LOCUS       NZ_CP136611             2153 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter portucalensis strain L2724hy plasmid p10-L2724hy,
            complete sequence.
ACCESSION   NZ_CP136611
VERSION     NZ_CP136611.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37738783
            Assembly: GCF_032917905.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Guo,K.
  TITLE     SFO-1, KPC-2, NDM-1 were first reported in Portuguese citrobacter
            collected clinically
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Guo,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2023) Basic Medicine, Zhejiang Chinese Medical
            University, Puyan Street, Hangzhou 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicylers v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:18:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,316
            CDSs (with protein)               :: 5,316
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 90 of 124
            Pseudo Genes (internal stop)      :: 25 of 124
            Pseudo Genes (multiple problems)  :: 24 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136611.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="L2724hy"
                     /host="Homo sapiens"
                     /db_xref="taxon:1639133"
                     /plasmid="p10-L2724hy"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-09-11"
     gene            436..549
                     /locus_tag="RY846_RS27780"
                     /old_locus_tag="RY846_27780"
     CDS             436..549
                     /locus_tag="RY846_RS27780"
                     /old_locus_tag="RY846_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            857..1159
                     /locus_tag="RY846_RS27785"
                     /old_locus_tag="RY846_27785"
     CDS             857..1159
                     /locus_tag="RY846_RS27785"
                     /old_locus_tag="RY846_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_023207139.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGVHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            1414..1602
                     /locus_tag="RY846_RS27790"
                     /old_locus_tag="RY846_27790"
     CDS             1414..1602
                     /locus_tag="RY846_RS27790"
                     /old_locus_tag="RY846_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1650..1850)
                     /locus_tag="RY846_RS27795"
                     /old_locus_tag="RY846_27795"
     CDS             complement(1650..1850)
                     /locus_tag="RY846_RS27795"
                     /old_locus_tag="RY846_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045351243.1"
                     /translation="MSLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRHIVSDTNQAR
                     RSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     Typing          complement(2049..2133)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="100.0"
                     /evalue="3.06e-32"
     Typing          2..545
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.139"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gtggcaggag gaaaatgcag cagcagagcg taagcggaat atatgagaca ggagatattc
       61 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtgcgggc
      121 ttacgaatgg ggcggaaatt tcctgggaga tgccaggaag aaacttaaca gggaaacgat
      181 aaggccgcgg agaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
      241 tgacgctcaa atcagtggtg gcgaaacccg acaggactta aagataccag gcgtttcccc
      301 ctggtggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
      361 tgttatggcc gcgtttatct cattccacgc ctgacactcg gttccgggaa ggcagttcgc
      421 tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc cttttccggt
      481 aactatcaac ttgagtccaa cccggaaaga cacgacaaag cgccactggc agcagccact
      541 ggtaactgat ttagaggagt tagtcttgaa gttatgcgcc tgttaaggct aaactgaaag
      601 aacagatttg gttcctgcgc tcctcataag ccagttacca cggttcaaag agttgctgct
      661 ctgagaacct tcgaaaaacc gccttgctgg gcggtttttt cgtttacaga gcaagagatt
      721 acgacgctac agaaaggatc tcaagaagat ccttcaccat aatggaaatc tatcccaccc
      781 gagcatatag gatgcatatc gttataaaac atcggatatt atgcatccca actaaaaata
      841 agctcgagga taaaaaatga gttcaatggt ttactgccga ggatgcggca aggaaataca
      901 cgaaacagca aagtcatgcc cacactgcgg agccacaaac gcctcttcag gttcaggtga
      961 aaaaagtaga atcgcagccg cgctattagc attcttcctt ggtggatttg gcgtacataa
     1021 attttactta ggtaaaatag gacagggatt cctgtatcta attttttgct ggacattcat
     1081 acccgcgatc atcgcgttta ttgagtttat tatttatcta tgcgattccg atgaaaaatt
     1141 cgcaagaaaa tatggctaac tcatagcaaa aaagccctgc ggggcttttt ttgtaccagt
     1201 ctgtgaggca gatttaccat aacatccgtt gcgtgcaccg cctgagaggc tctcagggcg
     1261 tttctacgac ctcacctgca aaagaacaca tcttaccccc tgaaaaccgc tcagaaacgc
     1321 gctgatgatt tctgcgtacc tgcatggcta tgcactcaag tgaatgaagt aagatttcag
     1381 tctgcgatca ccataaaacc gggaaaaagc cgtatgacaa aacaggaatc agccgcactg
     1441 aacatggcaa aatttatccg ggcgcagtcg ctgctgctgc tcgaaaaact cgacgtgctc
     1501 gatcttgatg acgaggcagc cgactgtgag cgaatgcacg aacaggcaga agcgctttat
     1561 cagaagctga gcgcacgctt cggtatacag gacggcgaat aaagagacga aaagaacgct
     1621 cacttagacg aaaagcgtat ccagggcaac tatgtatatc caccgtgtac atagtcatgc
     1681 ctgacgcttt cgagcaggtc gagcatgtag gaacgacgag cttgattggt atcggacaca
     1741 atatggcgat acgcctcaaa cataacagca cccgcatcac cgcccgaacg gcgaagcata
     1801 gcggaaatca tccgcacaaa aagcagacgt tcatcggggc aaaggctcat aagttctcct
     1861 ggtgaaaaac caaacatgaa gcacccatct attaacgtca gctgtgaacg cttcattaga
     1921 agaaaatagg taaacccggg aaaaagatcc aaacgttaac atcacaggta aacagaaacg
     1981 tcgggtcgat cgggaaattc tttcccggac ggcgcggggt tgggcaagcc gcaggcgcgt
     2041 cagtgctttt agcgggtgtc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt
     2101 gtatactggc ttactatgta ggcacgagtg cgggaatcag tgaagtgctt cat
//
