LOCUS       NZ_CP136345             5107 bp    DNA     circular CON 19-OCT-2023
DEFINITION  Enterococcus faecalis strain ES-397 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP136345
VERSION     NZ_CP136345.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37639060
            Assembly: GCF_032680945.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Yoon,J.W. and Lee,J.B.
  TITLE     Complete genome sequences of Enterococcus faecalis isolated from
            human patients in South Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Yoon,J.W. and Lee,J.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2023) Department of Veterinary Medicine, Kangwon
            National University, 1 Gangwondaehakgil, Chuncheon, Gangwon 24341,
            South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: De novo assembly using Unicycler v. 5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 339x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/18/2023 16:57:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,254
            CDSs (total)                      :: 3,175
            Genes (coding)                    :: 3,093
            CDSs (with protein)               :: 3,093
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 37 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136345.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="ES-397"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed4"
                     /geo_loc_name="South Korea:Gyeonggi-do, Anseong-si"
                     /lat_lon="37.0062 N 127.271 E"
                     /collection_date="2021-07-05"
                     /collected_by="Nguyen Thi Mai Tho, Jun Bong Lee"
     gene            complement(335..421)
                     /locus_tag="RX142_RS16250"
                     /old_locus_tag="RX142_16250"
     CDS             complement(335..421)
                     /locus_tag="RX142_RS16250"
                     /old_locus_tag="RX142_16250"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            1026..1754
                     /locus_tag="RX142_RS16255"
                     /old_locus_tag="RX142_16255"
     CDS             1026..1754
                     /locus_tag="RX142_RS16255"
                     /old_locus_tag="RX142_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002364286.1"
                     /translation="MKKDTELTVKYRNELNMITLKKFNAKEMDLFFALCARMKDKGLNN
                     IVFSFEELKELSDYRTTAIKAFTDDLDSLYSKMLQLTYRDEYDDDGSFRRFVLFTSFDV
                     NVKKQTVEVSINPKLEGILNGLTTEFSKFELSAFTSLRSTYAKTLFRLLMQYRSTGYYV
                     VNIEEFRELLNIPDYYQMGNIDQKVLKPAMKELNNYFEDLEITKIKAKKGNKIAKLEFT
                     FTGLKTTKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            1882..2178
                     /locus_tag="RX142_RS16260"
                     /old_locus_tag="RX142_16260"
     CDS             1882..2178
                     /locus_tag="RX142_RS16260"
                     /old_locus_tag="RX142_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364284.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLTRLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLLDTVVYKDTLEEDIACGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            2954..4633
                     /gene="mobV"
                     /locus_tag="RX142_RS16265"
                     /old_locus_tag="RX142_16265"
     CDS             2954..4633
                     /gene="mobV"
                     /locus_tag="RX142_RS16265"
                     /old_locus_tag="RX142_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_172503054.1"
                     /translation="MSKIVARMEKMKVGNLSGIQRHNQRETDNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQSLLEIQEELPN
                     YLKERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLEAKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDLSAIKSITKDFETLRTQNKHLKIDNNTLTQENKELKQVNHVQELQITDLTHE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDKTTQKVSVLETDNKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            4722..4922
                     /locus_tag="RX142_RS16270"
                     /old_locus_tag="RX142_16270"
     CDS             4722..4922
                     /locus_tag="RX142_RS16270"
                     /old_locus_tag="RX142_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     Typing          2934..4630
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.29"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 caatggatta caacgcgaaa gtagtgaatc ccacttacag accagaaaat aaaagctttg
       61 aagatagtcc aaaaagtaaa atattgtcta tcctcatagt attactatgt ttaatactaa
      121 ctatttcgat atggtatttc tctaattgac gttttgcgta atctaatctg acgtttgaaa
      181 atacagaaaa aaaatgttat tgtttaggtg cgaagcgaaa ttaagcaaaa tcatgtttac
      241 accaaaaagc cctcctattc tcagtaggag ggcttttttt acactgttgc gatttcagcg
      301 ttggagctag aaaagcaact gcatagatta tctgctaatc gtctagccaa cgatcaacta
      361 gcaaaagtac taaacccaca aaaagtgggg cgataatcaa cgaaattaag ctttcaatca
      421 tgtttacacc ccctttcggg gcagttgcta aaacaagtat atcacaagaa gaatttcttg
      481 tgggggttgt tagggcttgc cctgaccgcc tgcaaggcgc aatcctaacc aagataccct
      541 aaaacaacta aacatgtctt aaagagcttc agacaagcaa atagagacta tacaaagagt
      601 tccttaataa atgattttga ttttagcgag tgaacgaagt gaacgctgca agcataatgt
      661 gagcgaagaa cttcgctcac tccttttttt gtttttcgat tttttagtga cagtttcgta
      721 aatatcgcga acctgtttgt agaaaaaaga ggaaattttt aggcgggaaa tttcctcgcg
      781 cgcacgcgcg tttattttat atatttttta tatatttttt aaaacctttt taggcggtct
      841 gtgggccacg tggctctaag gaagagcgga gagtactaac aactttttct ctgagtacta
      901 acaacttttt ctctgagtac taacaacttt ttctctgagt actaacaact ttttctctgt
      961 aaacaaccaa ccgttgacaa agttggttgt ttgttttaaa attcatagtg tgagggggcg
     1021 aaaggatgaa gaaagacacg gaattaacag ttaaatatcg aaacgaatta aacatgatta
     1081 ccttaaaaaa gttcaacgct aaagaaatgg acctattctt tgctttatgt gcacgcatga
     1141 aagataaagg cttaaataac attgtttttt cgtttgaaga gttgaaagaa ttaagcgact
     1201 atagaacaac tgcgataaaa gcgttcacag atgatttaga tagtttgtac agtaaaatgt
     1261 tacaactaac atatcgtgac gaatatgatg atgacggaag ctttagacga ttcgttttat
     1321 ttactagctt tgacgttaac gtaaaaaaac aaacagttga agtaagtata aaccctaagt
     1381 tagaaggtat tcttaatggt ttaacaactg aatttagtaa atttgaatta tcagcattta
     1441 cttcgttacg ctcaacctat gcaaaaactt tgtttcgttt gttaatgcag tatcggtcaa
     1501 caggatatta cgtagttaac attgaagagt tcagagaatt attgaatatt cccgattatt
     1561 atcaaatggg aaatattgat caaaaagttt taaaaccagc aatgaaagaa ctcaataact
     1621 actttgaaga tttagaaata acaaaaatca aagccaaaaa aggcaataag attgccaaac
     1681 tagaatttac gtttactggt ttgaaaacca ccaaaccaag cgtaactatg catgactggg
     1741 taaacggaga ataaaaaaag aacttgcccc ctcacattaa gcaagttagc aagctcctca
     1801 cgcaatcctc gacgactgct agattatttt agcatatttg cgtgaggact tctaaaataa
     1861 attattttgg aggtcatata catgggatta atgacaggat acaacatgac aaaagaggag
     1921 tacgaagtca cacaggctgt actgactcga ctagataagt ttgaaagtga actagaagcg
     1981 ctattgcttg atttggagcc gcttgactta tcacgattag atactgtagt tcgagacgaa
     2041 acgctaagaa tgtttaatta tctaagtcgg tcatacattc tacttcttga tacggtcgtc
     2101 tacaaagata cgctagaaga agatatcgcc tgtggaatcg tgaagattga cggccaatca
     2161 ctagacgaag aagaataaaa actagccttg cttgttataa aaaatgttga ctatttggga
     2221 taatactcga aaaacatcaa gattagccta cttccatttt ggggtaggct ttctttttgc
     2281 gagcagagca atatgctagc atgttgcgat attttgaatg acccctacca aaaatactgg
     2341 taggagtcgt tcaaattttt ggggttcccc caatcccact gctgttattt tttgctttta
     2401 ctgttgaaat ttgcttaatt tcttcctgtg tttcggaaga gcataaggga aactgggggg
     2461 cacacaaggt gccccgcctt gcagtgcaag gctttcccgt ggggtacact ttcgttcttc
     2521 gctagcactt tactagcagg tactttcagg taaactagca catttttagc actcactagc
     2581 aggtatctag cacccaaact agcagattcc tagcagtgac tagcaggttt ttagcaccaa
     2641 attagcacat tactagcacc ggctagcaga aagctagcac gttcactaat ttcaggcagt
     2701 cgttatttaa acggtttttt gacggaggga aatttggagt ttctgcctta gcagaaatgg
     2761 aaagtttgac cgtaggcaat cggctaagta cgagattggg aagcctaaag gctgaccttt
     2821 cgagtgctag acgaaagccc tcggcagagc gcacctttac gaagtaaagt atagtgcgtt
     2881 atactttact tggaagtact gccgaaatcc aaaggcaaag aacgccgcaa gaaaaaaaag
     2941 ggggggagcg ctcatgtcaa aaattgtcgc acgtatggag aaaatgaaag ttggaaactt
     3001 aagcggtatc caacggcata atcaacgaga aaccgataat cattccaatc ctgatattga
     3061 tattgagaaa tctcacttga attatgactt agtcaatcct ggttcaatca attatcggga
     3121 gaaaatcaaa caaatcattg agagccaacg aatcagtaaa cgagcggtta gaaaagacgc
     3181 agtccttgtg aacgaatgga taatcactag tgataccgct ttttttcaag agaatacaga
     3241 cacacaagct ttttttaccg atgttgtcgc gtatttctct gatcgctgcg gtcgacaaaa
     3301 tgtcgcctat gccacggtac atttagacga aaccacgccc catatgcact taggaattgt
     3361 gcctatgtac gaagggcgat tgagcagtaa acaggtgttt agtcgacaaa gtttgttaga
     3421 gattcaggaa gaactgccaa actatttgaa agaacggggc tacgctattg agcgtggact
     3481 ccgtgggagt ccgcaaaagc atttatctgt caaagaatac aaagacgttc aaaaggagct
     3541 acaggtttct aagcaggaac tagctaatca ggaacaaatt ctggcagaaa aagagagaga
     3601 actccattct tatgaacaag caatcttccg tattgaccgc cttcttgaag agaagaacga
     3661 agaagtaaag ggcaaagtcc aagcaatgaa ccaactggaa gcgaagcgaa acgaaatcat
     3721 taactctcta caaacaaccg ttttaccaga tttagcaaat gtcaaagaaa agaaatttag
     3781 tttaaacggt ttaaaatacg ttttaagcgc ttctgacctt tctgcgataa aaagtattac
     3841 caaagatttt gaaacgctta gaacgcaaaa taagcacctt aaaatcgata acaatacctt
     3901 gacacaggaa aataaagaat tgaaacaagt aaatcatgta caagaattac aaataacgga
     3961 cttaacccat gaattgaccc taaaaaaaga gcaattaaca gcgtttgaag aaaaacaact
     4021 aaactatgac cagagcgaac aaatcaaaca agaacttgat aaaacaacac aaaaagtttc
     4081 tgtgttagaa acagataata agcggttaaa agataaaatt actgacttaa cctacgactt
     4141 ttctgaacta aacgaaaaat atcaagaaat aaaattaaag tttgacgggt taaccactta
     4201 ccttcatgaa agactaggac aggccaaaga aaccatttta tttaccattc agcaaggagt
     4261 taagtacctt cagaacgcgc ctaaattaaa aaaatccctg gagcgatatt ccaaggatag
     4321 aacttacctc attgacgaaa aagggacatt gtttttacca ccagatacgg ggtcaaaggg
     4381 ggtaacaatg tccttaaacc cacatttcta ccccctaaaa gccccgtttt acaacagaga
     4441 aacagggtgt tatgagtttg tcggcgagta tatggctaat ggacgttcgc gcacaaatac
     4501 tttacagctg tctgaagaac aagtgctaga gaagaaagct tttacgtttg aacagtcgac
     4561 agataaaaag gcttggcaac agagtaaaaa atattcaaga gatatgtcaa aaggacgagg
     4621 tttatctcgt taaaaataat ttaattgata aattattgac acataaaaac agttaaatta
     4681 aaatatatta ataaaaatta aaaaaaagtg aggtaagcat tatgaaaaag ttgttagttc
     4741 taattttagg ggtgtctttt atctctgttg ctttaagttc aactacagaa gcaagtgcta
     4801 gagagtggga ttctatagag tgtcagccag gattttattg gaaaaaaggc gggtggactg
     4861 caggaggata tcataaagcc ggtggatttt gtatcccaat gcatacaaga tggacaaaat
     4921 agttgctaaa tttgaggtta tttcatgaaa aaatatctaa taaaaaatat attttatatt
     4981 actattcctt tagttttaag ctatataact tcttttttag ttaatattga tttgcccata
     5041 ttgataatta ttttttacgg aattttatta ttttttttaa ttccttcaga agtatatttg
     5101 ggaagta
//
