LOCUS       NZ_CP136610             2182 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter portucalensis strain L2724hy plasmid p9-L2724hy,
            complete sequence.
ACCESSION   NZ_CP136610
VERSION     NZ_CP136610.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37738783
            Assembly: GCF_032917905.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Guo,K.
  TITLE     SFO-1, KPC-2, NDM-1 were first reported in Portuguese citrobacter
            collected clinically
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Guo,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2023) Basic Medicine, Zhejiang Chinese Medical
            University, Puyan Street, Hangzhou 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicylers v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:18:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,316
            CDSs (with protein)               :: 5,316
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 90 of 124
            Pseudo Genes (internal stop)      :: 25 of 124
            Pseudo Genes (multiple problems)  :: 24 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136610.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="L2724hy"
                     /host="Homo sapiens"
                     /db_xref="taxon:1639133"
                     /plasmid="p9-L2724hy"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-09-11"
     gene            complement(204..572)
                     /locus_tag="RY846_RS27760"
                     /old_locus_tag="RY846_27760"
     CDS             complement(204..572)
                     /locus_tag="RY846_RS27760"
                     /old_locus_tag="RY846_27760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048232015.1"
                     /translation="MALLISLVVVAGLYYQFWYKPRRTPILRVWGIAPNGQRFRLLLTE
                     HGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYLEDAHKNELLIELNESDLYPGGAIEFQ
                     QKGGRKINCEILKVRWSR"
                     /name="hypothetical protein"
     gene            complement(766..942)
                     /locus_tag="RY846_RS27765"
                     /old_locus_tag="RY846_27765"
     CDS             complement(766..942)
                     /locus_tag="RY846_RS27765"
                     /old_locus_tag="RY846_27765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164714441.1"
                     /translation="MPETALMRQDCAEGACGGAGKGCYGKYSYFVRTKEIGEIHSAAPI
                     YEEILKYEKLDSS"
                     /name="hypothetical protein"
     gene            complement(1067..1303)
                     /locus_tag="RY846_RS27770"
                     /old_locus_tag="RY846_27770"
     CDS             complement(1067..1303)
                     /locus_tag="RY846_RS27770"
                     /old_locus_tag="RY846_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1991..2149
                     /locus_tag="RY846_RS27775"
                     /old_locus_tag="RY846_27775"
     CDS             1991..2149
                     /locus_tag="RY846_RS27775"
                     /old_locus_tag="RY846_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     Typing          complement(1469..1642)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          1566..2100
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.182"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 tgcggtctcg tatacccagt tacctggtta agcagttccc caactgactt aaccttcgat
       61 caaaccacct ccccaggtgg ttttttcgtt ttcagagcaa gagattacga ctgtaacgac
      121 aggatcacaa gaagatcatt tactccgaga gagtaactac caccatgttt gcctctcaac
      181 acacttagga aacattactt gagttaacga ctccacctta cttttagtat ctcgcaatta
      241 atcttccgac cgcctttttg ttgaaattct attgcgccac cagggtataa atcactttca
      301 tttaactcaa ttaaaagctc gttcttgtga gcatcctcta aataaaggta ataacaccca
      361 ccataaaggt tctgcgtatc tggcacggca acaaaagatt tctttccgaa cagtaaaact
      421 gcctcttttt ctccatgctc tgtcagcaat aatcgaaatc tttgtccatt tggcgcaatc
      481 ccccacacac gtaagatcgg cgttctacgg ggcttatacc aaaattgata gtaaagccct
      541 gccactacaa ccagtgaaat taataaggcc attttaatcc tcaaaaaata cagtatcccg
      601 gaattttttt attcatttgt actgcttgga atagtgatcc aaaagataac acctgaaact
      661 aaaagaatcg acccagctac atacgttaca taaaccccaa cataagagac cgtcagccat
      721 ataagtaagt atatagctag ggtaaatcca aaaataattt tggccttatg atgaatccag
      781 tttttcatat tttaaaatct cttcataaat tggcgcagct gagtggatct caccaatctc
      841 ttttgtacgc acaaagtagc tatatttacc ataacatccc ttgcccgcac ccccgcaggc
      901 gccctcagcg caatcctgcc gcatcagggc cgtttccggc acctttcctc accagtatcc
      961 cggaaaaacg cccctgatga tttctgcgcg actgcatggc tatgcacgca ggtgaatggc
     1021 aggtgatttc gcagcgtcag caccactaaa ccgccaaaag ccaaaatcag cggcgcggcg
     1081 cgcgcggacg tgaccaggca aaatcggact gctgccgctc atcctcacgc aatgcacgct
     1141 ggcggtcggc gtaacgctgc aactcaattt gcgcacgcgc tctgttcagg acgatgtgcc
     1201 ggttaggctg atttagcttt ttacctgacg ctctttcagc tgcggcaaca gcatcatcaa
     1261 gaaatttttt ctctgcaggc gtcaagtttg tcagtctggc cataacgttc tccagtcaga
     1321 ataaatcagc acagtctgcc agacacagta gaaacgatga agcgccagcg acggtgaaag
     1381 gcagtcagaa acggtgaacg ggagcggatt ttaatccggt gccgtgaggc gttggacaag
     1441 ccgcaggcgc gtcattagct tttagcgggt ttcggggcgc agccctgaac cagtcacgta
     1501 gcgctagcgg agtgtatact ggcttactat gttggcacgg atgcggaaat cagtgaagtg
     1561 cttcatgtgg caggagaaaa aaggcggcaa cggcgcgtca gcagaatatg cgatacagga
     1621 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcggctg cggcgagcgg
     1681 taccggctta cgggcgtgac ggaaattccc tggaagatgc ttagcagaga ggcgataagg
     1741 ccgcggcgaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1801 gctcaaatca gtggtggcga aacccgacag gacttaaaga taccaggtgt ttccccctgg
     1861 tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     1921 atggccgcgt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     1981 gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta
     2041 tcgtcttgag tccaacccgg aaagacacga aaaatcgcta ctggcagcag ccattagtaa
     2101 ctggaggtat acgagagaca aattgagagg tcttgaagta tgggcctgag agaggctaca
     2161 ctgaaagaac agttttggtt tc
//
