LOCUS       NZ_CP136811            10060 bp    DNA     circular CON 26-OCT-2023
DEFINITION  Citrobacter koseri strain K1721 plasmid pK1721-2, complete sequence.
ACCESSION   NZ_CP136811
VERSION     NZ_CP136811.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37824052
            Assembly: GCF_032939275.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri
  ORGANISM  Citrobacter koseri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Citrobacter koseri K1721 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/26/2023 13:47:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,605
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,413
            CDSs (with protein)               :: 4,413
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 25 of 76
            Pseudo Genes (incomplete)         :: 50 of 76
            Pseudo Genes (internal stop)      :: 15 of 76
            Pseudo Genes (multiple problems)  :: 12 of 76
            Pseudo Genes (short protein)      :: 1 of 76
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136811.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Citrobacter koseri"
                     /mol_type="genomic DNA"
                     /strain="K1721"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:545"
                     /plasmid="pK1721-2"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2021-07-30"
                     /collected_by="Jiayao Yao"
     gene            join(10002..10060,1..121)
                     /locus_tag="R1158_RS22480"
                     /old_locus_tag="R1158_22480"
     CDS             join(10002..10060,1..121)
                     /locus_tag="R1158_RS22480"
                     /old_locus_tag="R1158_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(148..678)
                     /locus_tag="R1158_RS22485"
                     /old_locus_tag="R1158_22485"
     CDS             complement(148..678)
                     /locus_tag="R1158_RS22485"
                     /old_locus_tag="R1158_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(685..1416)
                     /gene="mobC"
                     /locus_tag="R1158_RS22490"
                     /old_locus_tag="R1158_22490"
     CDS             complement(685..1416)
                     /gene="mobC"
                     /locus_tag="R1158_RS22490"
                     /old_locus_tag="R1158_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1416..3380)
                     /locus_tag="R1158_RS22495"
                     /old_locus_tag="R1158_22495"
     CDS             complement(1416..3380)
                     /locus_tag="R1158_RS22495"
                     /old_locus_tag="R1158_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4864..5013)
                     /locus_tag="R1158_RS22500"
                     /old_locus_tag="R1158_22500"
     CDS             complement(4864..5013)
                     /locus_tag="R1158_RS22500"
                     /old_locus_tag="R1158_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5098..5355)
                     /locus_tag="R1158_RS22505"
                     /old_locus_tag="R1158_22505"
     CDS             complement(5098..5355)
                     /locus_tag="R1158_RS22505"
                     /old_locus_tag="R1158_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5365..7050)
                     /locus_tag="R1158_RS22510"
                     /old_locus_tag="R1158_22510"
     CDS             complement(5365..7050)
                     /locus_tag="R1158_RS22510"
                     /old_locus_tag="R1158_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7377..7622
                     /locus_tag="R1158_RS22515"
                     /old_locus_tag="R1158_22515"
     CDS             7377..7622
                     /locus_tag="R1158_RS22515"
                     /old_locus_tag="R1158_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8055..8267
                     /locus_tag="R1158_RS22520"
                     /old_locus_tag="R1158_22520"
     CDS             8055..8267
                     /locus_tag="R1158_RS22520"
                     /old_locus_tag="R1158_22520"
                     /inference="COORDINATES: protein motif:HMM:NF018252.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="WP_250203192.1"
                     /translation="MFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVT
                     HAKIPPAPDENTWQIFKNAPLDFRP"
                     /name="cell envelope integrity TolA C-terminal
                     domain-containing protein"
     gene            8264..8629
                     /locus_tag="R1158_RS22525"
                     /old_locus_tag="R1158_22525"
     CDS             8264..8629
                     /locus_tag="R1158_RS22525"
                     /old_locus_tag="R1158_22525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            8804..9082
                     /locus_tag="R1158_RS22530"
                     /old_locus_tag="R1158_22530"
     CDS             8804..9082
                     /locus_tag="R1158_RS22530"
                     /old_locus_tag="R1158_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9070..9384
                     /locus_tag="R1158_RS22535"
                     /old_locus_tag="R1158_22535"
     CDS             9070..9384
                     /locus_tag="R1158_RS22535"
                     /old_locus_tag="R1158_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            9548..9976
                     /locus_tag="R1158_RS22540"
                     /old_locus_tag="R1158_22540"
     CDS             9548..9976
                     /locus_tag="R1158_RS22540"
                     /old_locus_tag="R1158_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     BGC             365..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4183..4761
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(689..1416)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gctgttgctg gaaaagctga acgagctgga catggatacc ccggccgaca tgtgcgaacg
       61 gcttcacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaagaata
      121 acagcaccgg ccatgccggt gctggcgtta ttttttgccg aacggccacc accggcgggc
      181 gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg
      241 catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac
      301 ctcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc
      361 gttctcgatg gcgaaccgct ccacctttcg atagaggtaa tcacacagct tcattcctgt
      421 gggtttgtcc cgttcccatt gcggattttc accggtgaaa aagtacgtgg ctttttcgtc
      481 gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg
      541 gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca tgtcttccag
      601 atgctcgctg gttggcataa tgcagcgctc gccgtctttg tcttccacgt cctcaataaa
      661 taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc acgcgaacag
      721 cctctcccgg tccagctcca ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc
      781 atccaccgcc agaatcgccc gctgtacccg gacaagcgtc ggccggtcag ggcagatgta
      841 ccagatttca tcgatgccgt tcgcccggcg gttgaacaga tacccggaca ccacttcctg
      901 ataccgcctg cggctcttga cggtgcgctc tacctcaaat gccaccgttc tgccgtccag
      961 ctccgccagc gcgtccggca ctttcatccc tttcagggcc atacggtgca ggtaccgcca
     1021 gccggtggcc ccccggccct ccatggagag gcgcagggac tgcacggcca gccggtgcgg
     1081 cagctgcgcc gccgacaccc ttgacggctg aaacggaata acgtccgtca gcggctcgtc
     1141 ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc
     1201 gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc accagactgt ccaggaagcg
     1261 gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca accagctccg ccgtggaaca
     1321 cagctcttcc cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg
     1381 cttcgcgtgg ctgacgttgt actgctccag tgtcatcagt aaagccccgc aaaatcatca
     1441 tccgcttttg gtccgccgcc gtcggccgga ggcaggtact ccgccagcgg gtccgttttc
     1501 gccagcaccg ggaaggtttt cagcgaaatt tcccggcgct caacccgcag cgggctgctg
     1561 aacgccagcc gcgccacgcc cagcccggtc atcaccgcga acccgtcagg caggtgttgc
     1621 agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc gccgctgatg
     1681 tgctccacgt tccccgcctc ccggctggcc cggcggcgct ccacgtccac ccggatctcc
     1741 ccggtctggc ccgccgccca ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg
     1801 atcggcgtgt tatccagtac cgtggttttc acaaaatcag cgggcagatc ctgcccgcac
     1861 tgaccgaagt cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc
     1921 gtccccagcg cgttcagcac gtacttcgac agcagatatt taatctcatc gagcatgatg
     1981 ctcgcgtgcg gccacctccg gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca
     2041 aacagcatct tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg
     2101 cagccgccgc cgttgattat ccccgccaca tcgataccgc tgtccgtctg caggacgctc
     2161 aggtcggcca cgttctccag cttggtgatc agccccttca cgtccttttt cagggcctcc
     2221 ggcaacgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc
     2281 gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc
     2341 tcactcaggt taaacccggc aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg
     2401 aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccgggcag
     2461 tgcgcccgga agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta caccacagcg
     2521 tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc
     2581 ccgggcaggc cgaggatctg aatattggtc ttgcgccagg tatcacgagg gaccagcacc
     2641 ggctggccct gctcatcctt acccagaaac accgcctgct gtttccgggc ctgctccgtg
     2701 gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg gcggacgtct
     2761 gtcatttctc cggtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc
     2821 gcctcgtcag cggccgcaaa atgaccatca agcagcagga acgccagcag ccgcgccaga
     2881 aggacgccgg cgacaagccc tccggcacac caggctatcc cgaacaggtt agccggctgc
     2941 agcaggtaat aatctgcgtt cgcatagagg tccgggcgca ggacggtttc tccccagctc
     3001 tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggcagc gccggtcagc
     3061 cagtacgcgt agttccagac atgtcgccgt gccctgacca tccgcccggg tgaatccagc
     3121 gaggccagca ccgaaaatgg cacagtccgg ttattatcgc gcttatagcg atcggcatac
     3181 tcccgccgcg ccttatggct cagccccaca aaacgcattc ggccggccca gagttcagcc
     3241 agaccgaaaa acaggccgat aaccagaaaa aaaccggcca gactgtaata ctcccggtac
     3301 agccagctga cccacgggta aatctgccgg gccagctccg ggatatgcca ctgccaggca
     3361 agcgaatccg tattaaacat gcaatctctc tccttcagac gacgggttta ccggcgtgat
     3421 gcgcagcccc cgctgcacaa gccgcggaca tgccccaagg catggcggcg gcggtcacgt
     3481 ccgtggtgat tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa
     3541 aaggcgcagc cccggaggaa caacgagcac gccccagcgt gtgagaggaa aaggcggcac
     3601 cccagtgcgg tcttttccgg tgttccggcc cccgtcagag ttcgccatag cgagcggctg
     3661 accacccgca aaatcaggag cagctttgct gcttcgtcag gtgcgtccag caccgtggat
     3721 ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtggccggc
     3781 gtccagccgg acagagccag aaagagggga aaagaccgtg cgtcggtggc gtccagccag
     3841 cgtaagcaag gtcttttccc ctgaagtcag ccacgcccca gcggggatga cttgcatttc
     3901 tccgcaagga gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga
     3961 catggttcac ctatcctgat aattgatcgt cagggaatag aaagacttaa tcaggggaca
     4021 atatcccaca tcagcgcgtc cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct
     4081 cgctcactcg gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca
     4141 aagttaccca cagattccgt ggataagcag gggactaaca tgtgaggcaa aacggcaggg
     4201 ccgcgccggt ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac
     4261 gctttttcgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg
     4321 aaacccgaca ggacttaaag atccccaccg tttccggtag gtcgctccct cttgcgctct
     4381 cctgttccga ccctgccgtt taccggatac ctgtccgccg ttctcccttg cgggaagtgt
     4441 ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag
     4501 ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt
     4561 tcacttgagt ccaacccggg aaagcacgca aaacgccact ggcagcagcc attggtaact
     4621 gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa
     4681 gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg
     4741 gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt
     4801 cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4861 tttctactga accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccgcc
     4921 ctgaacatcc cggatataat ttgcctgaca tgcggccaga agaaagccag caacaatcaa
     4981 tataaaaaga aaaatagtct ttaccttttt catatacttt ccctgtattt aaagactccg
     5041 gcattcatcc ctgaacaccg gaagtcagta tttcaaatca tccttgataa ttttttgtta
     5101 ccagttaccg tcccgataat caaaagctat ataacaatta tccacatcag gttttatttg
     5161 atttttaaaa aatggctgta gtaaaggaac ccattctttc ttcaaatcaa actcaccatt
     5221 attaatatta tcctttaaag gcattcccaa gctttcaatg acagaaccat cgtcaccaaa
     5281 gtctttagaa tactcttccc ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg
     5341 taatttaagc cccatattta cctctcaaag atatttttta atgttccgtt ttggatctgg
     5401 ccctttaacc tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc catcactggc
     5461 acgataccct tcaagctcac cgtgctggga atcccattca taaattttac gttttttatc
     5521 gccataccat cgggcccgct taccaccacc accttgttta ggtgttttag gatcacctcg
     5581 ctttaactct ccaagtccct ttatgtcttc tgttttaggt actggatgat aatcatggcc
     5641 gtagtcttta gtccccttgc gaggcttgtt cttttcctca tttaacttat tttctgcatc
     5701 cttcgctttt ttctcctttt gtttccggct ctccatcgcc ccggtaagtg cagccttatc
     5761 tctggataag gattcattca ttgcgtttaa agatgcctga gccgctttta atttctcatt
     5821 ctgattctga atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc
     5881 atgaggcggg ttataagtca caaaatcatc tttatttgcc tctgcctctt ttaccttttt
     5941 atcggcttcc tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg
     6001 gctgtttaag gtcgcaatat ttttatcttc ggcatccagc tcagctttcg ctttatcata
     6061 gtctcttttc agcccctctt ccggatgagc ggcgtcccat gcctgctggc gacgcttttc
     6121 ttcctcctga cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg acacataaac
     6181 cggcttctgt ccggtctctt tcgggaaacg aataacggcc tcacgggagt taccaccggc
     6241 tgtaaaacca gccggacgtg aatcaccttt ttcagcaaca atgccttttg gcggggcttt
     6301 cgctgtcgga acacctttcg gtacgctcac ctgcagagac gggagacccg gaatcgacac
     6361 actgaatacc cccggacgtt ttgtcggttt cgcatccacc acagggacac tcattggccg
     6421 acctgcgaca accgcaatat gctgccgctc atccttcacc acatccacaa cccgttgtct
     6481 gacacgaacc gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc
     6541 ggccggtaat gacgtcataa tttttgacat cattttcgga tcatcttttg caatctcaga
     6601 aggcagcaca ccgtagatcg caatccccca cagaccaaac ttaaacggcc ccttcagggc
     6661 agccagcaca tcagcaacgg cggccgacaa cgcatcgccg gaaactgata aagccagtcc
     6721 ttcagccccc ggagtagcca gggcaggcag accgaaggcc atcgcagtgg ccgaagactg
     6781 tccgccacca gagctaccgc tatgattgcc gctgttacca ccattaccac cgggtttact
     6841 cccgccgtta ccaaactccc ccggattata gttatgaata tctccattcg gcgtatgcgt
     6901 cgtaccccag cccggcccgc tgtttggatc cgtaccacca cctgatgaag ggccaccttt
     6961 accagacgta ccgttcacat tcccactggc ctgaccacca ttatgtccca gacctgaatt
     7021 acccggacct cgaccgtctc caccactcat accgttttcc tctttgacat ttaaaacaat
     7081 aaattaaagc caacatactg tacatataca cagtataatt attaccttac tcctcccgtt
     7141 ttcaggtgtc aatatatgag attagttctc atcttcaggc acaaaaaagc gcccggagcc
     7201 acctgtaagc agatcgcgtc ttttcccgaa aatttctcat ggcaacaaat tttattattt
     7261 aagacattca tcacacttac atttcagatg gtatagacac atctgaaagc taactggcat
     7321 gaggagagga cctgcggtta aactgttcgg gaaacctgag acgcccggag gccactatga
     7381 cgcacctgaa actgactccc gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg
     7441 cactggccat gggcctgaaa gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc
     7501 gggaaagggt gcttcgtgcc attcaggacg aacgtgaagc caggcgggcg caggaaatgc
     7561 gtcgggagaa atacgaggct acggaaacca ccttcacctg gcagccacag cgccgccggt
     7621 aaggggtatt taccataacg tcgcattgcc cgcaccgctc agacggtctg agggcgtttc
     7681 cggcggctac gggataaaac aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg
     7741 gtaaattgta acccgctgca tgcctatgca cgcaactgca tggcgggccg tttggtgtaa
     7801 aaacgatacg ataagccgtg tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata
     7861 catgtaacat tttttactga caggtcatca acccgatgcg tttaataccc tttatcctgc
     7921 tggcattgtg cagcccctta ctcctgacgg cctgtaagca ttccaccacc cagccgccga
     7981 aaatttatac tctggataag tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg
     8041 ctgttgaaat gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga
     8101 tatccataca acgtgatggc actctgaaca gcgccattgc tgaaggagga gatccgaaac
     8161 tctgtaaagc agcaatatca gccgttaccc acgccaaaat tccgccggca ccggatgaaa
     8221 atacctggca aatattcaaa aacgcccctc tggatttcag accatgaaag cttttactct
     8281 gacagtgctg gcggccatgt tcctcactcc ggtacatgcg ataccactaa aacccgtaac
     8341 cacactgcag ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac
     8401 cgccacgttt gatattctgg acgatggacg agtggacaac gtgtcgctta ccggctgtca
     8461 cctttttttt cgggaaacgc tgacggcaat gaggcactgg ctatatcccg cagggcaccc
     8521 ggcaatgcga caggttataa attttgaatt cagtctgaaa aatgacggct tacaggatcc
     8581 gcgaccagtc accacccaaa agctcaatga tgatggttgc cgccagtaac aggtccttta
     8641 acccgcaggc tgcaatagtc ctggatgatg agtgagcctg tagcagccct gattagtgga
     8701 agcgaagctg aaaaatgggc agcaaaagcc gtatttctgg caaatagcgc cgccacggat
     8761 taaagtagtc atgtgtaata catatgacta caaaggggct tttatgtcat cagcaacatc
     8821 catcaggctc gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg accggcaccg
     8881 ctcagcgcac gcgctaatgc tggaggcaat caccgaatac atcgaccggg aagagaagcg
     8941 cagccagtac ctgcgggacg gacaggccgc atggcagcac tatcaggaaa cgggtctgca
     9001 ccttactgcg gaagaagcgg aagcctggat aagtacctgg ggaactgaga acgagcagga
     9061 tgctccgcca tgccacaggt aacgatttcc gcgctggcac agcgcgattt acaacgcctt
     9121 caggactttc tcaaaaccaa aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc
     9181 cgggctatcc agcaactgaa aacattgcca gacattggcc gcccggtgcc gtttctgccg
     9241 ctggaatata aggaactggt gatcgggttt ggcgacagtg gctatgtgat gctctaccgc
     9301 cacgaccggg aaatggacca gattgtgatc gtcaccgtca ggcatcagaa agaatccggg
     9361 tatccggggg cagacagcct ctaataaccg atgacggggg cgcaattcat tgcgtggccg
     9421 tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa
     9481 aattcagcga tacatttacc gtgagaaatt acacaccttt cacacccgat aaaatcaggg
     9541 gtatacagtg gcgaaaacat tgctcaacct ttcacaggca gcacaagccg cgggcattac
     9601 cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa
     9661 aaacaaccct gtcgtggatg tgtcagaact gatccgtgta tacggaaatg tgaatatccc
     9721 tgagaaacaa attcccggca tttcacaccg tgaaaataca caaaaaaatt tcccacagga
     9781 acagcttctg gcaatgcaaa aggaactggc cgatctccgg caggctgtca ccctgatgct
     9841 ggaagataaa acatcccggg aagaagaacg ccgccagcac gatgatgagc gccggaaact
     9901 ccaggccgaa gtggacagat tgacaacaga actgacacag aagaaaaaac gcttctggtc
     9961 cggctggttt agctgagaac ttaccgaagg gaatggagaa catgacgaag caggaaaaaa
    10021 ctgccctcaa tatggctaaa ttcattaagg atcagtcgct
//
