LOCUS       NZ_CP136350             5129 bp    DNA     circular CON 19-OCT-2023
DEFINITION  Enterococcus faecalis strain ES-1276 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP136350
VERSION     NZ_CP136350.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37639061
            Assembly: GCF_032680965.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Yoon,J.W. and Lee,J.B.
  TITLE     Complete genome sequences of Enterococcus faecalis isolated from
            human patients in South Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Yoon,J.W. and Lee,J.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2023) Department of Veterinary Medicine, Kangwon
            National University, 1 Gangwondaehakgil, Chuncheon, Gangwon 24341,
            South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: De novo assembly using Unicycler v. 5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 439x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/18/2023 17:14:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,928
            CDSs (with protein)               :: 2,928
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 25 of 71
            Pseudo Genes (internal stop)      :: 23 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136350.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="ES-1276"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed4"
                     /geo_loc_name="South Korea:Gyeonggi-do, Anseong-si"
                     /lat_lon="37.0062 N 127.271 E"
                     /collection_date="2021-12-31"
                     /collected_by="Nguyen Thi Mai Tho, Jun Bong Lee"
     gene            467..553
                     /locus_tag="RX139_RS15365"
                     /old_locus_tag="RX139_15365"
     CDS             467..553
                     /locus_tag="RX139_RS15365"
                     /old_locus_tag="RX139_15365"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1073..1273)
                     /locus_tag="RX139_RS15370"
                     /old_locus_tag="RX139_15370"
     CDS             complement(1073..1273)
                     /locus_tag="RX139_RS15370"
                     /old_locus_tag="RX139_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(1362..3041)
                     /gene="mobV"
                     /locus_tag="RX139_RS15375"
                     /old_locus_tag="RX139_15375"
     CDS             complement(1362..3041)
                     /gene="mobV"
                     /locus_tag="RX139_RS15375"
                     /old_locus_tag="RX139_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_172503054.1"
                     /translation="MSKIVARMEKMKVGNLSGIQRHNQRETDNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQSLLEIQEELPN
                     YLKERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLEAKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDLSAIKSITKDFETLRTQNKHLKIDNNTLTQENKELKQVNHVQELQITDLTHE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDKTTQKVSVLETDNKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            complement(3817..4113)
                     /locus_tag="RX139_RS15380"
                     /old_locus_tag="RX139_15380"
     CDS             complement(3817..4113)
                     /locus_tag="RX139_RS15380"
                     /old_locus_tag="RX139_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364284.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLTRLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLLDTVVYKDTLEEDIACGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(4241..4969)
                     /locus_tag="RX139_RS15385"
                     /old_locus_tag="RX139_15385"
     CDS             complement(4241..4969)
                     /locus_tag="RX139_RS15385"
                     /old_locus_tag="RX139_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002364286.1"
                     /translation="MKKDTELTVKYRNELNMITLKKFNAKEMDLFFALCARMKDKGLNN
                     IVFSFEELKELSDYRTTAIKAFTDDLDSLYSKMLQLTYRDEYDDDGSFRRFVLFTSFDV
                     NVKKQTVEVSINPKLEGILNGLTTEFSKFELSAFTSLRSTYAKTLFRLLMQYRSTGYYV
                     VNIEEFRELLNIPDYYQMGNIDQKVLKPAMKELNNYFEDLEITKIKAKKGNKIAKLEFT
                     FTGLKTTKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     Typing          complement(1366..3062)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.29"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagttgtt agtactctcc gctcttcctt agagccacgt ggcccacaga ccgcctaaaa
       61 aggttttaaa aaatatataa aaaatatata aaataaacgc gcgtgcgcgc gaggaaattt
      121 cccgcctaaa aatttcctct tttttctaca aacaggttcg cgatatttac gaaactgtca
      181 ctaaaaaatc gaaaaacaaa aaaaggagtg agcgaagttc ttcgctcaca ttatgcttgc
      241 agcgttcact tcgttcactc gctaaaatca aaatcattta ttaaggaact ctttgtatag
      301 tctctatttg cttgtctgaa gctctttaag acatgtttag ttgttttagg gtatcttggt
      361 taggattgcg ccttgcaggc ggtcagggca agccctaaca acccccacaa gaaattcttc
      421 ttgtgatata cttgttttag caactgcccc gaaagggggt gtaaacatga ttgaaagctt
      481 aatttcgttg attatcgccc cactttttgt gggtttagta cttttgctag ttgatcgttg
      541 gctagacgat tagcagataa tctatgcagt tgcttttcta gctccaacgc tgaaatcgca
      601 acagtgtaaa aaaagccctc ctactgagaa taggagggct ttttggtgta aacatgattt
      661 tgcttaattt cgcttcgcac ctaaacaata acattttttt tctgtatttt caaacgtcag
      721 attagattac gcaaaacgtc aattagagaa ataccatatc gaaatagtta gtattaaaca
      781 tagtaatact atgaggatag acaatatttt actttttgga ctatcttcaa agcttttatt
      841 ttctggtctg taagtgggat tcactacttt cgcgttgtaa tccattgtac ttcccaaata
      901 tacttctgaa ggaattaaaa aaaataataa aattccgtaa aaaataatta tcaatatggg
      961 caaatcaata ttaactaaaa aagaagttat atagcttaaa actaaaggaa tagtaatata
     1021 aaatatattt tttattagat attttttcat gaaataacct caaatttagc aactattttg
     1081 tccatcttgt atgcattggg atacaaaatc caccggcttt atgatatcct cctgcagtcc
     1141 acccgccttt tttccaataa aatcctggct gacactctat agaatcccac tctctagcac
     1201 ttgcttctgt agttgaactt aaagcaacag agataaaaga cacccctaaa attagaacta
     1261 acaacttttt cataatgctt acctcacttt tttttaattt ttattaatat attttaattt
     1321 aactgttttt atgtgtcaat aatttatcaa ttaaattatt tttaacgaga taaacctcgt
     1381 ccttttgaca tatctcttga atatttttta ctctgttgcc aagccttttt atctgtcgac
     1441 tgttcaaacg taaaagcttt cttctctagc acttgttctt cagacagctg taaagtattt
     1501 gtgcgcgaac gtccattagc catatactcg ccgacaaact cataacaccc tgtttctctg
     1561 ttgtaaaacg gggcttttag ggggtagaaa tgtgggttta aggacattgt tacccccttt
     1621 gaccccgtat ctggtggtaa aaacaatgtc cctttttcgt caatgaggta agttctatcc
     1681 ttggaatatc gctccaggga tttttttaat ttaggcgcgt tctgaaggta cttaactcct
     1741 tgctgaatgg taaataaaat ggtttctttg gcctgtccta gtctttcatg aaggtaagtg
     1801 gttaacccgt caaactttaa ttttatttct tgatattttt cgtttagttc agaaaagtcg
     1861 taggttaagt cagtaatttt atcttttaac cgcttattat ctgtttctaa cacagaaact
     1921 ttttgtgttg ttttatcaag ttcttgtttg atttgttcgc tctggtcata gtttagttgt
     1981 ttttcttcaa acgctgttaa ttgctctttt tttagggtca attcatgggt taagtccgtt
     2041 atttgtaatt cttgtacatg atttacttgt ttcaattctt tattttcctg tgtcaaggta
     2101 ttgttatcga ttttaaggtg cttattttgc gttctaagcg tttcaaaatc tttggtaata
     2161 ctttttatcg cagaaaggtc agaagcgctt aaaacgtatt ttaaaccgtt taaactaaat
     2221 ttcttttctt tgacatttgc taaatctggt aaaacggttg tttgtagaga gttaatgatt
     2281 tcgtttcgct tcgcttccag ttggttcatt gcttggactt tgccctttac ttcttcgttc
     2341 ttctcttcaa gaaggcggtc aatacggaag attgcttgtt cataagaatg gagttctctc
     2401 tctttttctg ccagaatttg ttcctgatta gctagttcct gcttagaaac ctgtagctcc
     2461 ttttgaacgt ctttgtattc tttgacagat aaatgctttt gcggactccc acggagtcca
     2521 cgctcaatag cgtagccccg ttctttcaaa tagtttggca gttcttcctg aatctctaac
     2581 aaactttgtc gactaaacac ctgtttactg ctcaatcgcc cttcgtacat aggcacaatt
     2641 cctaagtgca tatggggcgt ggtttcgtct aaatgtaccg tggcataggc gacattttgt
     2701 cgaccgcagc gatcagagaa atacgcgaca acatcggtaa aaaaagcttg tgtgtctgta
     2761 ttctcttgaa aaaaagcggt atcactagtg attatccatt cgttcacaag gactgcgtct
     2821 tttctaaccg ctcgtttact gattcgttgg ctctcaatga tttgtttgat tttctcccga
     2881 taattgattg aaccaggatt gactaagtca taattcaagt gagatttctc aatatcaata
     2941 tcaggattgg aatgattatc ggtttctcgt tgattatgcc gttggatacc gcttaagttt
     3001 ccaactttca ttttctccat acgtgcgaca atttttgaca tgagcgctcc cccccttttt
     3061 tttcttgcgg cgttctttgc ctttggattt cggcagtact tccaagtaaa gtataacgca
     3121 ctatacttta cttcgtaaag gtgcgctctg ccgagggctt tcgtctagca ctcgaaaggt
     3181 cagcctttag gcttcccaat ctcgtactta gccgattgcc tacggtcaaa ctttccattt
     3241 ctgctaaggc agaaactcca aatttccctc cgtcaaaaaa ccgtttaaat aacgactgcc
     3301 tgaaattagt gaacgtgcta gctttctgct agccggtgct agtaatgtgc taatttggtg
     3361 ctaaaaacct gctagtcact gctaggaatc tgctagtttg ggtgctagat acctgctagt
     3421 gagtgctaaa aatgtgctag tttacctgaa agtacctgct agtaaagtgc tagcgaagaa
     3481 cgaaagtgta ccccacggga aagccttgca ctgcaaggcg gggcaccttg tgtgcccccc
     3541 agtttccctt atgctcttcc gaaacacagg aagaaattaa gcaaatttca acagtaaaag
     3601 caaaaaataa cagcagtggg attgggggaa ccccaaaaat ttgaacgact cctaccagta
     3661 tttttggtag gggtcattca aaatatcgca acatgctagc atattgctct gctcgcaaaa
     3721 agaaagccta ccccaaaatg gaagtaggct aatcttgatg tttttcgagt attatcccaa
     3781 atagtcaaca ttttttataa caagcaaggc tagtttttat tcttcttcgt ctagtgattg
     3841 gccgtcaatc ttcacgattc cacaggcgat atcttcttct agcgtatctt tgtagacgac
     3901 cgtatcaaga agtagaatgt atgaccgact tagataatta aacattctta gcgtttcgtc
     3961 tcgaactaca gtatctaatc gtgataagtc aagcggctcc aaatcaagca atagcgcttc
     4021 tagttcactt tcaaacttat ctagtcgagt cagtacagcc tgtgtgactt cgtactcctc
     4081 ttttgtcatg ttgtatcctg tcattaatcc catgtatatg acctccaaaa taatttattt
     4141 tagaagtcct cacgcaaata tgctaaaata atctagcagt cgtcgaggat tgcgtgagga
     4201 gcttgctaac ttgcttaatg tgagggggca agttcttttt ttattctccg tttacccagt
     4261 catgcatagt tacgcttggt ttggtggttt tcaaaccagt aaacgtaaat tctagtttgg
     4321 caatcttatt gccttttttg gctttgattt ttgttatttc taaatcttca aagtagttat
     4381 tgagttcttt cattgctggt tttaaaactt tttgatcaat atttcccatt tgataataat
     4441 cgggaatatt caataattct ctgaactctt caatgttaac tacgtaatat cctgttgacc
     4501 gatactgcat taacaaacga aacaaagttt ttgcataggt tgagcgtaac gaagtaaatg
     4561 ctgataattc aaatttacta aattcagttg ttaaaccatt aagaatacct tctaacttag
     4621 ggtttatact tacttcaact gtttgttttt ttacgttaac gtcaaagcta gtaaataaaa
     4681 cgaatcgtct aaagcttccg tcatcatcat attcgtcacg atatgttagt tgtaacattt
     4741 tactgtacaa actatctaaa tcatctgtga acgcttttat cgcagttgtt ctatagtcgc
     4801 ttaattcttt caactcttca aacgaaaaaa caatgttatt taagccttta tctttcatgc
     4861 gtgcacataa agcaaagaat aggtccattt ctttagcgtt gaactttttt aaggtaatca
     4921 tgtttaattc gtttcgatat ttaactgtta attccgtgtc tttcttcatc ctttcgcccc
     4981 ctcacactat gaattttaaa acaaacaacc aactttgtca acggttggtt gtttacagag
     5041 aaaaagttgt tagtactcag agaaaaagtt gttagtactc agagaaaaag ttgttagtac
     5101 tcagagaaaa agttgttagt actcagaga
//
