LOCUS       NZ_CP136833             2953 bp    DNA     circular CON 26-OCT-2023
DEFINITION  Citrobacter koseri strain K2721 plasmid pK2721-4, complete sequence.
ACCESSION   NZ_CP136833
VERSION     NZ_CP136833.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37851753
            Assembly: GCF_032947135.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K2721 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/26/2023 13:14:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,750
            Genes (coding)                    :: 4,659
            CDSs (with protein)               :: 4,659
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 25 of 91
            Pseudo Genes (incomplete)         :: 65 of 91
            Pseudo Genes (internal stop)      :: 17 of 91
            Pseudo Genes (multiple problems)  :: 13 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136833.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K2721"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK2721-4"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collected_by="Jiayao Yao"
     gene            418..969
                     /locus_tag="R1243_RS23520"
                     /old_locus_tag="R1243_23520"
     CDS             418..969
                     /locus_tag="R1243_RS23520"
                     /old_locus_tag="R1243_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     gene            966..1316
                     /locus_tag="R1243_RS23525"
                     /old_locus_tag="R1243_23525"
     CDS             966..1316
                     /locus_tag="R1243_RS23525"
                     /old_locus_tag="R1243_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(1343..2317)
                     /gene="mobV"
                     /locus_tag="R1243_RS23530"
                     /old_locus_tag="R1243_23530"
     CDS             complement(1343..2317)
                     /gene="mobV"
                     /locus_tag="R1243_RS23530"
                     /old_locus_tag="R1243_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
ORIGIN
        1 tgcacccctg ccacgtggcg aacgcggggg gtggcggggg gcagcgccca ccgccccaag
       61 gctgcgcagc ggccgccgga ggccccccaa acaacgtcac ccggccggtg gccgggtggg
      121 ccttttccag aggccccgca ggggccgtca gcggggtgcc aaggccgcta cgcggccgcc
      181 ggcggggcgg cagcccctta gaggagaaag ggggaaatgc taagttattg atttataagg
      241 atttgtagaa atgcattgag acacataggg tgtcctaacg ttgagacatg catggatgtc
      301 tcaacaggta gcaaggttga gacgcaaact gtctcgacgt gagacataat ttgtcgtatc
      361 attgcgactc gaaatggcgc aatgagcgga caaatttccc gactttggag gaaccccatg
      421 cagcagatca cggtggcgac cgacgcccag cgtcgtatcc gccagcgaga gctggccatg
      481 ctggacgaga aagaggctca ggagcgcgaa gaggcgcgga agaacaaggg gttcacgcag
      541 gtgtatccct tgggttggaa gcgcatcatt gagcttgcta aaggcaatgc tggggccttc
      601 ggcctgtatt cattctttgc tgaaaatatc gacccaacct gcggcgcggt ggtctgcgat
      661 cagcagtttt tggcagacaa gatgaacgtc aacaggcgga caatcagccg ttggttgtct
      721 tacctggaag aaaatcgcgc gcttgtccgc atccctgttg ctggcaaggt ttgcgcctat
      781 gcgctggatc ctcacgaggt ctggaagggc tacgacaacg ccaaggatta cgcagctttc
      841 gtgaccaaaa ccttggtcaa caaggatggc gatatccgcc gccggatcat gtcgatgttc
      901 agcggcgaga agcagaacga cgaccgcgac ccgaacacca ttgacatgtt tgaaagcact
      961 ccccaatgag caacgaaacg aaggagaaaa tctttatgaa aaaaacgttc gctcttgctg
     1021 ccatgatggc ggcaatcgct ccgaatcttg cttcagcagc ggatgagatg atccagacca
     1081 agtacgcttg cgaaggcgga aagacgttgg atgtcgtgta cgtcggagac tatgccgtga
     1141 tgctgcaaat ggatgagctt gtcccactga aacgcgccaa gagcgcatca ggagtgcgct
     1201 acatctccgc tagcaaaaac tacacctacg aactgtgggg taagggcaac gacatgaacc
     1261 tttcgaccca cgatggcggg aaggagcaaa ccgtcctcag tagctgcaag ccataaaccc
     1321 cttccgtcgg ccacctgtag ggtcaacgag acagcccgcc gtcgtcgcga tcccggccgc
     1381 gactgcgaag ccgttgggcc tgccgctggc gtgtcgcctc gcgctgttcg gcgagcagct
     1441 tctcgcccac ggccttgatg atctgggccg ccttggcctg catatcgcga ttaagcggcc
     1501 ccagagcgtc cagaacgggc ttcaggcggt cacgcaggct ccgggctgtc tcctgtgcct
     1561 gccgggcctt ctcgcgctcc tgacgcgctc ctgcggccgc tgcgacggtc gggccatacc
     1621 cttccctgac ggcagcggtc acgcgggcgg ccacggcctc cggcgtctcc acgtccttcg
     1681 agaaaatccc cttgcgcagc acccgcggct cgatggcctg cggcgtgatg gtggcgtgcc
     1741 cgggctggcg ctcgatggcg ccgtagtagc tctggacgcg ctgatgggtg gcccggctgc
     1801 cctcgatgcc gcgttccagc ccgagatccc tcaccgcctc ggcaaagctg gtctggtcgg
     1861 cccgcatctt gtcgcggcca ccgatgaact ccttggccga cagccgcccg tcctgcgtca
     1921 gcggcacgac gaaggccgac aggtgcgggg tggcctcgtc gcggtggatg ctggccgtca
     1981 cgatgcgatc cgcgccgtat ttggccgcca gccagccctg cgcctggtcg aagaacgcgg
     2041 cctgctgctg gggcgtggcc ttggcccacc attccgggct ggccgtcatc acgtattcga
     2101 ccgccagcac cgcgtccttg cgccgcttct ccggcagcaa ctcgcgcagc cgccccatcg
     2161 ccgcatcggt tgaggtggcc gcccggtgct cgttgtccgg cgtgcgttcg gcgtcagcgt
     2221 tgggcgtttc gcgctcgcgg tagcagtgct gcaacgccgc cgccacgctg cccatcgtcg
     2281 ccagcttctt gcatcgcatg atcgcgtagg ccgccatgcc tgcccctccc cttggtgtcc
     2341 gaatccggcc agtcggggca gcgcaaggcg gtgcctccgg cgggccacta tcagatgata
     2401 gtgtactcac tagactttgc gaagcaaagt cgtgcccgcc tgcggcggtt gcagcgccct
     2461 tcgggcttgc tcctgcggcg gccttcggcc ttgcccaaag gggctttgcc cctctggact
     2521 ccccaccctc gccgccggga ggctcggggg gcaggggtgg cgggcttcgc ccttcgtccc
     2581 tgcccccact cgcacaggct ggggcgctgc ggtcagcgtc aagggttcgg cttcgcccgc
     2641 gtcctcgccc ggtccctgcg cttcgctccg ggctgcggcc tgcgggcgcg cccttgaccc
     2701 tgccctccgc tgaaggcgtc agccagtggc ccgctggcgt gtcggaaatc gcctcatggc
     2761 cgggtaggaa atttccgaca gcgccggagt ggaaggcggg tcaagggcgc agggggtggc
     2821 tggcgcggcc cgcagcccgc agggcgagga cacgggccac ccccggagag cgcagcggcc
     2881 cttgacgcgc ctggaacgac cccacgctcc ccggtagggg tgcaggagtg aagggccgca
     2941 ggcccgtagc ccc
//
