LOCUS       NZ_CP136458             4167 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella pneumoniae strain Kpneu34 plasmid pKPNEU34_3, complete
            sequence.
ACCESSION   NZ_CP136458
VERSION     NZ_CP136458.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37703293
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Loop,J., Habermann,D. and Brinks,E.
  TITLE     ESBL producing Klebsiella spp. from sprouts
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Loop,J., Habermann,D. and Brinks,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2023) Department of Microbiology and
            Biotechnology, Max Rubner-Institut, Hermann-Weigmann-Strasse 1, Kiel
            24103, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 31-MAY-2023
            Assembly Method                   :: unicycler v. v.04.9b
            Assembly Name                     :: Kpneu34
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.53x
            Sequencing Technology :: Ilumina Miseq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096365.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:19:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kpneu34"
                     /isolation_source="sprouts"
                     /db_xref="taxon:573"
                     /plasmid="pKPNEU34_3"
                     /geo_loc_name="Germany: Kiel"
                     /collection_date="2017-11-27"
     gene            complement(306..548)
                     /locus_tag="RZC88_RS00005"
                     /old_locus_tag="RZC88_01540"
     CDS             complement(306..548)
                     /locus_tag="RZC88_RS00005"
                     /old_locus_tag="RZC88_01540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(914..1546)
                     /locus_tag="RZC88_RS00010"
                     /old_locus_tag="RZC88_01545"
     CDS             complement(914..1546)
                     /locus_tag="RZC88_RS00010"
                     /old_locus_tag="RZC88_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1546..2661)
                     /locus_tag="RZC88_RS00015"
                     /old_locus_tag="RZC88_01550"
     CDS             complement(1546..2661)
                     /locus_tag="RZC88_RS00015"
                     /old_locus_tag="RZC88_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(3006..3119)
                     /locus_tag="RZC88_RS00020"
                     /old_locus_tag="RZC88_01555"
                     /pseudo=""
     CDS             complement(3006..3119)
                     /locus_tag="RZC88_RS00020"
                     /old_locus_tag="RZC88_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3535..3726
                     /locus_tag="RZC88_RS00025"
                     /old_locus_tag="RZC88_01560"
     CDS             3535..3726
                     /locus_tag="RZC88_RS00025"
                     /old_locus_tag="RZC88_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3726..4127
                     /locus_tag="RZC88_RS00030"
                     /old_locus_tag="RZC88_01565"
     CDS             3726..4127
                     /locus_tag="RZC88_RS00030"
                     /old_locus_tag="RZC88_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3007..3414)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac
       61 ccacacccca cgcaaaatta aaattttgca gattccttat ttacaacaat gggttaatga
      121 aaaataaatg tgcggtgatg tgttacaaag tgcagatcca agtcgtggac gactctatgg
      181 cgaatcgctg acgaattgcc cacgattcat atgtgaatca cgctttatta tcatattgtt
      241 atgatgaatc gactcttgat tcaattcatc actttgggtg ttttttgctc atggtgactc
      301 ttgattcaat tcacgattcg aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg
      361 ccataacgtg attgccggcc gtcgacgaac ccgattcctg aaggtgctga aaaccggttt
      421 tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt
      481 tcggaggaga agaacgccgg aaacaggact tcccctggct gaatggtgtg aatttccgcg
      541 ctatgcactt gcgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg
      601 ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgt cctgagtccg
      661 ttttagcggt gcgtacaatt atgattttat gttaaattgt tttaagtgaa tagagagaat
      721 ataacttaag ggagcatctt gtgctgggtc ttttacatta gatctgcaca atcattgttt
      781 tggcatctgg tgatgtcttt aggacgtttg agattgctga taactaaagt gtatgatgct
      841 ttctatacgt tatccggcgt ttaataacac cctggtgata agccagcccg tgaagggctg
      901 gcttgatttg gatttatatt tttgaaataa aatcttttat ttcatcaaag caattgtctg
      961 ctgtgtttat tattccattt ttcatggata ttcctattct tgagtcggat tctctattgc
     1021 agctaagata gacttgtacc ttagcttttt tttcgtttgt tggataagtg tactctttgt
     1081 cactcgctga gatgtcatct ttattatttt taattttttc gaaaaattct attatgtatt
     1141 catataacgc tcgagtgctt gcgtgttcaa ttatcagatc ttcaatggca ccttgcttgt
     1201 cttttcctgg cattataaag aaagaaattt ttgtattgcc attcatagct atctcgttat
     1261 gcgagctggg ggaggagatt tgtagttctc gcaatgtggc aattattgat ctttctgtgt
     1321 cagaaaatga tgtgtcagca tctaatataa taaaaatact ttctttttca tcgaaagaat
     1381 caatgttttt gaatgctcta agtgcttgtt ttaattttga ttttccattt attttttcga
     1441 caaaaacatc ggtaatgcct tgtatttcaa gtaggttgca tagaaaatga aaatcatcat
     1501 caccctcaac gaaaatgatc tttttgctat tatttatgtc tttcattatc ttacctccca
     1561 gtcgttctca actgagtagg agaaatcatc catattgaat tgtacggctg ttggttttag
     1621 ggagttcttt tctcttccta gtctaatata agcgatatct tcatttttgc tatctatttc
     1681 gtttattgct cgtatcacat cttgactatg agttgttgca aatatttgat tgttgttttg
     1741 ttttgcagat gaaattaatg attttattat gtctttaatt actgagtaat gtattccgtt
     1801 ctcgatttca tcaataagaa taattgagtt ttcctgtacc agcactgagc tgataaaaga
     1861 taatgctcga ctgataccct cgccaagcat tgatatttca tttagctcac taaatccaat
     1921 gtctaaatat atttccttat tgttacctat cgcagatatt gctatatcta caattctgtc
     1981 atctattttt tttaagtttt ctagtatgtc ttttttcctt ttttgtgtta gtaggctaga
     2041 tacatttgta attgttgcct ctttgtttat ctttctggat gttgttatta ttgttgctgt
     2101 cttaaattta taattaatcc catccctttc gttttttatt tcggatttta tttctacgcc
     2161 atcttctttc aattgcatag agaaaagctt attggaaacc ctgtttttcg cattgggtgt
     2221 ctcggtcaca tttattttta aagagtttac tgtggcctga gaaccgatag tttgacgagg
     2281 tatgttttgg ttagatagta cggtgtcaac gtttagagac aaacttgatt ctaatctctg
     2341 atttgtttct gtttcatatg tttgtgttac ttcagagcgt tcatctcgaa tgactatgga
     2401 catcttttgt gatgtgtcca tatcatgaaa gtaactaacc caaaatggct tgtttttgtt
     2461 tatttctgcc atttcttttc tgaatgcttg aatgtttaac aaaacagccg ggttttttac
     2521 atcataaaaa caaaaaatag catccagtat agatgttttc ccgtagttgt ttttcccagc
     2581 tatgatattt aaagtcttta attgctttag atatagctca ttgaagcatt tgaagttctt
     2641 taaacttatt tcctttatca tgatagcctc tgttcgtgtt tttgttatat gccatacgat
     2701 ttgttagtac cttaataaaa tgtacagctt tcattgtgat tagctagact ttatgtcaaa
     2761 gattaacttt gattgcttgt gttttaatcg atttttttta ctttcttctt gagattatct
     2821 ggcagcagcc gtaagcttcc ggctgtgaac gaaaaaaacc gccttggcgg gcggtttttt
     2881 cgaaaggttc agtagagttg gcgctctatg aaccgaggta actggcttaa agggagcgca
     2941 gtcaccaaaa ctgttcgttt agtttagctt taactggcgt ataacttcaa gactaactcc
     3001 ctaaatcagt taccagtggc tgctgccagt ggcgctttgt cgtgtctttc cgggttggac
     3061 tcaagacgat agttaccgga ttaggcgcag cagtcggact gaacgggggg ttcgtgcata
     3121 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
     3181 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     3241 gagggagcca tcagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     3301 ctgatttgag cgtcagattc cgtgatgctc gtcagggggg cggagcctat ggaaaaacgg
     3361 ccgctgtgcg gcctttcctt ctttcctcca tgcgattact cggttttctc gagcgtttct
     3421 acgcatgctc ctgccgctcg ccgcagccga acggcaggag cgtagcgact gagtgagcga
     3481 ggaagcggaa aagaggagat ccctacactg accactgacg ctctgaggcc tcttatgacg
     3541 aaacaacaga aaacagcgct gaacatggct aaattcatcc agaatcagtc tctgctcctg
     3601 ctggagaagc ttaacgagct tgatctcgat gtcgaggccg acctttgtga gaaactccac
     3661 gatgatgcgg agcatctttt ccgtacactg tcctcgagac tggatgcatt acaggatggt
     3721 aactgatggc cagagtgtct attagtgagg ctgcccgact gacgggtaag agtagaacaa
     3781 cactgcacag gttaattaaa gcaggtgacc tgtccacctg tagcggtgag cgcaatgcaa
     3841 aaatggtaga tacttcagag cttttacgtg tctttggccc ctttgagcaa ccgaagggtg
     3901 aacaccattc tggacaggtc tctggacagc gtgatacagc ctctgctgac cagagtgaac
     3961 aggtgatccg tcatctgaaa caggagattg aacacctgaa caccttactt ttgtccaagg
     4021 attcgcacat tgacagcctg aaacaggcca tgctccttct tgaaaatcac caggcaaaat
     4081 taactgaatc cccggcgcca tggtggaaat tctggaaaaa atcctgaccc cgtcatgctt
     4141 cagttttcct ctccggccat cccaggg
//
