LOCUS       NZ_CP136607             5933 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter portucalensis strain L2724hy plasmid p6-L2724hy,
            complete sequence.
ACCESSION   NZ_CP136607
VERSION     NZ_CP136607.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37738783
            Assembly: GCF_032917905.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Guo,K.
  TITLE     SFO-1, KPC-2, NDM-1 were first reported in Portuguese citrobacter
            collected clinically
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Guo,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2023) Basic Medicine, Zhejiang Chinese Medical
            University, Puyan Street, Hangzhou 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicylers v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:18:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,316
            CDSs (with protein)               :: 5,316
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 90 of 124
            Pseudo Genes (internal stop)      :: 25 of 124
            Pseudo Genes (multiple problems)  :: 24 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136607.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="L2724hy"
                     /host="Homo sapiens"
                     /db_xref="taxon:1639133"
                     /plasmid="p6-L2724hy"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-09-11"
     gene            complement(join(4795..5933,1..361))
                     /locus_tag="RY846_RS27655"
                     /old_locus_tag="RY846_27655"
     CDS             complement(join(4795..5933,1..361))
                     /locus_tag="RY846_RS27655"
                     /old_locus_tag="RY846_27655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="WP_000635827.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLE
                     LNFLIPNMELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL
                     PKTKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRN
                     IRLKGAIYEQSFNAGKGLRAEIESAAAEYRRDAESRIQRAREVCQSGTERKREENQRRH
                     PRPRPEAVLSHEPAHERHGAYGQPDVADHRPGVYTADSIGRKHSMVAGAADTRQLHDHP
                     GAEEHAGHAVREEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKT
                     VAERIRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAA
                     PVVTRLNVIEAHREQERAAQHQKALELERSQRQRTYNGPSL"
                     /name="nuclease"
     gene            complement(351..674)
                     /locus_tag="RY846_RS27660"
                     /old_locus_tag="RY846_27660"
     CDS             complement(351..674)
                     /locus_tag="RY846_RS27660"
                     /old_locus_tag="RY846_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2063..2356
                     /locus_tag="RY846_RS27665"
                     /old_locus_tag="RY846_27665"
     CDS             2063..2356
                     /locus_tag="RY846_RS27665"
                     /old_locus_tag="RY846_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052905524.1"
                     /translation="MTIVISKEIEDKLTNKHKVTIKEVHESLANFKGNLLIDNREQHRT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNELERKIYAKHGKKTA"
                     /name="hypothetical protein"
     gene            2554..2922
                     /locus_tag="RY846_RS27670"
                     /old_locus_tag="RY846_27670"
     CDS             2554..2922
                     /locus_tag="RY846_RS27670"
                     /old_locus_tag="RY846_27670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059931.1"
                     /translation="MKTTSEFARDAQKWDDRELGASEDHVEVASMAEMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAEKKQALRRELDKLNEREEMHQ
                     QEDTVPVDQFIESIRKQA"
                     /name="hypothetical protein"
     gene            3104..3658
                     /gene="aac(6')-Ib"
                     /locus_tag="RY846_RS27675"
                     /old_locus_tag="RY846_27675"
     ARG             3105..3655
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4152..4703
                     /locus_tag="RY846_RS27680"
                     /old_locus_tag="RY846_27680"
     CDS             4152..4703
                     /locus_tag="RY846_RS27680"
                     /old_locus_tag="RY846_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     ARG             2982..3658
                     /name="AAC(6')-Ib8"
                     /gene="AAC(6')-Ib8"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(776..962)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.34"
                     /coverage="87.0"
                     /evalue="2.7100000000000003e-47"
     Typing          complement(3..361)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.833"
                     /coverage="86.0"
                     /evalue="6.4899999999999996e-68"
     Typing          complement(4799..5931)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.063"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggagacgcct gccagtcagc agctccatgt tcgggatcag gaagttcagc tccagccgcc
       61 ccttatcctg gtgctcaacc cacagcacgc tgtactggtc tttatcgagt ccgggcatca
      121 gaacccgttc aaagctcgcc atcagctttt cgcgctgccc gggcggtaaa tccttttcgg
      181 caaaggacag gacgccggat gtgtactttt ttgcgtaggg cgaggcgtcg ataagctccc
      241 tgacttcttc cggcttcccc tgcaaaacgg tggcgccgtc gcgctgtcgg tctttcccca
      301 gcagataatc caccggaccg gcgccaccgc cgcgccctcg cgggtgaaac ttaacgatca
      361 tcgtctgcac ccttttccag cacggcatgc cgcagccgct cgagtccggc atctatggcc
      421 atcagcgcgg cgacgacctg cacgcggtcg tgcccgctgc caccaccggc gttaacctgc
      481 cgggcaatct ggttgaggtt gttccccatg ccggcaagct gacgaagcag cgccggcgag
      541 atcgagggaa gcttgccggc gcgggcgggt ttctcgtcca agcacgtctg gcgcatccag
      601 gctgcaagct gtttaccgtc gcagcgttcc aacagacggc ggtgctcatc ttcggtcacc
      661 cacatcgtga gcattttact gcgtttgtct gccaatccag cctcctgata acgccgggac
      721 gggcgggaaa tcttttcccg gtcggcgcgg tgttggacaa gccgcaggcg cgtcagtggt
      781 tttagcaggt ttcggggcgc agccctgaac cagtcaccta gcgctagcgg agtgtatact
      841 ggcttagtat gttacggcga gatggagatt caggaaagtg cttcatgtga gagggggaaa
      901 atgctgctgc agggagtagg tgcgaaatgc cggtgtcggt gacatgacgc ctcctcgctc
      961 actcggtcgc tacgctcctg ccgtgagact gcggcgggcg ttacgggctt acagataacg
     1021 ggatagcaag aagtcaggaa aggcggaaaa aggatgcggc gttgccgttt ttccataggc
     1081 ttcgccctcc tgacgagctt cacggaaatc agacgatggc cggtgctcaa atcagaaggg
     1141 gcgaaacctg acaggactta aagatcccca ccgtttccga tgggtcgctc cctcgtgcgc
     1201 tctcctgttc ccaccgtgag gtttacccgt tgcagaccgc cgtaaggtgg cgttttctca
     1261 ttccctccca tgcaactggg ttcctgttag gtggttcgct ccaagctgga ctatatgcac
     1321 gaactccccg tttagtccga ctaccacgtc cgatcaggta actgttctct tgattccaac
     1381 cctgacggac acgacaaatc gccactggcg gtagccaacg gtaactgaat tacggagcgg
     1441 gaaagtacac aatagaaaaa tcagagttct tgaagtgtca ccataactac ggctacactg
     1501 gaaggacagt tttggttact gcgctccgtt aaagccagtt accacggtta aaagatctgc
     1561 caggatcatt taaccttcga aaaaccacct gccagggtgg ttttttcgtt ttggatagaa
     1621 gagattacga cgactaacat atgatctcaa gaagatcctc aacttaagac tgtgcctttt
     1681 tccttttgct atctgcagcg cctttagcgc gacagcctct acaccacgtc tgaggggcta
     1741 cgttaccgcg taaatttcta aagccaaata gagtttcaac ttcttgatag gtcgaagcca
     1801 cgactgggtt taatcgacaa tcatagtcag cacaatgaca tggatgaggc aacgctattg
     1861 tattgctcat aatttctcct atatctatct ttcataacca acagcaatag gccaaaagca
     1921 gagaaaacgc agtttcctct cagtcgctgt atatacaaaa aaacagacaa taaccctacc
     1981 aacgctggac aaaattgaca agtgtaaatt ttgtatatac aataaatcca atttcacaac
     2041 aaaggccggg gcatcatgga atatgacaat tgtaatcagt aaagaaattg aagataaact
     2101 aaccaataaa cacaaggtaa cgataaaaga agtacatgaa agccttgcga attttaaagg
     2161 caacttgttg atagataaca gggagcagca ccggactaat cctccaacta actggtttat
     2221 cgctgaaact aaccagcgta gagaattaaa agtgtgtttc atgattatcg atggcgacat
     2281 tcatatcaaa acagctttcg agccaaacga attagaacgt aaaatctatg ctaagcatgg
     2341 aaagaagacg gcataacagc gctaaataag ttattatttt gcaagggctt acatgataag
     2401 cccttgcaaa aagcttacct cagcgaaaaa aaacaaaact aaaatgaagc tgaacctgat
     2461 ttctcaacat agaagttcct ttcggaacta gatcaagcat aaaaattgca gctaatcctg
     2521 atctgcagga aacagcacct agaggtctat acaatgaaaa ctaccagtga attcgcacgt
     2581 gatgcacaga aatgggatga tcgagagctc ggcgcctctg aggatcacgt tgaagtagca
     2641 tcaatggctg aaatgtcagc tttcaacgat gcattatcac tgcaagccat ctcaataaga
     2701 ttacagaaaa atcttgtaag cgatttaaaa tcgatagctg aaagctatgg tatcggttat
     2761 caaccaatgg ttagagatct tctgcaaaga tttgttattg cagaaaaaaa acaagcatta
     2821 cgtagagagt tagacaagct taatgaacga gaagaaatgc atcagcagga ggacacggtt
     2881 cctgttgatc agttcattga aagcataaga aaacaagcat aagaaaactt accatctaga
     2941 tacaaagaat ctcatcatca cgtttaaaag gaggattacc atgagcctta aacccggacc
     3001 aaaaagaatt gccgaatcga cggggcaacc tgatcaacgc caacgcgaca ataaaaagac
     3061 gcctggaaat actgacaagt taggcatcac aaagtacagc atcgtgacca acagcaccga
     3121 ttccgtcaca ctgcgcctca tgactgagca tgaccttgcg atgctctatg agtggctaaa
     3181 tcgatctcat atcgtcgagt ggtggggcgg agaagaagca cgcccgacac ttgctgacgt
     3241 acaggaacag tacttgccaa gcgttttagc gcaagagtcc gtcactccat acattgcaat
     3301 gctgaatgga gagccgattg ggtatgccca gtcgtacgtt gctcttggaa gcggggacgg
     3361 atggtgggaa gaagaaaccg atccaggagt acgcggaata gaccagtcac tggcgaatgc
     3421 atcacaactg ggcaaaggct tgggaaccaa gctggttcga gctctggttg agttgctgtt
     3481 caatgatccc gaggtcacca agatccaaac ggacccgtcg ccgagcaact tgcgagcgat
     3541 ccgatgctac gagaaagcgg ggtttgagag gcaaggtacc gtaaccaccc cagatggtcc
     3601 agccgtgtac atggttcaaa cacgccaggc attcgagcga acacgcagtg atgcctaacc
     3661 cttccatcga gggggacgtc caagggctgg cgcccttggc cgcccctcat gtcaaacgtt
     3721 gaaacccagt aaatcttcag gaaagtaagt tgtcagcaaa gcccttcggg gcttttttta
     3781 tacctgcagg tcggcatgat ttaccataac accccttgcc cgcaccgctc cggacgggtt
     3841 cagcgtgaaa gcggcgttag cccggtaaaa ccggcagtaa actccccgct aaaccgcgca
     3901 ttctgcgcct gataaatttc acgcgactgc atagccatgc agccacgtga atggctcatg
     3961 ctttcaatgt atatcctcgt ccaagcccct gttttaaccg gtttatcaaa aaatcaaaca
     4021 gaaagcctta ccactaacag taaaagaggt agggggctgt attttatcgc ataaaaaaca
     4081 tttacaacac gaattctatc gtatagattt cataaaaaaa tgaaatttac tcacctatta
     4141 agggtttcta tatgaaagat atgaaagctg ttgtggtttt taccgggaag gatttgaaca
     4201 taatgagaac tgaggggggg tcaggctact ggcatgccag aacagacagg ctcaacgatg
     4261 ccgattacct catagcagta agaaacagaa gggaaacgtg ggcagtaaaa gacctagaac
     4321 acggcacagc attcctgatc gccaaaatta ccgggtgttt taaaagccct gattacgacg
     4381 acaggaacgt cataaccttt gatgaatacg cagaaattca cacgccaaaa gcatggaaaa
     4441 tgctaaccga tggtcagcgc tatccggttg cttacctgag cgcacaggaa gcttttttac
     4501 gtattggggt aacaccggaa caactggaat ggaagaaatt ccatccttca tccccatcgg
     4561 ttcctaatac agtgattcct ggtctggctg aagagaaaac agaaaaactc tcccttaacg
     4621 aggcaattga aagagccaaa aaggatatta gcaacgcaac agggattgat agctcagcca
     4681 taacaataag catcaagatc tgatgaagcg tgaacaacaa gatagcagcc tcatgttcta
     4741 gaactgaacc tgtacgccag aaacagcaag cccatgcttt tccggcgcac agattcaaag
     4801 tgacggaccg ttgtacgttc gctgtcgctg agatcgttcc agctccagcg ctttctgatg
     4861 ctgcgccgcc cgttcctgct cacgatgtgc ctctatcacg ttcagccttg tgacaaccgg
     4921 cgcagctgct cgctcaaact ctgcacctgc ctgctcaagc ccgtgacgcg ctcgctcagc
     4981 gccgcgttct cccgttgcat aagaccagac atcgtccgcc attccgcgaa ggcgttctcc
     5041 cactcgtcca gccttttcga gtagtcctgc tgtagctgct ctaatgcgct cagcaactgt
     5101 ttttccagct ccgtcatgtg ctatttcccc gccagtatca tccagctcga atcctctccg
     5161 aaccgtcccg cttccgggtt caccctcacg cagcggcgtc tctgctctcc gcaggtcgag
     5221 agctgcacgc cgctgttcct ctcggacagc atggcctgcg tgctcttctg ctcccggatg
     5281 atcgtgtaat tgtcgagtat ctgctgcccc tgccaccata gaatgcttgc gcccgatgct
     5341 gtcagcagtg tagacaccag gacgatggtc agccacgtct ggctgaccat acgcaccatg
     5401 cctttcgtgc gctggctcat ggctgaggac agcttccggt cgtggtcgag gatggcggcg
     5461 ctgattctct tctcgctttc gctcagttcc gctctgacag acttctcgtg ctcgctgaat
     5521 gcggctttca gcatctcgcc ggtactctgc tgctgcgctt tcgatttctg ctctaagtcc
     5581 tttgccagcg ttaaaagact gttcatagat ggctcccttc agtcggatgt ttcgcccccc
     5641 gtccgggtcg gcaatgctga tgctgctttt tgtggtgcgt accacctcaa aacctgcgct
     5701 ttccagcgcc tcagtgacgt cctggcgggt tttcagctcc ccggacgagg ctaaggccag
     5761 taaaccccgt gtaattgcct gagcggcttc ctgcttcgtt ttcggcagcg cggacggcgt
     5821 gaccagcgcc cgccggtttt caggcgcgtt cgggtcatgc agccccagcc tgccgttcac
     5881 gatggtctgc caggcatcaa tgcgcggacg gtcggcgcgg tcgtaatacg gct
//
