LOCUS       NZ_CP136831             2953 bp    DNA     circular CON 26-OCT-2023
DEFINITION  Citrobacter koseri strain K2521 plasmid pK2521-4, complete sequence.
ACCESSION   NZ_CP136831
VERSION     NZ_CP136831.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37851623
            Assembly: GCF_032946055.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K2521 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/26/2023 13:13:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,750
            Genes (coding)                    :: 4,658
            CDSs (with protein)               :: 4,658
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 25 of 92
            Pseudo Genes (incomplete)         :: 66 of 92
            Pseudo Genes (internal stop)      :: 17 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136831.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K2521"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK2521-4"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collected_by="Jiayao Yao"
     gene            join(2897..2953,1..495)
                     /locus_tag="R1917_RS24320"
                     /old_locus_tag="R1917_24320"
     CDS             join(2897..2953,1..495)
                     /locus_tag="R1917_RS24320"
                     /old_locus_tag="R1917_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033995415.1"
                     /translation="MQQITVATDAQRRIRQRELAMLDEKEAQEREEARKNKGFTQVYPL
                     GWKRIIELAKGNAGAFGLYSFFAENIDPTCGAVVCDQQFLADKMNVNRRTISRWLSYLE
                     ENRALVRIPVAGKVCAYALDPHEVWKGYDNAKDYAAFVTKTLVNKDGDIRRRIMSMFSG
                     EKQNDDRDPNTIDMFESTPQ"
                     /name="RepA plasmid replication protein"
     gene            492..842
                     /locus_tag="R1917_RS24325"
                     /old_locus_tag="R1917_24325"
     CDS             492..842
                     /locus_tag="R1917_RS24325"
                     /old_locus_tag="R1917_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="WP_077265610.1"
                     /translation="MSNETKEKIFMKKTFALAAMMAAIAPNLASAADEMIQTKYACEGG
                     KTLDVVYVGDYAVMLQMDELVPLKRAKSASGVRYISASKNYTYELWGKGNDMNLSTHDG
                     GKEQTVLSSCKP"
                     /name="MliC family protein"
     gene            complement(869..1843)
                     /gene="mobV"
                     /locus_tag="R1917_RS24330"
                     /old_locus_tag="R1917_24330"
     CDS             complement(869..1843)
                     /gene="mobV"
                     /locus_tag="R1917_RS24330"
                     /old_locus_tag="R1917_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_033995413.1"
                     /translation="MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHR
                     AATSTDAAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLA
                     AKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRDKMRADQTSFAEAVR
                     DLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKGIFSKDVETPEAV
                     AARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQA
                     KAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR"
                     /name="mobV"
ORIGIN
        1 gccatgctgg acgagaaaga ggctcaggag cgcgaagagg cgcggaagaa caaggggttc
       61 acgcaggtgt atcccttggg ttggaagcgc atcattgagc ttgctaaagg caatgctggg
      121 gccttcggcc tgtattcatt ctttgctgaa aatatcgacc caacctgcgg cgcggtggtc
      181 tgcgatcagc agtttttggc agacaagatg aacgtcaaca ggcggacaat cagccgttgg
      241 ttgtcttacc tggaagaaaa tcgcgcgctt gtccgcatcc ctgttgctgg caaggtttgc
      301 gcctatgcgc tggatcctca cgaggtctgg aagggctacg acaacgccaa ggattacgca
      361 gctttcgtga ccaaaacctt ggtcaacaag gatggcgata tccgccgccg gatcatgtcg
      421 atgttcagcg gcgagaagca gaacgacgac cgcgacccga acaccattga catgtttgaa
      481 agcactcccc aatgagcaac gaaacgaagg agaaaatctt tatgaaaaaa acgttcgctc
      541 ttgctgccat gatggcggca atcgctccga atcttgcttc agcagcggat gagatgatcc
      601 agaccaagta cgcttgcgaa ggcggaaaga cgttggatgt cgtgtacgtc ggagactatg
      661 ccgtgatgct gcaaatggat gagcttgtcc cactgaaacg cgccaagagc gcatcaggag
      721 tgcgctacat ctccgctagc aaaaactaca cctacgaact gtggggtaag ggcaacgaca
      781 tgaacctttc gacccacgat ggcgggaagg agcaaaccgt cctcagtagc tgcaagccat
      841 aaaccccttc cgtcggccac ctgtagggtc aacgagacag cccgccgtcg tcgcgatccc
      901 ggccgcgact gcgaagccgt tgggcctgcc gctggcgtgt cgcctcgcgc tgttcggcga
      961 gcagcttctc gcccacggcc ttgatgatct gggccgcctt ggcctgcata tcgcgattaa
     1021 gcggccccag agcgtccaga acgggcttca ggcggtcacg caggctccgg gctgtctcct
     1081 gtgcctgccg ggccttctcg cgctcctgac gcgctcctgc ggccgctgcg acggtcgggc
     1141 catacccttc cctgacggca gcggtcacgc gggcggccac ggcctccggc gtctccacgt
     1201 ccttcgagaa aatccccttg cgcagcaccc gcggctcgat ggcctgcggc gtgatggtgg
     1261 cgtgcccggg ctggcgctcg atggcgccgt agtagctctg gacgcgctga tgggtggccc
     1321 ggctgccctc gatgccgcgt tccagcccga gatccctcac cgcctcggca aagctggtct
     1381 ggtcggcccg catcttgtcg cggccaccga tgaactcctt ggccgacagc cgcccgtcct
     1441 gcgtcagcgg cacgacgaag gccgacaggt gcggggtggc ctcgtcgcgg tggatgctgg
     1501 ccgtcacgat gcgatccgcg ccgtatttgg ccgccagcca gccctgcgcc tggtcgaaga
     1561 acgcggcctg ctgctggggc gtggccttgg cccaccattc cgggctggcc gtcatcacgt
     1621 attcgaccgc cagcaccgcg tccttgcgcc gcttctccgg cagcaactcg cgcagccgcc
     1681 ccatcgccgc atcggttgag gtggccgccc ggtgctcgtt gtccggcgtg cgttcggcgt
     1741 cagcgttggg cgtttcgcgc tcgcggtagc agtgctgcaa cgccgccgcc acgctgccca
     1801 tcgtcgccag cttcttgcat cgcatgatcg cgtaggccgc catgcctgcc cctccccttg
     1861 gtgtccgaat ccggccagtc ggggcagcgc aaggcggtgc ctccggcggg ccactatcag
     1921 atgatagtgt actcactaga ctttgcgaag caaagtcgtg cccgcctgcg gcggttgcag
     1981 cgcccttcgg gcttgctcct gcggcggcct tcggccttgc ccaaaggggc tttgcccctc
     2041 tggactcccc accctcgccg ccgggaggct cggggggcag gggtggcggg cttcgccctt
     2101 cgtccctgcc cccactcgca caggctgggg cgctgcggtc agcgtcaagg gttcggcttc
     2161 gcccgcgtcc tcgcccggtc cctgcgcttc gctccgggct gcggcctgcg ggcgcgccct
     2221 tgaccctgcc ctccgctgaa ggcgtcagcc agtggcccgc tggcgtgtcg gaaatcgcct
     2281 catggccggg taggaaattt ccgacagcgc cggagtggaa ggcgggtcaa gggcgcaggg
     2341 ggtggctggc gcggcccgca gcccgcaggg cgaggacacg ggccaccccc ggagagcgca
     2401 gcggcccttg acgcgcctgg aacgacccca cgctccccgg taggggtgca ggagtgaagg
     2461 gccgcaggcc cgtagcccct gcacccctgc cacgtggcga acgcgggggg tggcgggggg
     2521 cagcgcccac cgccccaagg ctgcgcagcg gccgccggag gccccccaaa caacgtcacc
     2581 cggccggtgg ccgggtgggc cttttccaga ggccccgcag gggccgtcag cggggtgcca
     2641 aggccgctac gcggccgccg gcggggcggc agccccttag aggagaaagg gggaaatgct
     2701 aagttattga tttataagga tttgtagaaa tgcattgaga cacatagggt gtcctaacgt
     2761 tgagacatgc atggatgtct caacaggtag caaggttgag acgcaaactg tctcgacgtg
     2821 agacataatt tgtcgtatca ttgcgactcg aaatggcgca atgagcggac aaatttcccg
     2881 actttggagg aaccccatgc agcagatcac ggtggcgacc gacgcccagc gtcgtatccg
     2941 ccagcgagag ctg
//
