LOCUS       NZ_CP136609             5040 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter portucalensis strain L2724hy plasmid p8-L2724hy,
            complete sequence.
ACCESSION   NZ_CP136609
VERSION     NZ_CP136609.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37738783
            Assembly: GCF_032917905.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Guo,K.
  TITLE     SFO-1, KPC-2, NDM-1 were first reported in Portuguese citrobacter
            collected clinically
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Guo,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2023) Basic Medicine, Zhejiang Chinese Medical
            University, Puyan Street, Hangzhou 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicylers v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:18:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,316
            CDSs (with protein)               :: 5,316
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 90 of 124
            Pseudo Genes (internal stop)      :: 25 of 124
            Pseudo Genes (multiple problems)  :: 24 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136609.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="L2724hy"
                     /host="Homo sapiens"
                     /db_xref="taxon:1639133"
                     /plasmid="p8-L2724hy"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-09-11"
     gene            11..502
                     /locus_tag="RY846_RS27730"
                     /old_locus_tag="RY846_27730"
     CDS             11..502
                     /locus_tag="RY846_RS27730"
                     /old_locus_tag="RY846_27730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_326926120.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWLGQQIL
                     DNQTAIRQQEDTLTKMTARTWGVRYQESSDGRKFLILPPGMQAEAIPYEGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            509..724
                     /locus_tag="RY846_RS27735"
                     /old_locus_tag="RY846_27735"
     CDS             509..724
                     /locus_tag="RY846_RS27735"
                     /old_locus_tag="RY846_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_326926121.1"
                     /translation="MTELEKQLLSALEQLQQDYWQRLDEWESAFEEWRRMSGLMQRENA
                     MLSERVSDLSEQVQRLSDQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1190..1429)
                     /locus_tag="RY846_RS27740"
                     /old_locus_tag="RY846_27740"
     CDS             complement(1190..1429)
                     /locus_tag="RY846_RS27740"
                     /old_locus_tag="RY846_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="WP_326926122.1"
                     /translation="MNISIKAIRFDKYNMWVALNDARTIGIPLRSFPKLMQADHEQLSK
                     FEISYIGIHWDVLTEDISMNELLFINSNTKNISR"
                     /name="DUF2442 domain-containing protein"
     gene            complement(1681..1872)
                     /locus_tag="RY846_RS27745"
                     /old_locus_tag="RY846_27745"
     CDS             complement(1681..1872)
                     /locus_tag="RY846_RS27745"
                     /old_locus_tag="RY846_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_326926123.1"
                     /translation="MNRQESAALNMAKFIRAQTLLLLEKIDLLDLDDEATVCEKLHEQA
                     EMLYQKLSSRLEPDDNER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2064..2330)
                     /locus_tag="RY846_RS27750"
                     /old_locus_tag="RY846_27750"
     CDS             complement(2064..2330)
                     /locus_tag="RY846_RS27750"
                     /old_locus_tag="RY846_27750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_326926124.1"
                     /translation="MPLEQAAFLFSGVGGGMLLTEMEISNTRNITLIARRLAWINAKRT
                     HIDIPSFAELLFSGQKALFFEQYNYFHSRRYTTAGTSKPPDLP"
                     /name="hypothetical protein"
     gene            4050..4373
                     /locus_tag="RY846_RS27755"
                     /old_locus_tag="RY846_27755"
     CDS             4050..4373
                     /locus_tag="RY846_RS27755"
                     /old_locus_tag="RY846_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_326926125.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQTARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3859..3943
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="100.0"
                     /evalue="3.0800000000000004e-27"
     Typing          4051..4367
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.3999999999999998e-60"
     Typing          4364..5040
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.903"
                     /coverage="99.0"
                     /evalue="2.36e-140"
ORIGIN
        1 ggagccatct atgagcagtc ttttaacgct ggcgaaggac ttagagcagc aatcgaaagc
       61 gcagcaacag agtaccggcg agatgctgaa agccgcattc agcgagcacg agcagtctgt
      121 cagagcggaa ctgaacgcaa gcgcgaggag aatcagcgac gccatcagcg cccacgagca
      181 gagtatgagc gaggctatgg agaaaaaccg gcggagcgtg ctgcggactg ccgggcggac
      241 atggctcacc atcctgatgg tgtcagcgct gctgatcggc acgagcggga gcattctgtg
      301 gtggctgggc cagcagatac tcgacaatca aacggccatt cgccagcagg aggacactct
      361 gacgaaaatg accgccagga catggggcgt gcgctatcag gagagcagcg acggccggaa
      421 gttccttatc ctgccgccgg ggatgcaagc cgaagccatc ccctacgagg ggacaacctg
      481 gatccgactg aaacaggagt agagaaacat gacagaactg gaaaagcagt tgctgagcgc
      541 attagagcag ctacagcagg attactggca aaggctggac gaatgggaga gcgccttcga
      601 ggaatggcgg aggatgtctg ggcttatgca acgggagaac gcgatgctga gcgagcgcgt
      661 gtcggacttg agcgagcagg tgcagcgttt gagcgatcag ctgcgccgct tgtcgaaagg
      721 ctgaatgcta ttgagacccg agagatgcag taccgaagaa tccaggcaat ggaacgtcaa
      781 tataatcaga agtacaaagg ttttgagcgt tgatgtatat tcacccggta taaaaacagg
      841 ttgttagtcc atgtctgtag ggagtaatcc acttattgaa tctttttaaa gatgcgctgg
      901 attaatttcc tctctttttt agttaataac tctgaattgt ttatttggtt tttttgtatc
      961 ttgtgtatgt ttgctttctc tataaaaaag tcaatctcaa atgattttaa tttgcatctg
     1021 tcatcaacag ataaaagtaa aatctctatt cgcttgaata gactggtatc gtcattccgc
     1081 ataacattac tgtccatttt tttatttatc aatgacttat tttttttaat gggttgggtg
     1141 tgtttttctt ttgtctgaaa aatgttttaa gtgttaacca tctacggaat tatctactga
     1201 tgttttttgt attgctattg atgaatagta gttcgttcat ggaaatatct tcggtcaaca
     1261 catcccagtg gatgccgatg taacttatct cgaatttact gagttgttca tgatcggctt
     1321 gcatgagttt aggaaacgat ctcaacggga taccgatagt gcgggcatcg ttcagcgcta
     1381 cccacatgtt gtatttatca aaccgtatcg ccttaattga tatgttcatt cagacctcat
     1441 cttgattttg tctgcgagaa gtaataatcc tagcccaata tgtaaaattt tttaatgtac
     1501 aggattgacg gacggggcag gaatcagcga taacgttact gtgacttctt gacgcctcgt
     1561 gtcattattt agtcaataaa aacaagaaga tgaattctat cgattcccga ctcccgtgtc
     1621 gggttttttg cctggttgcg ttccaatacc tcagatccac gcaataaatt acgtgcccgt
     1681 tcaccgttca ttgtcatcag gctccaggcg tgaactcagt ttctggtaaa gcatctcggc
     1741 ctgttcgtgc agtttctcgc ataccgtcgc ctcgtcatca agatcgagca gatcgatttt
     1801 ttccagcagg aggagggtct gggcgcggat gaatttagcc atgttgagtg cggccgattc
     1861 ctggcggttc atgtatgttt tttccggtct ggttattatt tcttggtgaa cagagcatga
     1921 tgtcattctg gtgcatagtc atgcagattg ttaaaattta tctgtcgcga tcggggcgat
     1981 ttctcgggtg gtttgttacc tgttttacct gacagtcgtc ccgttcgtgg cgaacctgtc
     2041 cggggcggtg cgggcaacgg atgttatggt aaatccggtg gctttgacgt gcctgccgtt
     2101 gtgtaccgtc gtgagtgaaa ataattatat tgttcgaaga acagcgcttt ttggccggag
     2161 aaaagcagtt ctgcaaacga tggtatatcg atatgagttc ttttggcatt aatccaggca
     2221 agacgccggg caataagcgt aatattccgg gtgttactta tttccatttc tgtcaacagc
     2281 atcccccccc ccacgccgga gaataaaaaa gccgcctgtt ccagcggcaa gccctacatc
     2341 aagggaaact acgtcttacg attcgttttg aaccgggacg ctttgttgct gcgaatcact
     2401 aaccagataa tccacgtttt gtgagtgggt ttaggttaag gcttcaatag aacaataata
     2461 acgttaagtg attagggcaa cctgtgattg ctaaaatgat gaagtcttca catcatttcg
     2521 gtgaaggcga tatgtgttac accacgtatt gagagacctg catgcccttt acaggtgaac
     2581 gggaacggga atagagcggc gatttagcca gagatgggtt tcataacact gtaattcata
     2641 tcacaattat tataactggc ggtatttttt taacctttgc aattcgtttt atctgatgtt
     2701 tatgataatt ttcaaaaagt tggccccctg acatcaccgg gagccaaaaa aatacaccag
     2761 ttattattgt ggctatatta tttcatgagt aaacaaatat gtcaggcgta tatttgttta
     2821 ctcaaattgc ttgtttttta gtcatcgtga tgatgagcga gagaaaatcg atgttgatag
     2881 gttttttatg cttttgattt ttttatatgt ttgtattatt taaactgttt ttctgtattg
     2941 tgtagcattc cctttagatt tttatgccat cgtcgccgat ggctttttta ttccgttaag
     3001 gtaatttttg agttcatttg catgaacgac tgatgttctc catgttcgtt ttcactcttc
     3061 tttttctccc gcgaatttga tttatgtggt tgtgacatta gttatgagat agagccacca
     3121 gtgtgatgaa ctcacactac aaattcgttc agcactaaat cagaattcga tgcaaatctc
     3181 ggacgctgga tcatcacagt gatccttcta taacccggat ttgagagtcg caaacccata
     3241 ttagggcaaa gaaagcctaa tgcccccgtg gtgggtacac cagaaaacgg ggagcgaagc
     3301 gaccagcatc aggccgttcg tatgctgctt cgattttgtg ggtgttgcgc cagaccttgg
     3361 caatcacgcc tcaccgcaac cgctgccttc actctcactt tatcaggtac gttattggtt
     3421 aacgtgagac cgggtagagc cagcttcttt cccgagctgg tcaggttccc accgcttgcc
     3481 tgtttatcct gccggagtcc ggggcgtaga cctgagagtc aaggtgcaga tatgggttta
     3541 atgtcgttga atggtattaa gctggtagtg acattcctag ccagccctgc taggatttga
     3601 ttgcggtctg atccgagcag gtatctgcac gaattagagt actcaagccc tgtgattctg
     3661 tcacggggct tttgtacatc tagagccaga aaaatcatta tcactcagtc agatcaccgc
     3721 aacgcccgcc acagcttcat gaccgaacgt agtgagcgaa tgagcgagga ggcgtcattt
     3781 tcaccgtccg cccatcattc tgcttatgct tcgaagccgc attttcctct gcaacatagc
     3841 ccgatttccg tcaatttccg cacagtgtta aaatagataa gccagtatac actccgctag
     3901 cgctgcgtga ctggttcagg gctgcgcccc gaaacccgct aaaagccact gacgcgcctg
     3961 cggcttgtcc aacgccgcgc cgaccgggaa ggttttcccg cccgtcccgg cgttatcagg
     4021 agaccggttt gtcagagaaa cggaacaaga tgctcaccat gtgggtgacg gaggacgagc
     4081 accggcggct gcttgagcgc tgcgacggca ggcagctggc agcgtggatg cggcagacct
     4141 gcctggacga aaagccggca cgtaccggaa agcttccctc gatctcgccg gcgctgctgc
     4201 gccagcttgc cggcatgggc aacaacctga accagacagc ccgccgcgtt aacgccggcg
     4261 gcggtaccgg ccatgacagg gtgcagattg tggcggcgct gatggccatc gatgccggcc
     4321 ttgagcggct gcggcacgcc gttcttgaga aaggaacgga cgatgatcgt taaatttcat
     4381 ccacgcggtc gcggcggtgg cgccgggccg gttgactatc tgctgggtaa agaccggcag
     4441 cgtgaaggcg ccaccgtcct gcagggcaag ccggaagaag tccgggagct gattgatgcg
     4501 tcgccttacg tgaagaaata cacctccggc gtactgtcgt ttgcggaggc agatctgccc
     4561 cctggccagc gggaaaaact gatggccagc ttcgagcggg ttctgatgcc cgggctcgat
     4621 aaagatcagt acagcattct gtgggtggag cacgcggaca aggggcgtct ggagctgaac
     4681 tttcttatcc ccaataccga gctgctgacc ggcaggcggc tccagccgta ctacgaccgc
     4741 gccgaccggc cgcgcatcga tgcctggcag acggtggtca acggccggct ggggctgcac
     4801 gacccgaacg cgccggaaaa ccgccgggcg ctggtcacgc cgtccgggct gccgaaaacg
     4861 aaacaggagg ccgcagaagc cattacgcgc ggactgctga gcttagcctc gtccggggag
     4921 ctgaaaaacc gtcaggacgt cactgaggcg cttgagcggt caggttttga ggtggtacgc
     4981 accacgaaaa gcagtatcag cattgccgac ccggacgggg ggcgaaacat ccgactgaag
//
