LOCUS       NZ_CP136612             2014 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter portucalensis strain L2724hy plasmid p11-L2724hy,
            complete sequence.
ACCESSION   NZ_CP136612
VERSION     NZ_CP136612.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37738783
            Assembly: GCF_032917905.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Guo,K.
  TITLE     SFO-1, KPC-2, NDM-1 were first reported in Portuguese citrobacter
            collected clinically
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Guo,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2023) Basic Medicine, Zhejiang Chinese Medical
            University, Puyan Street, Hangzhou 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicylers v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:18:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,316
            CDSs (with protein)               :: 5,316
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 90 of 124
            Pseudo Genes (internal stop)      :: 25 of 124
            Pseudo Genes (multiple problems)  :: 24 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136612.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="L2724hy"
                     /host="Homo sapiens"
                     /db_xref="taxon:1639133"
                     /plasmid="p11-L2724hy"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-09-11"
     gene            complement(join(1983..2014,1..400))
                     /locus_tag="RY846_RS27800"
                     /old_locus_tag="RY846_27800"
     CDS             complement(join(1983..2014,1..400))
                     /locus_tag="RY846_RS27800"
                     /old_locus_tag="RY846_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_126486825.1"
                     /translation="MRKVTQVDLETGEDLGGFVAVIRPKQKSSFERHFTMNQAALKIIA
                     TELNHEQTKVLMMLLADLDYENYIQVAQIDIAESLGMKKPNVSKAVKNLIEFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSIIQGGKV"
                     /name="helix-turn-helix domain-containing protein"
     gene            1516..1917
                     /locus_tag="RY846_RS27805"
                     /old_locus_tag="RY846_27805"
     CDS             1516..1917
                     /locus_tag="RY846_RS27805"
                     /old_locus_tag="RY846_27805"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_058671968.1"
                     /translation="MNNREFKEIRLSAGLTQAEFASRLGLARETVCRIERCAYPVSRGV
                     SSLAKSLLNPDPATVLQLKSDNLSHDGSDALIAAKCDEISLLAKLDKMIASFRYSRGLY
                     GRNKVATKRVLEFLSELEREILPRLHPYK"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 cgtttttgag tgcttttttg tggttgctta ccgtgccttt ccagccgaac tgagggttca
       61 ggcggtaggt tttgcttcgg cctatctttg gcccctcaag gattattccg aactcaatca
      121 ggtttttaac agctttgcta acatttggtt ttttcattcc caatgattct gcaatgtcga
      181 tttgtgccac ctgaatgtaa ttttcgtagt ccaggtctgc gagaagcatc atgagtactt
      241 ttgtctgctc atggttcagt tctgtagcga tgattttgag tgctgcctga ttcatagtga
      301 aatgcctctc gaacgatgat ttttgcttgg gacggatcac ggcgacaaac ccgcccaaat
      361 cctctccggt ttcgaggtca acctgtgtga cttttcgcat gaaaattcct taaatagtta
      421 tgcctgaaca taactaagtt atgcctgaac ataactatct gtcaaatgat tccgcttgtg
      481 gcgcggcttt caggaggttc ccttctgtat cttgtaactc tgtgctcgat gccaaccacg
      541 aaaacatcac ttcgggaacc tgctgtaggc atcgggaaac gtgcacactt tgtcgggttt
      601 caccctatcc tgcatcacgt ttttctgtag gtactgtgcg tacggttttc tatgcccgtt
      661 caggtgatat tccgcttcct cgacgtgaca gactcgcgtt gctcgctaat cacgtcttgc
      721 ggcgagcggt gcggactctc tctcgtggtt tctggctgtc tgtgccgtat gggtcagaat
      781 ggcacgagct ggcaggcggg cgcgggaagg gagcgcaccg agttcccacg aggagtgcac
      841 ccttcccgtg gcggcttgcc cgtatagcgg aattatgatc tgatggcgca acgggcagag
      901 atttgcgctc tccgccattt gctatcacga aaacctgtta cggaggtctc catgactgac
      961 acgaatccta ccatgcttgc catgattaca cgtcaccaga acgccgtaga ggcatttacg
     1021 gcgtttgcct cactccatcc ccttgatgat gaccttcatc ccctcataga gcttctgagc
     1081 gcgaatttgt aggcgtcttt tctggatatt catccgcatc tctgtggtct gaactcgtca
     1141 aacgctaaat cgggggttcc ctttcagggt tccgatttag tgattgacga cacgccggag
     1201 aaataacact ggtaacgggt gatggttcac gcagtgaggc catcgccctg atgagccgtt
     1261 tttggatgtt gccgcgaatg gcggtactcc aaaaacacat cttgtccggt ttcgacatca
     1321 aaggcaggac aggatgtgca ttgtgtacac cgccgcaccg atacccccgc cctgtaacac
     1381 gggggtagag tcaacggaaa cacggagttt tccagttaaa acctaacttc tggtagcgtg
     1441 cgggtagact catcggtttg ttgaggtgat gtaatatcac atccttaaaa tcacgagagg
     1501 atcgattcta accctatgaa taacagagaa ttcaaagaaa tcaggctttc agcggggctt
     1561 acacaagctg aatttgcgtc ccgtctggga cttgcccgtg agacggtttg ccggattgaa
     1621 cggtgtgcct atccagtttc tcgcggcgtt tcctcactgg cgaaaagcct gctcaaccct
     1681 gaccctgcaa cggtcttgca gttaaaaagt gataatttat cacatgacgg tagcgacgct
     1741 ctcattgcgg ctaaatgtga tgaaatatca cttcttgcaa agctggataa gatgattgcc
     1801 agcttccgct attcacgggg gctttatggc agaaacaaag tggctacaaa gcgggttctt
     1861 gagtttcttt ctgagcttga gcgggagata ttaccccgcc tgcatccgta caagtgatgt
     1921 aacatcacgt ggggttaagt gcagtacagt gatactgcac ttttgtatca agatggtacg
     1981 gctcacacct tgccaccctg aatgatactg aggc
//
