LOCUS       NZ_CP136357             5164 bp    DNA     circular CON 19-OCT-2023
DEFINITION  Enterococcus faecalis strain ES-1390 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP136357
VERSION     NZ_CP136357.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37639063
            Assembly: GCF_032681025.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Yoon,J.W. and Lee,J.B.
  TITLE     Complete genome sequences of Enterococcus faecalis isolated from
            human patients in South Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Yoon,J.W. and Lee,J.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2023) Department of Veterinary Medicine, Kangwon
            National University, 1 Gangwondaehakgil, Chuncheon, Gangwon 24341,
            South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: De novo assembly using Unicycler v. 5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 479x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/18/2023 16:58:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,889
            CDSs (total)                      :: 2,812
            Genes (coding)                    :: 2,764
            CDSs (with protein)               :: 2,764
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 20 of 48
            Pseudo Genes (incomplete)         :: 28 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 8 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136357.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="ES-1390"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed4"
                     /geo_loc_name="South Korea:Gyeonggi-do, Anseong-si"
                     /lat_lon="37.0062 N 127.271 E"
                     /collection_date="2022-01-26"
                     /collected_by="Nguyen Thi Mai Tho, Jun Bong Lee"
     gene            complement(401..697)
                     /locus_tag="RX141_RS14425"
                     /old_locus_tag="RX141_14425"
     CDS             complement(401..697)
                     /locus_tag="RX141_RS14425"
                     /old_locus_tag="RX141_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002404941.1"
                     /translation="MGLMTGYNMTKEEYEITQAVLTRLDKFESELESLLLDLEPLDLSR
                     LDTVVREETLRMFNYLSRSYILLLDTVAYKDTLEEDISSGIVKIDGQPLDEEE"
                     /name="hypothetical protein"
     gene            complement(825..1556)
                     /locus_tag="RX141_RS14430"
                     /old_locus_tag="RX141_14430"
     CDS             complement(825..1556)
                     /locus_tag="RX141_RS14430"
                     /old_locus_tag="RX141_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_025188181.1"
                     /translation="MNEKSELAVKYQNELNLVALKQFNAKEMNLFFALCARMKDKGLDS
                     IIFSFEELKELSDYKMTAIKAFTNDLDSLYSKMLQLTYRNEYDDDGSFRRFVLFTGFDV
                     NVKKQTVEVSINPKLEGILNGLTTEFSRFELSAFTAIRSTYAKTLFRLLMQYRSTGYYT
                     VSIDEFKEIMDIPKSYTQMGQIDQRVIKPAMKELNNYFENLELTKIKAKKGNKIAKLEF
                     TFTGLKTNKPSITMHDWVNGE"
                     /name="replication initiation protein"
     gene            2211..2297
                     /locus_tag="RX141_RS14435"
                     /old_locus_tag="RX141_14435"
     CDS             2211..2297
                     /locus_tag="RX141_RS14435"
                     /old_locus_tag="RX141_14435"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2817..3017)
                     /locus_tag="RX141_RS14440"
                     /old_locus_tag="RX141_14440"
     CDS             complement(2817..3017)
                     /locus_tag="RX141_RS14440"
                     /old_locus_tag="RX141_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(3106..4785)
                     /gene="mobV"
                     /locus_tag="RX141_RS14445"
                     /old_locus_tag="RX141_14445"
     CDS             complement(3106..4785)
                     /gene="mobV"
                     /locus_tag="RX141_RS14445"
                     /old_locus_tag="RX141_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_104659816.1"
                     /translation="MSKIVARMEKMKVGNLSGIQRHNQRETDNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQSLLEIQEELPN
                     YLKERGYAIERGLRGSPQKHLSVKEYKDVQKEIQVSKQELANQEQILVEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANIKEKKFSFNGLK
                     YVLSASDLSAIKSITKDFETLRTQNKHLKIENNTLAQKNKELTQVNHEQELQITDLAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDRTTQKVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     Typing          complement(3110..4845)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.189"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgagtgcta aaaatgtgct agtttacctg aaagtacctg ctagtaaagt gctagcgaag
       61 aacgaaagtg taccccacgg gaaagccttg cactgcaagg cgtcccacct tgtgtgggac
      121 ccagtttccc ttatgctctt ccgaaacaca tgaaacaatt aagtaaattt caagaacaaa
      181 agacaaaaaa taacaacagt ggggattggg ggaaccccaa aaatttgaat gacacctacc
      241 aaaaatactg gtaggtgtca ttcaaaatat cgcaacatgg taccatgttg ctctgctcgc
      301 aaaaagaaag cctaccccaa aatggaagta gactaatctt gatatttttc gagtgtaccc
      361 cgaatatcta atactttttt ataacaatca gacctagtct ttattcttct tcgtctagag
      421 gctgaccgtc aattttcacg atcccactgg aaatatcttc ttctagcgta tctttgtagg
      481 cgaccgtatc aagaagtaga atgtacgacc gacttagata attaaacatt cttagcgttt
      541 cttctcggac gacagtatct aatcgtgata ggtcgagtgg ctctaaatca agtaataatg
      601 attctagttc actttcaaac ttatctagtc gagtcagtac agcctgtgtg atttcgtact
      661 cctcttttgt catgttgtat cctgtcatta atcccatgta tatgacctcc aaaataattt
      721 attttagaag tcctcacgca aatatgctaa aataatctag cagtcgtagt ggattgcgtg
      781 aggaacttgc taacttgctt aatgtgaggg ggcaagttct ttttttattc tccgtttacc
      841 cagtcatgca tagttatgct tggtttgttg gttttcagac cagtaaacgt aaattctagt
      901 ttggcaatct tattgccttt tttggctttg atttttgtta attctaaatt ctcaaagtaa
      961 ttattgagtt ctttcattgc aggttttatc actctttggt ctatttgtcc catttgagta
     1021 tacgattttg gaatatccat tatttcttta aattcatcaa tactaacggt atagtaacca
     1081 gttgaccgat attgcattaa taagcgaaac aacgttttag cataggttga tcggatagca
     1141 gtaaatgcag ataactcgaa cctactaaat tcagttgtta agccattaag aataccttcc
     1201 aatttaggat tgatacttac ttcaactgtt tgttttttta cattaacgtc aaagccagta
     1261 aacaagacaa atcgtcgaaa acttccgtca tcatcatact cattccgata agttagttgc
     1321 agcattttac tgtataggct atctaaatca tttgtaaacg ccttaattgc agtcatttta
     1381 tagtcgctta attcttttaa ctcttcaaaa gaaaatatga tactgtctaa acctttgtct
     1441 ttcattcttg cacataaggc aaaaaataag ttcatttctt tagcattgaa ctgttttaaa
     1501 gcaactagat ttaattcgtt ttgatattta acagctaatt ccgatttttc attcattcct
     1561 ttcgccccct cacgctcaca ttatataaac agcatacatt aatgtcaagt atgctgttta
     1621 tataatgtga gcgtgatttt acaaagataa tttgttatta gtagtcagat aaaatgatgt
     1681 tggtacacag ataaaatgat gttggtacac agataaaatg atgttggtac acagataaaa
     1741 tgatgttggt accctctgct cttccttaga gccacgtggc acacagaccg cctaaaaagg
     1801 ttttaaaaaa tatataaaaa atatataaaa ataaacgcgt gcgcgtgcga ggaaatttcc
     1861 cgcctaaaaa ttcactcttt tttctacaaa caggttcgcg atatttacga aactgtcact
     1921 aaaaaattga aaaacaaaaa aaggagtgag cgaaattctt cgctcacatt atgcttgcag
     1981 cgttcacttc gttcactcgc taaaatcaaa atcaataccg ttgacaaggt aactcatttg
     2041 gttaagctct atttgcttgt ctgaagctct ttaagacgtg tttagtggtt tcaggtaatc
     2101 ttgttaagat tgcgccttgc aggcggtcag ggcaagccct aacaaccccc acaagaaatt
     2161 cttcttgtga tatacttgtt ttggcaactg ccccgaaagg gggtgtaaac atgattgaaa
     2221 gcttaatttc gttgattatc gccccacttt ttgtgggttt agtacttttg ctagttgatc
     2281 gttggctaga cgattagcag ataatctatg cagttgcttt tctagctcca acgctgaaat
     2341 cgcaacagtg taaaaaaagc cctcctactg agaataggag ggctttttgg tgtaaacatg
     2401 attttgctta atttcgcttc gcacttacac aataacattt tttttctgta ttttcaaacg
     2461 tcagattaga ttacgcaaaa cgtcaattag agaaatacca tatcgaaata gttagtatta
     2521 aacatagtaa tactatgagg atagacaata ttttactttt tggactatct tcaaagcttt
     2581 tattttctgg tctgtaagtg ggattcacta ctttcgcgtt gtaatccatt gtacttccca
     2641 aatatacttc tgaaggaatt aaaaaaaata ataaaattcc gtaaaaaata attatcaata
     2701 tgggcaaatc aatattaact aaaaaagaag ttatatagct taaaactaaa ggaatagtaa
     2761 tataaaatat attttttatt agatattttt tcataaaata acctcaaatt tagcaactat
     2821 tttgtccatc ttgtatgcat tgggatacaa aatccaccgg ctttatgata gcctcctgca
     2881 gtccacccgc cttttttcca ataaaatcct ggctgacact ctatagaatc ccactctcta
     2941 gcacttgctt ctgtagttga acttaaagca acagagataa aagacacacc taaaattaga
     3001 actagcaact ttttcataat gcttacatca ctttttttta atttttatta atatatttta
     3061 atttaactgt ttttatgtgt caataattta tcaattaaat tatttttaac gagataaacc
     3121 tcgtcctttt gacatatctc ttgaatattt tttactctgt tgccaagcct ttttatctgt
     3181 cgactgttca aacgtaaaag ctttcttctc tagcacttgt tcttcagaca gctgtaaagt
     3241 attagttcgt gaacggccat ttgccatata ctcgccgaca aactcataac accctgtttc
     3301 tctgttgtaa aacggggctt ttagggggta gaaatgtggg tttaaggaca ttgttacccc
     3361 ttttgacccc gtatctggtg gtaaaaacaa tgtccctttt tcgtcaatga ggtaggttct
     3421 gtccttggaa tatcgctcca gggatttttt taatttaggc gcgttctgaa ggtacttcac
     3481 tccttgctga atggtaaata aaatggtttc tttggcctgt cctagtctct catgaaggta
     3541 agtggttaac ccgtcaaact ttagttttat ctcttgatat ttttcgttta gttcagaaaa
     3601 gtcgtaggtt aagtcagtaa ttttatcttt taaccgctta ttctctgttt ctaacacaga
     3661 aactttttgt gttgttctat caagttcttg tttgatttgt tcgctctggt catagtttag
     3721 ttgtttttct tcaaacgctg ttaattgttc ttttttcagg gttaattcat aggctaagtc
     3781 cgttatttgt aattcttgtt catgatttac ttgtgtcaat tctttatttt tctgcgccaa
     3841 ggtattgttt tcgattttaa ggtgcttatt ttgcgttcta agcgtttcaa aatctttcgt
     3901 aatacttttt attgccgaaa ggtcagaagc gcttagaacg tattttaaac cgttaaaact
     3961 aaatttcttt tctttgatat ttgctaaatc aggtaaaacg gttgtttgta gagagttaat
     4021 gatttcgttt cgcttcgttt ccagttggtt catggcttgg actttgccct ttacttcttc
     4081 gttcttctct tcaagaagac ggtcaatacg gaagattgcc tgttcatagg aatggagttc
     4141 tctttctttt tctaccagaa tttgttcctg attcgcgagt tcttgtttag aaacctgtat
     4201 ttctttttga acgtctttgt attctttgac agataaatgc ttttgcggac tcccacggag
     4261 tccacgctca atagcgtagc cccgttcttt caaatagttt ggcagttctt cctgaatctc
     4321 taacaaactt tgccgactaa acacctgttt actgctcaat cgcccttcgt acataggcac
     4381 aattcctaag tgcatatggg gcgtggtttc gtctaaatgt accgtggcat aggcgacatt
     4441 ttgtcgaccg cagcgatcag agaaatacgc gacaacatcg gtaaaaaatg cttgtgtgtc
     4501 tgtattctct tggaaaaagg cggtatcact agtgattatc cattcgttca caaggactgc
     4561 gtcttttcta accgctcgtt tactgattcg ttggctctca atgatttgtt tgattttctc
     4621 ccgataattg attgaaccag gattgactaa gtcataattc aagtgagatt tctcaatatc
     4681 aatatcagga ttggaatgat tatcggtttc tcgttgatta tgccgttgga taccgcttaa
     4741 gtttccaact ttcattttct ccatacgtgc gacaattttt gacatgagcg ctcccccccc
     4801 cttttttttc ttgtggcgtt ctttgtcttt ggatttcggc agcacttcca agtaaagtat
     4861 aacgcactat actttacttc gtaaaggtgc gctctgccga gggctgtcgg ctagtactcg
     4921 gaaggtcagc ctttaggctt cccaatctcg cacttagccg attgcctacg gtcaaacttt
     4981 ccatttctgc taaggcagaa actccaaatt cccctccgtc aaaaaaccgt ttaaataacg
     5041 actgcctgaa attagtgaac gtgctagctt tctgctagcc ggtgctagta atgtgctaat
     5101 ttggtgctaa aaacctgcta gtcactgcta ggaatctgct agtttgggtg ctagaaacct
     5161 gcta
//
