LOCUS       NZ_CP136825            10060 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Citrobacter koseri strain K1818 plasmid pK1118-KPC, complete
            sequence.
ACCESSION   NZ_CP136825
VERSION     NZ_CP136825.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37851225
            Assembly: GCF_032945095.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K1818 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:29:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,580
            CDSs (total)                      :: 4,466
            Genes (coding)                    :: 4,405
            CDSs (with protein)               :: 4,405
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 23 of 61
            Pseudo Genes (incomplete)         :: 41 of 61
            Pseudo Genes (internal stop)      :: 14 of 61
            Pseudo Genes (multiple problems)  :: 13 of 61
            Pseudo Genes (short protein)      :: 1 of 61
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136825.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K1818"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK1118-KPC"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2018-01-30"
                     /collected_by="Jiayao Yao"
     gene            join(8838..10060,1..463)
                     /locus_tag="R1221_RS22495"
                     /old_locus_tag="R1221_22495"
     CDS             join(8838..10060,1..463)
                     /locus_tag="R1221_RS22495"
                     /old_locus_tag="R1221_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            473..730
                     /locus_tag="R1221_RS22500"
                     /old_locus_tag="R1221_22500"
     CDS             473..730
                     /locus_tag="R1221_RS22500"
                     /old_locus_tag="R1221_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            815..964
                     /locus_tag="R1221_RS22505"
                     /old_locus_tag="R1221_22505"
     CDS             815..964
                     /locus_tag="R1221_RS22505"
                     /old_locus_tag="R1221_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2448..4412
                     /locus_tag="R1221_RS22510"
                     /old_locus_tag="R1221_22510"
     CDS             2448..4412
                     /locus_tag="R1221_RS22510"
                     /old_locus_tag="R1221_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4412..5143
                     /gene="mobC"
                     /locus_tag="R1221_RS22515"
                     /old_locus_tag="R1221_22515"
     CDS             4412..5143
                     /gene="mobC"
                     /locus_tag="R1221_RS22515"
                     /old_locus_tag="R1221_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5150..5680
                     /locus_tag="R1221_RS22520"
                     /old_locus_tag="R1221_22520"
     CDS             5150..5680
                     /locus_tag="R1221_RS22520"
                     /old_locus_tag="R1221_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5707..5886)
                     /locus_tag="R1221_RS22525"
                     /old_locus_tag="R1221_22525"
     CDS             complement(5707..5886)
                     /locus_tag="R1221_RS22525"
                     /old_locus_tag="R1221_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5912..6340)
                     /locus_tag="R1221_RS22530"
                     /old_locus_tag="R1221_22530"
     CDS             complement(5912..6340)
                     /locus_tag="R1221_RS22530"
                     /old_locus_tag="R1221_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6504..6818)
                     /locus_tag="R1221_RS22535"
                     /old_locus_tag="R1221_22535"
     CDS             complement(6504..6818)
                     /locus_tag="R1221_RS22535"
                     /old_locus_tag="R1221_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6806..7084)
                     /locus_tag="R1221_RS22540"
                     /old_locus_tag="R1221_22540"
     CDS             complement(6806..7084)
                     /locus_tag="R1221_RS22540"
                     /old_locus_tag="R1221_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7259..7624)
                     /locus_tag="R1221_RS22545"
                     /old_locus_tag="R1221_22545"
     CDS             complement(7259..7624)
                     /locus_tag="R1221_RS22545"
                     /old_locus_tag="R1221_22545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7621..7833)
                     /locus_tag="R1221_RS22550"
                     /old_locus_tag="R1221_22550"
     CDS             complement(7621..7833)
                     /locus_tag="R1221_RS22550"
                     /old_locus_tag="R1221_22550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="WP_250203192.1"
                     /translation="MFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVT
                     HAKIPPAPDENTWQIFKNAPLDFRP"
                     /name="cell envelope integrity TolA C-terminal
                     domain-containing protein"
     gene            complement(8266..8511)
                     /locus_tag="R1221_RS22555"
                     /old_locus_tag="R1221_22555"
     CDS             complement(8266..8511)
                     /locus_tag="R1221_RS22555"
                     /old_locus_tag="R1221_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             3838..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1068..1646)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4413..5140
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gaatcagaat gagaaattaa aagcggctca ggcatcttta aacgcaatga atgaatcctt
       61 atccagagat aaggctgcac ttaccggggc gatggagagc cggaaacaaa aggagaaaaa
      121 agcgaaggat gcagaaaata agttaaatga ggaaaagaac aagcctcgca aggggactaa
      181 agactacggc catgattatc atccagtacc taaaacagaa gacataaagg gacttggaga
      241 gttaaagcga ggtgatccta aaacacctaa acaaggtggt ggtggtaagc gggcccgatg
      301 gtatggcgat aaaaaacgta aaatttatga atgggattcc cagcacggtg agcttgaagg
      361 gtatcgtgcc agtgatggcg aacacctagg cgcatttgat cccaaaacgg gtaagcaggt
      421 taaagggcca gatccaaaac ggaacattaa aaaatatctt tgagaggtaa atatggggct
      481 taaattacgt ttgacatggt ttgataagaa aactgaagag tttaaagggg aagagtattc
      541 taaagacttt ggtgacgatg gttctgtcat tgaaagcttg ggaatgcctt taaaggataa
      601 tattaataat ggtgagtttg atttgaagaa agaatgggtt cctttactac agccattttt
      661 taaaaatcaa ataaaacctg atgtggataa ttgttatata gcttttgatt atcgggacgg
      721 taactggtaa caaaaaatta tcaaggatga tttgaaatac tgacttccgg tgttcaggga
      781 tgaatgccgg agtctttaaa tacagggaaa gtatatgaaa aaggtaaaga ctatttttct
      841 ttttatattg attgttgctg gctttcttct ggccgcatgt caggcaaatt atatccggga
      901 tgttcagggc ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga tcgcggttca
      961 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat cttttgccct
     1021 gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact
     1081 gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta
     1141 gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg
     1201 aactcccagt taccaatggc tgctgccagt ggcgttttgc gtgctttccc gggttggact
     1261 caagtgaaca gttaccggat aaggcgcagc agtcgggctg aacggggagt tcttgcttac
     1321 agcccagctt ggagcgaacg acctacaccg agccgagata ccagtgtgtg agctatgaga
     1381 aagcgccaca cttcccgcaa gggagaacgg cggacaggta tccggtaaac ggcagggtcg
     1441 gaacaggaga gcgcaagagg gagcgaccta ccggaaacgg tggggatctt taagtcctgt
     1501 cgggtttcgc ccgtactgtc agattcatgg ttgagcctca cggctcccac agatgcaccg
     1561 aaaaagcgtc tgtttatgtg aactctggca ggagggcgga gcctatggaa aaacgccacc
     1621 ggcgcggccc tgccgttttg cctcacatgt tagtcccctg cttatccacg gaatctgtgg
     1681 gtaactttgt atgtgtccgc agcgcccgcc gcagtctcac gcccggagcg tagcgaccga
     1741 gtgagcgagg aggcgcaatt tccccggcac ctgcaagacg gtcaccggac gcgctgatgt
     1801 gggatattgt cccctgatta agtctttcta ttccctgacg atcaattatc aggataggtg
     1861 aaccatgtcg gtttttcgct gaacatttta cgttaaccta tctctgttgc ttttttctcc
     1921 ttgcggagaa atgcaagtca tccccgctgg ggcgtggctg acttcagggg aaaagacctt
     1981 gcttacgctg gctggacgcc accgacgcac ggtcttttcc cctctttctg gctctgtccg
     2041 gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg cctgaccacc
     2101 cacaaaaatc cacggtgctg gacgcacctg acgaagcagc aaagctgctc ctgattttgc
     2161 gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg
     2221 cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct
     2281 gcgccttttc cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct gaacgcaatc
     2341 accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg tgcagcgggg
     2401 gctgcgcatc acgccggtaa acccgtcgtc tgaaggagag agattgcatg tttaatacgg
     2461 attcgcttgc ctggcagtgg catatcccgg agctggcccg gcagatttac ccgtgggtca
     2521 gctggctgta ccgggagtat tacagtctgg ccggtttttt tctggttatc ggcctgtttt
     2581 tcggtctggc tgaactctgg gccggccgaa tgcgttttgt ggggctgagc cataaggcgc
     2641 ggcgggagta tgccgatcgc tataagcgcg ataataaccg gactgtgcca ttttcggtgc
     2701 tggcctcgct ggattcaccc gggcggatgg tcagggcacg gcgacatgtc tggaactacg
     2761 cgtactggct gaccggcgct gccggtctgg cccttctggg ggggctggtt gtcgccgtgt
     2821 ttatcgagag ctggggagaa accgtcctgc gcccggacct ctatgcgaac gcagattatt
     2881 acctgctgca gccggctaac ctgttcggga tagcctggtg tgccggaggg cttgtcgccg
     2941 gcgtccttct ggcgcggctg ctggcgttcc tgctgcttga tggtcatttt gcggccgctg
     3001 acgaggcggt gaacgcgaaa ctgaatcagg agagccggcg cagcagccag cggaccggag
     3061 aaatgacaga cgtccgccac ctgcatttcg gggagccggt accggttaac gcgctggccg
     3121 atttttccac ggagcaggcc cggaaacagc aggcggtgtt tctgggtaag gatgagcagg
     3181 gccagccggt gctggtccct cgtgatacct ggcgcaagac caatattcag atcctcggcc
     3241 tgcccgggag cggcaagagc gcgatggcca ccaacgcgct cattcgctgc gtacgcgatt
     3301 tcggggacgc tgtggtgtac ttcgacccca aaggggacgc gtgggcgccg cacgtcttcc
     3361 gggcgcactg cccggacttc accctgctgg atctgcggcc ggggaaaccg gcgcagctga
     3421 atctgttccg cgatctcgac cagtacgccc tgaagaatct gctggttgcc gggtttaacc
     3481 tgagtgagac cggcgacgtg gcggaccact accgcatcag cgagcagaaa gcggcgaagc
     3541 tgattgcgga gcagttcccg cagggggcaa acattcagca ggtgctggcg gcggcgtacg
     3601 cgttgccgga ggccctgaaa aaggacgtga aggggctgat caccaagctg gagaacgtgg
     3661 ccgacctgag cgtcctgcag acggacagcg gtatcgatgt ggcggggata atcaacggcg
     3721 gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg gtgcagaaga
     3781 tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga tgagttccgg aggtggccgc
     3841 acgcgagcat catgctcgat gagattaaat atctgctgtc gaagtacgtg ctgaacgcgc
     3901 tggggacgct gcgcagccgg gactgcaacc tgctgctcgc gcaccagtcg ctgggcgact
     3961 tcggtcagtg cgggcaggat ctgcccgctg attttgtgaa aaccacggta ctggataaca
     4021 cgccgatccg ctggttctat cgcgcggcca gccaggagtc cgcgcagtgg gcggcgggcc
     4081 agaccgggga gatccgggtg gacgtggagc gccgccgggc cagccgggag gcggggaacg
     4141 tggagcacat cagcggcgac agcttcatcc agaaggaggc gcggccgctg tttgacgtga
     4201 acaccctgca acacctgcct gacgggttcg cggtgatgac cgggctgggc gtggcgcggc
     4261 tggcgttcag cagcccgctg cgggttgagc gccgggaaat ttcgctgaaa accttcccgg
     4321 tgctggcgaa aacggacccg ctggcggagt acctgcctcc ggccgacggc ggcggaccaa
     4381 aagcggatga tgattttgcg gggctttact gatgacactg gagcagtaca acgtcagcca
     4441 cgcgaagcgg caggcgcgga acgcggagaa aacacggctg accctgcgct ggctgcggga
     4501 agagctgtgt tccacggcgg agctggttgc gcgacggctg gggattgcgg cggtacagcc
     4561 ggtttaccgc ttcctggaca gtctggtggc gaaagggctg ctggtccggg cgaaataccc
     4621 ggttgacggc cgtcaggtca gcgtgtgggg gctgacgccg cacggggtgg cgttctcgtt
     4681 cgacgaggac gagccgctga cggacgttat tccgtttcag ccgtcaaggg tgtcggcggc
     4741 gcagctgccg caccggctgg ccgtgcagtc cctgcgcctc tccatggagg gccggggggc
     4801 caccggctgg cggtacctgc accgtatggc cctgaaaggg atgaaagtgc cggacgcgct
     4861 ggcggagctg gacggcagaa cggtggcatt tgaggtagag cgcaccgtca agagccgcag
     4921 gcggtatcag gaagtggtgt ccgggtatct gttcaaccgc cgggcgaacg gcatcgatga
     4981 aatctggtac atctgccctg accggccgac gcttgtccgg gtacagcggg cgattctggc
     5041 ggtggatgaa atcgtgaatc cacagaccgg cgaggcccgc aaaacggtgg agctggaccg
     5101 ggagaggctg ttcgcgtgct ttaaatttat gacaacggag tgagggatta tggcgctgta
     5161 catggtattt attgaggacg tggaagacaa agacggcgag cgctgcatta tgccaaccag
     5221 cgagcatctg gaagacatgg tggtgctcgc ctggcagacc ggggcgtatg ttttctgttt
     5281 tgtcgaccgg gcccagcatc tggcgatgat gaaatccatc gggatgtatg acgagcggct
     5341 caacagcgac gaaaaagcca cgtacttttt caccggtgaa aatccgcaat gggaacggga
     5401 caaacccaca ggaatgaagc tgtgtgatta cctctatcga aaggtggagc ggttcgccat
     5461 cgagaacggt gtgcaggtgg ttgatgccgg gaagtgctca ccggagcaga ttaacgcgat
     5521 gattgaggtg gtcagcgagc ggcacgggga aagcgcggtg aacgagaagc tgaaagcgga
     5581 gctgatgcgg gaatataaca gccgctattt taacgtgccg gtgcctgaac tgccggagga
     5641 caaacccgcc cgccggtggt ggccgttcgg caaaaaataa cgccagcacc ggcatggccg
     5701 gtgctgttat tcttccccga gcttatcccg catcacctgc cagagtgttt ccgcatcctc
     5761 gtgaagccgt tcgcacatgt cggccggggt atccatgtcc agctcgttca gcttttccag
     5821 caacagcagc gactgatcct taatgaattt agccatattg agggcagttt tttcctgctt
     5881 cgtcatgttc tccattccct tcggtaagtt ctcagctaaa ccagccggac cagaagcgtt
     5941 ttttcttctg tgtcagttct gttgtcaatc tgtccacttc ggcctggagt ttccggcgct
     6001 catcatcgtg ctggcggcgt tcttcttccc gggatgtttt atcttccagc atcagggtga
     6061 cagcctgccg gagatcggcc agttcctttt gcattgccag aagctgttcc tgtgggaaat
     6121 ttttttgtgt attttcacgg tgtgaaatgc cgggaatttg tttctcaggg atattcacat
     6181 ttccgtatac acggatcagt tctgacacat ccacgacagg gttgtttttc ccgtcacgtg
     6241 acacggtaac ctttccctgt tttacatgat tgtatagcgt tcttctggta atgcccgcgg
     6301 cttgtgctgc ctgtgaaagg ttgagcaatg ttttcgccac tgtatacccc tgattttatc
     6361 gggtgtgaaa ggtgtgtaat ttctcacggt aaatgtatcg ctgaatttta cccgtttttc
     6421 cgttcaggat gtggttttct gacgcgctgc gcttgtcaaa ccggcgacgg ccacgcaatg
     6481 aattgcgccc ccgtcatcgg ttattagagg ctgtctgccc ccggataccc ggattctttc
     6541 tgatgcctga cggtgacgat cacaatctgg tccatttccc ggtcgtggcg gtagagcatc
     6601 acatagccac tgtcgccaaa cccgatcacc agttccttat attccagcgg cagaaacggc
     6661 accgggcggc caatgtctgg caatgttttc agttgctgga tagcccggac gatcacctca
     6721 cctccttttc tggctgccag ccgatttttg gttttgagaa agtcctgaag gcgttgtaaa
     6781 tcgcgctgtg ccagcgcgga aatcgttacc tgtggcatgg cggagcatcc tgctcgttct
     6841 cagttcccca ggtacttatc caggcttccg cttcttccgc agtaaggtgc agacccgttt
     6901 cctgatagtg ctgccatgcg gcctgtccgt cccgcaggta ctggctgcgc ttctcttccc
     6961 ggtcgatgta ttcggtgatt gcctccagca ttagcgcgtg cgctgagcgg tgccggtcat
     7021 cggccagcgt tttcagccgg tccttaagtt cttcatcgag cctgatggat gttgctgatg
     7081 acataaaagc ccctttgtag tcatatgtat tacacatgac tactttaatc cgtggcggcg
     7141 ctatttgcca gaaatacggc ttttgctgcc catttttcag cttcgcttcc actaatcagg
     7201 gctgctacag gctcactcat catccaggac tattgcagcc tgcgggttaa aggacctgtt
     7261 actggcggca accatcatca ttgagctttt gggtggtgac tggtcgcgga tcctgtaagc
     7321 cgtcattttt cagactgaat tcaaaattta taacctgtcg cattgccggg tgccctgcgg
     7381 gatatagcca gtgcctcatt gccgtcagcg tttcccgaaa aaaaaggtga cagccggtaa
     7441 gcgacacgtt gtccactcgt ccatcgtcca gaatatcaaa cgtggcggtc acctgacctt
     7501 cgatgcgttt tgctgcggcc tgtaccgggt aggttggctg cagtgtggtt acgggtttta
     7561 gtggtatcgc atgtaccgga gtgaggaaca tggccgccag cactgtcaga gtaaaagctt
     7621 tcatggtctg aaatccagag gggcgttttt gaatatttgc caggtatttt catccggtgc
     7681 cggcggaatt ttggcgtggg taacggctga tattgctgct ttacagagtt tcggatctcc
     7741 tccttcagca atggcgctgt tcagagtgcc atcacgttgt atggatatcc gcacggtgca
     7801 catttttccc gcataggcct ctggttcaaa catctgcatt tcaacagcgg tcttgatggc
     7861 cgcagcgtag ctactgattt tagatgactt atccagagta taaattttcg gcggctgggt
     7921 ggtggaatgc ttacaggccg tcaggagtaa ggggctgcac aatgccagca ggataaaggg
     7981 tattaaacgc atcgggttga tgacctgtca gtaaaaaatg ttacatgtat atttgtcact
     8041 ctttacggcc acgttcattt acataaacac ggcttatcgt atcgttttta caccaaacgg
     8101 cccgccatgc agttgcgtgc ataggcatgc agcgggttac aatttaccgg gcgcgtttcc
     8161 ggcggttttc cgggtggttt gttgcctgtt ttatcccgta gccgccggaa acgccctcag
     8221 accgtctgag cggtgcgggc aatgcgacgt tatggtaaat accccttacc ggcggcgctg
     8281 tggctgccag gtgaaggtgg tttccgtagc ctcgtatttc tcccgacgca tttcctgcgc
     8341 ccgcctggct tcacgttcgt cctgaatggc acgaagcacc ctttcccgca cctgttgttt
     8401 taccgccttt ggcagcgttt tgttcccttt caggcccatg gccagtgcct gagcgacaaa
     8461 ggcgtccgcc aggcgctttt cttcagcggg agtcagtttc aggtgcgtca tagtggcctc
     8521 cgggcgtctc aggtttcccg aacagtttaa ccgcaggtcc tctcctcatg ccagttagct
     8581 ttcagatgtg tctataccat ctgaaatgta agtgtgatga atgtcttaaa taataaaatt
     8641 tgttgccatg agaaattttc gggaaaagac gcgatctgct tacaggtggc tccgggcgct
     8701 tttttgtgcc tgaagatgag aactaatctc atatattgac acctgaaaac gggaggagta
     8761 aggtaataat tatactgtgt atatgtacag tatgttggct ttaatttatt gttttaaatg
     8821 tcaaagagga aaacggtatg agtggtggag acggtcgagg tccgggtaat tcaggtctgg
     8881 gacataatgg tggtcaggcc agtgggaatg tgaacggtac gtctggtaaa ggtggccctt
     8941 catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg catacgccga
     9001 atggagatat tcataactat aatccggggg agtttggtaa cggcgggagt aaacccggtg
     9061 gtaatggtgg taacagcggc aatcatagcg gtagctctgg tggcggacag tcttcggcca
     9121 ctgcgatggc cttcggtctg cctgccctgg ctactccggg ggctgaagga ctggctttat
     9181 cagtttccgg cgatgcgttg tcggccgccg ttgctgatgt gctggctgcc ctgaaggggc
     9241 cgtttaagtt tggtctgtgg gggattgcga tctacggtgt gctgccttct gagattgcaa
     9301 aagatgatcc gaaaatgatg tcaaaaatta tgacgtcatt accggccgat gcggtgacgg
     9361 agactccggc aagtacttta ccactggacc aggcgacggt tcgtgtcaga caacgggttg
     9421 tggatgtggt gaaggatgag cggcagcata ttgcggttgt cgcaggtcgg ccaatgagtg
     9481 tccctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg tcgattccgg
     9541 gtctcccgtc tctgcaggtg agcgtaccga aaggtgttcc gacagcgaaa gccccgccaa
     9601 aaggcattgt tgctgaaaaa ggtgattcac gtccggctgg ttttacagcc ggtggtaact
     9661 cccgtgaggc cgttattcgt ttcccgaaag agaccggaca gaagccggtt tatgtgtcgg
     9721 tgacagatgt tcttaccccg gcacaggtaa aacagcgtca ggaggaagaa aagcgtcgcc
     9781 agcaggcatg ggacgccgct catccggaag aggggctgaa aagagactat gataaagcga
     9841 aagctgagct ggatgccgaa gataaaaata ttgcgacctt aaacagccgc attgcatcga
     9901 cagagaaggc gctccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag
     9961 aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg
    10021 ggtggcagga tcaggttcgc tatcttgata aggatattca
//
