccaccttg tttaggtgtt ttaggatcac ctcgctttaa
      841 ctctccaagt ccctttatgt cttctgtttt aggtactgga tgataatcat ggccgtagtc
      901 tttagtcccc ttgcgaggct tgttcttttc ctcatttaac ttattttctg catccttcgc
      961 ttttttctcc ttttgtttcc ggctctccat cgccccggta agtgcagcct tatctctgga
     1021 taaggattca ttcattgcgt ttaaagatgc ctgagccgct tttaatttct cattctgatt
     1081 ctgaatatcc ttatcaagat agcgaacctg atcctgccac ccggagccat attcatgagg
     1141 cgggttataa gtcacaaaat catctttatt tgcctctgcc tcttttacct ttttatcggc
     1201 ttcctgaaca gcagcccttg caccggggag cgccttctct gtcgatgcaa tgcggctgtt
     1261 taaggtcgca atatttttat cttcggcatc cagctcagct ttcgctttat catagtctct
     1321 tttcagcccc tcttccggat gagcggcgtc ccatgcctgc tggcgacgct tttcttcctc
     1381 ctgacgctgt tttacctgtg ccggggtaag aacatctgtc accgacacat aaaccggctt
     1441 ctgtccggtc tctttcggga aacgaataac ggcctcacgg gagttaccac cggctgtaaa
     1501 accagccgga cgtgaatcac ctttttcagc aacaatgcct tttggcgggg ctttcgctgt
     1561 cggaacacct ttcggtacgc tcacctgcag agacgggaga cccggaatcg acacactgaa
     1621 tacccccgga cgttttgtcg gtttcgcatc caccacaggg acactcattg gccgacctgc
     1681 gacaaccgca atatgctgcc gctcatcctt caccacatcc acaacccgtt gtctgacacg
     1741 aaccgtcgcc tggtccagtg gtaaagtact tgccggagtc tccgtcaccg catcggccgg
     1801 taatgacgtc ataatttttg acatcatttt cggatcatct tttgcaatct cagaaggcag
     1861 cacaccgtag atcgcaatcc cccacagacc aaacttaaac ggccccttca gggcagccag
     1921 cacatcagca acggcggccg acaacgcatc gccggaaact gataaagcca gtccttcagc
     1981 ccccggagta gccagggcag gcagaccgaa ggccatcgca gtggccgaag actgtccgcc
     2041 accagagcta ccgctatgat tgccgctgtt accaccatta ccaccgggtt tactcccgcc
     2101 gttaccaaac tcccccggat tatagttatg aatatctcca ttcggcgtat gcgtcgtacc
     2161 ccagcccggc ccgctgtttg gatccgtacc accacctgat gaagggccac ctttaccaga
     2221 cgtaccgttc acattcccac tggcctgacc accattatgt cccagacctg aattacccgg
     2281 acctcgaccg tctccaccac tcataccgtt ttcctctttg acatttaaaa caataaatta
     2341 aagccaacat actgtacata tacacagtat aattattacc ttactcctcc cgttttcagg
     2401 tgtcaatata tgagattagt tctcatcttc aggcacaaaa aagcgcccgg agccacctgt
     2461 aagcagatcg cgtcttttcc cgaaaatttc tcatggcaac aaattttatt atttaagaca
     2521 ttcatcacac ttacatttca gatggtatag acacatctga aagctaactg gcatgaggag
     2581 aggacctgcg gttaaactgt tcgggaaacc tgagacgccc ggaggccact atgacgcacc
     2641 tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct caggcactgg
     2701 ccatgggcct gaaagggaac aaaacgctgc caaaggcggt aaaacaacag gtgcgggaaa
     2761 gggtgcttcg tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa atgcgtcggg
     2821 agaaatacga ggctacggaa accaccttca cctggcagcc acagcgccgc cggtaagggg
     2881 tatttaccat aacgtcgcat tgcccgcacc gctcagacgg tctgagggcg tttccggcgg
     2941 ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg cccggtaaat
     3001 tgtaacccgc tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt gtaaaaacga
     3061 tacgataagc cgtgtttatg taaatgaacg tggccgtaaa gagtgacaaa tatacatgta
     3121 acatttttta ctgacaggtc atcaacccga tgcgtttaat accctttatc ctgctggcat
     3181 tgtgcagccc cttactcctg acggcctgta agcattccac cacccagccg ccgaaaattt
     3241 atactctgga taagtcatct aaaatcagta gctacgctgc ggccatcaag accgctgttg
     3301 aaatgcagat gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg cggatatcca
     3361 tacaacgtga tggcactctg aacagcgcca ttgctgaagg aggagatccg aaactctgta
     3421 aagcagcaat atcagccgtt acccacgcca aaattccgcc ggcaccggat gaaaatacct
     3481 ggcaaatatt caaaaacgcc cctctggatt tcagaccatg aaagctttta ctctgacagt
     3541 gctggcggcc atgttcctca ctccggtaca tgcgatacca ctaaaacccg taaccacact
     3601 gcagccaacc tacccggtac aggccgcagc aaaacgcatc gaaggtcagg tgaccgccac
     3661 gtttgatatt ctggacgatg gacgagtgga caacgtgtcg cttaccggct gtcacctttt
     3721 ttttcgggaa acgctgacgg caatgaggca ctggctatat cccgcagggc acccggcaat
     3781 gcgacaggtt ataaattttg aattcagtct gaaaaatgac ggcttacagg atccgcgacc
     3841 agtcaccacc caaaagctca atgatgatgg ttgccgccag taacaggtcc tttaacccgc
     3901 aggctgcaat agtcctggat gatgagtgag cctgtagcag ccctgattag tggaagcgaa
     3961 gctgaaaaat gggcagcaaa agccgtattt ctggcaaata gcgccgccac ggattaaagt
     4021 agtcatgtgt aatacatatg actacaaagg ggcttttatg tcatcagcaa catccatcag
     4081 gctcgatgaa gaacttaagg accggctgaa aacgctggcc gatgaccggc accgctcagc
     4141 gcacgcgcta atgctggagg caatcaccga atacatcgac cgggaagaga agcgcagcca
     4201 gtacctgcgg gacggacagg ccgcatggca gcactatcag gaaacgggtc tgcaccttac
     4261 tgcggaagaa gcggaagcct ggataagtac ctggggaact gagaacgagc aggatgctcc
     4321 gccatgccac aggtaacgat ttccgcgctg gcacagcgcg atttacaacg ccttcaggac
     4381 tttctcaaaa ccaaaaatcg gctggcagcc agaaaaggag gtgaggtgat cgtccgggct
     4441 atccagcaac tgaaaacatt gccagacatt ggccgcccgg tgccgtttct gccgctggaa
     4501 tataaggaac tggtgatcgg gtttggcgac agtggctatg tgatgctcta ccgccacgac
     4561 cgggaaatgg accagattgt gatcgtcacc gtcaggcatc agaaagaatc cgggtatccg
     4621 ggggcagaca gcctctaata accgatgacg ggggcgcaat tcattgcgtg gccgtcgccg
     4681 gtttgacaag cgcagcgcgt cagaaaacca catcctgaac ggaaaaacgg gtaaaattca
     4741 gcgatacatt taccgtgaga aattacacac ctttcacacc cgataaaatc aggggtatac
     4801 agtggcgaaa acattgctca acctttcaca ggcagcacaa gccgcgggca ttaccagaag
     4861 aacgctatac aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg ggaaaaacaa
     4921 ccctgtcgtg gatgtgtcag aactgatccg tgtatacgga aatgtgaata tccctgagaa
     4981 acaaattccc ggcatttcac accgtgaaaa tacacaaaaa aatttcccac aggaacagct
     5041 tctggcaatg caaaaggaac tggccgatct ccggcaggct gtcaccctga tgctggaaga
     5101 taaaacatcc cgggaagaag aacgccgcca gcacgatgat gagcgccgga aactccaggc
     5161 cgaagtggac agattgacaa cagaactgac acagaagaaa aaacgcttct ggtccggctg
     5221 gtttagctga gaacttaccg aagggaatgg agaacatgac gaagcaggaa aaaactgccc
     5281 tcaatatggc taaattcatt aaggatcagt cgctgctgtt gctggaaaag ctgaacgagc
     5341 tggacatgga taccccggcc gacatgtgcg aacggcttca cgaggatgcg gaaacactct
     5401 ggcaggtgat gcgggataag ctcggggaag aataacagca ccggccatgc cggtgctggc
     5461 gttatttttt gccgaacggc caccaccggc gggcgggttt gtcctccggc agttcaggca
     5521 ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag ctccgctttc agcttctcgt
     5581 tcaccgcgct ttccccgtgc cgctcgctga ccacctcaat catcgcgtta atctgctccg
     5641 gtgagcactt cccggcatca accacctgca caccgttctc gatggcgaac cgctccacct
     5701 ttcgatagag gtaatcacac agcttcattc ctgtgggttt gtcccgttcc cattgcggat
     5761 tttcaccggt gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc
     5821 cgatggattt catcatcgcc agatgctggg cccggtcgac aaaacagaaa acatacgccc
     5881 cggtctgcca ggcgagcacc accatgtctt ccagatgctc gctggttggc ataatgcagc
     5941 gctcgccgtc tttgtcttcc acgtcctcaa taaataccat gtacagcgcc ataatccctc
     6001 actccgttgt cataaattta aagcacgcga acagcctctc ccggtccagc tccaccgttt
     6061 tgcgggcctc gccggtctgt ggattcacga tttcatccac cgccagaatc gcccgctgta
     6121 cccggacaag cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc
     6181 ggcggttgaa cagatacccg gacaccactt cctgataccg cctgcggctc ttgacggtgc
     6241 gctctacctc aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc ggcactttca
     6301 tccctttcag ggccatacgg tgcaggtacc gccagccggt ggccccccgg ccctccatgg
     6361 agaggcgcag ggactgcacg gccagccggt gcggcagctg cgccgccgac acccttgacg
     6421 gctgaaacgg aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt
     6481 gcggcgtcag cccccacacg ctgacctgac ggccgtcaac cgggtatttc gcccggacca
     6541 gcagcccttt cgccaccaga ctgtccagga agcggtaaac cggctgtacc gccgcaatcc
     6601 ccagccgtcg cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg
     6661 tcagccgtgt tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct
     6721 ccagtgtcat cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc
     6781 cggaggcagg tactccgcca gcgggtccgt tttcgccagc accgggaagg ttttcagcga
     6841 aatttcccgg cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca cgcccagccc
     6901 ggtcatcacc gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg
     6961 cgcctccttc tggatgaagc tgtcgccgct gatgtgctcc acgttccccg cctcccggct
     7021 ggcccggcgg cgctccacgt ccacccggat ctccccggtc tggcccgccg cccactgcgc
     7081 ggactcctgg ctggccgcgc gatagaacca gcggatcggc gtgttatcca gtaccgtggt
     7141 tttcacaaaa tcagcgggca gatcctgccc gcactgaccg aagtcgccca gcgactggtg
     7201 cgcgagcagc aggttgcagt cccggctgcg cagcgtcccc agcgcgttca gcacgtactt
     7261 cgacagcaga tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc
     7321 atcccgcgca atgatgattt gcgcgcagcg ggcaaacagc atcttctgca cccggatcac
     7381 cgcctcatcg tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc
     7441 cacatcgata ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt
     7501 gatcagcccc ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg ccgccagcac
     7561 ctgctgaatg tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc
     7621 gctgatgcgg tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag
     7681 cagattcttc agggcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc
     7741 cggccgcaga tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca
     7801 cgcgtcccct ttggggtcga agtacaccac agcgtccccg aaatcgcgta cgcagcgaat
     7861 gagcgcgttg gtggccatcg cgctcttgcc gctcccgggc aggccgagga tctgaatatt
     7921 ggtcttgcgc caggtatcac gagggaccag caccggctgg ccctgctcat ccttacccag
     7981 aaacaccgcc tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg
     8041 taccggctcc ccgaaatgca ggtggcggac gtctgtcatt tctccggtcc gctggctgct
     8101 gcgccggctc tcctgattca gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc
     8161 atcaagcagc aggaacgcca gcagccgcgc cagaaggacg ccggcgacaa gccctccggc
     8221 acaccaggct atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcata
     8281 gaggtccggg cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc
     8341 ccccagaagg gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgtcg
     8401 ccgtgccctg accatccgcc cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt
     8461 ccggttatta tcgcgcttat agcgatcggc atactcccgc cgcgccttat ggctcagccc
     8521 cacaaaacgc attcggccgg cccagagttc agccagaccg aaaaacaggc cgataaccag
     8581 aaaaaaaccg gccagactgt aatactcccg gtacagccag ctgacccacg ggtaaatctg
     8641 ccgggccagc tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc
     8701 tctctccttc agacgacggg tttaccggcg tgatgcgcag cccccgctgc acaagccgcg
     8761 gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac
     8821 cgcggaggcc agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga
     8881 gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc
     8941 ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt
     9001 tgctgcttcg tcaggtgcgt ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct
     9061 gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccggacagag ccagaaagag
     9121 gggaaaagac cgtgcgtcgg tggcgtccag ccagcgtaag caaggtcttt tcccctgaag
     9181 tcagccacgc cccagcgggg atgacttgca tttctccgca aggagaaaaa agcaacagag
     9241 ataggttaac gtaaaatgtt cagcgaaaaa ccgacatggt tcacctatcc tgataattga
     9301 tcgtcaggga atagaaagac ttaatcaggg gacaatatcc cacatcagcg cgtccggtga
     9361 ccgtcttgca ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggg
     9421 cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa
     9481 gcaggggact aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt tttccatagg
     9541 ctccgccctc ctgccagagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc
     9601 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
     9661 accgtttccg gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
     9721 atacctgtcc gccgttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacacactg
     9781 gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt
     9841 tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cgggaaagca
     9901 cgcaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc
     9961 taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt
    10021 tggttgctgt gctctgcgaa agccagttac cacggttaag
//
