LOCUS       NZ_CP136827            10060 bp    DNA     circular CON 26-OCT-2023
DEFINITION  Citrobacter koseri strain K2521 plasmid pK2521-3, complete sequence.
ACCESSION   NZ_CP136827
VERSION     NZ_CP136827.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37851623
            Assembly: GCF_032946055.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K2521 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/26/2023 13:13:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,750
            Genes (coding)                    :: 4,658
            CDSs (with protein)               :: 4,658
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 25 of 92
            Pseudo Genes (incomplete)         :: 66 of 92
            Pseudo Genes (internal stop)      :: 17 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136827.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K2521"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK2521-3"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collected_by="Jiayao Yao"
     gene            join(8990..10060,1..894)
                     /locus_tag="R1917_RS00005"
                     /old_locus_tag="R1917_00005"
     CDS             join(8990..10060,1..894)
                     /locus_tag="R1917_RS00005"
                     /old_locus_tag="R1917_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            894..1625
                     /gene="mobC"
                     /locus_tag="R1917_RS00010"
                     /old_locus_tag="R1917_00010"
     CDS             894..1625
                     /gene="mobC"
                     /locus_tag="R1917_RS00010"
                     /old_locus_tag="R1917_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1632..2162
                     /locus_tag="R1917_RS00015"
                     /old_locus_tag="R1917_00015"
     CDS             1632..2162
                     /locus_tag="R1917_RS00015"
                     /old_locus_tag="R1917_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2189..2368)
                     /locus_tag="R1917_RS00020"
                     /old_locus_tag="R1917_00020"
     CDS             complement(2189..2368)
                     /locus_tag="R1917_RS00020"
                     /old_locus_tag="R1917_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2394..2822)
                     /locus_tag="R1917_RS00025"
                     /old_locus_tag="R1917_00025"
     CDS             complement(2394..2822)
                     /locus_tag="R1917_RS00025"
                     /old_locus_tag="R1917_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2986..3300)
                     /locus_tag="R1917_RS00030"
                     /old_locus_tag="R1917_00030"
     CDS             complement(2986..3300)
                     /locus_tag="R1917_RS00030"
                     /old_locus_tag="R1917_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3288..3566)
                     /locus_tag="R1917_RS00035"
                     /old_locus_tag="R1917_00035"
     CDS             complement(3288..3566)
                     /locus_tag="R1917_RS00035"
                     /old_locus_tag="R1917_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3741..4106)
                     /locus_tag="R1917_RS00040"
                     /old_locus_tag="R1917_00040"
     CDS             complement(3741..4106)
                     /locus_tag="R1917_RS00040"
                     /old_locus_tag="R1917_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4103..4315)
                     /locus_tag="R1917_RS00045"
                     /old_locus_tag="R1917_00045"
     CDS             complement(4103..4315)
                     /locus_tag="R1917_RS00045"
                     /old_locus_tag="R1917_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="WP_250203192.1"
                     /translation="MFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVT
                     HAKIPPAPDENTWQIFKNAPLDFRP"
                     /name="cell envelope integrity TolA C-terminal
                     domain-containing protein"
     gene            complement(4748..4993)
                     /locus_tag="R1917_RS00050"
                     /old_locus_tag="R1917_00050"
     CDS             complement(4748..4993)
                     /locus_tag="R1917_RS00050"
                     /old_locus_tag="R1917_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5320..7005
                     /locus_tag="R1917_RS00055"
                     /old_locus_tag="R1917_00055"
     CDS             5320..7005
                     /locus_tag="R1917_RS00055"
                     /old_locus_tag="R1917_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7015..7272
                     /locus_tag="R1917_RS00060"
                     /old_locus_tag="R1917_00060"
     CDS             7015..7272
                     /locus_tag="R1917_RS00060"
                     /old_locus_tag="R1917_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7357..7506
                     /locus_tag="R1917_RS00065"
                     /old_locus_tag="R1917_00065"
     CDS             7357..7506
                     /locus_tag="R1917_RS00065"
                     /old_locus_tag="R1917_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             320..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7610..8188)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          895..1622
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 agcgagcaga aagcggcgaa gctgattgcg gagcagttcc cgcagggggc aaacattcag
       61 caggtgctgg cggcggcgta cgcgttgccg gaggccctga aaaaggacgt gaaggggctg
      121 atcaccaagc tggagaacgt ggccgacctg agcgtcctgc agacggacag cggtatcgat
      181 gtggcgggga taatcaacgg cggcggctgc ctgtacgtca tcggctcgat ggacgatgag
      241 gcggtgatcc gggtgcagaa gatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg
      301 gatgagttcc ggaggtggcc gcacgcgagc atcatgctcg atgagattaa atatctgctg
      361 tcgaagtacg tgctgaacgc gctggggacg ctgcgcagcc gggactgcaa cctgctgctc
      421 gcgcaccagt cgctgggcga cttcggtcag tgcgggcagg atctgcccgc tgattttgtg
      481 aaaaccacgg tactggataa cacgccgatc cgctggttct atcgcgcggc cagccaggag
      541 tccgcgcagt gggcggcggg ccagaccggg gagatccggg tggacgtgga gcgccgccgg
      601 gccagccggg aggcggggaa cgtggagcac atcagcggcg acagcttcat ccagaaggag
      661 gcgcggccgc tgtttgacgt gaacaccctg caacacctgc ctgacgggtt cgcggtgatg
      721 accgggctgg gcgtggcgcg gctggcgttc agcagcccgc tgcgggttga gcgccgggaa
      781 atttcgctga aaaccttccc ggtgctggcg aaaacggacc cgctggcgga gtacctgcct
      841 ccggccgacg gcggcggacc aaaagcggat gatgattttg cggggcttta ctgatgacac
      901 tggagcagta caacgtcagc cacgcgaagc ggcaggcgcg gaacgcggag aaaacacggc
      961 tgaccctgcg ctggctgcgg gaagagctgt gttccacggc ggagctggtt gcgcgacggc
     1021 tggggattgc ggcggtacag ccggtttacc gcttcctgga cagtctggtg gcgaaagggc
     1081 tgctggtccg ggcgaaatac ccggttgacg gccgtcaggt cagcgtgtgg gggctgacgc
     1141 cgcacggggt ggcgttctcg ttcgacgagg acgagccgct gacggacgtt attccgtttc
     1201 agccgtcaag ggtgtcggcg gcgcagctgc cgcaccggct ggccgtgcag tccctgcgcc
     1261 tctccatgga gggccggggg gccaccggct ggcggtacct gcaccgtatg gccctgaaag
     1321 ggatgaaagt gccggacgcg ctggcggagc tggacggcag aacggtggca tttgaggtag
     1381 agcgcaccgt caagagccgc aggcggtatc aggaagtggt gtccgggtat ctgttcaacc
     1441 gccgggcgaa cggcatcgat gaaatctggt acatctgccc tgaccggccg acgcttgtcc
     1501 gggtacagcg ggcgattctg gcggtggatg aaatcgtgaa tccacagacc ggcgaggccc
     1561 gcaaaacggt ggagctggac cgggagaggc tgttcgcgtg ctttaaattt atgacaacgg
     1621 agtgagggat tatggcgctg tacatggtat ttattgagga cgtggaagac aaagacggcg
     1681 agcgctgcat tatgccaacc agcgagcatc tggaagacat ggtggtgctc gcctggcaga
     1741 ccggggcgta tgttttctgt tttgtcgacc gggcccagca tctggcgatg atgaaatcca
     1801 tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt ttcaccggtg
     1861 aaaatccgca atgggaacgg gacaaaccca caggaatgaa gctgtgtgat tacctctatc
     1921 gaaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt ggttgatgcc gggaagtgct
     1981 caccggagca gattaacgcg atgattgagg tggtcagcga gcggcacggg gaaagcgcgg
     2041 tgaacgagaa gctgaaagcg gagctgatgc gggaatataa cagccgctat tttaacgtgc
     2101 cggtgcctga actgccggag gacaaacccg cccgccggtg gtggccgttc ggcaaaaaat
     2161 aacgccagca ccggcatggc cggtgctgtt attcttcccc gagcttatcc cgcatcacct
     2221 gccagagtgt ttccgcatcc tcgtgaagcc gttcgcacat gtcggccggg gtatccatgt
     2281 ccagctcgtt cagcttttcc agcaacagca gcgactgatc cttaatgaat ttagccatat
     2341 tgagggcagt tttttcctgc ttcgtcatgt tctccattcc cttcggtaag ttctcagcta
     2401 aaccagccgg accagaagcg ttttttcttc tgtgtcagtt ctgttgtcaa tctgtccact
     2461 tcggcctgga gtttccggcg ctcatcatcg tgctggcggc gttcttcttc ccgggatgtt
     2521 ttatcttcca gcatcagggt gacagcctgc cggagatcgg ccagttcctt ttgcattgcc
     2581 agaagctgtt cctgtgggaa atttttttgt gtattttcac ggtgtgaaat gccgggaatt
     2641 tgtttctcag ggatattcac atttccgtat acacggatca gttctgacac atccacgaca
     2701 gggttgtttt tcccgtcacg tgacacggta acctttccct gttttacatg attgtatagc
     2761 gttcttctgg taatgcccgc ggcttgtgct gcctgtgaaa ggttgagcaa tgttttcgcc
     2821 actgtatacc cctgatttta tcgggtgtga aaggtgtgta atttctcacg gtaaatgtat
     2881 cgctgaattt tacccgtttt tccgttcagg atgtggtttt ctgacgcgct gcgcttgtca
     2941 aaccggcgac ggccacgcaa tgaattgcgc ccccgtcatc ggttattaga ggctgtctgc
     3001 ccccggatac ccggattctt tctgatgcct gacggtgacg atcacaatct ggtccatttc
     3061 ccggtcgtgg cggtagagca tcacatagcc actgtcgcca aacccgatca ccagttcctt
     3121 atattccagc ggcagaaacg gcaccgggcg gccaatgtct ggcaatgttt tcagttgctg
     3181 gatagcccgg acgatcacct cacctccttt tctggctgcc agccgatttt tggttttgag
     3241 aaagtcctga aggcgttgta aatcgcgctg tgccagcgcg gaaatcgtta cctgtggcat
     3301 ggcggagcat cctgctcgtt ctcagttccc caggtactta tccaggcttc cgcttcttcc
     3361 gcagtaaggt gcagacccgt ttcctgatag tgctgccatg cggcctgtcc gtcccgcagg
     3421 tactggctgc gcttctcttc ccggtcgatg tattcggtga ttgcctccag cattagcgcg
     3481 tgcgctgagc ggtgccggtc atcggccagc gttttcagcc ggtccttaag ttcttcatcg
     3541 agcctgatgg atgttgctga tgacataaaa gcccctttgt agtcatatgt attacacatg
     3601 actactttaa tccgtggcgg cgctatttgc cagaaatacg gcttttgctg cccatttttc
     3661 agcttcgctt ccactaatca gggctgctac aggctcactc atcatccagg actattgcag
     3721 cctgcgggtt aaaggacctg ttactggcgg caaccatcat cattgagctt ttgggtggtg
     3781 actggtcgcg gatcctgtaa gccgtcattt ttcagactga attcaaaatt tataacctgt
     3841 cgcattgccg ggtgccctgc gggatatagc cagtgcctca ttgccgtcag cgtttcccga
     3901 aaaaaaaggt gacagccggt aagcgacacg ttgtccactc gtccatcgtc cagaatatca
     3961 aacgtggcgg tcacctgacc ttcgatgcgt tttgctgcgg cctgtaccgg gtaggttggc
     4021 tgcagtgtgg ttacgggttt tagtggtatc gcatgtaccg gagtgaggaa catggccgcc
     4081 agcactgtca gagtaaaagc tttcatggtc tgaaatccag aggggcgttt ttgaatattt
     4141 gccaggtatt ttcatccggt gccggcggaa ttttggcgtg ggtaacggct gatattgctg
     4201 ctttacagag tttcggatct cctccttcag caatggcgct gttcagagtg ccatcacgtt
     4261 gtatggatat ccgcacggtg cacatttttc ccgcataggc ctctggttca aacatctgca
     4321 tttcaacagc ggtcttgatg gccgcagcgt agctactgat tttagatgac ttatccagag
     4381 tataaatttt cggcggctgg gtggtggaat gcttacaggc cgtcaggagt aaggggctgc
     4441 acaatgccag caggataaag ggtattaaac gcatcgggtt gatgacctgt cagtaaaaaa
     4501 tgttacatgt atatttgtca ctctttacgg ccacgttcat ttacataaac acggcttatc
     4561 gtatcgtttt tacaccaaac ggcccgccat gcagttgcgt gcataggcat gcagcgggtt
     4621 acaatttacc gggcgcgttt ccggcggttt tccgggtggt ttgttgcctg ttttatcccg
     4681 tagccgccgg aaacgccctc agaccgtctg agcggtgcgg gcaatgcgac gttatggtaa
     4741 atacccctta ccggcggcgc tgtggctgcc aggtgaaggt ggtttccgta gcctcgtatt
     4801 tctcccgacg catttcctgc gcccgcctgg cttcacgttc gtcctgaatg gcacgaagca
     4861 ccctttcccg cacctgttgt tttaccgcct ttggcagcgt tttgttccct ttcaggccca
     4921 tggccagtgc ctgagcgaca aaggcgtccg ccaggcgctt ttcttcagcg ggagtcagtt
     4981 tcaggtgcgt catagtggcc tccgggcgtc tcaggtttcc cgaacagttt aaccgcaggt
     5041 cctctcctca tgccagttag ctttcagatg tgtctatacc atctgaaatg taagtgtgat
     5101 gaatgtctta aataataaaa tttgttgcca tgagaaattt tcgggaaaag acgcgatctg
     5161 cttacaggtg gctccgggcg cttttttgtg cctgaagatg agaactaatc tcatatattg
     5221 acacctgaaa acgggaggag taaggtaata attatactgt gtatatgtac agtatgttgg
     5281 ctttaattta ttgttttaaa tgtcaaagag gaaaacggta tgagtggtgg agacggtcga
     5341 ggtccgggta attcaggtct gggacataat ggtggtcagg ccagtgggaa tgtgaacggt
     5401 acgtctggta aaggtggccc ttcatcaggt ggtggtacgg atccaaacag cgggccgggc
     5461 tggggtacga cgcatacgcc gaatggagat attcataact ataatccggg ggagtttggt
     5521 aacggcggga gtaaacccgg tggtaatggt ggtaacagcg gcaatcatag cggtagctct
     5581 ggtggcggac agtcttcggc cactgcgatg gccttcggtc tgcctgccct ggctactccg
     5641 ggggctgaag gactggcttt atcagtttcc ggcgatgcgt tgtcggccgc cgttgctgat
     5701 gtgctggctg ccctgaaggg gccgtttaag tttggtctgt gggggattgc gatctacggt
     5761 gtgctgcctt ctgagattgc aaaagatgat ccgaaaatga tgtcaaaaat tatgacgtca
     5821 ttaccggccg atgcggtgac ggagactccg gcaagtactt taccactgga ccaggcgacg
     5881 gttcgtgtca gacaacgggt tgtggatgtg gtgaaggatg agcggcagca tattgcggtt
     5941 gtcgcaggtc ggccaatgag tgtccctgtg gtggatgcga aaccgacaaa acgtccgggg
     6001 gtattcagtg tgtcgattcc gggtctcccg tctctgcagg tgagcgtacc gaaaggtgtt
     6061 ccgacagcga aagccccgcc aaaaggcatt gttgctgaaa aaggtgattc acgtccggct
     6121 ggttttacag ccggtggtaa ctcccgtgag gccgttattc gtttcccgaa agagaccgga
     6181 cagaagccgg tttatgtgtc ggtgacagat gttcttaccc cggcacaggt aaaacagcgt
     6241 caggaggaag aaaagcgtcg ccagcaggca tgggacgccg ctcatccgga agaggggctg
     6301 aaaagagact atgataaagc gaaagctgag ctggatgccg aagataaaaa tattgcgacc
     6361 ttaaacagcc gcattgcatc gacagagaag gcgctccccg gtgcaagggc tgctgttcag
     6421 gaagccgata aaaaggtaaa agaggcagag gcaaataaag atgattttgt gacttataac
     6481 ccgcctcatg aatatggctc cgggtggcag gatcaggttc gctatcttga taaggatatt
     6541 cagaatcaga atgagaaatt aaaagcggct caggcatctt taaacgcaat gaatgaatcc
     6601 ttatccagag ataaggctgc acttaccggg gcgatggaga gccggaaaca aaaggagaaa
     6661 aaagcgaagg atgcagaaaa taagttaaat gaggaaaaga acaagcctcg caaggggact
     6721 aaagactacg gccatgatta tcatccagta cctaaaacag aagacataaa gggacttgga
     6781 gagttaaagc gaggtgatcc taaaacacct aaacaaggtg gtggtggtaa gcgggcccga
     6841 tggtatggcg ataaaaaacg taaaatttat gaatgggatt cccagcacgg tgagcttgaa
     6901 gggtatcgtg ccagtgatgg cgaacaccta ggcgcatttg atcccaaaac gggtaagcag
     6961 gttaaagggc cagatccaaa acggaacatt aaaaaatatc tttgagaggt aaatatgggg
     7021 cttaaattac gtttgacatg gtttgataag aaaactgaag agtttaaagg ggaagagtat
     7081 tctaaagact ttggtgacga tggttctgtc attgaaagct tgggaatgcc tttaaaggat
     7141 aatattaata atggtgagtt tgatttgaag aaagaatggg ttcctttact acagccattt
     7201 tttaaaaatc aaataaaacc tgatgtggat aattgttata tagcttttga ttatcgggac
     7261 ggtaactggt aacaaaaaat tatcaaggat gatttgaaat actgacttcc ggtgttcagg
     7321 gatgaatgcc ggagtcttta aatacaggga aagtatatga aaaaggtaaa gactattttt
     7381 ctttttatat tgattgttgc tggctttctt ctggccgcat gtcaggcaaa ttatatccgg
     7441 gatgttcagg gcggaacggt ggcaccatcg tcctcctctg aactgacggg gatcgcggtt
     7501 cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atcttttgcc
     7561 ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa
     7621 ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg
     7681 tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg
     7741 cgaactccca gttaccaatg gctgctgcca gtggcgtttt gcgtgctttc ccgggttgga
     7801 ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacgggga gttcttgctt
     7861 acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagtgtg tgagctatga
     7921 gaaagcgcca cacttcccgc aagggagaac ggcggacagg tatccggtaa acggcagggt
     7981 cggaacagga gagcgcaaga gggagcgacc taccggaaac ggtggggatc tttaagtcct
     8041 gtcgggtttc gcccgtactg tcagattcat ggttgagcct cacggctccc acagatgcac
     8101 cgaaaaagcg tctgtttatg tgaactctgg caggagggcg gagcctatgg aaaaacgcca
     8161 ccggcgcggc cctgccgttt tgcctcacat gttagtcccc tgcttatcca cggaatctgt
     8221 gggtaacttt gtatgtgtcc gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc
     8281 gagtgagcga ggaggcgcaa tttccccggc acctgcaaga cggtcaccgg acgcgctgat
     8341 gtgggatatt gtcccctgat taagtctttc tattccctga cgatcaatta tcaggatagg
     8401 tgaaccatgt cggtttttcg ctgaacattt tacgttaacc tatctctgtt gcttttttct
     8461 ccttgcggag aaatgcaagt catccccgct ggggcgtggc tgacttcagg ggaaaagacc
     8521 ttgcttacgc tggctggacg ccaccgacgc acggtctttt cccctctttc tggctctgtc
     8581 cggctggacg ccggccacgc cagaaattca cccggccccc gtcaggctca cgcctgacca
     8641 cccacaaaaa tccacggtgc tggacgcacc tgacgaagca gcaaagctgc tcctgatttt
     8701 gcgggtggtc agccgctcgc tatggcgaac tctgacgggg gccggaacac cggaaaagac
     8761 cgcactgggg tgccgccttt tcctctcaca cgctggggcg tgctcgttgt tcctccgggg
     8821 ctgcgccttt tccgcatggg gcggaaagag gctggcctcc gcggtgaaag ctgaacgcaa
     8881 tcaccacgga cgtgaccgcc gccgccatgc cttggggcat gtccgcggct tgtgcagcgg
     8941 gggctgcgca tcacgccggt aaacccgtcg tctgaaggag agagattgca tgtttaatac
     9001 ggattcgctt gcctggcagt ggcatatccc ggagctggcc cggcagattt acccgtgggt
     9061 cagctggctg taccgggagt attacagtct ggccggtttt tttctggtta tcggcctgtt
     9121 tttcggtctg gctgaactct gggccggccg aatgcgtttt gtggggctga gccataaggc
     9181 gcggcgggag tatgccgatc gctataagcg cgataataac cggactgtgc cattttcggt
     9241 gctggcctcg ctggattcac ccgggcggat ggtcagggca cggcgacatg tctggaacta
     9301 cgcgtactgg ctgaccggcg ctgccggtct ggcccttctg ggggggctgg ttgtcgccgt
     9361 gtttatcgag agctggggag aaaccgtcct gcgcccggac ctctatgcga acgcagatta
     9421 ttacctgctg cagccggcta acctgttcgg gatagcctgg tgtgccggag ggcttgtcgc
     9481 cggcgtcctt ctggcgcggc tgctggcgtt cctgctgctt gatggtcatt ttgcggccgc
     9541 tgacgaggcg gtgaacgcga aactgaatca ggagagccgg cgcagcagcc agcggaccgg
     9601 agaaatgaca gacgtccgcc acctgcattt cggggagccg gtaccggtta acgcgctggc
     9661 cgatttttcc acggagcagg cccggaaaca gcaggcggtg tttctgggta aggatgagca
     9721 gggccagccg gtgctggtcc ctcgtgatac ctggcgcaag accaatattc agatcctcgg
     9781 cctgcccggg agcggcaaga gcgcgatggc caccaacgcg ctcattcgct gcgtacgcga
     9841 tttcggggac gctgtggtgt acttcgaccc caaaggggac gcgtgggcgc cgcacgtctt
     9901 ccgggcgcac tgcccggact tcaccctgct ggatctgcgg ccggggaaac cggcgcagct
     9961 gaatctgttc cgcgatctcg accagtacgc cctgaagaat ctgctggttg ccgggtttaa
    10021 cctgagtgag accggcgacg tggcggacca ctaccgcatc
//
